Genemap of the human genes associated with longevity

ABSTRACT

The present invention relates to the selection of a set of SNP markers for use in genome wide association studies based on linkage disequilibrium mapping. In particular, the invention relates to the fields of pharmacogenomics, diagnostics, patient therapy and the use of genetic haplotype information to predict an individual&#39;s longevity, their protection against age-related diseases and/or their response to a particular drug or drugs.

PRIORITY

The present application claims priority from U.S. provisionalapplication No. 60/691,309, Attorney Docket No. GENI-007/00US, filedJun. 17, 2005, entitled GENEMAP OF THE HUMAN GENES ASSOCIATED WITHLONGEVITY, which is incorporated herein by reference.

The contents of the submission on compact discs are incorporated hereinby reference in their entirety: A compact disc copy of the computerreadable format copy of the Sequence Listing (HOME COPY) (filename:GENI00701WO SeqList, date recorded: Jun. 19, 2006, file size 14,313,000bytes); a duplicate compact disc copy of the computer readable formatcopy of the Sequence Listing (SEARCH COPY) (filename: GENI00701WOSeqList, date recorded: Jun. 19, 2006, file size 14,313,000 bytes); anda triplicate copy of the computer readable format copy of the SequenceListing (RECORD COPY) (filename: GENI00701WO SeqList, date recorded:Jun. 19, 2006, file size 14,313,000 bytes).

FIELD OF THE INVENTION

The invention relates to the field of genomics and genetics, includinggenome analysis and the study of DNA variations. In particular, theinvention relates to the fields of pharmacogenomics, diagnostics,patient therapy and the use of genetic haplotype information to predictan individual's longevity, their protection against age-related diseasesand/or their response to a particular drug or drugs, so that drugstailored to genetic differences of population groups may be developedand/or administered to the appropriate population.

The invention also relates to a GeneMap for longevity, which linksvariations in DNA (including both genic and non-genic regions) to anindividual's longevity and susceptibility to age-related diseases and/orresponse to a particular drug or drugs. The invention further relates tothe genes disclosed in the GeneMap (see Tables 4, 5 and 6), which arerelated to methods and reagents for detection of an individual'sincreased or decreased risk for aging related diseases by identifying atleast one polymorphism in one or a combination of the genes from theGeneMap. Also related are the candidate regions identified in Table 1,which are associated with longevity. In addition, the invention furtherrelates to nucleotide sequences of those genes including genomic DNAsequences, cDNA sequences, single nucleotide polymorphisms (SNPs),alleles and haplotypes (see Sequence Listing and Table 2, 3 and 7).

The present invention relates to the use of genes from Tables 4, 5 and6, for determining an individual's likelihood of longevity, of beingprotected against cardiovascular related diseases (e.g., hypertension,diabetes mellitus, myocardial infarction, stroke, and/or transientischemic attack), metabolic syndrome and/or other age-related diseases,and of retaining cognitive function with aging.

The invention further relates to isolated nucleic acids comprising thesenucleotide sequences and isolated polypeptides or peptides encodedthereby. Also related, are expression vectors and host cells comprisingthe disclosed nucleic acids or fragments thereof, as well as antibodiesthat bind to the encoded polypeptides or peptides.

The present invention further relates to ligands that modulate theactivity of the disclosed genes or gene products. In addition, theinvention relates to diagnostics and therapeutics for aging relateddiseases, utilizing the disclosed nucleic acids, SNPs, chromosomalregions, gene maps, polypeptides or peptides, antibodies and/or ligandsand small molecules that activate or repress relevant signaling events.

BACKGROUND OF THE INVENTION

Many studies have documented the inheritance of human longevity (McGueet al., 1993); Ljungquist et al., 1998). Studies have also documentedthat centenarians (individuals who live for 100 years or more) are morelikely than non-centenarians to have siblings who are long-lived. Inparticular, one study has shown that the siblings of centenarians havean approximately four-fold greater probability of survival to age 91than siblings of non-centenarians (Perls et al., 1998). In addition,individuals who achieve exceptional longevity, such as centenarians,tend to live the majority of their lives in excellent health,demonstrating a rapid decline only at the end of their lives (Hitt etal., 1999). Genetic studies in other species including mammals indicatethat specific genetic polymorphisms have powerful influences upon lifespan (defined by the age of the oldest member of the species). A numberof studies on non-human species indicate that a relatively few geneticpolymorphisms have a powerful influence upon the ability to achieveexceptional longevity. Many of those polymorphisms appear to play rolesin basic mechanisms of metabolism and aging.

The DNA sequences between two human genomes are 99.9% identical. Thevariations in DNA sequence between individuals can be, for example,deletions of small or large stretches of DNA, insertions of stretches ofDNA, variations in the number of repetitive DNA elements in non-codingregions, or changes in single base positions in the genome called“single nucleotide polymorphisms” (SNPs). Human DNA sequence variationsaccount for a large fraction of observed differences betweenindividuals, including susceptibility to disorders or a genetic link totraits, such as exceptional longevity.

Complex traits such as longevity are believed to involve several genesrather than single genes, as observed in rare traits. This makesdetection of any particular gene substantially more difficult than in arare trait, where a single gene mutation segregating according to aMendelian inheritance pattern is the causative mutation. Any one of themultiple interacting gene mutations involved in the etiology of acomplex and common trait will impart a lower relative risk for the traitthan will the single gene mutation involved in a simple genetic trait.Low relative risk alleles are more difficult to detect and, as a result,the success of positional cloning using linkage mapping that wasachieved for simple genetic trait genes has not been repeated forcomplex traits.

Several approaches have been proposed to discover and characterizemultiple genes in complex genetic traits. These gene discovery methodscan be subdivided into hypothesis-free disorder association studies andhypothesis-driven candidate gene or region studies. The candidate geneapproach relies on the analysis of a gene in patients who have adisorder or a genetic trait in which the gene is thought to play a role.This approach is limited in utility because it only provides for theinvestigation of genes with known functions. Although variant sequencesof candidate genes may be identified using this approach, it isinherently limited by the fact that variant sequences in other genesthat contribute to the phenotype will be necessarily missed when thetechnique is employed. A genome-wide scan (GWS) has been shown to beefficient in identifying longevity susceptibility markers, such as theAPOE gene on chromosome 19 and APOB gene on chromosome 2. In contrast tothe candidate gene approach, a GWS searches throughout the genomewithout any a priori hypothesis and consequently can identify genes thatare not obvious candidates for the complex genetic trait as well asgenes that are relevant candidates for the trait. Furthermore, it canidentify structurally important chromosomal regions that can influencethe expression of specific, trait-related genes.

Family-based linkage mapping methods were initially used for disorderlocus identification. This technique locates genes based on therelatively limited number of genetic recombination events within thefamilies used in the study, and results in large chromosomal regionscontaining hundreds of genes, any one of which could be thetrait-causing gene. Population-based, or linkage disequilibrium (LD)mapping is based on the premise that regions adjacent to a gene ofinterest are co-transmitted through the generations along with the gene.As a result, LD extends over shorter genetic regions than does linkage(Hewett et al., 2002), and can facilitate detection of genes with lowerrelative risk than family linkage mapping approaches. It also definesmuch smaller candidate regions which may contain only a few genes,making the identification of the actual trait gene much easier.

It has been estimated that a GWS that uses a general population andcase/control association (LD) analysis would require approximately700,000 SNP markers (Carlson et al., 2003). The cost of a GWS at thismarker density for a sufficient sample size for statistical power iseconomically prohibitive. The use of a founder population (geneticisolates), such as the French Canadian population of Quebec, is onesolution to the problem with LD analysis. The French Canadian populationin Quebec (Quebec Founder Population—QFP) provides one of the bestresources in the world for gene discovery based on its high levels ofgenetic sharing and genetic homogeneity. By combining DNA collected fromthe QFP, high throughput genotyping capabilities and proprietaryalgorithms for genetic analysis, a comprehensive genome-wide associationstudy is facilitated. The present invention relates specifically to aset of longevity-related genes (GeneMap) and targets which presentattractive points of therapeutic intervention for aging-associateddiseases.

In view of the foregoing, identifying susceptibility genes associatedwith longevity and elucidating their respective biochemical pathwayswill facilitate the development of effective treatments foraging-associated diseases. This will also lead to the identification ofdiagnostic markers, which will predict the propensity for any suchdisease and allow therapeutic intervention before such disease occurs.The identification of genetic markers associated with longevity willlead to the development of effective therapeutic interventions for amuch greater proportion of the individuals affected by aging-associateddiseases. Knowledge of longevity-associated polymorphisms not onlyprovides the benefit of predicting individual longevity, but alsoprovides the ability to predict the likelihood of aging-associateddiseases. The present invention satisfies this need and provides relatedadvantages as well.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1. Method employed by the inventors to permit the identification ofgenes predisposing to a particular genetic trait, such as longevity. Themethod can be applied for any given trait and the end result is theconstruction of a GeneMap for a particular trait or disorder. Briefly, agenetically heritable disorder or trait is selected followed by thepreparation of an in-depth literature review on the prevalence,phenotypes, and available treatments (if relevant) of that trait. Theliterature review includes a list and description of candidate genes andregions associated with the trait. A clinical specialist in the field ofthe genetic trait is consulted for the definition of phenotype.Inclusion and exclusion criteria are then set and a study protocol isprepared. IRB and ethical approval are sought prior to patientrecruitment. A network of physicians is required to recruit thenecessary cases and controls for the study from the Quebec FounderPopulation. Individuals (cases and controls) are then recruited and DNAextraction and dosage is performed from the blood samples obtained.Samples are pooled into several cases and control pools. A GWS isperformed on the pooled case and control samples using, as a minimum,the marker density determined from a study of linkage disequilibrium inthe Quebec Founder Population; a study that led to the formulation ofthe Quebec linkage disequilibrium map (QLDM, a proprietary map ofGenizon Biosciences Inc.). The results from the GWS genotyping areanalyzed and candidate regions are selected for confirmatory mappingfollowed by fine mapping at a higher marker density in individual caseand control samples in order to validate and/or refine the signal. Thegene content of the candidate regions is analyzed and characterized. Therepresentative haplotypes are then selected and sequenced. Oncepolymorphisms are identified by sequencing efforts the frequencies ofgenotypes and haplotypes in individual cases and controls are analyzedin a similar manner as for the GWS and fine mapping data. Ultrafinemapping is performed on all the samples to identify the polymorphismsthat are most associated with the trait phenotype as part of the searchfor the actual DNA polymorphisms that confer susceptibility to thetrait. The genes found associated with the trait, are then corroboratedin a different population. The corroborated genes are used for theconstruction of a GeneMap.

DESCRIPTION OF THE FILES CONTAINED ON THE CD-R

The contents of the submission on compact discs are incorporated hereinby reference in their entirety: A compact disc copy of the computerreadable format copy of the Sequence Listing (HOME COPY) (filename:GENI00701WO SeqList, date recorded: Jun. 19, 2006, file size 14,313,000bytes); a duplicate compact disc copy of the computer readable formatcopy of the Sequence Listing (SEARCH COPY) (filename: GENI00701WOSeqList, date recorded: Jun. 19, 2006, file size 14,313,000 bytes); anda triplicate copy of the computer readable format copy of the SequenceListing (RECORD COPY) (filename: GENI00701WO SeqList, date recorded:Jun. 19, 2006, file size 14,313,000 bytes).

The CD-R labeled “GeneMap of the Human Gene Associated with Longevity”contains the following one file of sequence listing. Each electroniccopy of the sequence listing was created on Jun. 19, 2006 with a filesize of 14,313 KB. The file name is as follows: GENI00701WO SeqList

DEFINITIONS

Throughout the description of the present invention, several terms areused that are specific to the science of this field. For the sake ofclarity and to avoid any misunderstanding, these definitions areprovided to aid in the understanding of the specification and claims:

Allele: One of a pair, or series, of forms of a gene or non-genic regionthat occur at a given locus in a chromosome. Alleles are symbolized withthe same basic symbol (e.g., B for dominant and b for recessive; B1, B2,Bn for n additive alleles at a locus). In a normal diploid cell thereare two alleles of any one gene (one from each parent), which occupy thesame relative position (locus) on homologous chromosomes. Within apopulation there may be more than two alleles of a gene. See multiplealleles. SNPs also have alleles, i.e., the two (or more) nucleotidesthat characterize the SNP

Amplification of nucleic acids: refers to methods such as polymerasechain reaction (PCR), ligation amplification (or ligase chain reaction,LCR) and amplification methods based on the use of Q-beta replicase.These methods are well known in the art and are described, for example,in U.S. Pat. Nos. 4,683,195 and 4,683,202. Reagents and hardware forconducting PCR are commercially available. Primers useful for amplifyingsequences from the trait region, are preferably complementary to, andpreferably hybridize specifically to, sequences in the trait region orin regions that flank a target region therein. Genes from Tables 4, 5and 6 generated by amplification may be sequenced directly.Alternatively, the amplified sequence(s) may be cloned prior to sequenceanalysis.

Antigenic component: is a moiety that binds to its specific antibodywith sufficiently high affinity to form a detectable antigen-antibodycomplex.

Antibodies: refer to polyclonal and/or monoclonal antibodies andfragments thereof, and immunologic binding equivalents thereof, that canbind to proteins and fragments thereof or to nucleic acid sequences fromthe trait region, particularly from the trait gene products or a portionthereof. The term antibody is used both to refer to a homogeneousmolecular entity, or a mixture such as a serum product made up of aplurality of different molecular entities. Proteins may be preparedsynthetically in a protein synthesizer and coupled to a carrier moleculeand injected over several months into rabbits. Rabbit sera are testedfor immunoreactivity to the protein or fragment. Monoclonal antibodiesmay be made by injecting mice with the proteins, or fragments thereof.Monoclonal antibodies will be screened by ELISA and tested for specificimmunoreactivity with protein or fragments thereof (Harlow et al. 1988,Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory, ColdSpring Harbor, N.Y.). These antibodies will be useful in assays as wellas therapeutics.

Associated allele: refers to an allele at a polymorphic locus that isassociated with a particular phenotype of interest, e.g., apredisposition to a trait (e.g., longevity) or a particular drugresponse.

cDNA: refers to complementary or copy DNA produced from an RNA templateby the action of RNA-dependent DNA polymerase (reverse transcriptase).Thus, a cDNA clone is a duplex DNA sequence complementary to an RNAmolecule of interest, included in a cloning vector or amplified by PCR.This term includes genes from which the intervening sequences have beenremoved.

cDNA library: refers to a collection of recombinant DNA moleculescontaining cDNA inserts that together comprise essentially all of theexpressed genes of an organism or tissue. A cDNA library can be preparedby methods known to one skilled in the art (see, e.g., Cowell andAustin, 1997, “DNA Library Protocols,” Methods in Molecular Biology).Generally, RNA is first isolated from the cells of the desired organism,and the RNA is used to prepare cDNA molecules.

Cloning: refers to the use of recombinant DNA techniques to insert aparticular gene or other DNA sequence into a vector molecule. In orderto successfully clone a desired gene, it is necessary to use methods forgenerating DNA fragments, for joining the fragments to vector molecules,for introducing the composite DNA molecule into a host cell in which itcan replicate, and for selecting the clone having the target gene fromamongst the recipient host cells.

Cloning vector: refers to a plasmid or phage DNA or other DNA moleculethat is able to replicate in a host cell. The cloning vector istypically characterized by one or more endonuclease recognition sites atwhich such DNA sequences may be cleaved in a determinable fashionwithout loss of an essential biological function of the DNA, and whichmay contain a selectable marker suitable for use in the identificationof cells containing the vector.

Coding sequence or a protein-coding sequence: is a polynucleotidesequence capable of being transcribed into mRNA and/or capable of beingtranslated into a polypeptide or peptide. The boundaries of the codingsequence are typically determined by a translation start codon at the5′-terminus and a translation stop codon at the 3′-terminus.

Complement of a nucleic acid sequence: refers to the antisense sequencethat participates in Watson-Crick base-pairing with the originalsequence.

Trait region (can also be referred to as a disorder region, such asage-related disorder for the longevity trait): refers to the portions ofthe human chromosomes displayed in Table 1 bounded by the markers fromTable 1.

Trait-associated nucleic acid or polypeptide sequence: refers to anucleic acid sequence that maps to a region of Table 1 or thepolypeptides encoded therein (Table 2, 3 and 7 SNPs, nucleic acids, andpolypeptides). For nucleic acids, this encompasses sequences that areidentical or complementary to the gene sequences from Tables 4, 5 and 6,as well as sequence-conservative, function-conservative, andnon-conservative variants thereof. For polypeptides, this encompassessequences that are identical to the polypeptide, as well asfunction-conservative and non-conservative variants thereof. Includedare the alleles of naturally-occurring polymorphisms causative oflongevity such as, but not limited to, alleles that cause alteredexpression of genes of Tables 4, 5 and 6 and alleles that cause alteredprotein levels or stability (e.g., decreased levels, increased levels,expression in an inappropriate tissue type, increased stability, anddecreased stability).

Expression vector: refers to a vehicle or plasmid that is capable ofexpressing a gene that has been cloned into it, after transformation orintegration in a host cell. The cloned gene is usually placed under thecontrol of (i.e., operably linked to) a regulatory sequence.

Function-conservative variants: are those in which a change in one ormore nucleotides in a given codon position results in a polypeptidesequence in which a given amino acid residue in the polypeptide has beenreplaced by a conservative amino acid substitution.Function-conservative variants also include analogs of a givenpolypeptide and any polypeptides that have the ability to elicitantibodies specific to a designated polypeptide.

Founder population: also called a population isolate, this is a largenumber of people who have mostly descended, in genetic isolation fromother populations, from a much smaller number of people who lived manygenerations ago.

Gene: refers to a DNA sequence that encodes through its template ormessenger RNA a sequence of amino acids characteristic of a specificpeptide, polypeptide, or protein. The term “gene” also refers to a DNAsequence that encodes an RNA product. The term gene as used herein withreference to genomic DNA includes intervening, non-coding regions, aswell as regulatory regions, and can include 5′ and 3′ ends. A genesequence is wild-type if such sequence is usually found in individualsunaffected by the trait or condition of interest, e.g., longevity.However, environmental factors and other genes can also play animportant role in the ultimate determination of the genetic trait. Inthe context of complex traits involving multiple genes (oligogenictraits), the wild type, or normal sequence can also be associated with ameasurable risk or susceptibility, receiving its reference status basedon its frequency in the general population.

GeneMaps are defined as groups of gene(s) that are directly orindirectly involved in at least one phenotype of a trait, e.g.,longevity. As such, GeneMaps enable the development of synergisticdiagnostic products, creating “theranostics”.

Genotype: Set of alleles at a specified locus or loci.

Haplotype: The allelic pattern of a group of (usually contiguous) DNAmarkers or other polymorphic loci along an individual chromosome ordouble helical DNA segment. Haplotypes identify individual chromosomesor chromosome segments. The presence of shared haplotype patterns amonga group of individuals implies that the locus defined by the haplotypehas been inherited, identical by descent (IBD), from a common ancestor.Detection of identical by descent haplotypes is the basis of linkagedisequilibrium (LD) mapping. Haplotypes are broken down through thegenerations by recombination and mutation. In some instances, a specificallele or haplotype may be associated with susceptibility to a trait orcondition of interest, e.g., longevity. In other instances, an allele orhaplotype may be associated with a decrease in susceptibility to a traitor condition of interest, i.e., a protective sequence (see Table 7 forthe significant haplotypes associated with longevity).

Host: includes prokaryotes and eukaryotes. The term includes an organismor cell that is the recipient of an expression vector (e.g.,autonomously replicating or integrating vector).

Hybridizable: nucleic acids are hybridizable to each other when at leastone strand of the nucleic acid can anneal to another nucleic acid strandunder defined stringency conditions. In some embodiments, hybridizationrequires that the two nucleic acids contain at least 10 substantiallycomplementary nucleotides; depending on the stringency of hybridization,however, mismatches may be tolerated. The appropriate stringency forhybridizing nucleic acids depends on the length of the nucleic acids andthe degree of complementarity, and can be determined in accordance withthe methods described herein.

Identity by descent (IBD): Identity among DNA sequences for differentindividuals that is due to the fact that they have all been inheritedfrom a common ancestor. LD mapping identifies IBD haplotypes as thelikely location of trait genes shared by a group of cases.

Identity: as known in the art, is a relationship between two or morepolypeptide sequences or two or more polynucleotide sequences, asdetermined by comparing the sequences. In the art, identity also meansthe degree of sequence relatedness between polypeptide or polynucleotidesequences, as the case may be, as determined by the match betweenstrings of such sequences. Identity and similarity can be readilycalculated by known methods, including but not limited to thosedescribed in A. M. Lesk (ed), 1988, Computational Molecular Biology,Oxford University Press, NY; D. W. Smith (ed), 1993, Biocomputing.Informatics and Genome Projects, Academic Press, NY; A. M. Griffin andH. G. Griffin, H. G (eds), 1994, Computer Analysis of Sequence Data,Part 1, Humana Press, NJ; G. von Heinje, 1987, Sequence Analysis inMolecular Biology, Academic Press; and M. Gribskov and J. Devereux(eds), 1991, Sequence Analysis Primer, M Stockton Press, NY; H. Carilloand D. Lipman, 1988, SIAM J. Applied Math., 48:1073.

Immunogenic component: is a moiety that is capable of eliciting ahumoral and/or cellular immune response in a host animal.

Isolated nucleic acids: are nucleic acids separated away from othercomponents (e.g., DNA, RNA, and protein) with which they are associated(e.g., as obtained from cells, chemical synthesis systems, or phage ornucleic acid libraries). Isolated nucleic acids are at least 60% free,preferably 75% free, and most preferably 90% free from other associatedcomponents. In accordance with the present invention, isolated nucleicacids can be obtained by methods described herein, or other establishedmethods, including isolation from natural sources (e.g., cells, tissues,or organs), chemical synthesis, recombinant methods, combinations ofrecombinant and chemical methods, and library screening methods.

Isolated polypeptides or peptides: are those that are separated fromother components (e.g., DNA, RNA, and other polypeptides or peptides)with which they are associated (e.g., as obtained from cells,translation systems, or chemical synthesis systems). In a preferredembodiment, isolated polypeptides or peptides are at least 10% pure;more preferably, 80% or 90% pure. Isolated polypeptides and peptidesinclude those obtained by methods described herein or other establishedmethods, including isolation from natural sources (e.g., cells, tissues,or organs), chemical synthesis, recombinant methods, or combinations ofrecombinant and chemical methods. Proteins or polypeptides referred toherein as recombinant are proteins or polypeptides produced by theexpression of recombinant nucleic acids. A portion as used herein withregard to a protein or polypeptide refers to fragments of that proteinor polypeptide. The fragments can range in size from 5 amino acidresidues to all but one residue of the entire protein sequence. Thus, aportion or fragment can be at least 5, 5-50, 50-100, 100-200, 200-400,400-800, or more consecutive amino acid residues of a protein orpolypeptide.

Linkage disequilibrium (LD): a statistical association betweenparticular alleles at separate but linked loci, normally the result of aparticular ancestral haplotype being common in the population studied.LD can also be defined as the situation in which the alleles for two ormore loci do not occur together in individuals sampled from a populationat frequencies predicted by the product of their individual allelefrequencies. In other words, markers that are in LD do not followMendel's second law of independent random segregation. LD can be causedby any of several demographic or population artefacts as well as by thepresence of genetic linkage between markers. However, when theseartefacts are controlled and eliminated as sources of LD, then LDresults directly from the fact that the loci involved are located closeto each other on the same chromosome so that specific combinations ofalleles for different markers (haplotypes) are inherited together.Markers that are in high LD can be assumed to be located near each otherand a marker or haplotype that is in high LD with a genetic trait can beassumed to be located near the gene that affects that trait. Thephysical proximity of markers can be measured in family studies where itis called linkage or in population studies where it is called linkagedisequilibrium.

LD mapping: population based gene mapping, which locates trait genes byidentifying regions of the genome where haplotypes or marker variationpatterns are shared statistically more frequently among cases comparedto healthy controls. This method is based upon the assumption that manyof the cases will have inherited an allele associated with the traitfrom a common ancestor (IBD), and that this allele will be in LD withthe trait gene.

Locus: a specific position along a chromosome or DNA sequence. Dependingupon context, a locus could be a gene, a marker, a chromosomal band or aspecific sequence of one or more nucleotides.

Minor allele frequency (MAF): the population frequency of one of thealleles for a given polymorphism, which is equal or less than 50%. Thesum of the MAF and the Major allele frequency equals one.

Markers: an identifiable DNA sequence that is variable (polymorphic) fordifferent individuals within a population. These sequences facilitatethe study of inheritance of a trait or a gene. Such markers are used inmapping the order of genes along chromosomes and in following theinheritance of particular genes; genes closely linked to the marker orin LD with the marker will generally be inherited with it. Two types ofmarkers are commonly used in genetic analysis, microsatellites and SNPs.

Microsatellite: DNA of eukaryotic cells comprising a repetitive, shortsequence of DNA that is present as tandem repeats and in highly variablecopy number, flanked by sequences unique to that locus.

Mutant sequence: a sequence that differs from one or more wild-typesequences. For example, a nucleic acid from a gene listed in Tables 4, 5and 6 containing a particular allele of a single nucleotide polymorphismmay be a mutant sequence. In some cases, the individual carrying thisallele has increased susceptibility toward the trait, or condition ofinterest. In other cases, the mutant sequence might also refer to anallele that decreases the susceptibility toward a trait or condition ofinterest and thus acts in a protective manner. The term mutation mayalso be used to describe a specific allele at a polymorphic locus.

Non-conservative variants: are those in which a change in one or morenucleotides in a given codon position results in a polypeptide sequencein which a given amino acid residue in a polypeptide has been replacedby a non-conservative amino acid substitution. Non-conservative variantsalso include polypeptides comprising non-conservative amino acidsubstitutions.

Nucleic acid or polynucleotide: purine- and pyrimidine-containingpolymers of any length, either polyribonucleotides orpolydeoxyribonucleotide or mixed polyribo polydeoxyribonucleotides. Thisincludes single- and double-stranded molecules, i.e., DNA-DNA, DNA-RNAand RNA-RNA hybrids, as well as protein nucleic acids (PNA) formed byconjugating bases to an amino acid backbone. This also includes nucleicacids containing modified bases.

Nucleotide: consist of a ribose or deoxyribose sugar joined to a purineor pyrimidine base and to a phosphate group and that are the basicstructural units of RNA and DNA. For its incorporation in DNA,nucleotides need to possess three phosphate esters but they areconverted into monoesters in the process of incorporation.

Operably linked: means that the promoter controls the initiation ofexpression of the gene. A promoter is operably linked to a sequence ofproximal DNA if upon introduction into a host cell the promoterdetermines the transcription of the proximal DNA sequence(s) into one ormore species of RNA. A promoter is operably linked to a DNA sequence ifthe promoter is capable of initiating transcription of that DNAsequence.

Ortholog: denotes a gene or polypeptide obtained from one species thathas homology to an analogous gene or polypeptide from a differentspecies.

Paralog: denotes a gene or polypeptide obtained from a given speciesthat has homology to a distinct gene or polypeptide from that samespecies.

Phenotype: any visible, detectable or otherwise measurable property ofan organism such as symptoms of, or susceptibility to, a disorder ortrait.

Polymorphism: occurrence of two or more alternative genomic sequences oralleles between or among different genomes or individuals at a singlelocus. A polymorphic site thus refers specifically to the locus at whichthe variation occurs. In some cases, an individual carrying a particularallele of a polymorphism has an increased or decreased susceptibilitytoward a trait or condition of interest.

Portion and fragment: are synonymous. A portion as used with regard to anucleic acid or polynucleotide refers to fragments of that nucleic acidor polynucleotide. The fragments can range in size from 8 nucleotides toall but one nucleotide of the entire gene sequence. Preferably, thefragments are at least about 8 to about 10 nucleotides in length; atleast about 12 nucleotides in length; at least about 15 to about 20nucleotides in length; at least about 25 nucleotides in length; or atleast about 35 to about 55 nucleotides in length.

Probe or primer: refers to a nucleic acid or oligonucleotide that formsa hybrid structure with a sequence in a target region of a nucleic aciddue to complementarity of the probe or primer sequence to at least oneportion of the target region sequence.

Protein and polypeptide: are synonymous. Peptides are defined asfragments or portions of polypeptides, preferably fragments or portionshaving at least one functional activity (e.g., proteolysis, adhesion,fusion, antigenic, or intracellular activity) as the completepolypeptide sequence.

Recombinant nucleic acids: nuclei acids which have been produced byrecombinant DNA methodology, including those nucleic acids that aregenerated by procedures which rely upon a method of artificialreplication, such as the polymerase chain reaction (PCR) and/or cloninginto a vector using restriction enzymes. Portions of recombinant nucleicacids which code for polypeptides can be identified and isolated by, forexample, the method of M. Jasin et al., U.S. Pat. No. 4,952,501.

Regulatory sequence: refers to a nucleic acid sequence that controls orregulates expression of structural genes when operably linked to thosegenes. These include, for example, the lac systems, the trp system,major operator and promoter regions of the phage lambda, the controlregion of fd coat protein and other sequences known to control theexpression of genes in prokaryotic or eukaryotic cells. Regulatorysequences will vary depending on whether the vector is designed toexpress the operably linked gene in a prokaryotic or eukaryotic host,and may contain transcriptional elements such as enhancer elements,termination sequences, tissue-specificity elements and/or translationalinitiation and termination sites.

Sample: as used herein refers to a biological sample, such as, forexample, tissue or fluid isolated from an individual or animal(including, without limitation, plasma, serum, cerebrospinal fluid,lymph, tears, nails, hair, saliva, milk, pus, and tissue exudates andsecretions) or from in vitro cell culture-constituents, as well assamples obtained from, for example, a laboratory procedure.

Single nucleotide polymorphism (SNP): variation of a single nucleotide.This includes the replacement of one nucleotide by another and deletionor insertion of a single nucleotide. Typically, SNPs are biallelicmarkers although tri- and tetra-allelic markers also exist. For example,SNP A\C may comprise allele C or allele A (Table 2, 3 and 7). Thus, anucleic acid molecule comprising SNP A\C may include a C or A at thepolymorphic position. For a combination of SNPs, the term “haplotype” isused, e.g. the genotype of the SNPs in a single DNA strand that arelinked to one another. In certain embodiments, the term “haplotype” isused to describe a combination of SNP alleles, e.g., the alleles of theSNPs found together on a single DNA molecule. In specific embodiments,the SNPs in a haplotype are in linkage disequilibrium with one another.

Sequence-conservative: variants are those in which a change of one ormore nucleotides in a given codon position results in no alteration inthe amino acid encoded at that position (i.e., silent mutation).

Substantially homologous: a nucleic acid or fragment thereof issubstantially homologous to another if, when optimally aligned (withappropriate nucleotide insertions and/or deletions) with the othernucleic acid (or its complementary strand), there is nucleotide sequenceidentity in at least 60% of the nucleotide bases, usually at least 70%,more usually at least 80%, preferably at least 90%, and more preferablyat least 95-98% of the nucleotide bases. Alternatively, substantialhomology exists when a nucleic acid or fragment thereof will hybridize,under selective hybridization conditions, to another nucleic acid (or acomplementary strand thereof). Selectivity of hybridization exists whenhybridization which is substantially more selective than total lack ofspecificity occurs. Typically, selective hybridization will occur whenthere is at least about 55% sequence identity over a stretch of at leastabout nine or more nucleotides, preferably at least about 65%, morepreferably at least about 75%, and most preferably at least about 90%(M. Kanehisa, 1984, NucL Acids Res. 11:203-213). The length of homologycomparison, as described, may be over longer stretches, and in certainembodiments will often be over a stretch of at least 14 nucleotides,usually at least 20 nucleotides, more usually at least 24 nucleotides,typically at least 28 nucleotides, more typically at least 32nucleotides, and preferably at least 36 or more nucleotides.

Wild-type gene from Tables 4, 5 and 6: refers to the reference sequence.The wild-type gene sequences from Tables 4, 5 and 6 used to identify thevariants (single nucleotide polymorphisms, alleles, and haplotypes)described in detail herein.

Technical and scientific terms used herein have the meanings commonlyunderstood by one of ordinary skill in the art to which the presentinvention pertains, unless otherwise defined. Reference is made hereinto various methodologies known to those of skill in the art.Publications and other materials setting forth such known methodologiesto which reference is made are incorporated herein by reference in theirentireties as though set forth in full. Standard reference works settingforth the general principles of recombinant DNA technology include J.Sambrook et al., 1989, Molecular Cloning: A Laboratory Manual, 2d Ed.,Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.; P. B.Kaufman et al., (eds), 1995, Handbook of Molecular and Cellular Methodsin Biology and Medicine, CRC Press, Boca Raton; M. J. McPherson (ed),1991, Directed Mutagenesis: A Practical Approach, IRL Press, Oxford; J.Jones, 1992, Amino Acid and Peptide Synthesis, Oxford SciencePublications, Oxford; B. M. Austen and O. M. R. Westwood, 1991, ProteinTargeting and Secretion, IRL Press, Oxford; D. N Glover (ed), 1985, DNACloning, Volumes I and 11; M. J. Gait (ed), 1984, OligonucleotideSynthesis; B. D. Hames and S. J. Higgins (eds), 1984, Nucleic AcidHybridization; Quirke and Taylor (eds), 1991, PCR-A Practical Approach;Harries and Higgins (eds), 1984, Transcription and Translation; R. I.Freshney (ed), 1986, Animal Cell Culture; Immobilized Cells and Enzymes,1986, IRL Press; Perbal, 1984, A Practical Guide to Molecular Cloning,J. H. Miller and M. P. Calos (eds), 1987, Gene Transfer Vectors forMammalian Cells, Cold Spring Harbor Laboratory Press; M. J. Bishop (ed),1998, Guide to Human Genome Computing, 2d Ed., Academic Press, SanDiego, Calif.; L. F. Peruski and A. H. Peruski, 1997, The Internet andthe New Biology. Tools for Genomic and Molecular Research, AmericanSociety for Microbiology, Washington, D.C. Standard reference workssetting forth the general principles of immunology include S. Sell,1996, Immunology, Immunopathology & Immunity, 5th Ed., Appleton & Lange,Publ., Stamford, Conn.; D. Male et al., 1996, Advanced Immunology, 3dEd., Times Mirror Int'l Publishers Ltd., Publ., London; D. P. Stites andA. L Terr, 1991, Basic and Clinical Immunology, 7th Ed., Appleton &Lange, Publ., Norwalk, Conn.; and A. K. Abbas et al., 1991, Cellular andMolecular Immunology, W. B. Saunders Co., Publ., Philadelphia, Pa. Anysuitable materials and/or methods known to those of skill can beutilized in carrying out the present invention; however, preferredmaterials and/or methods are described. Materials, reagents, and thelike to which reference are made in the following description andexamples are generally obtainable from commercial sources, and specificvendors are cited herein.

DETAILED DESCRIPTION OF THE INVENTION

Aging is a process in which all individuals of a species undergo aprogressive decline in vitality leading to death. In metazoans, aging atthe level of the whole organism is clearly evident. Aging of an organismrepresents the effects of entropy over time and has also been shown bymany to be genetically programmed. While the effect of genetics on lifeexpectancy is minimal across ages, this is not the case withcentenarians (a rare phenotype achieved by 1 in 10,000 individuals).Siblings of current centenarians have odds ratios of between 8 and 17 ofachieving 100 years of age, and parents of centenarians have an oddsratio of 7 for achieving ages 90-99 compared to appropriate controls.Furthermore, the offspring of long-lived parents have a significantlylower prevalence (50%) of hypertension, diabetes mellitus, myocardialinfarctions and strokes/transient ischemic attacks compared with severalage-matched control groups. In support of the inheritance of longevity,the New England Centenarian Study reported a statistically significantlinkage between a genetic locus on chromosome 4 and exceptionallongevity among siblings of centenarians.

Evidence in support of a genetic determinant for aging has been obtainedin various organisms. For instance, in the yeast Saccharomycescerevisiae, the patterns of expression of certain genes change in aspecific manner during the life span, and these changed patterns suggestthat the aging process is subject to gene regulation. Controlledexpression of the transforming gene of Harvey murine sarcoma virus(v-Ha-ras) was found to extend yeast life span (as measured by thenumber of cell divisions) nearly two-fold (Jazwinski et al., 1993). RAS1and RAS2, which are yeast homologs of the v-Ha-ras oncogene, playcentral roles in the integration of cell growth and the cell cycle inyeast. The primary role of these RAS proteins in yeast is theGTP-dependent regulation of adenylate cyclase activity. Curiously,mutations in RAS1 and RAS2 have opposite effects on yeast life span. Thedeletion of RAS1 lengthened life span while deletion of RAS2 decreasedlife span. D'mello, N. P. et al. (1994) isolated a yeast gene denotedlongevity-assurance gene-1 (LAG1). LAG1 expression is highest in youngcells and decreases as yeast cells age.

Furthermore, numerous diseases and disorders are associated with aging.Diseases which show age-dependent onset of symptoms include Alzheimer'sdisease, Pick's disease, Huntington's disease, Parkinson's disease,adult onset myotonic dystrophy, multiple sclerosis, adult onsetleukodystrophy, diabetes mellitus, arteriosclerosis, and cancer.

Patients who suffer from premature aging syndromes exhibit numerousdefects associated with more advanced age groups. Symptoms of Werner'ssyndrome include scleroderma-like skin changes, cataracts, subcutaneouscalcification, premature arteriosclerosis, and diabetes mellitus. Astriking aspect of Werner's syndrome, presumably arising from the samegenetic defect, is a dramatic shortening of the replicative life-span ofdermal fibroblasts in vitro (Faragher et al., 1993).

Scientists have also found that substantially reducing an organism'scaloric intake increases longevity in mammals. Caloric restriction alsoknown as “undernutrition without malnutrition” refers to a daily diethaving about 30 to 40% fewer calories than the typical daily diet, butwhich contains the required nutrients and vitamins to support life.Caloric restriction extends both the maximal and the average life spanof mice. In addition, preliminary studies suggest thatcalorie-restricted monkeys are healthier and tend to live longer thantheir freely fed counterparts (Mattison et al., 2003). In addition toincreasing an organism's life span, caloric restriction plays a role inpreventing or delaying many age-associated diseases and conditions, suchas heart disease, dementia, and cancer. It has been found that caloricrestriction not only slows the effects of aging on the nervous system,but studies suggest that it boosts the immune system and delays theonset of certain age-related cancers.

Mitochodria have also been implicated in age-related diseases.Mitochondria are cellular organelles often referred to as the“powerhouses” of the cell because they are the sites for cellularrespiration or energy production in the cell. Indeed, mitochondriagenerate most of the energy of the cell primarily through oxidativephosphorylation, a complex process that uses electrons generated throughoxidation of glucose and fatty acids to generate ATP. Aging mitochondriasuffer from impaired function, which is associated with a variety offunctional deficits (both physical and cognitive) and also thedevelopment of degenerative diseases. Proteins of the mitochondriaoxidative phosphorylation complex have been shown to be impaired uponaging, which leads to a higher production of reactive oxygen species(ROS) and a decrease in efficiency of energy production. Free radicalsproduced by aerobic respiration cause cumulative oxidative damageresulting in aging and cell death. The biggest impact of age-relatedincrease in ROS appears to be on somatic tissues composed ofpost-mitotic non-replicative cells including muscles, e.g., cardiac andskeletal, and nervous tissues, e.g., brain, retinal pigment epithelium.Numerous age-related changes have been reported in mitochondria. Forexample, oxidative damage to mitochondria DNA (mt DNA) increases withaging (Beckman et al., 1999) along with the oxidation of glutathione(GSH) a major intracellular antioxidant system, which plays an importantrole in protection against age-related mt DNA oxidative damage.

A relative lack of polymorphic variants associated with diseases ofaging may be one prerequisite to achieving exceptional longevity. Forexample, the absence of genetic polymorphisms among centenarians isexemplified by the rarity of the apolipoprotein E E4 allele that hasbeen associated with Alzheimer's disease and cardiovascular disease(Schachter et al., 1994). Another prerequisite to achieving exceptionallongevity may be the ability to modulate the rate of the aging process,which also appears to have a genetic component. For example, one studyhas shown that the offspring of centenarians had more favorable lipidprofile characteristics (Barzilai et al., 2001).

Thus, while there are a number of genetic studies and markers known,longevity and age-related diseases are not well understood. There isalso a continuing need in the medical arts for genetic markers oflongevity and guidance for the use of such markers. Compositions andmethods of the invention are useful for predicting the propensity forexceptional longevity in humans. Additionally, compositions and methodsof the invention are useful for predicting the propensity forage-related diseases including, but not limited to heart disease,cardiovascular disease, stroke, Alzheimer's disease, cancer, and oculardisease. Additionally, compositions and methods of the invention areuseful for indicating possible early therapeutic intervention to preventor to lessen the effects of diseases associated with aging. The presentinvention fulfills this need and provides further related advantages.

Genome Wide Association Study to Construct a GeneMap for Longevity

The present invention is based on the discovery of genes associated withlongevity. In the preferred embodiment, trait-associated loci (candidateregions; Tables 1-7) are therefore identified by the statisticallysignificant differences in allele frequencies between the cases and thecontrols. For the purpose of the present invention, 47 candidate regionsshowing a difference with a −log 10 P value of 3.0 or higher areidentified. The only previously replicated locus associated withlongevity is at 4q24-q25 (Puca et al., 2001).

The invention provides a method for the discovery of genes associatedwith longevity and the construction of a GeneMap for longevity in ahuman population, comprising the following steps (see FIG. 1 and Examplesection herein):

Step 1: Recruit Cases and Controls

In the preferred embodiment, 500 cases ascertained to be 94 years old orolder along with 500 control individuals are recruited from the QuebecFounder Population (QFP).

In another embodiment, 615 cases ascertained to be 94 years old or olderalong with 615 control individuals, ascertained to be 65 years old oryounger, are recruited from the Quebec Founder Population (QFP).

In another embodiment, the present invention is performed as a whole orpartially with DNA samples from individuals of another founderpopulation than the Quebec population or from the general population.

Step 2: DNA Extraction and Dosage

Any sample comprising cells or nucleic acids from patients or controlsmay be used. Preferred samples are those easily obtained from thepatient or control. Such samples include, but are not limited to blood,peripheral lymphocytes, buccal swabs, epithelial cell swabs, nails,hair, bronchoalveolar lavage fluid, sputum, or other body fluid ortissue obtained from an individual.

In the preferred embodiment, DNA is extracted from such samples in thequantity and quality necessary to perform the invention usingconventional DNA extraction and dosage techniques. The present inventionis not linked to any DNA extraction or dosage platform in particular.

The extracted DNA from case and control samples from recruitedindividuals is pooled together in various pools. Pools are designed tosegregate cases from controls, and males from females.

In the preferred embodiment, proband pools consist of DNA extracted fromrecruited cases and control pools consist of DNA extracted from controlindividuals. The probands are also segregated according to their age atthe time of recruitment and the proband females are further separated intwo groups, those who failed a cognitive test and those who passed thetest. Two proband male pools contain preferably 53-74 individuals,separated by age group. One proband female pool contains the 71 femaleswho failed a cognitive test whereas the 7 remaining pools consist of43-80 proband females who passed the test, separated by age group.Preferably, the ten (10) control pools consist of 8 pools of 61 femalesamples and 2 pools of 63-64 male samples.

In the preferred embodiment proband samples of 615 cases (127 males and488 females) are used to construct the case pools and 615 controls (127males and 488 females) are used to construct the control pools.

Step 3: Genotype the Proband and Control Pools

In the preferred embodiment, assay specific and/or locus-specific and/orallele-specific oligonucleotides for every SNP marker of the presentinvention (Table 2) are organized onto one or more arrays. The genotypeat each SNP locus is revealed by hybridizing short PCR fragmentscomprising each SNP locus onto these arrays. The arrays permit ahigh-throughput genome wide association study using DNA samples fromindividuals of the Quebec founder population. Such assay-specific and/orlocus-specific and/or allele-specific oligonucleotides necessary forscoring each SNP of the present invention are preferably organized ontoa solid support. Such supports can be arrayed on wafers, glass slides,beads or any other type of solid support.

In another embodiment, the assay-specific and/or locus-specific and/orallele-specific oligonucleotides are not organized onto a solid supportbut are still used as a whole, in panels or one by one. The presentinvention is therefore not linked to any genotyping platform inparticular.

In another embodiment, one or more portions of the SNPs maps (publiclyavailable maps, proprietary maps from Perlegen Sciences, Inc. (MountainView, Calif., USA), and our own proprietary QLDM map) are used to screenthe whole genome, a subset of chromosomes, a chromosome, a subset ofgenomic regions or a single genomic region.

Step 4: Exclude the Markers that Did not Pass the Quality Control of theAssay

Preferably, the quality controls consist of, but are not limited to, thefollowing criteria: eliminate SNPs that are non-polymorphic in theQuebec founder population or have ≦10% minor allele frequency (MAF).

Step 5: Perform the Genetic Analysis on the Results Obtained

In the preferred embodiment, genetic analysis is performed on all thegenotypes from step 3.

In another embodiment, genetic analysis is performed on a total of248,535 SNPs.

In one embodiment, the data analysis compares the relative fluorescenceintensities of features corresponding to the reference allele of a givenSNP with those corresponding to the alternate allele, to calculate ap-hat value. The latter is proportional to the fluorescence signal fromperfect match features for the reference allele divided by the sum offluorescence signals from perfect match features for the reference plusthe alternate alleles. P-hat assumes values close to 1 (typically 0.9)for pure reference samples and close to 0 (typically 0.1) for purealternate samples, and can be used as a measured estimate of thereference allele frequency of a SNP in a DNA pool. The differencebetween case and control pools, delta p-hat, is calculated using theweighted average of case and control p-hats. Delta p-hat is a reliableestimate of the allele frequency difference between the cases andcontrols.

In yet another embodiment, the data is analyzed according to the p-hatvalue obtained from the previous embodiment on each pool. Single markerP values are calculated for all markers within the genome wide scan mapas described in Example 3 herein, using the p-hat value.

In another embodiment, the combined P values across multi-marker slidingwindows are calculated after the method of Fisher (described in Example3 herein).

Step 6: Fine Mapping and Confirmatory Mapping

In this step, the candidate regions that were identified by step 5 arefurther mapped and confirmed for the purpose of refinement andvalidation.

In the preferred embodiment, the cases and controls are individuallygenotyped to confirm the candidate regions. The confirmed candidateregions are processed by fine mapping to refine the candidate regions.

In the preferred embodiment, this fine mapping is performed with adensity of genetic markers higher than in the genome wide scan (step 3)using any genotyping platform available in the art. Such fine mappingcan also be performed with fewer genetic markers than in the GWS. Suchfine mapping can be, but is not limited to, typing the allele via anallele-specific elongation assay that is then ligated to alocus-specific oligonucleotide. Such assays can be performed directly onthe genomic DNA at a highly multiplex level and the products can beamplified using universal oligonucleotides. For each candidate region,the density of genetic markers can be, but is not limited to, a set ofSNP markers with an average inter-marker distance of 1-4 Kb distributedover about 400 Kb to 1 Mb, roughly centered at the highest point of theGWS association. The preferred samples are those obtained from longevitysamples including the ones used for the GWS.

In the preferred embodiment, the genetic analysis of the resultsobtained using haplotype information (available after confirmatorymapping of individual samples, see Examples section herein) as well assingle-marker association (as performed as in step 5, described herein)are performed as described herein (see Example section). The candidateregions that are validated and confirmed after this analysis proceed toa gene mining step described in Example 5, herein, to characterize theirmarker and genetic content.

Step 7: SNP and DNA Polymorphism Discovery

In the preferred embodiment, all the candidate genes and regionsidentified in step 6 are sequenced for polymorphism identification.

In another embodiment, the entire region, including all introns, issequenced to identify all polymorphisms.

In yet another embodiment, the candidate genes are prioritized forsequencing, and only functional gene elements (promoters, exons andsplice sites) are sequenced.

In yet another embodiment, previously identified polymorphisms in thecandidate regions can also be used. For example, SNPs from dbSNP,Perlegen Sciences, Inc., or others can also be used rather thanresequencing the candidate regions to identify polymorphisms.

The discovery of SNPs and DNA polymorphisms generally comprises a stepconsisting of determining the major haplotypes in the region to besequenced. The preferred samples are selected according to whichhaplotypes contribute to the association signal observed in the regionto be sequenced. The purpose is to select a set of samples that coversall the major haplotypes in the given region. Each major haplotype ispreferably analyzed in at least a few individuals.

Any analytical procedure may be used to detect the presence or absenceof variant nucleotides at one or more polymorphic positions of theinvention. In general, the detection of allelic variation requires amutation discrimination technique, optionally an amplification reactionand optionally a signal generation system. Any means of mutationdetection or discrimination may be used. For instance, DNA sequencing,scanning methods, hybridization, extension based methods, incorporationbased methods, restriction enzyme-based methods and ligation-basedmethods may be used in the methods of the invention.

Sequencing methods include, but are not limited to, direct sequencing,and sequencing by hybridization. Scanning methods include, but are notlimited to, protein truncation test (PTT), single-strand conformationpolymorphism analysis (SSCP), denaturing gradient gel electrophoresis(DGGE), temperature gradient gel electrophoresis (TGGE), cleavage,heteroduplex analysis, chemical mismatch cleavage (CMC), and enzymaticmismatch cleavage. Hybridization-based methods of detection include, butare not limited to, solid phase hybridization such as dot blots,multiple allele specific diagnostic assay (MASDA), reverse dot blots,and oligonucleotide arrays (DNA Chips). Solution phase hybridizationamplification methods may also be used, such as Taqman. Extension basedmethods include, but are not limited to, amplification refractionmutation systems (ARMS), amplification refractory mutation systems(ALEX), and competitive oligonucleotide priming systems (COPS).Incorporation based methods include, but are not limited to,mini-sequencing and arrayed primer extension (APEX). Restrictionenzyme-based detection systems include, but are not limited to,restriction site generating PCR. Lastly, ligation based detectionmethods include, but are not limited to, oligonucleotide ligation assays(OLA). Signal generation or detection systems that may be used in themethods of the invention include, but are not limited to, fluorescencemethods such as fluorescence resonance energy transfer (FRET),fluorescence quenching, fluorescence polarization as well as otherchemiluminescence, electrochemiluminescence, Raman, radioactivity,colometric methods, hybridization protection assays and massspectrometry methods. Further amplification methods include, but are notlimited to self sustained replication (SSR), nucleic acid sequence basedamplification (NASBA), ligase chain reaction (LCR), strand displacementamplification (SDA) and branched DNA (B-DNA).

Step 8: Ultrafine Mapping

This step further maps the candidate regions and genes confirmed in theprevious step to identify and validate the responsible polymorphismsassociated with longevity in the human population.

In a preferred embodiment, the discovered SNPs and polymorphisms of step7 are ultrafine mapped at a higher density of markers than the finemapping described herein using the same technology described in step 6.

Step 9: GeneMap Construction

The confirmed variations in DNA (including both genic and non-genicregions) are used to build a GeneMap for longevity disorder. The genecontent of this GeneMap is described in more detail below. Such GeneMapcan be used for other methods of the invention comprising the diagnosticmethods described herein, the susceptibility to longevity, the responseto a particular drug, the efficacy of a particular drug, the screeningmethods described herein and the treatment methods described herein.

As is evident to one of ordinary skill in the art, all of the abovesteps or the steps of FIG. 1 do not need to be performed, or performedin a given order to practice or use the SNPs, genomic regions, genes,proteins, etc. in the methods of the invention.

Genes from the GeneMap

In the preferred embodiment the GeneMap consists of genes and targets,in a variety of combinations, identified from the candidate regionslisted in Table 1. In the preferred embodiment, all genes from Tables 4,5 and 6 are present in the GeneMap.

Nucleic Acid Sequences

The nucleic acid sequences of the present invention may be derived froma variety of sources including DNA, cDNA, synthetic DNA, synthetic RNA,derivatives, mimetics or combinations thereof. Such sequences maycomprise genomic DNA, which may or may not include naturally occurringintrons, genic regions, nongenic regions, and regulatory regions.Moreover, such genomic DNA may be obtained in association with promoterregions or poly (A) sequences. The sequences, genomic DNA, or cDNA maybe obtained in any of several ways. Genomic DNA can be extracted andpurified from suitable cells by means well known in the art.Alternatively, mRNA can be isolated from a cell and used to produce cDNAby reverse transcription or other means. The nucleic acids describedherein are used in certain embodiments of the methods of the presentinvention for production of RNA, proteins or polypeptides, throughincorporation into cells, tissues, or organisms. In one embodiment, DNAcontaining all or part of the coding sequence for the genes described inTables 4, 5 and 6, or the SNP markers described in Tables 2, 3 and 7, isincorporated into a vector for expression of the encoded polypeptide insuitable host cells. The invention also comprises the use of thenucleotide sequence of the nucleic acids of this invention to identifyDNA probes for the genes described in Tables 4, 5 and 6 or the SNPmarkers described in Table 2, 3 or 7, PCR primers to amplify the genesdescribed in Tables 4, 5 and 6 or the SNP markers described in Tables 2,3 and 7, nucleotide polymorphisms in the genes described in Tables 4, 5and 6, and regulatory elements of the genes described in Tables 4, 5 and6. The nucleic acids of the present invention find use as primers andtemplates for the recombinant production of longevity-associatedpeptides or polypeptides, for chromosome and gene mapping, to provideantisense sequences, for tissue distribution studies, to locate andobtain full length genes, to identify and obtain homologous sequences(wild-type and mutants), and in diagnostic applications.

Antisense Oligonucleotides

In a particular embodiment of the invention, an antisense nucleic acidor oligonucleotide is wholly or partially complementary to, and canhybridize with, a target nucleic acid (either DNA or RNA) having thesequence of SEQ ID NO:1, NO:3 or any SEQ ID from Tables 2-7. Forexample, an antisense nucleic acid or oligonucleotide comprising 16nucleotides can be sufficient to inhibit expression of at least one genefrom Tables 4, 5 and 6. Alternatively, an antisense nucleic acid oroligonucleotide can be complementary to 5′ or 3′ untranslated regions,or can overlap the translation initiation codon (5′ untranslated andtranslated regions) of at least one gene from Tables 4, 5 and 6, or itsfunctional equivalent. In another embodiment, the antisense nucleic acidis wholly or partially complementary to, and can hybridize with, atarget nucleic acid that encodes a polypeptide from a gene described inTables 4, 5 and 6.

In addition, oligonucleotides can be constructed which will bind toduplex nucleic acid (i.e., DNA:DNA or DNA:RNA), to form a stable triplehelix containing or triplex nucleic acid. Such triplex oligonucleotidescan inhibit transcription and/or expression of a gene from Tables 4, 5and 6, or its functional equivalent (M. D. Frank-Kamenetskii et al.,1995). Triplex oligonucleotides are constructed using the base-pairingrules of triple helix formation and the nucleotide sequence of the genesdescribed in Tables 4, 5 and 6.

The present invention encompasses methods of using oligonucleotides inantisense inhibition of the function of the genes from Tables 4, 5 and6. In the context of this invention, the term “oligonucleotide” refersto naturally-occurring species or synthetic species formed fromnaturally-occurring subunits or their close homologs. The term may alsorefer to moieties that function similarly to oligonucleotides, but havenon-naturally-occurring portions. Thus, oligonucleotides may havealtered sugar moieties or inter-sugar linkages. Exemplary among theseare phosphorothioate and other sulfur containing species which are knownin the art. In preferred embodiments, at least one of the phosphodiesterbonds of the oligonucleotide has been substituted with a structure thatfunctions to enhance the ability of the compositions to penetrate intothe region of cells where the RNA whose activity is to be modulated islocated. It is preferred that such substitutions comprisephosphorothioate bonds, methyl phosphonate bonds, or short chain alkylor cycloalkyl structures. In accordance with other preferredembodiments, the phosphodiester bonds are substituted with structureswhich are, at once, substantially non-ionic and non-chiral, or withstructures which are chiral and enantiomerically specific. Persons ofordinary skill in the art will be able to select other linkages for usein the practice of the invention. Oligonucleotides may also includespecies that include at least some modified base forms. Thus, purinesand pyrimidines other than those normally found in nature may be soemployed. Similarly, modifications on the furanosyl portions of thenucleotide subunits may also be effected, as long as the essentialtenets of this invention are adhered to. Examples of such modificationsare 2′-O-alkyl- and 2′-halogen-substituted nucleotides. Somenon-limiting examples of modifications at the 2′ position of sugarmoieties which are useful in the present invention include OH, SH, SCH3,F, OCH3, OCN, O(CH2), NH2 and O(CH2)nCH3, where n is from 1 to about 10.Such oligonucleotides are functionally interchangeable with naturaloligonucleotides or synthesized oligonucleotides, which have one or moredifferences from the natural structure. All such analogs arecomprehended by this invention so long as they function effectively tohybridize with at least one gene from Tables 4, 5 and 6 DNA or RNA toinhibit the function thereof.

The oligonucleotides in accordance with this invention preferablycomprise from about 3 to about 50 subunits. It is more preferred thatsuch oligonucleotides and analogs comprise from about 8 to about 25subunits and still more preferred to have from about 12 to about 20subunits. As defined herein, a “subunit” is a base and sugar combinationsuitably bound to adjacent subunits through phosphodiester or otherbonds. Antisense nucleic acids or oligonucleotides can be produced bystandard techniques (see, e.g., Shewmaker et al., U.S. Pat. No.6,107,065). The oligonucleotides used in accordance with this inventionmay be conveniently and routinely made through the well-known techniqueof solid phase synthesis. Any other means for such synthesis may also beemployed; however, the actual synthesis of the oligonucleotides is wellwithin the abilities of the practitioner. It is also well known toprepare other oligonucleotide such as phosphorothioates and alkylatedderivatives.

The oligonucleotides of this invention are designed to be hybridizablewith RNA (e.g., mRNA) or DNA from genes described in Tables 4, 5 and 6.For example, an oligonucleotide (e.g., DNA oligonucleotide) thathybridizes to mRNA from a gene described in Tables 4, 5 and 6 can beused to target the mRNA for RnaseH digestion. Alternatively, anoligonucleotide that can hybridize to the translation initiation site ofthe mRNA of a gene described in Tables 4, 5 and 6 can be used to preventtranslation of the mRNA. In another approach, oligonucleotides that bindto the double-stranded DNA of a gene from Tables 4, 5 and 6 can beadministered. Such oligonucleotides can form a triplex construct andinhibit the transcription of the DNA encoding polypeptides of the genesdescribed in Tables 4, 5 and 6. Triple helix pairing prevents the doublehelix from opening sufficiently to allow the binding of polymerases,transcription factors, or regulatory molecules. Recent therapeuticadvances using triplex DNA have been described (see, e.g., J. E. Gee etal., 1994, Molecular and Immunologic Approaches, Futura Publishing Co.,Mt. Kisco, N.Y.).

As non-limiting examples, antisense oligonucleotides may be targeted tohybridize to the following regions: mRNA cap region; translationinitiation site; translational termination site; transcriptioninitiation site; transcription termination site; polyadenylation signal;3′ untranslated region; 5′ untranslated region; 5′coding region; midcoding region; and 3′ coding region. Preferably, the complementaryoligonucleotide is designed to hybridize to the most unique 5′ sequenceof a gene described in Tables 4, 5 and 6, including any of about 15-35nucleotides spanning the 5′ coding sequence. In accordance with thepresent invention, the antisense oligonucleotide can be synthesized,formulated as a pharmaceutical composition, and administered to asubject. The synthesis and utilization of antisense and triplexoligonucleotides have been previously described (e.g., Simon et al.,1999; Barre et al., 2000; Elez et al., 2000; Sauter et al., 2000).

Alternatively, expression vectors derived from retroviruses, adenovirus,herpes or vaccinia viruses or from various bacterial plasmids may beused for delivery of nucleotide sequences to the targeted organ, tissueor cell population. Methods which are well known to those skilled in theart can be used to construct recombinant vectors which will expressnucleic acid sequence that is complementary to the nucleic acid sequenceencoding a polypeptide from the genes described in Tables 4, 5 and 6.These techniques are described both in Sambrook et al., 1989 and inAusubel et al., 1992. For example, expression of at least one gene fromTables 4, 5 and 6 can be inhibited by transforming a cell or tissue withan expression vector that expresses high levels of untranslatable senseor antisense sequences. Even in the absence of integration into the DNA,such vectors may continue to transcribe RNA molecules until they aredisabled by endogenous nucleases. Transient expression may last for amonth or more with a nonreplicating vector, and even longer ifappropriate replication elements are included in the vector system.Various assays may be used to test the ability of gene-specificantisense oligonucleotides to inhibit the expression of at least onegene from Tables 4, 5 and 6. For example, mRNA levels of the genesdescribed in Tables 4, 5 and 6 can be assessed by Northern blot analysis(Sambrook et al., 1989; Ausubel et al., 1992; J. C. Alwine et al. 1977;I. M. Bird, 1998), quantitative or semi-quantitative RT-PCR analysis(see, e.g., W. M. Freeman et al., 1999; Ren et al., 1998; J. M. Cale etal., 1998), or in situ hybridization (reviewed by A. K. Raap, 1998).Alternatively, antisense oligonucleotides may be assessed by measuringlevels of the polypeptide from the genes described in Tables 4, 5 and 6,e.g., by western blot analysis, indirect immunofluorescence andimmunoprecipitation techniques (see, e.g., J. M. Walker, 1998, ProteinProtocols on CD-ROM, Humana Press, Totowa, N.J.). Any other means forsuch detection may also be employed, and is well within the abilities ofthe practitioner.

Mapping Technologies

The present invention includes various methods which employ mappingtechnologies to map SNPs and polymorphisms. For purpose of clarity, thissection comprises, but is not limited to, the description of mappingtechnologies that can be utilized to achieve the embodiments describedherein. Mapping technologies may be based on amplification methods,restriction enzyme cleavage methods, hybridization methods, sequencingmethods, and cleavage methods using agents.

Amplification methods include: self sustained sequence replication(Guatelli et al., 1990), transcriptional amplification system (Kwoh etal., 1989), Q-Beta Replicase (Lizardi et al., 1988), isothermalamplification (e.g. Dean et al., 2002; and Hafner et al., 2001), or anyother nucleic acid amplification method, followed by the detection ofthe amplified molecules using techniques well known to those of ordinaryskill in the art. These detection schemes are especially useful for thedetection of nucleic acid molecules if such molecules are present invery low number.

Restriction enzyme cleavage methods include: isolating sample andcontrol DNA, amplification (optional), digestion with one or morerestriction endonucleases, determination of fragment length sizes by gelelectrophoresis and comparing samples and controls. Differences infragment length sizes between sample and control DNA indicates mutationsin the sample DNA. Moreover, sequence specific ribozymes (see, e.g.,U.S. Pat. No. 5,498,531) or DNAzyme (e.g. U.S. Pat. No. 5,807,718) canbe used to score for the presence of specific mutations by developmentor loss of a ribozyme or DNAzyme cleavage site.

SNPs and SNP maps of the invention can be identified or generated byhybridizing sample nucleic acids, e.g., DNA or RNA, to high densityarrays or bead arrays containing oligonucleotide probes corresponding tothe SNPS of Tables 2, 3 and 7 (see the Affymetrix arrays and Illuminabead sets at www.affymetrix.com and www.illumina.com and see Cronin etal., 1996; or Kozal et al., 1996).

A variety of sequencing reactions known in the art can be used todirectly sequence nucleic acids for the presence or the absence of oneor more SNPs of Tables 2, 3 and 7. Examples of sequencing reactionsinclude those based on techniques developed by Maxam and Gilbert (1977)or Sanger (1977). It is also contemplated that any of a variety ofautomated sequencing procedures can be utilized, including sequencing bymass spectrometry (see, e.g. PCT International Publication No. WO94/16101; Cohen et al., 1996; and Griffin et al., 1993), real-timepyrophosphate sequencing method (Ronaghi et al., 1998; and Permutt etal., 2001) and sequencing by hybridization (see e.g. Drmanac et al.,2002).

Other methods of detecting SNPs include methods in which protection fromcleavage agents is used to detect mismatched bases in RNA/RNA, DNA/DNAor RNA/DNA heteroduplexes (Myers et al., 1985). In general, thetechnique of “mismatch cleavage” starts by providing heteroduplexesformed by hybridizing (labeled) RNA or DNA containing a wild-typesequence with potentially mutant RNA or DNA obtained from a sample. Thedouble-stranded duplexes are treated with an agent who cleavessingle-stranded regions of the duplex such as which will exist due tobasepair mismatches between the control and sample strands. Forinstance, RNA/DNA duplexes can be treated with RNase and DNA/DNA hybridstreated with S1 nuclease to enzymatically digest the mismatched regions.In other embodiments, either DNA/DNA or RNA/DNA duplexes can be treatedwith hydroxylamine or osmium tetroxide and with piperidine in order todigest mismatched regions. After digestion of the mismatched regions,the resulting material is then separated by size on denaturingpolyacrylamide gels to determine the site of a mutation or SNP. (see,for example, Cotton et al., 1988; and Saleeba et al., 1992). In apreferred embodiment, the control DNA or RNA can be labeled fordetection.

In still another embodiment, the mismatch cleavage reaction employs oneor more proteins that recognize mismatched base pairs in double-strandedDNA (so called “DNA mismatch repair” enzymes) in defined systems fordetecting and mapping SNPs. For example, the mutY enzyme of E. colicleaves A at G/A mismatches (Hsu et al., 1994). Other examples include,but are not limited to, the MutHLS enzyme complex of E. coli (Smith andModrich Proc. 1996) and Cel 1 from the celery (Kulinski et al., 2000)both cleave the DNA at various mismatches. According to an exemplaryembodiment, a probe based on a polymorphic site corresponding to a SNPof Tables 2, 3 and 7 is hybridized to a cDNA or other DNA product from atest cell or cells. The duplex is treated with a DNA mismatch repairenzyme, and the cleavage products, if any, can be detected fromelectrophoresis protocols or the like. See, for example, U.S. Pat. No.5,459,039. Alternatively, the screen can be performed in vivo followingthe insertion of the heteroduplexes in an appropriate vector. The wholeprocedure is known to those ordinary skilled in the art and is referredto as mismatch repair detection (see e.g. Fakhrai-Rad et al., 2004).

In other embodiments, alterations in electrophoretic mobility can beused to identify SNPs in a sample. For example, single strandconformation polymorphism (SSCP) can be used to detect differences inelectrophoretic mobility between mutant and wild type nucleic acids(Orita et al., 1989; Cotton et al., 1993; and Hayashi 1992).Single-stranded DNA fragments of case and control nucleic acids will bedenatured and allowed to renature. The secondary structure ofsingle-stranded nucleic acids varies according to sequence. Theresulting alteration in electrophoretic mobility enables the detectionof even a single base change. The DNA fragments may be labeled ordetected with labeled probes. The sensitivity of the assay may beenhanced by using RNA (rather than DNA), in which the secondarystructure is more sensitive to a change in sequence. In a preferredembodiment, the method utilizes heteroduplex analysis to separate doublestranded heteroduplex molecules on the basis of changes inelectrophoretic mobility (Kee et al., 1991).

In yet another embodiment, the movement of mutant or wild-type fragmentsin a polyacrylamide gel containing a gradient of denaturant is assayedusing denaturing gradient gel electrophoresis (DGGE) (Myers et al.,1985). When DGGE is used as the method of analysis, DNA will be modifiedto insure that it does not completely denature, for example by adding aGC clamp of approximately 40 bp of high-melting GC-rich DNA by PCR. In afurther embodiment, a temperature gradient is used in place of adenaturing gradient to identify differences in the mobility of controland sample DNA (Rosenbaum et al., 1987). In another embodiment, themutant fragment is detected using denaturing HPLC (see e.g. Hoogendoornet al., 2000).

Examples of other techniques for detecting SNPs include, but are notlimited to, selective oligonucleotide hybridization, selectiveamplification, selective primer extension, selective ligation,single-base extension, selective termination of extension or invasivecleavage assay. For example, oligonucleotide primers may be prepared inwhich the SNP is placed centrally and then hybridized to target DNAunder conditions which permit hybridization only if a perfect match isfound (Saiki et al., 1986; Saiki et al., 1989). Such oligonucleotidesare hybridized to PCR amplified target DNA or a number of differentmutations when the oligonucleotides are attached to the hybridizingmembrane and hybridized with labeled target DNA. Alternatively, theamplification, the allele-specific hybridization and the detection canbe done in a single assay following the principle of the 5′ nucleaseassay (e.g. see Livak et al., 1995). For example, the associated allele,a particular allele of a polymorphic locus, or the like is amplified byPCR in the presence of both allele-specific oligonucleotides, eachspecific for one or the other allele. Each probe has a differentfluorescent dye at the 5′ end and a quencher at the 3′ end. During PCR,if one or the other or both allele-specific oligonucleotides arehybridized to the template, the Taq polymerase via its 5′ exonucleaseactivity will release the corresponding dyes. The latter will thusreveal the genotype of the amplified product.

Hybridization assays may also be carried out with a temperature gradientfollowing the principle of dynamic allele-specific hybridization or likee.g. Jobs et al., (2003); and Bourgeois and Labuda, (2004). For example,the hybridization is done using one of the two allele-specificoligonucleotides labeled with a fluorescent dye, an intercalatingquencher under a gradually increasing temperature. At low temperature,the probe is hybridized to both the mismatched and full-matchedtemplate. The probe melts at a lower temperature when hybridized to thetemplate with a mismatch. The release of the probe is captured by anemission of the fluorescent dye, away from the quencher. The probe meltsat a higher temperature when hybridized to the template with nomismatch. The temperature-dependent fluorescence signals thereforeindicate the absence or presence of an associated allele, a particularallele of a polymorphic locus, or the like (e.g. Jobs et al., 2003).Alternatively, the hybridization is done under a gradually decreasingtemperature. In this case, both allele-specific oligonucleotides arehybridized to the template competitively. At high temperature none ofthe two probes are hybridized. Once the optimal temperature of thefull-matched probe is reached, it hybridizes and leaves no target forthe mismatched probe (e.g. Bourgeois and Labuda, 2004). In the lattercase, if the allele-specific probes are differently labeled, then theyare hybridized to a single PCR-amplified target. If the probes arelabeled with the same dye, then the probe cocktail is hybridized totwice to identical templates with only one labeled probes, different inthe two cocktails, in the presence of the unlabeled competitive probe.

Alternatively, allele specific amplification technology that depends onselective PCR amplification may be used in conjunction with the presentinvention. Oligonucleotides used as primers for specific amplificationmay carry the associated allele, a particular allele of a polymorphiclocus, or the like, also referred to as “mutation” of interest in thecenter of the molecule, so that amplification depends on differentialhybridization (Gibbs et al., 1989) or at the extreme 3′ end of oneprimer where, under appropriate conditions, mismatch can prevent, orreduce polymerase extension (Prossner, 1993). In addition it may bedesirable to introduce a novel restriction site in the region of themutation to create cleavage-based detection (Gasparini et al., 1992). Itis anticipated that in certain embodiments, amplification may also beperformed using Taq ligase for amplification (Barany, 1991). In suchcases, ligation will occur only if there is a perfect match at the 3′end of the 5′ sequence making it possible to detect the presence of aknown associated allele, a particular allele of a polymorphic locus, orthe like at a specific site by looking for the presence or absence ofamplification. The products of such an oligonucleotide ligation assaycan also be detected by means of gel electrophoresis. Furthermore, theoligonucleotides may contain universal tags used in PCR amplificationand zip code tags that are different for each allele. The zip code tagsare used to isolate a specific, labeled oligonucleotide that may containa mobility modifier (e.g. Grossman et al., 1994).

In yet another alternative, allele-specific elongation followed byligation will form a template for PCR amplification. In such cases,elongation will occur only if there is a perfect match at the 3′ end ofthe allele-specific oligonucleotide using a DNA polymerase. Thisreaction is performed directly on the genomic DNA and theextension/ligation products are amplified by PCR. To this end, theoligonucleotides contain universal tags allowing amplification at a highmultiplex level and a zip code for SNP identification. The PCR tags aredesigned in such a way that the two alleles of a SNP are amplified bydifferent forward primers, each having a different dye. The zip codetags are the same for both alleles of a given SNPs and they are used forhybridization of the PCR-amplified products to oligonucleotides bound toa solid support, chip, bead array or like. For an example of theprocedure, see Fan et al. (Cold Spring Harbor Symposia on QuantitativeBiology, Vol. LXVIII, pp. 69-78 2003).

Another alternative includes the single-base extension/ligation assayusing a molecular inversion probe, consisting of a single, longoligonucleotide (see e.g. Hardenbol et al., 2003). In such anembodiment, the oligonucleotide hybridizes on both side of the SNP locusdirectly on the genomic DNA, leaving a one-base gap at the SNP locus.The gap-filling, one-base extension/ligation is performed in four tubes,each having a different dNTP. Following this reaction, theoligonucleotide is circularized whereas unreactive, linearoligonucleotides are degraded using an exonuclease such as exonuclease Iof E. coli. The circular oligonucleotides are then linearized and theproducts are amplified and labeled using universal tags on theoligonucleotides. The original oligonucleotide also contains aSNP-specific zip code allowing hybridization to oligonucleotides boundto a solid support, chip, and bead array or like. This reaction can beperformed at a high multiplexed level.

In another alternative, the associated allele, a particular allele of apolymorphic locus, or the like is scored by single-base extension (seee.g. U.S. Pat. No. 5,888,819). The template is first amplified by PCR.The extension oligonucleotide is then hybridized next to the SNP locusand the extension reaction is performed using a thermostable polymerasesuch as ThermoSequenase (GE Healthcare) in the presence of labeledddNTPs. This reaction can therefore be cycled several times. Theidentity of the labeled ddNTP incorporated will reveal the genotype atthe SNP locus. The labeled products can be detected by means of gelelectrophoresis, fluorescence polarization (e.g. Chen et al., 1999) orby hybridization to oligonucleotides bound to a solid support, chip, andbead array or like. In the latter case, the extension oligonucleotidewill contain a SNP-specific zip code tag.

In yet another alternative, a SNP is scored by selective termination ofextension. The template is first amplified by PCR and the extensionoligonucleotide hybridizes in vicinity to the SNP locus, close to butnot necessarily adjacent to it. The extension reaction is carried outusing a thermostable polymerase such as Thermo Sequenase (GE Healthcare)in the presence of a mix of dNTPs and at least one ddNTP. The latter hasto terminate the extension at one of the allele of the interrogated SNP,but not both such that the two alleles will generate extension productsof different sizes. The extension product can then be detected by meansof gel electrophoresis, in which case the extension products need to belabeled, or by mass spectrometry (see e.g. Storm et al., 2003).

In another alternative, SNPs are detected using an invasive cleavageassay (see U.S. Pat. No. 6,090,543). There are five oligonucleotides perSNP to interrogate but these are used in a two step-reaction. During theprimary reaction, three of the designed oligonucleotides are firsthybridized directly to the genomic DNA. One of them is locus-specificand hybridizes up to the SNP locus (the pairing of the 3′ base at theSNP locus is not necessary). There are two allele-specificoligonucleotides that hybridize in tandem to the locus-specific probebut also contain a 5′ flap that is specific for each allele of the SNP.Depending upon hybridization of the allele-specific oligonucleotides atthe base of the SNP locus, this creates a structure that is recognizedby a cleavase enzyme (U.S. Pat. No. 6,090,606) and the allele-specificflap is released. During the secondary reaction, the flap fragmentshybridize to a specific cassette to recreate the same structure as aboveexcept that the cleavage will release a small DNA fragment labeled witha fluorescent dye that can be detected using regular fluorescencedetector. In the cassette, the emission of the dye is inhibited by aquencher.

Methods to Identify Agents that Modulate the Expression of a NucleicAcid Encoding a Gene Involved in Longevity.

The present invention provides methods for identifying agents thatmodulate the expression of a nucleic acid encoding a gene from Tables 4,5 and 6. Such methods may utilize any available means of monitoring forchanges in the expression level of the nucleic acids of the invention.As used herein, an agent is said to modulate the expression of a nucleicacid of the invention if it is capable of up- or down-regulatingexpression of the nucleic acid in a cell. Such cells can be obtainedfrom any parts of the body such as the scalp, blood, dermis, epidermisand other skin cells, cutaneous surfaces, intertrigious areas,genitalia, vessels and endothelium. Some non-limiting examples of cellsthat can be used are red blood cells, muscle cells, heart cells, nervecells, insulin-producing cells, pancreatic cells, brain cells, germcells, keratinocytes, monocytes, neutrophils, langerhans cells, CD4+ andCD8+ T cells, B and T lymphocytes, leukocytes, hormonal cells, bonemarrow cells, skin cells, buccal cells, spinal cord cells, bone cells,adipose cells, cartilage cells, dendritic cells, intestinal cells,hepatic cells, mucous cells, olfactory cells, retinal cells, somaticcells and arterial cells.

In one assay format, the expression of a nucleic acid encoding a gene ofthe invention (see Tables 4, 5 and 6) in a cell or tissue sample ismonitored directly by hybridization to the nucleic acids of theinvention. Cell lines or tissues are exposed to the agent to be testedunder appropriate conditions and time and total RNA or mRNA is isolatedby standard procedures such as those disclosed in Sambrook et al.,(1989) Molecular Cloning: A Laboratory Manual, Cold Spring HarborLaboratory Press).

Probes to detect differences in RNA expression levels between cellsexposed to the agent and control cells may be prepared as describedabove. Hybridization conditions are modified using known methods, suchas those described by Sambrook et al., and Ausubel et al., as requiredfor each probe. Hybridization of total cellular RNA or RNA enriched forpolyA RNA can be accomplished in any available format. For instance,total cellular RNA or RNA enriched for polyA RNA can be affixed to asolid support and the solid support exposed to at least one probecomprising at least one, or part of one of the sequences of theinvention under conditions in which the probe will specificallyhybridize. Alternatively, nucleic acid fragments comprising at leastone, or part of one of the sequences of the invention can be affixed toa solid support, such as a silicon chip or a porous glass wafer. Thechip or wafer can then be exposed to total cellular RNA or polyA RNAfrom a sample under conditions in which the affixed sequences willspecifically hybridize to the RNA. By examining for the ability of agiven probe to specifically hybridize to an RNA sample from an untreatedcell population and from a cell population exposed to the agent, agentswhich up or down regulate expression are identified.

Methods to Identify Agents that Modulate the Activity of a ProteinEncoded by a Gene Involved in Longevity.

The present invention provides methods for identifying agents thatmodulate at least one activity of the proteins described in Tables 4, 5and 6. Such methods may utilize any means of monitoring or detecting thedesired activity. As used herein, an agent is said to modulate theexpression of a protein of the invention if it is capable of up- ordown-regulating expression of the protein in a cell. Such cells can beobtained from any parts of the body such as the scalp, blood, dermis,epidermis and other skin cells, cutaneous surfaces, intertrigious areas,genitalia, vessels and endothelium. Some non-limiting examples of cellsthat can be used are red blood cells, muscle cells, heart cells, nervecells, insulin-producing cells, pancreatic cells, brain cells, germcells, keratinocytes, monocytes, neutrophils, langerhans cells, CD4+ andCD8+ T cells, B and T lymphocytes, leukocytes, hormonal cells, bonemarrow cells, skin cells, buccal cells, spinal cord cells, bone cells,adipose cells, cartilage cells, dendritic cells, intestinal cells,hepatic cells, mucous cells, olfactory cells, retinal cells, somaticcells and arterial cells.

In one format, the specific activity of a protein of the invention,normalized to a standard unit, may be assayed in a cell population thathas been exposed to the agent to be tested and compared to an unexposedcontrol cell population may be assayed. Cell lines or populations areexposed to the agent to be tested under appropriate conditions and time.Cellular lysates may be prepared from the exposed cell line orpopulation and a control, unexposed cell line or population. Thecellular lysates are then analyzed with the probe.

Antibody probes can be prepared by immunizing suitable mammalian hostsutilizing appropriate immunization protocols using the proteins of theinvention or antigen-containing fragments thereof. To enhanceimmunogenicity, these proteins or fragments can be conjugated tosuitable carriers. Methods for preparing immunogenic conjugates withcarriers such as BSA, KLH or other carrier proteins are well known inthe art. In some circumstances, direct conjugation using, for example,carbodiimide reagents may be effective; in other instances linkingreagents such as those supplied by Pierce Chemical Co. (Rockford, Ill.)may be desirable to provide accessibility to the hapten. The haptenpeptides can be extended at either the amino or carboxy terminus with acysteine residue or interspersed with cysteine residues, for example, tofacilitate linking to a carrier. Administration of the immunogens isconducted generally by injection over a suitable time period and withuse of suitable adjuvants, as is generally understood in the art. Duringthe immunization schedule, titers of antibodies are taken to determineadequacy of antibody formation. While the polyclonal antisera producedin this way may be satisfactory for some applications, forpharmaceutical compositions, use of monoclonal preparations ispreferred. Immortalized cell lines which secrete the desired monoclonalantibodies may be prepared using standard methods, see e.g., Kohler &Milstein (1992) or modifications which affect immortalization oflymphocytes or spleen cells, as is generally known. The immortalizedcell lines secreting the desired antibodies can be screened byimmunoassay in which the antigen is the peptide hapten, polypeptide orprotein. When the appropriate immortalized cell culture secreting thedesired antibody is identified, the cells can be cultured either invitro or by production in ascites fluid. The desired monoclonalantibodies may be recovered from the culture supernatant or from theascites supernatant. Fragments of the monoclonal antibodies or thepolyclonal antisera which contain the immunologically significantportion(s) can be used as antagonists, as well as the intact antibodies.Use of immunologically reactive fragments, such as Fab or Fab′fragments, is often preferable, especially in a therapeutic context, asthese fragments are generally less immunogenic than the wholeimmunoglobulin. The antibodies or fragments may also be produced, usingcurrent technology, by recombinant means. Antibody regions that bindspecifically to the desired regions of the protein can also be producedin the context of chimeras derived from multiple species. Antibodyregions that bind specifically to the desired regions of the protein canalso be produced in the context of chimeras from multiple species, forinstance, humanized antibodies. The antibody can therefore be ahumanized antibody or a human antibody, as described in U.S. Pat. No.5,585,089 or Riechmann et al. (1988).

Agents that are assayed in the above method can be randomly selected orrationally selected or designed. As used herein, an agent is said to berandomly selected when the agent is chosen randomly without consideringthe specific sequences involved in the association of the a protein ofthe invention alone or with its associated substrates, binding partners,etc. An example of randomly selected agents is the use of a chemicallibrary or a peptide combinatorial library, or a growth broth of anorganism. As used herein, an agent is said to be rationally selected ordesigned when the agent is chosen on a non-random basis which takes intoaccount the sequence of the target site or its conformation inconnection with the agent's action. Agents can be rationally selected orrationally designed by utilizing the peptide sequences that make upthese sites. For example, a rationally selected peptide agent can be apeptide whose amino acid sequence is identical to or a derivative of anyfunctional consensus site. The agents of the present invention can be,as examples, oligonucleotides, antisense polynucleotides, interferingRNA, peptides, peptide mimetics, antibodies, antibody fragments, smallmolecules, vitamin derivatives, as well as carbohydrates. Peptide agentsof the invention can be prepared using standard solid phase (or solutionphase) peptide synthesis methods, as is known in the art. In addition,the DNA encoding these peptides may be synthesized using commerciallyavailable oligonucleotide synthesis instrumentation and producedrecombinantly using standard recombinant production systems. Theproduction using solid phase peptide synthesis is necessitated ifnon-gene-encoded amino acids are to be included.

Another class of agents of the present invention includes antibodies orfragments thereof that bind to a protein encoded by a gene in Tables 4,5 and 6. Antibody agents can be obtained by immunization of suitablemammalian subjects with peptides, containing as antigenic regions, thoseportions of the protein intended to be targeted by the antibodies (seesection above of antibodies as probes for standard antibody preparationmethodologies).

In yet another class of agents, the present invention includes peptidemimetics that mimic the three-dimensional structure of the proteinencoded by a gene from Tables 4, 5 and 6. Such peptide mimetics may havesignificant advantages over naturally occurring peptides, including, forexample: more economical production, greater chemical stability,enhanced pharmacological properties (half-life, absorption, potency,efficacy, etc.), altered specificity (e.g., a broad-spectrum ofbiological activities), reduced antigenicity and others. In one form,mimetics are peptide-containing molecules that mimic elements of proteinsecondary structure. The underlying rationale behind the use of peptidemimetics is that the peptide backbone of proteins exists chiefly toorient amino acid side chains in such a way as to facilitate molecularinteractions, such as those of antibody and antigen. A peptide mimeticis expected to permit molecular interactions similar to the naturalmolecule. In another form, peptide analogs are commonly used in thepharmaceutical industry as non-peptide drugs with properties analogousto those of the template peptide. These types of non-peptide compoundsare also referred to as peptide mimetics or peptidomimetics (Fauchere,1986; Veber & Freidinger, 1985; Evans et al., 1987) which are usuallydeveloped with the aid of computerized molecular modeling. Peptidemimetics that are structurally similar to therapeutically usefulpeptides may be used to produce an equivalent therapeutic orprophylactic effect. Generally, peptide mimetics are structurallysimilar to a paradigm polypeptide (i.e., a polypeptide that has abiochemical property or pharmacological activity), but have one or morepeptide linkages optionally replaced by a linkage using methods known inthe art. Labeling of peptide mimetics usually involves covalentattachment of one or more labels, directly or through a spacer (e.g., anamide group), to non-interfering position(s) on the peptide mimetic thatare predicted by quantitative structure-activity data and molecularmodeling. Such non-interfering positions generally are positions that donot form direct contacts with the macromolecule(s) to which the peptidemimetic binds to produce the therapeutic effect. Derivitization (e.g.,labeling) of peptide mimetics should not substantially interfere withthe desired biological or pharmacological activity of the peptidemimetic. The use of peptide mimetics can be enhanced through the use ofcombinatorial chemistry to create drug libraries. The design of peptidemimetics can be aided by identifying amino acid mutations that increaseor decrease binding of the protein to its binding partners. Approachesthat can be used include the yeast two hybrid method (see Chien et al.,1991) and the phage display method. The two hybrid method detectsprotein-protein interactions in yeast (Fields et al., 1989). The phagedisplay method detects the interaction between an immobilized proteinand a protein that is expressed on the surface of phages such as lambdaand M13 (Amberg et al., 1993; Hogrefe et al., 1993). These methods allowpositive and negative selection for protein-protein interactions and theidentification of the sequences that determine these interactions.

Method to Diagnose Longevity Trait and Age-Related Disease

The present invention also relates to methods for diagnosing longevitytrait or a related disorder, preferably age-related diseases, adisposition to such trait, predisposition to such a trait and/ordisorder progression. In some methods, the steps comprise contacting atarget sample with (a) nucleic molecule(s) or fragments thereof andcomparing the concentration of individual mRNA(s) with the concentrationof the corresponding mRNA(s) from at least one healthy donor. Anaberrant (increased or decreased) mRNA level of at least one gene fromTables 4, 5 and 6, at least 5 or 10 genes from Tables 4, 5 and 6, atleast 20 genes from Tables 4, 5 and 6, at least 30 genes from Tables 4,5 and 6 determined in the sample in comparison to the control sample isan indication of longevity or a related disorder or a disposition tosuch kinds of disorders. For diagnosis, samples are from any parts ofthe body such as the scalp, blood, dermis, epidermis and other skincells, cutaneous surfaces, intertrigious areas, genitalia, vessels andendothelium. Some non-limiting examples of cells that can be used arered blood cells, muscle cells, heart cells, nerve cells,insulin-producing cells, pancreatic cells, brain cells, germ cells,keratinocytes, monocytes, neutrophils, langerhans cells, CD4+ and CD8+ Tcells, B and T lymphocytes, leukocytes, hormonal cells, bone marrowcells, skin cells, buccal cells, spinal cord cells, bone cells, adiposecells, cartilage cells, dendritic cells, intestinal cells, hepaticcells, mucous cells, olfactory cells, retinal cells, somatic cells andarterial cells.

For analysis of gene expression, total RNA is obtained from cellsaccording to standard procedures and, preferably, reverse-transcribed.Preferably, a DNAse treatment (in order to get rid of contaminatinggenomic DNA) is performed. Some non-limiting examples of cells that canbe used are: red blood cells, muscle cells, heart cells, nerve cells,insulin-producing cells, pancreatic cells, brain cells, germ cells,keratinocytes, monocytes, neutrophils, langerhans cells, CD4+ and CD8+ Tcells, B and T lymphocytes, leukocytes, hormonal cells, bone marrowcells, skin cells, buccal cells, spinal cord cells, bone cells, adiposecells, cartilage cells, dendritic cells, intestinal cells, hepaticcells, mucous cells, olfactory cells, retinal cells, somatic cells andarterial cells.

The nucleic acid molecule or fragment is typically a nucleic acid probefor hybridization or a primer for PCR. The person skilled in the art isin a position to design suitable nucleic acids probes based on theinformation provided in the Tables of the present invention. The targetcellular component, i.e. mRNA, e.g., in skin, may be detected directlyin situ, e.g. by in situ hybridization or it may be isolated from othercell components by common methods known to those skilled in the artbefore contacting with a probe. Detection methods include Northern blotanalysis, RNase protection, in situ methods, e.g. in situ hybridization,in vitro amplification methods (PCR, LCR, QRNA replicase orRNA-transcription/amplification (TAS, 3SR), reverse dot blot disclosedin EP-B10237362) and other detection assays that are known to thoseskilled in the art. Products obtained by in vitro amplification can bedetected according to established methods, e.g. by separating theproducts on agarose or polyacrylamide gels and by subsequent stainingwith ethidium bromide. Alternatively, the amplified products can bedetected by using labeled primers for amplification or labeled dNTPs.Preferably, detection is based on a microarray.

The probes (or primers) (or, alternatively, the reverse-transcribedsample mRNAs) can be detectably labeled, for example, with aradioisotope, a bioluminescent compound, a chemiluminescent compound, afluorescent compound, a metal chelate, or an enzyme.

The present invention also relates to the use of the nucleic acidmolecules or fragments described above for the preparation of adiagnostic composition for the diagnosis of longevity or a dispositionto such a trait.

The present invention also relates to the use of the nucleic acidmolecules of the present invention for the isolation or development of acompound which is useful for therapy of age-associated diseases. Forexample, the nucleic acid molecules of the invention and the dataobtained using said nucleic acid molecules for diagnosis of longevitytrait might allow for the identification of further genes which arespecifically dysregulated, and thus may be considered as potentialtargets for therapeutic interventions.

The invention further provides prognostic assays that can be used toidentify subjects having or at risk of developing age-associateddiseases. In such method, a test sample is obtained from a subject andthe amount and/or concentration of the nucleic acid described in Tables4, 5 and 6 is determined; wherein the presence of an associated allele,a particular allele of a polymorphic locus, or the likes in the nucleicacids sequences of this invention (see SEQ ID from Tables 2-7) can bediagnostic for a subject having or at risk of developing age-associateddiseases. As used herein, a “test sample” refers to a biological sampleobtained from a subject of interest. For example, a test sample can be abiological fluid, a cell sample, or tissue. A biological fluid can be,but is not limited to saliva, serum, mucus, urine, stools,spermatozoids, vaginal secretions, lymph, amiotic liquid, pleural liquidand tears. Some non-limiting examples of cells that can be used are redblood cells, muscle cells, heart cells, nerve cells, insulin-producingcells, pancreatic cells, brain cells, germ cells, keratinocytes,monocytes, neutrophils, langerhans cells, CD4+ and CD8+ T cells, B and Tlymphocytes, leukocytes, hormonal cells, bone marrow cells, skin cells,buccal cells, spinal cord cells, bone cells, adipose cells, cartilagecells, dendritic cells, intestinal cells, hepatic cells, mucous cells,olfactory cells, retinal cells, somatic cells and arterial cells.

Furthermore, the prognostic assays described herein can be used todetermine whether a subject can be administered an agent (e.g., anagonist, antagonist, peptidomimetic, polypeptide, nucleic acid such asantisense DNA or interfering RNA (RNAi), small molecule or other drugcandidate) to treat age-associated diseases. Specifically, these assayscan be used to predict whether an individual will have an efficaciousresponse or will experience adverse events in response to such an agent.For example, such methods can be used to determine whether a subject canbe effectively treated with an agent that modulates the expressionand/or activity of a gene from Tables 4, 5 and 6, or the nucleic acidsdescribed herein. In another example, an association study may beperformed to identify polymorphisms from Tables 2, 3 and 7 that areassociated with a given response to the agent e.g., an efficaciousresponse or the likelihood of one or more adverse events. Thus, thepresent invention provides methods for determining whether a subject canbe effectively treated with an agent for a disorder associated withaberrant expression or activity of a gene from Tables 4, 5 and 6 inwhich a test sample is obtained and nucleic acids or polypeptides fromTables 2, 3 or 7 are detected (e.g., wherein the presence of aparticular level of expression of a gene from Tables 4, 5 and 6 or aparticular allelic variant of such gene, such as polymorphism fromTables 2, 3 or 7, is diagnostic for a subject that can be administeredan agent to treat a trait or age-associated disease). In one embodiment,the method includes obtaining a sample from a subject suspected ofhaving age-associated diseases or an affected individual and exposingsuch sample to an agent. The expression and/or activity of the nucleicacids and or genes of the invention are monitored before and aftertreatment with such agent to assess the effect of such agent. Afteranalysis of the expression values, one skilled in the art can determinewhether such agent can effectively treat such subject. In anotherembodiment, the method includes obtaining a sample from a subject havingor susceptible to developing an age-associated disease and determiningthe allelic constitution of one or more polymorphism from Tables 2, 3 or7 that are associated with a particular response to an agent. Afteranalysis of the allelic constitution of the individual at the associatedpolymorphisms, one skilled in the art can determine whether such agentcan effectively treat such subject.

The methods of the invention can also be used to detect geneticalterations in a gene from Tables 4, 5 and 6, thereby determining if asubject with the lesioned gene is at risk for an age-associateddisorder. In preferred embodiments, the methods include detecting, in asample of cells from the subject, the presence or absence of a geneticalteration characterized by at least one alteration linked to oraffecting the integrity of a gene from Tables 4, 5 and 6 encoding apolypeptide or the misexpression of such gene. For example, such geneticalterations can be detected by ascertaining the existence of at leastone of: (1) a deletion of one or more nucleotides from a gene fromTables 4, 5 and 6; (2) an addition of one or more nucleotides to a genefrom Tables 4, 5 and 6; (3) a substitution of one or more nucleotides ofa gene from Tables 4, 5 and 6; (4) a chromosomal rearrangement of a genefrom Tables 4, 5 and 6; (5) an alteration in the level of a messengerRNA transcript of a gene from Tables 4, 5 and 6; (6) aberrantmodification of a gene from Tables 4, 5 and 6, such as of themethylation pattern of the genomic DNA, (7) the presence of a non-wildtype splicing pattern of a messenger RNA transcript of a gene fromTables 4, 5 and 6; (8) inappropriate post-translational modification ofa polypeptide encoded by a gene from Tables 4, 5 and 6; and (9)alternative promoter use. As described herein, there are a large numberof assay techniques known in the art which can be used for detectingalterations in a gene from Tables 4, 5 and 6. A preferred biologicalsample is a peripheral blood sample obtained by conventional means froma subject. Another preferred biological sample is a buccal swab. Otherbiological samples can be, but is not limited to, urine, stools,spermatozoids, vaginal secretions, lymph, amniotic liquid, pleuralliquid and tears. In certain embodiments, detection of the alterationinvolves the use of a probe/primer in a polymerase chain reaction (PCR)(see, e.g., U.S. Pat. Nos. 4,683,195 and 4,683,202), such as anchor PCRor RACE PCR, or alternatively, in a ligation chain reaction (LCR) (see,e.g., Landegran et al., 1988; and Nakazawa et al., 1994), the latter ofwhich can be particularly useful for detecting point mutations in a genefrom Tables 4, 5 and 6 (see Abavaya et al., 1995). This method caninclude the steps of collecting a sample of cells from a patient,isolating nucleic acid (e.g., genomic DNA, mRNA, or both) from the cellsof the sample, contacting the nucleic acid sample with one or moreprimers which specifically hybridize to a gene from Tables 4, 5 and 6under conditions such that hybridization and amplification of thenucleic acid from Tables 4, 5 and 6 (if present) occurs, and detectingthe presence or absence of an amplification product, or detecting thesize of the amplification product and comparing the length to a controlsample. PCR and/or LCR may be desirable to use as a preliminaryamplification step in conjunction with some of the techniques used fordetecting an associated allele, a particular allele of a polymorphiclocus, or the like described herein.

Alternative amplification methods include: self sustained sequencereplication (Guatelli et al., 1990), transcriptional amplificationsystem (Kwoh et al., 1989), Q-Beta Replicase (Lizardi et al., 1988),isothermal amplification (e.g. Dean et al., 2002); and Hafner et al.,2001), or any other nucleic acid amplification method, followed by thedetection of the amplified molecules using techniques well known tothose of ordinary skill in the art. These detection schemes areespecially useful for the detection of nucleic acid molecules if suchmolecules are present in very low number.

In an alternative embodiment, alterations in a gene from Tables 4, 5 and6, from a sample cell can be identified by identifying changes in arestriction enzyme cleavage pattern. For example, sample and control DNAis isolated, amplified (optionally), digested with one or morerestriction endonucleases, and fragment length sizes are determined bygel electrophoresis and compared. Differences in fragment length sizesbetween sample and control DNA indicates an associated allele, aparticular allele of a polymorphic locus, or the like, in the sampleDNA. Moreover, sequence specific ribozymes (see, e.g., U.S. Pat. No.5,498,531 or DNAzyme e.g. U.S. Pat. No. 5,807,718) can be used to scorefor the presence of specific associated allele, a particular allele of apolymorphic locus, or the likes by development or loss of a ribozyme orDNAzyme cleavage site.

The present invention also relates to further methods for diagnosing thelongevity trait or a related disorder, preferably an age-associateddisorder, a disposition to such disorder, and predisposition to such adisorder and/or disorder progression. In some methods, the stepscomprise contacting a target sample with (a) nucleic molecule(s) orfragments thereof and determining the presence or absence of aparticular allele of a polymorphism that confers a disorder-relatedphenotype (e.g., predisposition to such a disorder and/or disorderprogression). The presence of at least one allele from Tables 2, 3 or 7that is associated with the longevity trait (“associated allele”), atleast 5 or 10 associated alleles from Tables 2, 3 or 7, at least 50associated alleles from Tables 2, 3 or 7, at least 100 associatedalleles from Tables 2, 3 or 7, or at least 200 associated alleles fromTables 2, 3 or 7 determined in the sample is an indication of thelongevity trait or a related age-associated disorder, a disposition orpredisposition to such kinds of disorders, or a prognosis for suchdisorder progression. Samples may be obtained from any parts of the bodysuch as the scalp, blood, dermis, epidermis and other skin cells,cutaneous surfaces, intertrigious areas, genitalia, vessels andendothelium. Some non-limiting examples of cells that can be used arered blood cells, muscle cells, heart cells, nerve cells,insulin-producing cells, pancreatic cells, brain cells, germ cells,keratinocytes, monocytes, neutrophils, langerhans cells, CD4+ and CD8+ Tcells, lymphocytes, hormonal cells, bone marrow cells, skin cells,buccal cells, spinal cord cells, bone cells, adipose cells, cartilagecells, dendritic cells, intestinal cells, hepatic cells, mucous cells,olfactory cells, retinal cells, somatic cells and arterial cells.

In other embodiments, alterations in a gene from Tables 4, 5 and 6 or alocus from Tables 2, 3 or 7, or different alleles of the polymorphismsfrom Tables 2, 3 or 7 can be identified by hybridizing sample andcontrol nucleic acids, e.g., DNA or RNA, to high density arrays or beadarrays containing tens to thousands of oligonucleotide probes (Cronin etal., 1996; Kozal et al., 1996). For example, alterations in a gene fromTables 4, 5 and 6 or a locus from Tables 2, 3 or 7, or different allelesof the polymorphisms from Tables 2, 3 or 7 can be identified in twodimensional arrays containing light-generated DNA probes as described inCronin et al., (1996). Briefly, a first hybridization array of probescan be used to scan through long stretches of DNA in a sample andcontrol to identify base changes between the sequences by making lineararrays of sequential overlapping probes. This step allows theidentification of point mutations or different alleles of polymorphisms.This step is followed by a second hybridization array that allows thecharacterization of specific mutations, associated alleles or alleles ofa particular polymorphic locus, by using smaller, specialized probearrays complementary to all variants or mutations detected. Eachmutation array is composed of parallel probe sets, one complementary tothe wild-type gene and the other complementary to the mutant gene orassociated alleles or particular allele of a polymorphic locus.

In yet another embodiment, any of a variety of sequencing reactionsknown in the art can be used to directly sequence a gene from Tables 4,5 and 6 and detect an associated allele, a particular allele of apolymorphic locus, or the like by comparing the sequence of the samplegene from Tables 4, 5 and 6 with the corresponding wild-type (control)sequence. Examples of sequencing reactions include those based ontechniques developed by Maxam and Gilbert (1977) or Sanger (1977). It isalso contemplated that any of a variety of automated sequencingprocedures can be utilized when performing the diagnostic assays(Bio/Techniques 19:448, 1995) including sequencing by mass spectrometry(see, e.g. PCT International Publication No. WO 94/16101; Cohen et al.,1996; and Griffin et al. 1993), real-time pyrophosphate sequencingmethod (Ronaghi et al., 1998; and Permutt et al., 2001) and sequencingby hybridization (see e.g. Drmanac et al., 2002).

Other methods of detecting an associated allele, a particular allele ofa polymorphic locus, or the likes in a gene from Tables 4, 5 and 6include methods in which protection from cleavage agents is used todetect mismatched bases in RNA/RNA, DNA/DNA or RNA/DNA heteroduplexes(Myers et al., 1985). In general, the art technique of “mismatchcleavage” starts by providing heteroduplexes formed by hybridizing(labeled) RNA or DNA containing the wild-type gene from Tables 4, 5 and6 sequence with potentially mutant RNA or DNA obtained from a tissuesample. The double-stranded duplexes are treated with an agent thatcleaves single-stranded regions of the duplex such as which will existdue to basepair mismatches between the control and sample strands. Forinstance, RNA/DNA duplexes can be treated with RNase and DNA/DNA hybridstreated with S1 nuclease to enzymatically digest the mismatched regions.In other embodiments, either DNA/DNA or RNA/DNA duplexes can be treatedwith hydroxylamine or osmium tetroxide and with piperidine in order todigest mismatched regions. After digestion of the mismatched regions,the resulting material is then separated by size on denaturingpolyacrylamide gels to determine the site of an associated allele, aparticular allele of a polymorphic locus, or the like. (see, forexample, Cotton et al., 1988; Saleeba et al., 1992). In a preferredembodiment, the control DNA or RNA can be labeled for detection, asdescribed herein.

In still another embodiment, the mismatch cleavage reaction employs oneor more proteins that recognize mismatched base pairs in double-strandedDNA (so called “DNA mismatch repair” enzymes) in defined systems fordetecting and mapping point an associated allele, a particular allele ofa polymorphic locus, or the likes in a gene from Tables 4, 5 and 6 cDNAsobtained from samples of cells. For example, the mutY enzyme of E. colicleaves A at G/A mismatches (Hsu et al., 1994). Other examples include,but are not limited to, the MutHLS enzyme complex of E. coli (Smith andModrich., 1996) and Cel 1 from the celery (Kulinski et al., 2000) bothcleave the DNA at various mismatches. According to an exemplaryembodiment, a probe based on a gene sequence from Tables 4, 5 and 6 ishybridized to a cDNA or other DNA product from a test cell or cells. Theduplex is treated with a DNA mismatch repair enzyme, and the cleavageproducts, if any, can be detected using electrophoresis protocols or thelike. See, for example, U.S. Pat. No. 5,459,039. Alternatively, thescreen can be performed in vivo following the insertion of theheteroduplexes in an appropriate vector. The whole procedure is known tothose ordinary skilled in the art and is referred to as mismatch repairdetection (see e.g. Fakhrai-Rad et al., 2004).

In other embodiments, alterations in electrophoretic mobility can beused to identify an associated allele, a particular allele of apolymorphic locus, or the likes in genes from Tables 4, 5 and 6. Forexample, single strand conformation polymorphism (SSCP) can be used todetect differences in electrophoretic mobility between mutant and wildtype nucleic acids (Orita et al., 1993; see also Cotton, 1993; andHayashi et al., 1992). Single-stranded DNA fragments of sample andcontrol nucleic acids from Tables 4, 5 and 6 will be denatured andallowed to renature. The secondary structure of single-stranded nucleicacids varies according to sequence; the resulting alteration inelectrophoretic mobility enables the detection of even a single basechange. The DNA fragments may be labeled or detected with labeledprobes. The sensitivity of the assay may be enhanced by using RNA(rather than DNA), in which the secondary structure is more sensitive toa change in sequence. In a preferred embodiment, the method utilizesheteroduplex analysis to separate double stranded heteroduplex moleculeson the basis of changes in electrophoretic mobility (Kee et al., 1991).

In yet another embodiment, the movement of mutant or wild-type fragmentsin a polyacrylamide gel containing a gradient of denaturant is assayedusing denaturing gradient gel electrophoresis (DGGE) (Myers et al.,1985). When DGGE is used as the method of analysis, DNA will be modifiedto insure that it does not completely denature, for example by adding aGC clamp of approximately 40 bp of high-melting GC-rich DNA by PCR. In afurther embodiment, a temperature gradient is used in place of adenaturing gradient to identify differences in the mobility of controland sample DNA (Rosenbaum et al., 1987). In another embodiment, themutant fragment is detected using denaturing HPLC (see e.g. Hoogendoornet al., 2000).

Examples of other techniques for detecting point mutations, associatedalleles or alleles of a particular polymorphic locus include, but arenot limited to, selective oligonucleotide hybridization, selectiveamplification, selective primer extension, selective ligation,single-base extension, selective termination of extension or invasivecleavage assay. For example, oligonucleotide primers may be prepared inwhich the known associated allele, particular allele of a polymorphiclocus, or the like is placed centrally and then hybridized to target DNAunder conditions which permit hybridization only if a perfect match isfound (Saiki et al., 1986; Saiki et al., 1989). Such allele specificoligonucleotides are hybridized to PCR amplified target DNA or a numberof different associated allele, a particular allele of a polymorphiclocus, or the likes where the oligonucleotides are attached to thehybridizing membrane and hybridized with labeled target DNA.Alternatively, the amplification, the allele-specific hybridization andthe detection can be done in a single assay following the principle ofthe 5′ nuclease assay (e.g. see Livak et al., 1995). For example, theassociated allele, a particular allele of a polymorphic locus, or thelike locus is amplified by PCR in the presence of both allele-specificoligonucleotides, each specific for one or the other allele. Each probehas a different fluorescent dye at the 5′ end and a quencher at the 3′end. During PCR, if one or the other or both allele-specificoligonucleotides are hybridized to the template, the Taq polymerase viaits 5′ exonuclease activity will release the corresponding dyes. Thelatter will thus reveal the genotype of the amplified product.

The hybridization may also be carried out with a temperature gradientfollowing the principle of dynamic allele-specific hybridization or like(e.g. Jobs et al., 2003; and Bourgeois and Labuda, 2004). For example,the hybridization is done using one of the two allele-specificoligonucleotides labeled with a fluorescent dye, an intercalatingquencher under a gradually increasing temperature. At low temperature,the probe is hybridized to both the mismatched and full-matchedtemplate. The probe melts at a lower temperature when hybridized to thetemplate with a mismatch. The release of the probe is captured by anemission of the fluorescent dye, away from the quencher. The probe meltsat a higher temperature when hybridized to the template with nomismatch. The temperature-dependent fluorescence signals thereforeindicate the absence or presence of the associated allele, particularallele of a polymorphic locus, or the like (e.g. Jobs et al. supra).Alternatively, the hybridization is done under a gradually decreasingtemperature. In this case, both allele-specific oligonucleotides arehybridized to the template competitively. At high temperature none ofthe two probes is hybridized. Once the optimal temperature of thefull-matched probe is reached, it hybridizes and leaves no target forthe mismatched probe. In the latter case, if the allele-specific probesare differently labeled, then they are hybridized to a singlePCR-amplified target. If the probes are labeled with the same dye, thenthe probe cocktail is hybridizes twice to identical templates with onlyone labeled probes, different in the two cocktails, in the presence ofthe unlabeled competitive probe.

Alternatively, allele specific amplification technology that depends onselective PCR amplification may be used in conjunction with the presentinvention. Oligonucleotides used as primers for specific amplificationmay carry the associated allele, particular allele of a polymorphiclocus, or the like of interest in the center of the molecule, so thatamplification depends on differential hybridization (Gibbs et al., 1989)or at the extreme 3′ end of one primer where, under appropriateconditions, mismatch can prevent, or reduce polymerase extension(Prossner, 1993). In addition it may be desirable to introduce a novelrestriction site in the region of the associated allele, particularallele of a polymorphic locus, or the like to create cleavage-baseddetection (Gasparini et al., 1992). It is anticipated that in certainembodiments amplification may also be performed using Taq ligase foramplification (Barany, 1991). In such cases, ligation will occur only ifthere is a perfect match at the 3′ end of the 5′ sequence making itpossible to detect the presence of a known associated allele, aparticular allele of a polymorphic locus, or the like at a specific siteby looking for the presence or absence of amplification. The products ofsuch an oligonucleotide ligation assay can also be detected by means ofgel electrophoresis. Furthermore, the oligonucleotides may containuniversal tags used in PCR amplification and zip code tags that aredifferent for each allele. The zip code tags are used to isolate aspecific, labeled oligonucleotide that may contain a mobility modifier(e.g. Grossman et al., 1994).

In yet another alternative, allele-specific elongation followed byligation will form a template for PCR amplification. In such cases,elongation will occur only if there is a perfect match at the 3′ end ofthe allele-specific oligonucleotide using a DNA polymerase. Thisreaction is performed directly on the genomic DNA and theextension/ligation products are amplified by PCR. To this end, theoligonucleotides contain universal tags allowing amplification at a highmultiplex level and a zip code for SNP identification. The PCR tags aredesigned in such a way that the two alleles of a SNP are amplified bydifferent forward primers, each having a different dye. The zip codetags are the same for both alleles of a given SNP and they are used forhybridization of the PCR-amplified products to oligonucleotides bound toa solid support, chip, bead array or like. For an example of theprocedure, see Fan et al. (Cold Spring Harbor Symposia on QuantitativeBiology, Vol. LXVIII, pp. 69-78, 2003).

Another alternative includes the single-base extension/ligation assayusing a molecular inversion probe, consisting of a single, longoligonucleotide (see e.g. Hardenbol et al., 2003). In such anembodiment, the oligonucleotide hybridizes on both side of the SNP locusdirectly on the genomic DNA, leaving a one-base gap at the SNP locus.The gap-filling, one-base extension/ligation is performed in four tubes,each having a different dNTP. Following this reaction, theoligonucleotide is circularized whereas unreactive, linearoligonucleotides are degraded using an exonuclease such as exonuclease Iof E. coli. The circular oligonucleotides are then linearized and theproducts are amplified and labeled using universal tags on theoligonucleotides. The original oligonucleotide also contains aSNP-specific zip code allowing hybridization to oligonucleotides boundto a solid support, chip, bead array or like. This reaction can beperformed at a highly multiplexed level.

In another alternative, the associated allele, particular allele of apolymorphic locus, or the like is scored by single-base extension (seee.g. U.S. Pat. No. 5,888,819). The template is first amplified by PCR.The extension oligonucleotide is then hybridized next to the SNP locusand the extension reaction is performed using a thermostable polymerasesuch as ThermoSequenase (GE Healthcare) in the presence of labeledddNTPs. This reaction can therefore be cycled several times. Theidentity of the labeled ddNTP incorporated will reveal the genotype atthe SNP locus. The labeled products can be detected by means of gelelectrophoresis, fluorescence polarization (e.g. Chen et al., 1999) orby hybridization to oligonucleotides bound to a solid support, chip,bead array or like. In the latter case, the extension oligonucleotidewill contain a SNP-specific zip code tag.

In yet another alternative, the variant is scored by selectivetermination of extension. The template is first amplified by PCR and theextension oligonucleotide hybridizes in vicinity to the SNP locus, closeto but not necessarily adjacent to it. The extension reaction is carriedout using a thermostable polymerase such as Thermo Sequenase (GEHealthcare) in the presence of a mix of dNTPs and at least one ddNTP.The latter has to terminate the extension at one of the alleles of theinterrogated SNP, but not both such that the two alleles will generateextension products of different sizes. The extension product can then bedetected by means of gel electrophoresis, in which case the extensionproducts need to be labeled, or by mass spectrometry (see e.g. Storm etal., 2003).

In another alternative, the associated allele, particular allele of apolymorphic locus, or the like is detected using an invasive cleavageassay (see U.S. Pat. No. 6,090,543). There are five oligonucleotides perSNP to interrogate but these are used in a two step-reaction. During theprimary reaction, three of the designed oligonucleotides are firsthybridized directly to the genomic DNA. One of them is locus-specificand hybridizes up to the SNP locus (the pairing of the 3′ base at theSNP locus is not necessary). There are two allele-specificoligonucleotides that hybridize in tandem to the locus-specific probebut also contain a 5′ flap that is specific for each allele of the SNP.Depending upon hybridization of the allele-specific oligonucleotides atthe base of the SNP locus, this creates a structure that is recognizedby a cleavase enzyme (U.S. Pat. No. 6,090,606) and the allele-specificflap is released. During the secondary reaction, the flap fragmentshybridize to a specific cassette to recreate the same structure as aboveexcept that the cleavage will release a small DNA fragment labeled witha fluorescent dye that can be detected using regular fluorescencedetector. In the cassette, the emission of the dye is inhibited by aquencher.

Other types of markers can also be used for diagnostic purposes. Forexample, microsatellites can also be useful to detect the geneticpredisposition of an individual to a given disorder. Microsatellitesconsist of short sequence motifs of one or a few nucleotides repeated intandem. The most common motifs are polynucleotide runs, dinucleotiderepeats (particularly the CA repeats) and trinucleotide repeats.However, other types of repeats can also be used. The microsatellitesare very useful for genetic mapping because they are highly polymorphicin their length. Microsatellite markers can be typed by various means,including but not limited to DNA PCR fragment sizing, oligonucleotideligation assay and mass spectrometry. For example, the locus of themicrosatellite is amplified by PCR and the size of the PCR fragment willbe directly correlated to the length of the microsatellite repeat. Thesize of the PCR fragment can be detected by regular means of gelelectrophoresis. The fragment can be labeled internally during PCR or byusing end-labeled oligonucleotides in the PCR reaction (e.g. Mansfieldet al., 1996). Alternatively, the size of the PCR fragment is determinedby mass spectrometry. In such a case, however, the flanking sequencesneed to be eliminated. This can be achieved by ribozyme cleavage of anRNA transcript of the microsatellite repeat (Krebs et al., 2001). Forexample, the microsatellite locus is amplified using oligonucleotidesthat include a T7 promoter on one end and a ribozyme motif on the otherend. Transcription of the amplified fragments will yield an RNAsubstrate for the ribozyme, releasing small RNA fragments that containthe repeated region. The size of the latter is determined by massspectrometry. Alternatively, the flanking sequences are specificallydegraded. This is achieved by replacing the dTTP in the PCR reaction bydUTP. The dUTP nucleosides are then removed by uracyl DNA glycosylasesand the resulting abasic sites are cleaved by either abasicendonucleases such as human AP endonuclease or chemical agents such aspiperidine. Bases can also be modified post-PCR by chemical agents suchas dimethyl sulfate and then cleaved by other chemical agents such aspiperidine (see e.g. Maxam and Gilbert, 1977; U.S. Pat. No. 5,869,242;and U.S. Patent pending Ser. No. 60/335,068).

In another alternative, an oligonucleotide ligation assay can beperformed. The microsatellite locus is first amplified by PCR. Then,different oligonucleotides can be submitted to ligation at the center ofthe repeat with a set of oligonucleotides covering all the possiblelengths of the marker at a given locus (Zirvi et al., 1999). Anotherexample of design of an oligonucleotide assay comprises the ligation ofthree oligonucleotides; a 5′ oligonucleotide hybridizing to the 5′flanking sequence, a repeat oligonucleotide of the length of theshortest allele of the marker hybridizing to the repeated region and aset of 3′ oligonucleotides covering all the existing alleles hybridizingto the 3′ flanking sequence and a portion of the repeated region for allthe alleles longer than the shortest one. For the shortest allele, the3′ oligonucleotide exclusively hybridizes to the 3′ flanking sequence(U.S. Pat. No. 6,479,244).

The methods described herein may be performed, for example, by utilizingpre-packaged diagnostic kits comprising at least one probe nucleic acidselected from the SEQ ID of Tables 2-7, or antibody reagent describedherein, which may be conveniently used, for example, in a clinicalsetting to diagnose patient exhibiting symptoms or a family history of adisorder or genetic trait, or disorder involving abnormal activity ofgenes from Tables 4, 5 and 6.

Method to Treat an Animal Suspected of Having Age-Associated Diseases

The present invention provides methods of treating a disorder associatedwith the longevity trait, such age-associated diseases by expressing invivo the nucleic acids of at least one gene from Tables 4, 5 and 6.These nucleic acids can be inserted into any of a number of well-knownvectors for the transfection of target cells and organisms as describedbelow. The nucleic acids are transfected into cells, ex vivo or in vivo,through the interaction of the vector and the target cell. The nucleicacids encoding a gene from Tables 4, 5 and 6, under the control of apromoter, then expresses the encoded protein, thereby mitigating theeffects of absent, partial inactivation, or abnormal expression of agene from Tables 4, 5 and 6.

Such gene therapy procedures have been used to correct acquired andinherited genetic defects, cancer, and viral infection in a number ofcontexts. The ability to express artificial genes in humans facilitatesthe prevention and/or cure of many important human disorders, includingmany disorders which are not amenable to treatment by other therapies(for a review of gene therapy procedures, see Anderson, 1992; Nabel &Felgner, 1993; Mitani & Caskey, 1993; Mulligan, 1993; Dillon, 1993;Miller, 1992; Van Brunt, 1998; Vigne, 1995; Kremer & Perricaudet 1995;Doerfler & Bohm 1995; and Yu et al., 1994).

Delivery of the gene or genetic material into the cell is the firstcritical step in gene therapy treatment of disorder or genetic traits. Alarge number of delivery methods are well known to those of skill in theart. Preferably, the nucleic acids are administered for in vivo or exvivo gene therapy uses. Non-viral vector delivery systems include DNAplasmids, naked nucleic acid, and nucleic acid complexed with a deliveryvehicle such as a liposome. Viral vector delivery systems include DNAand RNA viruses, which have either episomal or integrated genomes afterdelivery to the cell. For a review of gene therapy procedures, see thereferences included in the above section.

The use of RNA or DNA viral based systems for the delivery of nucleicacids take advantage of highly evolved processes for targeting a virusto specific cells in the body and trafficking the viral payload to thenucleus. Viral vectors can be administered directly to patients (invivo) or they can be used to treat cells in vitro and the modified cellsare administered to patients (ex vivo). Conventional viral based systemsfor the delivery of nucleic acids could include retroviral, lentivirus,adenoviral, adeno-associated and herpes simplex virus vectors for genetransfer. Viral vectors are currently the most efficient and versatilemethod of gene transfer in target cells and tissues. Integration in thehost genome is possible with the retrovirus, lentivirus, andadeno-associated virus gene transfer methods, often resulting in longterm expression of the inserted transgene. Additionally, hightransduction efficiencies have been observed in many different celltypes and target tissues.

The tropism of a retrovirus can be altered by incorporating foreignenvelope proteins, expanding the potential target population of targetcells. Lentiviral vectors are retroviral vector that are able totransduce or infect non-dividing cells and typically produce high viraltiters. Selection of a retroviral gene transfer system would thereforedepend on the target tissue. Retroviral vectors are comprised ofcis-acting long terminal repeats with packaging capacity for up to 6-10kb of foreign sequence. The minimum cis-acting LTRs are sufficient forreplication and packaging of the vectors, which are then used tointegrate the therapeutic gene into the target cell to provide permanenttransgene expression. Widely used retroviral vectors include those basedupon murine leukemia virus (MuLV), gibbon ape leukemia virus (GaLV),Simian Immuno deficiency virus (SIV), human immuno deficiency virus(HIV), and combinations thereof (see, e.g., Buchscher et al., 1992;Johann et al., 1992; Sommerfelt et al., 1990; Wilson et al., 1989;Miller et al., 1999; and PCT/US94/05700).

In applications where transient expression of the nucleic acid ispreferred, adenoviral based systems are typically used. Adenoviral basedvectors are capable of very high transduction efficiency in many celltypes and do not require cell division. With such vectors, high titerand levels of expression have been obtained. This vector can be producedin large quantities in a relatively simple system. Adeno-associatedvirus (“AAV”) vectors are also used to transduce cells with targetnucleic acids, e.g., in the in vitro production of nucleic acids andpeptides, and for in vivo and ex vivo gene therapy procedures (see,e.g., West et al., 1987; U.S. Pat. No. 4,797,368; WO 93/24641; Kotin,1994; Muzyczka, 1994). Construction of recombinant AAV vectors aredescribed in a number of publications, including U.S. Pat. No.5,173,414; Tratschin et al., 1985; Tratschin, et al., 1984; Hermonat &Muzyczka, 1984; and Samulski et al., 1989.

In particular, numerous viral vector approaches are currently availablefor gene transfer in clinical trials, with retroviral vectors by far themost frequently used system. All of these viral vectors utilizeapproaches that involve complementation of defective vectors by genesinserted into helper cell lines to generate the transducing agent. pLASNand MFG-S are examples are retroviral vectors that have been used inclinical trials (Dunbar et al., 1995; Kohn et al., 1995; Malech et al.,1997). PA317/pLASN was the first therapeutic vector used in a genetherapy trial (Blaese et al., 1995). Transduction efficiencies of 50% orgreater have been observed for MFG-S packaged vectors (Ellem et al.,1997; and Dranoff et al., 1997).

Recombinant adeno-associated virus vectors (rAAV) are a promisingalternative gene delivery systems based on the defective andnonpathogenic parvovirus adeno-associated type 2 virus. All vectors arederived from a plasmid that retains only the AAV 145 bp invertedterminal repeats flanking the transgene expression cassette. Efficientgene transfer and stable transgene delivery due to integration into thegenomes of the transduced cell are key features for this vector system.(Wagner et al., 1998, Kearns et al 1996).

Replication-deficient recombinant adenoviral vectors (Ad) arepredominantly used in transient expression gene therapy; because theycan be produced at high titer and they readily infect a number ofdifferent cell types. Most adenovirus vectors are engineered such that atransgene replaces the Ad E1a, E1b, and E3 genes; subsequently thereplication defector vector is propagated in human 293 cells that supplydeleted gene function in trans. Ad vectors can transduce multiple typesof tissues in vivo, including nondividing, differentiated cells such asthose found in the liver, kidney and muscle system tissues. ConventionalAd vectors have a large carrying capacity. An example of the use of anAd vector in a clinical trial involved polynucleotide therapy forantitumor immunization with intramuscular injection (Sterman et al.,1998). Additional examples of the use of adenovirus vectors for genetransfer in clinical trials include Rosenecker et al., 1996; Sterman etal., 1998; Welsh et al., 1995; Alvarez et al., 1997; Topf et al., 1998.

Packaging cells are used to form virus particles that are capable ofinfecting a host cell. Such cells include 293 cells, which packageadenovirus, and ψ2 cells or PA317 cells, which package retrovirus. Viralvectors used in gene therapy are usually generated by producer cell linethat packages a nucleic acid vector into a viral particle. The vectorstypically contain the minimal viral sequences required for packaging andsubsequent integration into a host, other viral sequences being replacedby an expression cassette for the protein to be expressed. The missingviral functions are supplied in trans by the packaging cell line. Forexample, AAV vectors used in gene therapy typically only possess ITRsequences from the AAV genome which are required for packaging andintegration into the host genome. Viral DNA is packaged in a cell line,which contains a helper plasmid encoding the other AAV genes, namely repand cap, but lacking ITR sequences. The cell line is also infected withadenovirus as a helper. The helper virus promotes replication of the AAVvector and expression of AAV genes from the helper plasmid. The helperplasmid is not packaged in significant amounts due to a lack of ITRsequences. Contamination with adenovirus can be reduced by, e.g., heattreatment to which adenovirus is more sensitive than AAV.

In many gene therapy applications, it is desirable that the gene therapyvector be delivered with a high degree of specificity to a particulartissue type. A viral vector is typically modified to have specificityfor a given cell type by expressing a ligand as a fusion protein with aviral coat protein on the viruses outer surface. The ligand is chosen tohave affinity for a receptor known to be present on the cell type ofinterest. For example, Han et al., 1995, reported that Moloney murineleukemia virus can be modified to express human heregulin fused to gp70,and the recombinant virus infects certain human breast cancer cellsexpressing human epidermal growth factor receptor. This principle can beextended to other pairs of virus expressing a ligand fusion protein andtarget cell expressing a receptor. For example, filamentous phage can beengineered to display antibody fragments (e.g., Fab or Fv) havingspecific binding affinity for virtually any chosen cellular receptor.Although the above description applies primarily to viral vectors, thesame principles can be applied to nonviral vectors. Such vectors can beengineered to contain specific uptake sequences thought to favor uptakeby specific target cells.

Gene therapy vectors can be delivered in vivo by administration to anindividual patient, typically by systemic administration (e.g.,intravenous, intraperitoneal, intramuscular, subdermal, or intracranialinfusion) or topical application. Alternatively, vectors can bedelivered to cells ex vivo, such as cells explanted from an individualpatient (e.g., lymphocytes, bone marrow aspirates, and tissue biopsy) oruniversal donor hematopoietic stem cells, followed by reimplantation ofthe cells into a patient, usually after selection for cells which haveincorporated the vector.

Ex vivo cell transfection for diagnostics, research, or for gene therapy(e.g., via re-infusion of the transfected cells into the host organism)is well known to those of skill in the art. In a preferred embodiment,cells are isolated from the subject organism, transfected with a nucleicacid (gene or cDNA), and re-infused back into the subject organism(e.g., patient). Various cell types suitable for ex vivo transfectionare well known to those of skill in the art (see, e.g., Freshney et al.,1994; and the references cited therein for a discussion of how toisolate and culture cells from patients).

In one embodiment, stem cells are used in ex vivo procedures for celltransfection and gene therapy. The advantage to using stem cells is thatthey can be differentiated into other cell types in vitro, or can beintroduced into a mammal (such as the donor of the cells) where theywill engraft in the bone marrow. Methods for differentiating CD34+ cellsin vitro into clinically important immune cell types using cytokinessuch a GM-CSF, IFN-γ and TNF-α are known (see Inaba et al., 1992).

Stem cells are isolated for transduction and differentiation using knownmethods. For example, stem cells are isolated from bone marrow cells bypanning the bone marrow cells with antibodies which bind unwanted cells,such as CD4+ and CD8+ (T cells), CD45+(panB cells), GR-1 (granulocytes),and lad (differentiated antigen presenting cells).

Vectors (e.g., retroviruses, adenoviruses, liposomes, etc.) containingtherapeutic nucleic acids can be also administered directly to theorganism for transduction of cells in vivo. Alternatively, naked DNA canbe administered.

Administration is by any of the routes normally used for introducing amolecule into ultimate contact with blood or tissue cells, as describedabove. The nucleic acids from Tables 2-7 are administered in anysuitable manner, preferably with the pharmaceutically acceptablecarriers described above. Suitable methods of administering such nucleicacids are available and well known to those of skill in the art, and,although more than one route can be used to administer a particularcomposition, a particular route can often provide a more immediate andmore effective reaction than another route (see Samulski et al., 1989).The present invention is not limited to any method of administering suchnucleic acids, but preferentially uses the methods described herein.

The present invention further provides other methods of treatingdisorders, such as age-associated disorders, by for exampleadministering to an individual having an age-associated disorder (orsuspected of having a age-associated disorder) an effective amount of anagent that regulates the expression, activity or physical state of atleast one gene from Tables 4, 5 and 6. An “effective amount” of an agentis an amount that modulates a level of expression or activity of a genefrom Tables 4, 5 and 6, in a cell in the individual at least about 10%,at least about 20%, at least about 30%, at least about 40%, at leastabout 50%, at least about 60%, at least about 70%, at least about 80% ormore, compared to a level of the respective gene from Tables 4, 5 and 6in a cell in the individual in the absence of the compound. Thepreventive or therapeutic agents of the present invention may beadministered, either orally or parenterally, systemically or locally.For example, intravenous injection such as drip infusion, intramuscularinjection, intraperitoneal injection, subcutaneous injection,suppositories, intestinal lavage, oral enteric coated tablets, and thelike can be selected, and the method of administration may be chosen, asappropriate, depending on the age and the conditions of the patient. Theeffective dosage is chosen from the range of 0.01 mg to 100 mg per kg ofbody weight per administration. Alternatively, the dosage in the rangeof 1 to 1000 mg, preferably 5 to 50 mg per patient may be chosen. Thetherapeutic efficacy of the treatment may be monitored by observingvarious parts of the body and mind, such as the response to a cognitivetest, by any monitoring method known in the art, such as the MMSE(mini-mental state examination). Others ways of monitoring efficacy canbe, but are not limited to monitoring well-being, memory, mental stateand psychological state of the patient.

The present invention further provides a method of treating anindividual clinically diagnosed with a trait or age-associated disorder.The methods generally comprises analyzing a biological sample thatincludes a cell, in some cases, a skin cell, from an individualclinically diagnosed with an age-associated disorder for the presence ofmodified levels of expression of at least 1 gene, at least 10 genes, atleast 30 genes from Tables 4, 5 and 6. A treatment plan that is mosteffective for individuals clinically diagnosed as having a conditionassociated with a trait or age-associated disorder is then selected onthe basis of the detected expression of such genes in a cell. Treatmentmay include administering a composition that includes an agent thatmodulates the expression or activity of a protein from Tables 4, 5 and 6in the cell. Information obtained as described in the methods above canalso be used to predict the response of the individual to a particularagent. Thus, the invention further provides a method for predicting apatient's likelihood to respond to a drug treatment for a conditionassociated with the longevity trait, comprising determining whethermodified levels of a gene from Tables 4, 5 and 6 is present in a cell,wherein the presence of protein is predictive of the patient'slikelihood to respond to a drug treatment for the condition. Examples ofthe prevention or improvement of symptoms accompanied by age-associateddisorders that can monitor for effectiveness include response to acognitive test, by any monitoring method known in the art, such as theMMSE (mini-mental state examination). Others ways of monitoring efficacycan be, but are not limited to monitoring well-being, memory, mentalstate and psychological state of the patient.

The invention also provides a method of predicting a response to therapyin a subject having age-associated disorders by determining the presenceor absence in the subject of one or more markers associated with thelongevity trait described in Tables 2, 3 and/or 7, diagnosing thesubject in which the one or more markers are present as having anage-associated disorder, and predicting a response to a therapy based onthe diagnosis e.g., response to therapy may include an efficaciousresponse and/or one or more adverse events. The invention also providesa method of optimizing therapy in a subject having an age-associateddisorder by determining the presence or absence in the subject of one ormore markers associated with a clinical subtype of age-associateddisorders, diagnosing the subject in which the one or more markers arepresent as having a particular clinical subtype of age-associateddisorders, and treating the subject having a particular clinical subtypeof age-associated disorders based on the diagnosis.

Thus, while there are a number of treatments for age-associateddisorders currently available, they all are accompanied by various sideeffects, high costs, and long complicated treatment protocols, which areoften not available and effective in a large number of individuals.Accordingly, there remains a need in the art for more effective andotherwise improved methods for treating and preventing age-associateddisorders. Thus, there is a continuing need in the medical arts forgenetic markers of longevity trait and guidance for the use of suchmarkers. The present invention fulfills this need and provides furtherrelated advantages.

EXAMPLES Example 1 Identification of Cases and Controls

All individuals were sampled from the Quebec founder population (QFP).Membership in the founder population was defined as having fourgrandparents with French Canadian family names who were born in theProvince of Quebec, Canada or in adjacent areas of the Provinces of NewBrunswick and Ontario or in New England or New York State. The Quebecfounder population has two distinct advantages over general populationsfor LD mapping. Because it is relatively young, about 12 to 15generations from mid-17th century to present, and because it has alimited but sufficient number of founders, approximately 2600 effectivefounders (Charbonneau et al. 1987), the Quebec population ischaracterized both by extended LD and by decreased geneticheterogeneity. The increased extent of LD allows the detection of genesaffecting the trait using a reasonable marker density, while stillallowing the increased meiotic resolution of population-based mapping.The number of founders is small enough to result in increased LD andreduced allelic heterogeneity, yet large enough to insure that all ofthe major genes affecting the trait involved in general populations arepresent in Quebec. Reduced allelic heterogeneity will act to increaserelative risk imparted by the remaining alleles and so increase thepower of case/control studies to detect genes and trait associatedalleles within the Quebec population. The specific combination of age ingenerations, optimal number of founders and large present populationsize makes the QFP optimal for LD-based gene mapping. The familyrelationships among samples are routinely examined using proprietaryalgorithms and information from the genealogical data bases. When twosubjects are found to be too closely related for LD analysis, one ofthem is removed from the sample.

Case inclusion criteria for the study included being 94 years of age orolder. Control inclusion criterion for the study included being 65 yearsof age or younger and gender matched to cases.

All human sampling was subject to ethical review procedures.

All enrolled QFP subjects (cases and controls) provided a 30 ml bloodsample (3 barcoded tubes of 10 ml). Samples were processed immediatelyupon arrival at Genizon's laboratory. All samples were scanned andlogged into a LabVantage Laboratory Information Management System(LIMS), which served as a hub between the clinical data managementsystem and the genetic analysis system. Following centrifugation, thebuffy coat containing the white blood cells was isolated from each tube.Genomic DNA was extracted from the buffy coat from one of the tubes, andstored at 4° C. until required for genotyping. DNA extraction wasperformed with a commercial kit using a guanidine hydrochloride basedmethod (FlexiGene, Qiagen) according to the manufacturer's instructions.The extraction method yielded high molecular weight DNA, and the qualityof every DNA sample was verified by agarose gel electrophoresis. GenomicDNA appeared on the gel as a large band of very high molecular weight.The remaining two buffy coats were stored at −80° C. as backups.

The samples were collected as 615 cases and 615 controls (127 males and488 females, for both cases and controls). The DNA extracted from casesand control samples was pooled together in various case and controlpools.

Separate case and control and male and female pools were constructed.The probands are also segregated according to their age at the time ofrecruitment and the proband females are further separated in two groups,those who failed a cognitive test and those who passed the test. Twoproband male pools contain 53-74 individuals, separated by age group.One proband female pool contains the 71 females who failed a cognitivetest whereas the 7 remaining pools consisted of 43-80 proband femaleswho passed the test, separated by age group. Ten (10) control poolsconsisted of 8 pools of 61 female samples and 2 pools of 63-64 malesamples.

Example 2 Genome Wide Association

Genotyping was performed using Perlegen Life Sciencesultra-high-throughput platform. Loci of interest were amplified andhybridized to wafers containing arrays of oligonucleotides. Allelediscrimination was performed through allele-specific hybridization. Intotal, 248,535 SNPs, spread over 3 microarrays, were genotyped. This setof markers contained the QLDM (Quebec LD Map), a map createdspecifically for the Quebec founder population, which possesses a basedensity of one marker per 40 kb and up to one marker per 10 kb in low-LDregions, the lower the LD is in a given area, the higher the markerdensity will be. The QLDM markers and other markers were selected fromvarious databases including the ˜1.6 million SNP database of PerlegenLife Sciences (Patil, 2001), the hapmap consortium database and dbSNP atNCBI. The SNPs were chosen to maximize uniformity of genetic coverageand as much as possible, with a minor allele frequency of 10% or higher.

The genotyping information was entered into a Unified Genotype Database(a proprietary database under development) from which it was accessedusing custom-built programs for export to the genetic analysis pipeline.Analyses of these genotypes were performed with the statistical toolsdescribed in Example 3. The GWS permitted the identification of 47candidate regions that are further analyzed by the Confirmation Mappingand Fine Mapping approaches described below.

Example 3 Genetic Analysis

The raw data generated by the GWS approach (Example 2 herein) wasanalyzed by various means to identify candidate regions (see alsoConfirmatory Mapping and Fine Mapping described in Example 5).

Raw Data Analysis by Perlegen

The data analysis process compares the relative fluorescence intensitiesof features corresponding to the reference allele of a given SNP withthose corresponding to the alternate allele, to calculate a p-hat value.The latter is proportional to the fluorescence signal from perfect matchfeatures for the reference allele divided by the sum of fluorescencesignals from perfect match features for the reference plus the alternatealleles. P-hat assumes values close to 1 (typically 0.9) for purereference samples and close to 0 (typically 0.1) for pure alternatesamples, and can be used as a measured estimate of the reference allelefrequency of a SNP in a DNA pool. The difference between case andcontrol pools, delta p-hat, is calculated using the weighted average ofcase and control p-hats. Delta p-hat is a reliable estimate of theallele frequency difference between the cases and controls.

Data Analysis by Genizon

Analysis of the data by Genizon was based upon Perlegen's p-hat values.

Data Analysis of P-Hat

The data consisted of 10 pools of cases and 10 pools of controls, with ap-hat value for each pool (provided by Perlegen's analysis methodsdescribed above). The weighted mean p-hat value for all case pools wascalculated as X=N_(i)Phat_(i)/ΣN_(i) where N_(i) is the number ofalleles (2× the number of people) in each case pool and the weightedp-hat value for all control pools were calculated asY=ΣL_(j)Phat_(j)/ΣL_(j) where L_(i) is the number of alleles in eachcontrol pool. The estimate of delta-phat, the difference in allelefrequency between cases and controls was X- Y and the significance ofdelta-phat was determined using a Student's t statistic where

$t = \frac{\overset{\_}{X} - \overset{\_}{Y}}{\sqrt{S_{p}\left( {\left( {\sum N_{i}} \right)^{- 1} + \left( {\sum L_{i}} \right)^{- 1}} \right)}}$

where S_(p) is the pooled variance under the assumption that both caseand control variances are equal. The variance of X was calculated asS_(X)=(¹/₈₋₁)ΣN_(i)(Phat_(i)− X)² and similarly the variance of Y wascalculated as S_(Y)=(¹/₈₋₁)ΣL_(j)(Phat_(j)− Y)². The Student's t testwas performed with 8+8−2=14 degrees of freedom. Single Marker P valueswere calculated for all markers within the genome wide scan map.

Combined P Values

In addition to single marker P values, combined P values acrossmulti-marker sliding windows were calculated after the method of Fisher(Statistical Methods for Research Workers, 14^(th) edition Hafner PressN.Y. 1970 pp 99-100) where χ²=lnP_(i) with 2 k degrees of freedom whereP_(i) is the P value for association of each of k markers within thesliding window. The combined P values identify regions of multiplesingle marker associations. However, Fisher's method assumesindependence of association for each marker which is known not to be thecase because of strong LD between adjacent markers. Therefore themagnitude of the combined P value was affected both by LD andassociation and these cannot be taken at face value. The combined Pvalues were best used as a means to identify regions of multi-markerassociation which can then be assessed on the basis of the magnitude ofsingle marker association.

Permutation Test for Exact P Values

There were 10 case and 10 control pools, each giving an estimate ofp-hat. Therefore there are 20!(20 factorial)/10!×10!=184756 ways togroup the pools into 2 groups of 10. The POOLEX exact test calculatesthe mean difference in phat for single markers between the two groupsfor all of these possible arrangements of the pools into two groups aswell as the combined P values for 5, 9, and 15 marker windows for allcombinations. These values are then arranged in order in each case andthe relative rank among all combinations of the actually observedcombination for real cases and controls is determined. The exact P valuefor the observed case and control combination is calculated as:

No. of combinations with a value greater than or equal to the actualobserved value 184756 total combinations

For example, if the actually observed combined P value for a 5 markerwindow corresponding to the actual cases and controls combination, werethe largest combined P value of all 184756 possible combinations of 2groups of 10, then the exact probability (P value) for observing thisevent by chance given no difference between cases and controls would be1/184756=0.000005412.

Example 4 Confirmatory Mapping and Fine Mapping

42 of the 47 top regions identified as being associated with longevityby the GWS are further analyzed by confirmatory mapping (genotyping allcases and controls samples individually) followed by fine mapping usinga denser set of markers, in order to validate and/or refine the signal.Both confirmatory and fine mapping are carried out using the IlluminaBeadStation 500GX SNP genotyping platform. Alleles are genotyped usingan allele-specific elongation assay that involves ligation to alocus-specific oligonucleotide. The assay is performed directly ongenomic DNA at a highly multiplex level and the products are amplifiedusing universal oligonucleotides. For each candidate region, a set ofSNP markers is selected with an average inter-marker distance varyingwith the mean extent of LD throughout the region as determined bydelta-M (Δ_(M)), where M is the number of markers present in the 300 kbwindow centered at each reference marker is defined as the square rootof the average r² or Δ² _(ij) measures of LD between all ({M(M−1)}/2)pairwise comparisons of all (M) markers within the 300 kb window (Dawsonet al., 2002). This produces an average multi-marker measure of LDanalogous to Hill's Δ statistic for two marker LD. Regions with a signalharboring a high −Log₁₀ P value and with mean delta-M of 0.3 or beloware mapped with a target density of one marker per 10 kb. Regionsshowing a signal with a high −Log₁₀ P value and with mean delta-Mbetween 0.3 and 0.35 as well as selected regions with a signal with alower −Log₁₀ P value and a delta-M value below 0.35 are mapped with atarget density of one marker per 10-20 kb. The principle is that low-LDregions will be mapped at a higher SNP density. Selected regions with adelta-M value above 0.35 are mapped with a density of one marker per20-30 kb, including the markers used in the GWS. The selected regionsare delimited by the location where the LDSTATS −Log₁₀ P values reachthe background level. The cohort consists of 615 cases and 615 controls(as used for the GWS).

Table 3 lists the fine mapping SNPs for the 42 confirmed regions andtheir respective p values using 615 cases and 615 controls trios and twoanalysis methods: LDSTATS(v4.0) and SingleType. For each region that wasassociated with longevity in the fine mapping analyses, we report inTable 7 the allele frequencies and the relative risk (RR) for thehaplotypes contributing to the best signal at each SNP in the region.The best signal at a given location was determined by comparing thesignificance (p-value) of the association with longevity for multiplewindow sizes, and selecting the most significant window. For a givenwindow size at a given location, the association with longevity wasevaluated by comparing the overall distribution of haplotypes in thecases with the overall distribution of haplotypes in the controls.Haplotypes with a relative risk greater than one increase the risk oflongevity while haplotypes with a relative risk less than one areprotective and decrease longevity.

Haplotype Association Analysis

Haplotype association analysis was performed using the program LDSTATS.LDSTATS tests for association of haplotypes with the disease phenotype.The algorithm LDSTATS (v4.0) defines haplotypes using multi-markerwindows that advance across the marker map in one-marker increments.Windows can contain any odd number of markers specified as a parameterof the algorithm. Other marker windows can also be used. At eachposition the frequency of haplotypes in cases and controls wascalculated and a chi-square statistic was calculated from case controlfrequency tables. LDSTATS v4.0 calculates significance of chi-squarevalues using a permutation test in which case-control status is randomlypermuted until 350 permuted chi-square values are observed that aregreater than or equal to chi-square value of the actual data. The Pvalue is then calculated as 350/the number of permutations required.

Singletype Analysis

The SINGLETYPE algorithm assesses the significance of case-controlassociation for single markers using the genotype data from thelaboratory as input in contrast to LDSTATS single marker windowanalyses, in which case-control alleles for single markers fromestimated haplotypes are used as input. SINGLETYPE calculates P valuesfor association for both alleles, 1 and 2, as well as for genotypes, 11,12, and 22, and plots these as −log₁₀ P values for significance ofassociation against marker position.

Example 5 Gene Identification and Characterization

A series of gene characterization steps was performed for each candidateregion described in Table 1. Any gene or EST mapping to the intervalbased on public map data or proprietary map data was considered as acandidate longevity gene. The approach used to identify all geneslocated in the critical regions is described below.

Public Gene Mining

Once regions were identified using the analyses described above, aseries of public data mining efforts were undertaken, with the aim ofidentifying all genes located within the critical intervals as well astheir respective structural elements (i.e., promoters and otherregulatory elements, UTRs, exons and splice sites). The initial analysisrelied on annotation information stored in public databases (e.g. NCBI,UCSC Genome Bioinformatics, Entrez Human Genome Browser, OMIM—see belowfor database URL information).

Database URLs Name URL Biocarta http://www.biocarta.com/ BioCychttp://www.biocyc.org/ Bimolecular Interaction Network http://bind.ca/Database (BIND) Database of Interacting Proteinshttp://dip.doe-mbi.ucla.edu/ Gene Expression Omnibushttp://www.ncbi.nlm.nih.gov/geo/ Human Genome Browserhttp://www.ensembl.org/Homo_sapiens/ Intercomhttp://interdom.lit.org.sg/help/term.php Kyoto Encyclopedia of Genes andhttp://www.genome.jp/kegg/ Genomes (KEGG) Molecular InteractionsDatabase http://mint.bio.uniroma2.it/mint/ (MINT) National Center forBiotechnology http://www.ncbi.nlm.nih.gov/ Information (NCBI) OnlineMendelian Inheritance inhttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=OMIM Man (OMIM) OmniVizhttp://www.omniviz.com/applications/omni_viz.htm Pathway Enterprisehttp://www.omniviz.com/applications/pathways.htm Reactomehttp://www.reactome.org/ Transpathhttp://www.biobase.de/pages/products/transpath.html UCSC GenomeBioinformatics http://genome.ucsc.edu/index.html?org=Human UniGenehttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=unigene

For some genes the available public annotation was extensive, whereasfor others very little was known about a gene's function. Customizedanalysis was therefore performed to characterize genes that correspondedto this latter class. Importantly, the presence of rare splice variantsand artifactual ESTs was carefully evaluated. Subsequent clusteranalysis of novel ESTs provided an indication of additional gene contentin some cases. The resulting clusters were graphically displayed againstthe genomic sequence, providing indications of separate clusters thatmay contribute to the same gene, thereby facilitating development ofconfirmatory experiments in the laboratory. While much of thisinformation was available in the public domain, the customized analysisperformed revealed additional information not immediately apparent fromthe public genome browsers.

A unique consensus sequence was constructed for each splice variant anda trained reviewer assessed each alignment. This assessment includedexamination of all putative splice junctions for consensus splicedonor/acceptor sequences, putative start codons, consensus Kozaksequences and upstream in-frame stops, and the location ofpolyadenylation signals. In addition, conserved noncoding sequences(CNSs) that could potentially be involved in regulatory functions wereincluded as important information for each gene. The genomic referenceand exon sequences were then archived for future reference. A masterassembly that included all splice variants, exons and the genomicstructure was used in subsequent analyses (i.e., analysis ofpolymorphisms).

An important component of these efforts was the ability to visualize andstore the results of the data mining efforts. A customized version ofthe highly versatile genome browser GBrowse (http://www.gmod.org/) wasimplemented in order to permit the visualization of several types ofinformation against the corresponding genomic sequence. In addition, theresults of the statistical analyses were plotted against the genomicinterval, thereby greatly facilitating focused analysis of gene content.

Computational Analysis of Genes and GeneMaps

In order to assist in the prioritization of candidate genes for whichminimal annotation existed, a series of computational analyses wereperformed that included basic BLAST searches and alignments to identifyrelated genes. In some cases this provided an indication of potentialfunction. In addition, protein domains and motifs were identified thatfurther assisted in the understanding of potential function, as well aspredicted cellular localization.

A comprehensive review of the public literature was also performed inorder to facilitate identification of information regarding thepotential role of candidate genes in the pathophysiology of longevitytrait and/or age-associated disorders. In addition to the standardreview of the literature, public resources (Medline and other onlinedatabases) were also mined for information regarding the involvement ofcandidate genes in specific signaling pathways. The Ingenuity PathwayAnalysis System was also used to generate protein interaction networks.A variety of pathway and yeast two hybrid databases were mined forinformation regarding protein-protein interactions. These included BIND,MINT, DIP, Interdom, and Reactome, among others. By identifyinghomologues of genes in the longevity candidate regions and exploringwhether interacting proteins had been identified already, knowledgeregarding the GeneMaps for longevity was advanced. The pathwayinformation gained from the use of these resources was also integratedwith the literature review efforts, as described above.

3. Expression Studies

In order to determine the expression patterns for genes, relevantinformation was first extracted from public databases. The UniGenedatabase, for example, contains information regarding the tissue sourcefor ESTs and cDNAs contributing to individual clusters. This informationwas extracted and summarized to provide an indication in which tissuesthe gene was expressed. Particular emphasis was placed on annotating thetissue source for bona fide ESTs, since many ESTs mapped to Unigeneclusters are artifactual. In addition, SAGE and microarray data, alsocurated at NCBI (Gene Expression Omnibus), provided information onexpression profiles for individual genes. Particular emphasis was placedon identifying genes that were expressed in tissues known to be involvedin the pathophysiology of longevity trait and/or age-associateddisorders.

4. Polymorphism Analysis

Polymorphisms identified in candidate genes, including those from thepublic domain as well as those identified by sequencing candidate genesand regions, are evaluated for potential function. Initially,polymorphisms are examined for potential impact upon encoded proteins.If the protein is a member of a gene family with reported 3-dimensionalstructural information, this information is used to predict the locationof the polymorphism with respect to protein structure. This informationprovided insight into the potential role of polymorphisms in alteringprotein or ligand interactions, as well as suitability as a drug target.In a second phase of analysis we evaluate the potential role ofpolymorphisms in other biological phenomena, including regulation oftranscription, splicing and mRNA stability, etc. There are many examplesof the functional involvement of naturally occurring polymorphisms inthese processes. As part of this analysis, polymorphisms located inpromoter or other regulatory elements, canonical splice sites, exonicand intronic splice enhancers and repressors, conserved noncodingsequences and UTRs are localized.

Example 6 SNP and Polymorphism Discovery (SNPD)

Candidate genes and regions are selected for sequencing in order toidentify all polymorphisms. In cases where the critical interval,identified by fine mapping, was relatively small (˜50 kb), the entireregion, including all introns, is sequenced to identify polymorphisms.In situations where the region is large (>50 kb), candidate genes areprioritized for sequencing, and/or only functional gene elements(promoters, exons and splice sites) are sequenced.

The samples to be sequenced are selected according to which haplotypescontribute to the association signal observed in the region. The purposeis to select a set of samples that covered all the major haplotypes inthe given region. Each major haplotype must be present in a few copies.The first step therefore consisted of determining the major haplotypesin the region to be sequenced.

Once a region is defined with the two boundary markers, all the markersused in fine mapping that are located within the region are used todetermine the major haplotypes. Long haplotypes covering the wholeregion are thus inferred using the middle marker as an anchor. Theresults included two series of haplotype themes that define the majorhaplotypes, comparing the cases and the controls. This exercise isrepeated using an anchor in the peripheral regions to ensure that majorhaplotype subsets that are not anchored at the original middle markerare not missed.

Once the major haplotypes are determined as described above, appropriategenomic DNA samples are selected such that each major haplotype andhaplotype subset are represented in at least two to four copies.

The protocol includes the following steps, once a region is delimited:

Primer Design

The design of the primers is performed using a proprietary primer designtool. A primer quality control is included in the primer design process.Primers that successfully passed the control quality process weresynthesized by Integrated DNA Technologies (IDT). The sense andanti-sense oligos are separated such that the sense oligos are placed onone plate in the same position as their anti-sense counterparts are onanother plate. Two additional plates are created from each storageplate, one for use in PCR and the other for sequencing. For PCR, thesense and anti-sense oligos of the same pair are combined in the samewell to achieve a final concentration of 1.5 μM for eacholigonucleotide.

PCR Optimization

PCR conditions are optimized by testing a variety of conditions thatincluded varying salt concentrations and temperatures, as well asincluding various additives. PCR products are checked for robustamplification and minimal background by agarose gel electrophoresis.

PCR on Selected Samples

PCR products to be used for sequencing are amplified using theconditions chosen during optimization. The PCR products are purifiedfree of salts, dNTPs and unincorporated primers by use of a MultiScreenPCR384 filter plate manufactured by Millipore. Following PCR, theamplicons are quantified by use of a lambda/Hind III standard curve.This is done to ensure that the quantity of PCR product required forsequencing had been generated. The raw data was measured against thestandard curve data in Excel by use of a macro.

Sequencing

Sequencing of PCR products is performed by DNA Landmarks using ABI 3730capillary sequencing instruments.

Sequence Analysis

The ABI Prism SeqScape software (Applied Biosystems) is used for SNPidentification. The chromatogram trace files were imported into aSeqScape sequencing project and the base calling is automaticallyperformed. Sequences are then aligned and compared to each other usingthe SeqScape program. The base calling is checked manually, base bybase; editing was performed if needed.

Example 7 Ultra Fine Mapping (UFM)

Once polymorphisms are identified by sequencing efforts as described inExample 6, additional genotyping of all newly found polymorphisms isperformed on the samples used in the fine mapping studies. Various typesof genotyping assays may need to be utilized based on the type ofpolymorphism identified (i.e., SNP, indel, microsatellite). The assaytype can be, but is not restricted to, Sentrix Assay Matrix on IlluminaBeadStations, microsatellite on MegaBACE, SNP on ABI or Orchid. Thefrequencies of genotypes and haplotypes in cases and controls areanalyzed in a similar manner as the GWS and fine mapping data. Byexamining all SNPs in a region, polymorphisms are identified thatincrease an individual's susceptibility to longevity. The goal ofultra-fine mapping is to identify the polymorphism that is mostassociated with disorder phenotype as part of the search for the actualDNA polymorphism that confers susceptibility to disorder. Thisstatistical identification may need to be corroborated by functionalstudies.

Example 8 Confirmation of Candidate Regions and Genes in a GeneralPopulation

The confirmation of any putative associations described in Example 7 isperformed in an independent general population patient sample. These DNAsamples consist of at least 400 male controls and 400 male patients withlongevity.

All publications, patents and patent applications mentioned in thespecification and reference list are herein incorporated by reference intheir entirety for all purposes. Various modifications and variations ofthe described method and system of the invention will be apparent tothose skilled in the art without departing from the scope and spirit ofthe invention. Although the invention has been described in connectionwith specific preferred embodiments, it should be understood that theinvention as claimed should not be unduly limited to such specificembodiments. Indeed, various modifications of the described modes forcarrying out the invention that are obvious to those skilled inmolecular biology, genetics, or related fields are intended to be withinthe scope of the following claims.

The practice of the present invention will employ, unless otherwiseindicated, conventional techniques of cell biology, cell culture,molecular biology, transgenic biology, microbiology, recombinant DNA,and immunology, which are within the skill of the art. Such techniquesare explained fully in the literature. See, for example, MolecularCloningA Laboratory Manual, 2nd Ed., ed. by Sambrook, Fritsch andManiatis (Cold Spring Harbor Laboratory Press: 1989); DNA Cloning,Volumes I and H (D. N. Glover ed., 4); Oligonucleotide Synthesis (M. J.Gait ed., 1984); Mullis et al U.S. Pat. No. 4,683,195; Nucleic AcidHybridization (B. D. Hames & S. J. Higgins eds. 1984); Transcription AndTranslation (B. D. Haines & S. J. Higgins eds. 1984); Culture Of AnimalCells (R. 1. Freshney, Alan R. Liss, Inc., 1987); Immobilized Cells AndEnzymes (IRL Press, 1986); B. Perbal, A Practical Guide To MolecularCloning (1984); the treatise, Methods In Enzymology (Academic Press,Inc., N.Y.); Gene Transfer Vectors For Mammalian Cells (J. H. Miller andM. P. Calos eds., 1987, Cold Spring Harbor Laboratory); Methods InEnzymology, Vols. 154 and 155 (Wu et al. eds.), Immunochemical MethodsIn Cell And Molecular Biology (Mayer and Walker, eds., Academic Press,London, 1987); Handbook Of Experimental Immunology, Volumes I-IV (D. M.Weir and C. C. Blackwell, eds., 1986); Manipulating the Mouse Embryo,(Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1986).

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Patent

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TABLE 1 List of Longevity candidate regions identified from the genomewide scan and Fine Mapping association analyses. The first columndenotes the region identifier. The second and third columns correspondto the chromosome and cytogenetic band, respectively. The fourth andfifth columns correspond to the chromosomal start and end coordinates ofthe NCBI genome assembly derived from build 35 (B35). Region ChromosomeCytogenetic Band B35 Start B35 End 1 1 1q23-q25 172922500 173790737 2 11q42-q43 225466378 225987741 3 1 1q42.13-q43 227029796 227560738 4 11q41-q44 236154391 236644953 5 2 2p24-p23 20891681 21414379 6 2 2p16.349817753 50101824 7 2 2p16.3 50101825 51414723 8 2 2q14.2-q14.3121573870 122352090 9 2 2q14.3 127494872 128057833 10 2 2q23-q24162067733 163003491 11 2 2q33 198533144 199078721 12 3 3p26.1-p25.16629288 8005030 13 3 3p21.33 32775326 33350489 14 3 3p21.3 3958226040686361 15 4 4q21 77038761 77559991 16 4 4q23 100854365 101421476 17 44q28.1 126424833 127003431 18 4 4q31.21 143068047 143957383 19 6 6p22.127042873 27452810 20 6 6q25 152286110 153283388 21 7 7p14.3 3032690230949204 22 7 7p14 32696611 33661735 23 7 7q31.3 127239803 127721306 247 7q35-q36 145001785 147803868 25 7 7q36.1 149454345 149955253 26 77q36.1 150075668 150561177 27 7 7q36 156602277 158116469 28 9 9p21.226700092 27297163 29 9 9q21.11 70132969 70960548 30 9 9q22.31 9317579893764675 31 9 9q31.3-q32 110265356 110882740 32 10 10p12.31 1989999920848770 33 10 10q23-q24 99966729 101219517 34 11 11p15.2 1169765112231441 35 11 11p15.1 19443295 20335244 36 11 11q21 95101359 9590884737 12 12q13 56066088 56635863 38 12 12q21.31 81345981 82268637 39 1414q21.3 49608345 50174491 40 14 14q31 80247029 80930316 41 16 16q12.146450036 46985312 42 16 16q22-q23 73578487 74088978 43 16 16q23.3-q24.176445659 78049764 44 18 18q11.2 22503963 23258373 45 18 18q22-q2361324317 61945465 46 22 22q11.21 16451100 17003269 47 X Xq13.3 8003075780606131

TABLE 2 Longevity genome wide association study results in the QuebecFounder Population (QFP). SNP markers found to be associated withlongevity from the analysis of genome wide scan (GWS) data. Columnsinclude: Region ID; Chromosome; Build 35 location in base pairs (bp);rs#, dbSNP data base (NCBI) reference number; Sequence ID, uniquenumerical identifier for this patent application; Sequence, 21 bp ofsequence covering 10 base pair of unique sequence flanking either sideof central polymorphic SNP; −log10 P values for GWS, −log10 of the Pvalue for statistical significance from the GWS for single SNP markers(both T test and Permutation test p-values are displayed; see Examplesection) and for the most highly associated multi-marker haplotypescentered at the reference marker and defined by the sliding windows ofspecified sizes (W05, W09 and W15). Poolex Single Single Marker Marker(Permutation Region ID Chr B35 Position RS# Seq ID Flanking Sequence (Ttest) test) W05 W09 W15 1 1 172922500 12758275 4805GCAGTTTACTRATTCTTAGAT 1.646 1.581 1.481 1.111 1.019 1 1 1729510951553770 4806 AAATGCTCAGYCTGTCTAATT 1.216 1.269 1.377 1.148 1.035 1 1172953249 1858537 4807 GGGTCCTGGAWTGATGGTGTA 1.153 1.099 1.170 0.9250.952 1 1 172969990 352333 4808 GTGCATCTGARAATAGAGGCT 0.063 0.068 0.7881.123 0.923 1 1 172980523 — 4809 CCTGCTTTATRAAGACTATAT 0.027 0.028 0.4091.010 0.914 1 1 173002087 181280 4810 CAAAAATACAWGTGTTTTTCA 0.754 0.6990.323 0.599 0.840 1 1 173006422 1503120 4811 ATAGAGGGGTYATTTGCAAGT 0.2360.225 0.311 0.421 0.843 1 1 173016407 2504511 4812 GTCTAGTGTCRCTTGGCTGTT0.825 0.856 0.398 0.265 0.787 1 1 173024350 — 4813 CAAGTCAAACMGATTTTAAGC0.113 0.111 0.347 0.368 0.664 1 1 173030154 2455729 4814GATCGACATAMGTCTGCACAC 0.202 0.204 0.458 0.465 0.835 1 1 1730370682455727 4815 CAAAGTGAGARTAGAATGAGA 0.577 0.575 0.298 0.346 0.863 1 1173049316 2455760 4816 GACTGTGCTGSATTAACCAAT 0.450 0.454 0.406 0.5630.859 1 1 173067383 1354286 4817 GAAAACTATAYATCATTGTAA 0.590 0.550 0.4080.856 0.947 1 1 173077283 12139848 4818 CCCTGAGGAARAAAAGAAGTA 0.4100.404 0.588 1.148 0.920 1 1 173085783 1503124 4819 GCTTGGTCCARTGTGTTTTGG0.242 0.237 1.316 1.144 0.927 1 1 173119991 2455725 4820ACAGGGTTTTSTTGCATAGAC 1.016 1.023 1.505 1.177 0.724 1 1 1731349011503116 4821 TGTGAAAAGARCCTACTGTTC 2.096 2.074 1.360 1.268 1.032 1 1173143278 4652175 4822 TTTGAGGCAGRATTTTATAAA 1.293 1.312 1.588 1.1481.586 1 1 173146438 4650943 4823 TTGTAGAATTRTGACTAGTCT 0.204 0.202 1.6651.015 1.486 1 1 173157471 6425384 4824 CTTAAAATGGRTATTTTTACT 0.000 0.0000.569 1.539 1.449 1 1 173164621 — 4825 ATCCTCTGCASACACCTTCTC 0.000 0.0000.025 2.137 1.387 1 1 173171836 12742614 4826 AGGTGATAGCYTGTGATATGC0.143 0.139 0.552 1.318 1.503 1 1 173177355 — 4827 AATCTGTCTGSCACGCCTGCC0.031 0.028 1.454 0.776 1.851 1 1 173188880 6676641 4828TGCATGATATKGTTGTTTCTC 1.511 1.420 1.185 0.872 1.813 1 1 1731947051569639 4829 CTTAGTTAGAKAATTTTTTTG 2.272 2.155 1.118 0.951 1.433 1 1173204977 10157031 4830 CTAAGCAGCARTTTTTGAAAA 0.011 0.011 1.340 1.2641.168 1 1 173219357 10489473 4831 TAATGCCTTTMAGTGCAGTTA 0.088 0.0880.952 1.445 1.080 1 1 173227979 6692448 4832 GATATTGTATYGAAGAAGAAC 0.4040.395 0.508 1.651 0.990 1 1 173235591 7530550 4833 GCTTCAACTGSAAGTATATAG0.710 0.769 0.909 1.298 0.905 1 1 173245194 2206510 4834GATTTGGAAAYACTGATGGCC 1.288 1.230 1.331 0.655 1.057 1 1 1732568512294654 4835 AAGGACTAAARTACAGCAAGA 0.866 0.857 1.266 0.682 1.188 1 1173262289 1569638 4836 CAGTTTGCTARATTGTAAAAT 0.825 0.861 0.930 0.6930.913 1 1 173268546 10913204 4837 TACTGAGAGTRACTGGTCCTG 0.736 0.7220.543 0.848 0.498 1 1 173274944 10798461 4838 ACAAGGCAGGWTTTGCCAGCT0.050 0.048 0.270 0.772 0.533 1 1 173280130 10489475 4839GTTGTATGCTSTTCCCATTAG 0.080 0.082 0.244 0.476 0.913 1 1 17329869316849993 4840 CTCCTCAGAGYTTCCCTTTAT 0.093 0.092 0.123 0.353 0.958 1 1173303088 240104 4841 TTGCCCATGGYACACTCCCCT 0.875 0.869 0.195 0.1630.793 1 1 173314633 10489479 4842 GTAATGTGACYTCCAGGAAAT 0.000 0.0000.350 0.363 0.558 1 1 173328155 — 4843 AAATTCTAGAYAAGAATCTGA 0.284 0.2850.370 0.509 0.460 1 1 173339083 6670258 4844 CAAACACAATRCACATTAGAA 0.4590.457 0.718 0.556 0.323 1 1 173353855 6689901 4845 TTAGTCACTTWGTCTGTTACT0.151 0.146 0.718 0.671 0.344 1 1 173364672 — 4846 TTGTCTTTCTWAGTATCTGTA1.559 1.512 0.688 0.486 0.397 1 1 173370788 953541 4847GACGCTGTGAWAGGAACATTC 0.000 0.000 0.643 0.410 0.524 1 1 17337865717546000 4848 CCATAGATAGRATAATGAAAA 0.218 0.218 0.772 0.581 0.510 1 1173385960 10494501 4849 CAGACTGCACRTAGAATAATA 0.405 0.401 0.190 0.5320.857 1 1 173391904 726252 4850 CTGTCTCTCAYGCCTTCCTTG 0.355 0.361 0.3410.678 1.087 1 1 173403942 791030 4851 TGAACAAATGRCCTATTCTGC 0.201 0.2120.364 0.248 1.430 1 1 173409624 17354671 4852 TGAACATGAARAAAAGGAAAT0.857 0.851 0.452 0.749 1.994 1 1 173424493 1995651 4853CCCAAAAAAAWTTCTATCATT 0.348 0.319 0.308 1.167 3.026 1 1 17344527010913246 4854 GACTCAGTAGSATTTTACCTT 0.656 0.630 1.205 1.437 2.702 1 1173455106 10494502 4855 TAGTGACTTAYCATAGTTCCT 0.037 0.036 1.361 1.9992.766 1 1 173462360 17352504 4856 ACTATTAGATRTGCTATGTTT 2.261 2.1771.735 2.812 2.955 1 1 173469910 1325601 4857 CAGAAACTGTKTAGAGCAGGC 1.4611.398 2.220 2.840 2.850 1 1 173475519 1325599 4858 TTATTTCCCCRCATGAAAATG1.434 1.360 3.143 2.892 3.454 1 1 173481447 10913248 4859TGGCAGCTCTSTCAGGTCAGT 2.400 2.348 3.044 3.065 3.198 1 1 17349168110913254 4860 TTTAAGCAGAMAGGCAAAAAC 4.146 3.804 3.289 3.039 3.198 1 1173503616 760939 4861 TATTCTCTTGRCATAAGGATT 0.618 0.587 3.542 3.7483.130 1 1 173510267 10913257 4862 CTTCTCTGTTKTCTTGGACCT 1.061 1.0322.688 3.526 3.369 1 1 173522159 10732997 4863 ATTATTTGAARTAGATTTGAA0.604 0.590 2.231 3.496 3.262 1 1 173525723 1325596 4864GACCCTGATGRCACTACAGCT 0.299 0.296 1.676 2.522 2.888 1 1 17353900016850237 4865 AGATTAGAAGYGACCCTTGTT 2.965 3.014 1.463 1.778 2.700 1 1173548489 6681309 4866 AGTCAGAATTKAGTCTGGTAG 0.471 0.464 1.283 1.4002.492 1 1 173558611 16850265 4867 CAGGACAAAASGGCATGTAAG 0.386 0.3722.165 1.157 1.934 1 1 173566293 4652196 4868 GATCCTCTCAYTCAGTTGTGT 0.0100.010 0.584 1.038 1.090 1 1 173572622 16850279 4869CAATTGCAGGYTCACAGAAAC 1.855 1.775 0.467 1.104 0.992 1 1 17357975916850292 4870 GTAGGAAGTCYGTCCTCTGCC 0.026 0.026 0.381 0.309 0.767 1 1173586269 4652202 4871 TTCAAGATGASTGGATTCTGG 0.176 0.177 0.573 0.3630.809 1 1 173592666 — 4872 AAGATGTAGAYACACACACAA 0.149 0.149 0.039 0.3470.767 1 1 173599953 12131188 4873 TAAATAAATGYCATTTGCTTT 0.494 0.4880.211 0.350 0.296 1 1 173606740 11585886 4874 CTGGACATGGRAGGAAACAAA0.130 0.130 0.245 0.132 0.302 1 1 173613449 10489303 4875TAGGTCATACRGATTTCAAAG 0.751 0.746 0.238 0.170 0.318 1 1 173624043 7606904876 AATCATTTGCYGAGTTTATAG 0.294 0.292 0.327 0.220 0.348 1 1 17363249517379085 4877 AAAACCCACAYGGAAGTCACC 0.043 0.041 0.316 0.311 0.112 1 1173637961 17313493 4878 GTTGCAGTAAYTGACTCAGCC 0.751 0.750 0.225 0.3010.111 1 1 173651588 4652211 4879 TGTATACATAYCGCCAACAAG 0.135 0.135 0.3210.311 0.139 1 1 173660586 2235300 4880 ACCTACTTCTYTGACCAGTTG 0.459 0.4480.468 0.173 0.176 1 1 173667964 16850479 4881 TACCTAGAGGYAAGAATTGGC0.533 0.513 0.244 0.124 0.252 1 1 173674919 — 4882 TATATGTGCASACAGGTGCTT0.000 0.000 0.230 0.205 0.354 1 1 173682085 — 4883 TCTCCTTCAGSACCTACAGCT0.280 0.268 0.093 0.133 0.278 1 1 173691633 228006 4884TCAGCCTAAAYGTTTCTTTCT 0.126 0.128 0.093 0.320 0.426 1 1 173703286 2280154885 ATAAAACCAGSAGTATGAGCA 0.001 0.002 0.132 0.389 0.405 1 1 1737101226661023 4886 GGTCTTTGTCYTTTTAGTGGT 0.449 0.448 0.366 0.340 0.275 1 1173724072 13375529 4887 CAGGCAGTCCRCATGGTCTAG 0.533 0.525 0.583 0.5160.355 1 1 173730025 10753142 4888 CTTGCCTAGCWCAAACCCAAG 1.058 0.9910.736 0.432 0.378 1 1 173735903 — 4889 TGTTCAAAAGRTTCCTCACTT 0.685 0.7050.999 0.425 0.278 1 1 173741051 6672689 4890 GTCTTTAATCRTGTGGAAAGG 0.3200.319 0.727 0.601 0.483 1 1 173748025 6667588 4891 GTCATGGGGCRAAACTGAATT1.036 1.035 0.358 0.657 0.552 1 1 173767376 — 4892 CCAAGTGCCCKGTTCCCTCTT0.003 0.003 0.324 0.482 0.566 1 1 173773076 6704507 4893AGTTATTAACYTATTCACACT 0.148 0.148 0.427 0.615 0.597 1 1 1737843952068331 4894 GTTTCTAACCYCTGGAATAAA 0.580 0.593 0.093 0.561 0.516 1 1173790737 1028304 4895 AACATCAAAAYCATCAGTTGT 0.570 0.583 0.599 0.5240.466 2 1 225466378 537250 4896 ACAGATACTCRGTCGGCCCAG 0.098 0.096 0.0930.330 0.319 2 1 225472223 484947 4897 ATTGAGCCTGMTCAGTAATGA 0.249 0.2560.058 0.139 0.319 2 1 225479994 627512 4898 ATCATACATTYTCTACTTCGT 0.1370.136 0.079 0.101 0.306 2 1 225488350 1380024 4899 CTTAATTCTAYTTGAGTCACC0.111 0.110 0.083 0.240 0.316 2 1 225496212 682283 4900GATCCAGGGTRTTATGATGGA 0.496 0.494 0.134 0.217 0.165 2 1 22550462612122684 4901 ATTACCCTTTYCTGTGCCCTT 0.139 0.139 0.415 0.231 0.427 2 1225511143 1359150 4902 AAGGCCATAGSTAGGAGCATG 0.484 0.464 0.505 0.2080.490 2 1 225519198 630685 4903 GGAACCTGCARTGAGAAGTAA 1.062 1.094 0.4110.194 0.400 2 1 225531481 12139882 4904 TTGTCACTCAYAGGGCCAATG 0.3300.344 0.431 0.906 0.494 2 1 225537876 619422 4905 GGAAAACCCAMAGAAGAATAT0.225 0.239 0.294 0.939 0.664 2 1 225610045 — 4906 AATCCAGCTCRCAAACCAAGA0.217 0.220 0.967 0.960 0.744 2 1 225616273 9435811 4907CCTCTAATGGSTTGGTCACAC 0.131 0.130 1.032 1.009 0.904 2 1 22562328912026629 4908 TTCTTTCATGYATCATGTAGA 2.452 2.384 0.927 0.944 0.884 2 1225626459 — 4909 CACGCCTTTCYAGCCTCACAC 0.596 0.592 1.169 0.927 3.052 2 1225633768 342782 4910 ATTTATGTATKCAGGTATTCA 0.030 0.030 1.729 1.0414.062 2 1 225645683 342785 4911 AGGAGAAGAGMCTTCAAACTG 0.557 0.551 0.4551.205 3.966 2 1 225660552 — 4912 CACATCTACCRACCATCTGAC 0.927 0.883 0.4143.775 3.710 2 1 225667166 — 4913 AGTGGAGGTCRTTAGGAGAGG 0.319 0.312 0.6033.761 3.447 2 1 225673850 342818 4914 ACATTTAAATYATCCAGTCTG 0.513 0.5003.447 3.735 4.665 2 1 225680359 7526949 4915 CCCATTGTTCRTTTTTGGAAT 0.4410.436 3.789 4.364 3.966 2 1 225687492 12041301 4916AGACAACATCRTGTTCTTCCA 4.536 2.865 3.687 3.761 3.221 2 1 22569297216849638 4917 TCATCTCTTAWGCAACTGAAC 2.478 2.365 3.966 3.108 2.686 2 1225703254 — 4918 GCACATTTCAYCAAGCTGATA 0.397 0.398 3.397 2.886 2.545 2 1225722322 — 4919 TGAATATAATYATAAACTGAG 1.197 1.054 1.447 3.327 2.372 2 1225730168 9435835 4920 AAGGATCTTASCCTGCATTTT 0.150 0.153 0.328 2.8472.310 2 1 225738651 237777 4921 TTACATTACAYTTCTTGACAT 0.030 0.031 0.8721.260 2.290 2 1 225746316 9069 4922 TTGGGACACAWTCGTTGGAAC 0.199 0.1970.484 0.525 2.409 2 1 225756176 9435838 4923 TCAAAGCCGCWTGAGAAGGTG 1.7191.730 0.550 0.591 2.457 2 1 225765367 237799 4924 TTGGGTTCTCYGTTTCAGACT0.279 0.276 0.571 0.292 0.955 2 1 225771581 10916465 4925AGGACCAAGASTAAAGGGAAA 0.294 0.274 0.790 0.443 0.402 2 1 22580718316849715 4926 AGGAATTCAAWCCTCAAGACC 0.139 0.136 0.117 0.487 0.391 2 1225815451 238098 4927 GAGCCAGGGTYCAGTTTGTGC 0.652 0.637 0.245 0.4850.315 2 1 225829949 6587333 4928 GTCATAAATGRGTATTTGTTA 0.000 0.000 0.2270.143 0.316 2 1 225843520 12131147 4929 TGTGCCATTCSAATGTGGCTT 0.7080.712 0.255 0.153 0.418 2 1 225845432 683116 4930 ATCTGGATAGWGATCACTGAG0.242 0.241 0.194 0.283 0.377 2 1 225862235 4925479 4931AATGTCAACTYGATTTTAGAT 0.207 0.212 0.272 0.297 0.097 2 1 225867901 4824424932 GTTACAGACAMACAAGTTAAG 0.416 0.418 0.331 0.273 0.082 2 1 225872563500639 4933 AAACGAGTTTRTTACTTTGAG 0.328 0.328 0.324 0.256 0.155 2 1225894215 — 4934 CTTACAGCACWCAAAAGATAA 0.903 0.848 0.423 0.122 0.204 2 1225902995 1328219 4935 CTTCTCAGGTRTATGACAGTA 0.154 0.158 0.278 0.1060.147 2 1 225909019 7555749 4936 CAACCTTATAMATTATCGGCA 0.525 0.524 0.1630.225 0.194 2 1 225914901 4925489 4937 GTGAAGCAATRGCTCTAGATT 0.047 0.0450.026 0.235 0.138 2 1 225921466 — 4938 GGATCTGATGYACAGTAGTCC 0.046 0.0380.194 0.211 0.172 2 1 225930073 17355666 4939 GACCGTCAACYGACACAAAAT0.099 0.099 0.167 0.133 0.219 2 1 225938469 11805194 4940CATTTACTGAYGTTTGAACTC 0.890 0.884 0.209 0.144 0.185 2 1 22594720612033236 4941 GACTGAATTTYGCATATACAG 0.387 0.389 0.325 0.129 0.209 2 1225949387 6671385 4942 TTACATGGTCYTCAACAGCAG 0.126 0.124 0.342 0.2360.110 2 1 225971007 927205 4943 AAGAAAGTGAYTCCAGAGACA 0.484 0.438 0.2120.264 0.213 2 1 225982078 — 4944 CCCAGTCTGTYGAATGAAGAA 0.258 0.248 0.2870.341 0.220 2 1 225987741 927204 4945 GGGTCCTCTTYCAGAGGTTTG 0.499 0.5160.249 0.155 0.262 3 1 227029796 12135204 4946 ATCCATGATTRCAAGTGATTG0.273 0.270 0.441 0.265 0.471 3 1 227038868 200580 4947TTGGTTGTAARATCTTAGAAA 0.155 0.159 0.101 0.371 0.406 3 1 227056681 8534654948 CTAGAAATCCWGCAGACAGTA 0.351 0.352 0.187 0.466 0.267 3 1 227062143699900 4949 CTATTTACCASAAGCAAGCAT 0.232 0.237 0.255 0.257 0.298 3 1227068583 10779849 4950 TGCAGATGGTYTCACTCACTC 0.524 0.532 0.399 0.2300.309 3 1 227082261 3761950 4951 GCTAAGCCAGRCAGTAACCCT 0.464 0.476 0.4950.312 0.696 3 1 227102376 2225146 4952 GGTTGTTTTCRTTAGCAGCTG 0.663 0.6560.420 0.334 0.414 3 1 227108750 16852170 4953 CCTTCACTAAYGAGGCTAGAC0.612 0.597 0.467 0.265 0.389 3 1 227115479 2148964 4954GGCCTTTGCAKTACCCTCTCT 0.078 0.068 0.393 0.911 0.568 3 1 2271222162493144 4955 AAGAGTCATTKTTGCAGATTC 0.644 0.621 0.176 0.774 0.571 3 1227129065 2296800 4956 AAATGTATTAYGCTATGTTAT 0.268 0.301 0.868 0.5990.475 3 1 227135608 3789662 4957 AAATCCTCACRTTTTACAACA 0.141 0.139 0.8890.751 0.479 3 1 227147004 11122575 4958 AAAATCCTTCRTGTCATTTGC 1.9371.984 0.587 0.589 0.485 3 1 227153414 11122576 4959TTTCATTGTCYCAATATTCCC 0.263 0.254 0.900 0.541 0.402 3 1 2271600949804147 4960 CTTGGAAGAGRCATGTGAGCC 0.067 0.067 0.887 0.426 0.456 3 1227167331 7548604 4961 ACAGAATATTSCTGGCAAAAT 1.051 0.999 0.169 0.4900.410 3 1 227174605 2478518 4962 TTACTTATGTSCTGGTTTATG 0.179 0.178 0.1530.483 0.449 3 1 227186246 2478542 4963 GTGAAGCTTCYGAGCATTTGT 0.006 0.0060.285 0.223 0.525 3 1 227196981 2493148 4964 TTTACCTAAGYGCATCTCTGT 0.2520.262 0.048 0.250 0.540 3 1 227205229 2282319 4965 CCGACCACAAYGAGTTCTGGA0.527 0.528 0.256 0.293 0.903 3 1 227213689 6677009 4966TCTTGGACTCYGAAGAGTTGA 0.088 0.095 0.402 0.287 0.663 3 1 22721991911122597 4967 ATTCTTCACCRGCTCTAGGAT 0.911 0.895 0.397 0.349 0.818 3 1227231640 12082061 4968 GCATAACACAYGGCTAGCGCA 0.439 0.414 0.581 0.8191.742 3 1 227238473 11122604 4969 TTTCTAATCTRTGTTCCATAG 0.258 0.2650.678 1.044 2.642 3 1 227246905 10864783 4970 TAGTATACACRAGCTATCTAA0.969 0.975 1.022 1.241 2.983 3 1 227258633 1202525 4971TGAGGCTGCASGGAGGCCAGA 0.414 0.388 1.250 2.377 3.250 3 1 2272656701202534 4972 GTTTTCCCCCRAAAAGGTTGG 1.595 1.513 1.512 3.676 3.127 3 1227270465 — 4973 ATGGCCCCGTRAGGTTAGCGG 1.029 1.010 2.517 3.665 3.447 3 1227281064 6673201 4974 AGGTGTCGCARAGATTAAGGC 0.939 0.918 4.364 3.8863.585 3 1 227291324 16852841 4975 TGCTCAGCTCYAAAAACTCCA 3.457 3.2374.966 3.735 3.623 3 1 227301328 1999903 4976 CTCTGAGGAAYGAAGACTTAG 4.5654.153 4.966 4.011 3.534 3 1 227307747 16852927 4977ACCGGCTCCTYTCCTTTTGCT 1.713 1.578 4.364 4.011 3.386 3 1 2273200881202594 4978 TTTTTCCAATKGTAGAGAGAG 0.031 0.031 3.159 3.604 3.312 3 1227328505 4028814 4979 AATTCTCTACYTCAGAAAGCA 0.078 0.076 0.771 2.7592.944 3 1 227334767 6704527 4980 TTACTTCCTGKTAAGAGGTCT 0.800 0.818 0.1381.946 2.526 3 1 227343240 3762404 4981 AGACGGAAGTYAGAGATTTTT 0.581 0.5500.244 0.662 2.282 3 1 227354652 4846878 4982 AAGAAGGCCAYAGACCAGGAA 0.0530.054 0.544 0.238 2.167 3 1 227362159 11122622 4983GGGCAGTCTAYTTAGAAATTG 0.344 0.338 0.447 0.294 1.379 3 1 2273729027533671 4984 CAGTCATGGTRTAAAGGGCTC 0.797 0.812 0.288 0.314 0.347 3 1227381046 6667425 4985 GTCAGGCAGTYCTCAAGCAAT 0.624 0.586 0.348 0.2420.093 3 1 227388039 6673085 4986 CAGCTCTACTKCTTTATGCTA 0.091 0.092 0.2720.127 0.102 3 1 227394719 6679473 4987 TGGATGCCTCYAAATTATTCT 0.228 0.2380.161 0.187 0.108 3 1 227396393 16853489 4988 ATTAAGTTAAWCATGAAGATC0.146 0.147 0.048 0.129 0.046 3 1 227417148 7413309 4989TGTTGTTAAAYGGTATGTAAC 0.548 0.544 0.124 0.033 0.031 3 1 2274320266541249 4990 ATTTCAGACTYTTAAGAATTA 0.039 0.040 0.074 0.012 0.075 3 1227458465 4551581 4991 TAAATTACACMATAAATCATT 0.442 0.455 0.055 0.0130.071 3 1 227469378 4506448 4992 TGCTCAAAAAYCATGGAAGAA 0.008 0.008 0.0140.020 0.024 3 1 227486664 4332346 4993 ATTACTTTGARAGCAACTGTG 0.122 0.1190.022 0.063 0.052 3 1 227496553 6679453 4994 ATTAATTTACRCAAAGTCATC 0.1510.152 0.017 0.042 0.138 3 1 227511349 6663912 4995 CAAACTACCCMGAGTTAGCAC0.103 0.104 0.133 0.051 0.257 3 1 227518492 6541254 4996TTGGCATTCARTGATCTGAAA 0.375 0.388 0.190 0.139 0.234 3 1 2275410957518479 4997 GTTGCCTGTCRATGGCAGTCT 0.682 0.685 0.169 0.338 0.157 3 1227551201 9431925 4998 ACTTCCTTCCMTTCCGTTAGA 0.309 0.307 0.543 0.5800.191 3 1 227560738 10864669 4999 TCTGCAAACARATCAAAACAG 0.104 0.1060.746 0.539 0.134 4 1 236154391 16838637 5000 TCGGTCTCTGRAAAAAAATGG0.649 0.661 0.578 0.423 0.609 4 1 236161654 7541783 5001CTTCCTTTTGWCAGCAGGAGA 0.994 0.971 0.677 0.453 0.576 4 1 23616899610925949 5002 AGATTAATGTMCCTGATTTAA 0.471 0.482 0.601 0.467 0.443 4 1236176629 6688537 5003 ATGGCAGCCCMTGCCACTTTT 0.331 0.331 0.663 0.5290.478 4 1 236183565 2355237 5004 AAAGAATTAGRTGATCGATAC 0.280 0.277 0.3850.574 0.444 4 1 236196816 10802795 5005 CTCAATGTTCYGAATTATGAG 0.8440.802 0.348 0.545 0.484 4 1 236203307 6429154 5006 TTAATACGGCRGATTTCTTTC0.176 0.170 0.456 0.455 0.490 4 1 236209843 10925956 5007GCCACTCAACSCCAGACTTTT 0.321 0.315 0.516 0.444 0.453 4 1 2362175526657343 5008 TAACAAACACWTACTTCACAA 0.690 0.661 0.416 0.454 0.390 4 1236227047 11579382 5009 TGTTTGTAATSTTATGCTGCT 0.548 0.522 0.514 0.4010.617 4 1 236235692 11585281 5010 ATTTTTAAAAYTCTGTGATAC 0.000 0.0000.534 0.220 0.620 4 1 236237040 934344 5011 GTTTTCAAACYATTTTATAAG 0.0000.000 0.252 0.226 1.292 4 1 236245819 10925972 5012TACCAATTTAKTTCAGGGAGA 0.394 0.387 0.089 0.918 1.426 4 1 236257599 8652135013 ATCTTAGGATYAGCGTTCACT 0.115 0.113 0.091 0.873 1.613 4 1 236274676658842 5014 AAAACATTATWAAACCCGGTT 0.158 0.158 0.889 2.569 1.847 4 1236286446 6661317 5015 CAGAAACACARAATCAAATAC 0.343 0.351 1.017 2.3651.952 4 1 236300211 1110615 5016 TCTGAAAATCRCCATCTGTAA 2.208 2.317 3.7352.650 1.742 4 1 236311513 6429161 5017 TCTGCATAGAWATCTAATTCA 0.000 0.0003.687 2.691 1.509 4 1 236324113 477507 5018 CACCAACTAGRTTGAAAATTG 3.7413.312 3.924 2.327 1.509 4 1 236339319 613228 5019 TCCTTTTAATWTAAATATGTT0.297 0.379 2.589 2.459 1.749 4 1 236365881 17598757 5020ACTCTCTCTAYGTCTCTATAT 0.981 0.944 1.965 2.235 2.389 4 1 236372267 — 5021TCCTGGAGACWCATCAGCCCA 0.062 0.062 0.145 1.326 2.635 4 1 23638151410495448 5022 TTTTAATCCCRTAGAGCCAGA 0.043 0.043 0.079 1.654 2.886 4 1236391685 10926012 5023 TCGTCTCAAARTAGTCAAGTA 0.135 0.132 0.002 0.8073.173 4 1 236403350 4659554 5024 AGGGGCTCACRTCTCAGTTGC 0.021 0.021 0.2460.898 2.701 4 1 236415559 — 5025 TAATAGCACCKGAAATAAAGA 0.000 0.000 1.2310.930 2.549 4 1 236429275 12404902 5026 TCACCTCTTGYCAGTTAATAA 1.1951.253 1.706 1.466 0.868 4 1 236436879 6671685 5027 TCCAGCAAAAYCGTTTATTTT2.138 1.968 2.197 1.658 0.771 4 1 236443827 10926032 5028GATCTCACGTWGACTCTGTCT 0.000 0.000 2.299 1.874 0.555 4 1 2364503564659556 5029 ATACCCATTAYGTAGCTTTTC 0.935 0.986 1.995 1.793 0.541 4 1236456129 10926042 5030 TTAAAACATGYCTCATGTGAA 1.160 1.149 0.932 1.3890.530 4 1 236461974 4659557 5031 AGGGCGTGCARCTGAGCGGTG 0.000 0.000 0.6290.862 0.557 4 1 236467860 12744283 5032 TAGAACTTCCRTCCCTCATAA 0.1150.113 0.243 0.231 0.679 4 1 236474186 12407556 5033ACATGCATAGRTGTTGTTCTG 0.046 0.046 0.012 0.166 0.659 4 1 2364797002169453 5034 AAGAAAAAATYTACACTTGGA 0.051 0.053 0.003 0.062 0.458 4 1236485357 12743555 5035 TAGGTAGAAAKAGGATTACAG 0.139 0.143 0.003 0.0120.133 4 1 236493915 7512443 5036 AGGGAGTATTWATTGAGCAGT 0.050 0.042 0.0090.018 0.118 4 1 236500948 12073505 5037 TTGATGGGAGMCTTAGGACAT 0.1040.084 0.056 0.032 0.068 4 1 236508885 2883722 5038 TGAGGTTAAGRTTTAGAATTT0.225 0.226 0.103 0.039 0.013 4 1 236518457 10926084 5039TTTTCTACAAYGAATGATTCT 0.550 0.529 0.164 0.053 0.011 4 1 23652829512075485 5040 GTGAAGTCCCRCAAGAGAGGT 0.416 0.415 0.188 0.083 0.017 4 1236533851 12239766 5041 GAAAAAGTGCRCAAAATCTGC 0.334 0.323 0.202 0.0840.019 4 1 236545865 — 5042 CATCACGTTGMATTTTTAAAT 0.174 0.165 0.174 0.0950.033 4 1 236557741 — 5043 TAGAGATCCCYATAGTCTATC 0.261 0.284 0.100 0.1120.028 4 1 236561988 — 5044 AGCACCTTTTYCAGTAGTCTC 0.443 0.461 0.079 0.0660.035 4 1 236584566 12143985 5045 GTGACATTCAYGTATATAGTT 0.113 0.1160.116 0.065 0.164 4 1 236591444 16839420 5046 TTTTCCTACTRTTGAATATGC0.262 0.231 0.092 0.043 0.199 4 1 236597432 1545727 5047TCAGTGAATTWTGATTCCTCC 0.302 0.281 0.086 0.055 0.212 4 1 23660570916839444 5048 GCGAGATTATRGGAGAGAATA 0.168 0.169 0.075 0.276 0.272 4 1236625805 — 5049 TTTTAAGACAMTGACATACTT 0.431 0.423 0.094 0.278 0.240 4 1236632495 12071494 5050 TTGAGCTAACYGGCGCAACAT 0.114 0.117 0.539 0.4180.360 4 1 236638476 10737842 5051 AACATTGAACYTGCAGAATGT 0.331 0.3410.684 0.590 0.406 4 1 236644953 6666380 5052 TGAGAGCCCARAGTGATTGAG 1.5721.626 0.782 0.525 0.425 5 2 20891681 661929 5053 AGGAAACTCTRTGTTATTATG0.058 0.057 0.275 0.303 0.182 5 2 20901381 16988063 5054CTACTTCATCRATCCATAAGG 0.054 0.054 0.090 0.224 0.184 5 2 20910027 5350225055 CTCATACCACYAGAGAGACGT 0.347 0.326 0.098 0.197 0.168 5 2 2091634616982026 5056 TACTTCACCAYTTAAACCTCC 0.411 0.410 0.138 0.090 0.277 5 220926604 649018 5057 TGAAAATCAARCTCAGTCTAT 0.359 0.370 0.213 0.077 0.2835 2 20933936 16988079 5058 TCATGACTTCYCTGCTGCCAT 0.197 0.191 0.246 0.1270.243 5 2 20952607 340602 5059 TGACTGCCTGYTTCATTTGGT 0.217 0.221 0.1620.273 0.104 5 2 20968923 6709100 5060 GCCCTCCATAYGGTTCAGAGA 0.480 0.4950.167 0.342 0.108 5 2 20975686 6743521 5061 AAAGCCAATGRGCCTAAAATT 0.1720.171 0.361 0.277 0.153 5 2 20984059 11891188 5062 AAAGTCAGAGWTCTTCGGAGG0.478 0.485 0.540 0.202 0.156 5 2 20992564 1437404 5063AATATAAAAGYTAAAAGGCAA 0.824 0.828 0.413 0.237 0.164 5 2 2099869016988112 5064 TCCTCCTCCARCTCTTACGTT 0.603 0.604 0.376 0.305 0.166 5 221005714 28538171 5065 AAGACACCCAYGTCCTGAGAG 0.178 0.182 0.316 0.2010.115 5 2 21023010 6722139 5066 AGCACACATGMGGAATTTTAT 0.082 0.082 0.2040.245 0.174 5 2 21047370 4468779 5067 AAGAACCCATYCTAGCAGGTC 0.379 0.3720.068 0.230 0.179 5 2 21060496 4606879 5068 GTCCTAGGAARATGAAAAAGA 0.4730.479 0.112 0.088 0.240 5 2 21100722 17397826 5069 AGAATGATTGYGTTTTTGATT0.043 0.055 0.227 0.107 0.259 5 2 21107056 17041679 5070TAGAACAAATYTGTGCTGTTG 0.385 0.386 0.129 0.121 0.309 5 2 2111299417041694 5071 AGCTGCACAAYCCACCTTGCA 0.535 0.487 0.192 0.292 0.278 5 221124837 4665642 5072 CCAGTGGAGARTTTCCATCTC 0.021 0.022 0.259 0.2700.250 5 2 21137405 1042031 5073 ATATGGAATTYTTGAGTAACT 0.620 0.626 0.4110.335 1.064 5 2 21149196 673548 5074 CAAAAATACCRATTTGACAAG 0.161 0.1670.341 0.461 1.039 5 2 21157019 3791981 5075 TGATCTCTCCRGAGCTATTGT 0.9250.917 0.596 0.467 0.978 5 2 21162692 17240674 5076 CAAAATGTCTKGATTTCATTG0.285 0.281 0.609 1.536 0.919 5 2 21172253 17240549 5077ACTCCTCAATRACTGTTTTAA 0.802 0.757 0.703 1.628 0.947 5 2 21182206 5859675078 TTCTCTGGGAMCAGCCCAGTT 0.621 0.618 2.097 1.396 0.952 5 2 2118805311892073 5079 GCATAAACATRCGGAAATACC 0.404 0.403 2.202 1.345 0.915 5 221199203 — 5080 GCCGTCCATCYATCCGTTACT 3.828 3.687 1.756 1.193 1.102 5 221204658 594677 5081 ACTGAGTAATYGTCTAATGAA 0.075 0.075 1.382 1.173 1.0405 2 21217337 17399144 5082 ATGCTTCAATRAACAGCCAGT 0.198 0.196 1.386 1.0350.992 5 2 21223437 675430 5083 CATTTTAATGMGGACAAATCT 0.289 0.286 0.1301.080 1.056 5 2 21229137 645456 5084 CCTTCATCATYGATCTCTGGA 0.358 0.3340.234 1.106 1.138 5 2 21233518 312942 5085 GTATTCCTCCYTACCTGATTT 0.3490.350 0.451 0.202 1.344 5 2 21253964 312957 5086 ATGATTGATARTTAATTTACT0.484 0.451 0.479 0.437 1.386 5 2 21259931 — 5087 AGGGTTGTGTKAGGAAGAGAT0.898 0.878 0.355 0.520 1.409 5 2 21270933 1517471 5088TGTATAGTTCMTCCTAATATC 0.398 0.392 0.690 0.932 0.595 5 2 2128272011687710 5089 CAGTTCCTACYTTGTTGAGTT 0.038 0.037 0.750 1.161 0.724 5 221294098 17041836 5090 CATTAAGTCCRATCTGTCCTT 1.225 1.217 1.262 1.1270.740 5 2 21308834 506585 5091 CTCTACTAGGRCTACAAGCTT 0.519 0.527 1.6391.118 0.704 5 2 21312943 2030195 5092 CAATTTAATARAATCATTTTC 1.700 1.7531.595 0.980 0.650 5 2 21334933 4387830 5093 TATACAACTTRAGAAACAAAG 1.0320.994 1.041 0.963 0.578 5 2 21339837 312052 5094 TGCCTTCTATRGAATATGTCT0.113 0.111 1.080 0.964 0.536 5 2 21350446 437775 5095CCCATTTCAGRTTGCCTTTCT 0.179 0.178 0.341 0.555 0.683 5 2 2136068613420469 5096 TCTCACTCTGYTCAGATTGCT 0.568 0.567 0.110 0.421 0.829 5 221371828 10166647 5097 ATACATTTTGYCTGTGTTCTA 0.274 0.274 0.089 0.1010.998 5 2 21386917 4665243 5098 GCAAACAGCTRTCGCAAGCCA 0.189 0.188 0.0730.167 0.767 5 2 21395207 1397400 5099 GATTACAGAAKTCTCCTTTGT 0.034 0.0350.023 0.355 0.666 5 2 21403500 17042055 5100 TATGCATGCAKCCTGCACCTC 0.0940.090 0.293 0.515 0.404 5 2 21412996 4665662 5101 TGCAAATGAAKCTATTCCTGA0.211 0.208 0.590 0.459 0.229 5 2 21414379 13396400 5102GAAGGTGGAARTGGAATAAAA 1.514 1.392 0.856 0.411 0.400 6 2 49817753 10223695103 ATATAGCTCAMCAAAAATATT 0.450 0.424 1.326 0.817 0.697 6 2 4982032117180320 5104 TTAATAATTTSAGCACTGTCA 0.403 0.396 1.215 0.886 0.654 6 249826561 17180439 5105 ATTGAATTGASTCTTCCAGGG 1.506 1.592 0.680 1.0650.663 6 2 49856216 10185615 5106 TATTAATCTTMTTTGGACACG 0.007 0.007 0.6291.205 0.675 6 2 49869232 13407557 5107 ATGCAATGCARTAAAACACTG 0.648 0.6180.850 0.683 0.683 6 2 49876262 12713075 5108 TGGTTACTATYCTCTCCCCAG 0.3000.277 0.435 0.583 0.786 6 2 49894129 6737293 5109 TGTTCCTTCTYTTTGGGCATA0.901 0.906 0.599 0.615 1.409 6 2 49908869 870168 5110AGCACTTCCCRTTTATGAGAG 0.555 0.545 0.391 0.268 0.929 6 2 4992127410174758 5111 CTACTGTGACWATATATTTAA 0.409 0.406 0.483 0.473 1.258 6 249933375 2350705 5112 TTCCCAAAGGSTAACGGTAAC 0.074 0.071 0.225 0.9481.170 6 2 49948039 7584378 5113 CTCTATGTCAMTGTCCGTCTA 0.492 0.494 0.2910.869 1.478 6 2 49958503 17795388 5114 GATTTTTTAARTCAAAAGAAA 0.176 0.1751.095 1.153 2.016 6 2 49969040 17039353 5115 GTTTCCCTGAYAGCCATGGCT 0.8050.803 1.106 1.064 2.682 6 2 49991254 10495984 5116 AATGATTTATSTGTTCCTATG2.244 2.279 1.707 2.081 2.920 6 2 49999544 17039373 5117TTTTCTACCAYACTTTTTTCA 0.041 0.040 1.682 2.662 2.829 6 2 5000440417490406 5118 GTTTATGTGAYTGTGACATTC 1.689 1.693 2.435 3.187 2.569 6 250010410 1981797 5119 AATTTTTCTCYTTAATATATC 0.213 0.212 2.258 3.2582.432 6 2 50037491 1156742 5120 AATGGTATAARAATATTAGGG 2.618 2.545 3.2752.886 2.518 6 2 50048374 17039425 5121 CTATTTGCCTRTGATTTTTAA 1.846 1.7993.303 2.512 2.671 6 2 50055972 12465974 5122 ATCAAAGATTYCAATTCTTGG 2.9623.009 3.364 2.444 2.665 6 2 50064931 10495987 5123 TTGGAAGCTAMTGATTATTCT1.835 1.901 2.362 2.249 2.575 6 2 50077180 6758043 5124ATCATTGCTTRTGTAATCTGA 0.548 0.539 1.855 2.738 2.420 6 2 5008330912618646 5125 TAGTTGCTTCRTTGCGCTTAC 0.738 0.723 1.215 2.305 2.425 6 250089802 1363046 5126 CTGTTTAAAAWGTATGCAATG 0.429 0.449 1.267 2.1132.252 6 2 50095750 6713560 5127 AGCTTTTAACRGTTCTCAAAG 1.039 1.073 1.4041.467 2.497 6 2 50101824 17039579 5128 ACAGGAAATAWCACGGATTCA 1.856 1.7601.294 1.021 2.086 7 2 50107806 17039592 5129 GGGTGCTTAGWAATTACTTAT 0.6630.680 1.326 1.111 1.801 7 2 50114259 10495989 5130 CCAAAGCTCARTTTGTCTTGG0.235 0.203 0.930 0.914 1.050 7 2 50127794 17439837 5131CCCAGCACAAYGTGGCAGCCA 0.316 0.334 0.321 0.854 0.613 7 2 50135085 18979245132 TGTAGAGAAGRTAGAAGAACA 0.251 0.259 0.181 0.605 0.628 7 2 501427621421577 5133 AGAGATACAAMATAATAATTC 0.557 0.563 0.216 0.165 0.463 7 250148980 10495992 5134 ACACCAAATAKTATTTCATGA 0.245 0.245 0.220 0.0740.498 7 2 50155053 6731061 5135 TTCAACATTAYTCACAGAAGG 0.266 0.276 0.1800.144 0.321 7 2 50161986 4971634 5136 TCTTCCTGGARAGAAAGTCCT 0.322 0.3270.074 0.097 0.109 7 2 50168831 17039678 5137 TGGGTAACTAYGTGTCAAGTG 0.1440.148 0.166 0.136 0.098 7 2 50174623 2117560 5138 AAAATACATTYATGTACCTTA0.076 0.073 0.099 0.075 0.095 7 2 50180362 10191989 5139CTCTTTGAAAYGGGCAAGCTA 0.637 0.609 0.135 0.090 0.093 7 2 5018771212470518 5140 AGTCAAAAACYAGAATCTCTC 0.002 0.002 0.121 0.152 0.090 7 250194688 17039734 5141 ATAGATCTAAMCTAAGTGAGA 0.588 0.577 0.213 0.1140.058 7 2 50201562 17039741 5142 TTCATTTTAGYTCTTCACTCT 0.172 0.177 0.2110.132 0.100 7 2 50208052 1563021 5143 CAAAGAAGGGYCACAGAAACC 0.383 0.3780.270 0.165 0.099 7 2 50215591 10490244 5144 AACACTATTAYGAGAATGCTT 0.6030.586 0.200 0.091 0.147 7 2 50225689 1563026 5145 TGTTCATTAGSTTTTACAGTG0.106 0.106 0.224 0.225 0.137 7 2 50233983 17039807 5146CTGATTTTACKTTTTATGTCT 0.269 0.270 0.151 0.150 0.203 7 2 50244051 93091765147 AGCATTAAATYAGTCCAGTTG 0.265 0.261 0.183 0.286 0.139 7 2 5025140612469244 5148 GATAGGATTCYATGAGGGTGC 0.030 0.030 0.150 0.222 0.168 7 250258695 896685 5149 AGTATTGTAARAAGTCTCTCT 0.681 0.659 0.315 0.208 0.1627 2 50265931 985132 5150 CCTCCATTCAYTAGGGAGATA 0.173 0.171 0.278 0.1920.200 7 2 50273114 10490240 5151 TTCCCTCACCRTATTTCTAAC 0.922 0.897 0.4750.172 0.249 7 2 50278945 17039985 5152 TGAAAGATTGYTTTGTCTCTT 0.097 0.0990.256 0.231 0.174 7 2 50285638 10490238 5153 GTCATACTTASGTCAGTCTTC 0.6240.604 0.283 0.313 0.256 7 2 50293510 17040013 5154 AGGTATTCTGYAGATATAATT0.068 0.067 0.163 0.319 0.266 7 2 50298927 6723207 5155AGAACTAGTAYATAGTGAGGG 0.221 0.254 0.260 0.321 0.248 7 2 5031207212994146 5156 ATGAAAGCAASTTAAGTCTCA 0.553 0.538 0.287 0.293 0.439 7 250318605 1377241 5157 GCTGCTTAGGRGTGCTTAACC 0.000 0.000 0.294 0.3440.315 7 2 50324810 17040123 5158 AGTTAAATACYGATTTATAAA 0.671 0.664 0.5210.217 0.318 7 2 50331889 4971658 5159 GCACAATAGTRTTGCATTCCT 0.067 0.0680.464 0.502 0.184 7 2 50337287 4971659 5160 CCCAACCTCTYGAATGCCCAT 0.7400.736 0.327 0.436 0.214 7 2 50344043 1712886 5161 TTATCATCTGMAAAATGAAAA0.412 0.411 0.484 0.333 0.176 7 2 50350726 1915221 5162GTACTCTACAYAAATCTTGCA 0.115 0.115 0.468 0.252 0.239 7 2 50353927 67241285163 ACTTCCTTTAYGTTTTCTTAT 1.139 1.112 0.255 0.182 0.314 7 2 5036340517509125 5164 AAATGGAGAAYCAATTTTACC 0.056 0.056 0.177 0.236 0.251 7 250375352 1618655 5165 CAGAATTAAGRGGAATTCACA 0.164 0.182 0.259 0.1980.209 7 2 50388807 633128 5166 TAAATAACTTKTCTAATAAAT 0.133 0.133 0.0610.266 0.141 7 2 50395288 2682005 5167 CTAAATTTTGRTACTTCAATA 0.397 0.3690.162 0.268 0.205 7 2 50402259 1712896 5168 GTAGCTATTARGTCTTCAAAC 0.3020.306 0.307 0.092 0.131 7 2 50405457 13030223 5169 TTAGTTGTCARTAACACTGGC0.462 0.474 0.310 0.098 0.107 7 2 50417935 7422036 5170AATACAAATARTGGTGTTAGT 0.690 0.720 0.232 0.162 0.166 7 2 50428271 — 5171ATGAATGTGTRGTATAAAGCT 0.125 0.124 0.171 0.155 0.113 7 2 50434710 75920855172 GTTTATTACASTAATTTCCCT 0.074 0.078 0.204 0.112 0.105 7 2 504420461712891 5173 ATTTTCACATWTCTATGCATG 0.139 0.138 0.058 0.173 0.094 7 250452128 10490229 5174 AATATTGCTCYTCTGAGTGCC 0.616 0.623 0.060 0.2120.112 7 2 50463718 — 5175 CTGGTTGTGASGAAGCTGTGC 0.116 0.117 0.201 0.0890.098 7 2 50479374 17040473 5176 AAATATCTCCMGTTACAATAT 0.142 0.133 0.3850.084 0.099 7 2 50492880 12475979 5177 TCCAATATAAYTACAGGCAGA 0.688 0.6820.191 0.118 0.084 7 2 50505813 4377361 5178 GTACGCCAGCYGAGGCCTCCA 0.6790.677 0.167 0.142 0.054 7 2 50513350 4971667 5179 TCCACTGAAGYTACTATTATC0.055 0.052 0.223 0.100 0.117 7 2 50519950 1402126 5180AAAGAGTTCARGTTGAAGAGA 0.026 0.026 0.107 0.124 0.141 7 2 5053070910191436 5181 TCTATGTTTCYGACAATAAAA 0.275 0.273 0.028 0.157 0.195 7 250537843 12713098 5182 ATGGATTTTCRAAAGTAAAAC 0.256 0.256 0.077 0.1840.252 7 2 50545936 13392422 5183 ATGGCCTCAAYATTCTACTCT 0.262 0.267 0.1370.104 0.252 7 2 50555070 1915170 5184 AATAAAATATRCCAAGAAAGG 0.345 0.3460.326 0.192 0.305 7 2 50564676 7423850 5185 GTCACTTCACYGGTATTCTTC 0.2180.225 0.329 0.410 0.225 7 2 50571943 2160444 5186 TGGAAAGATGWAAAGCTCCTT0.819 0.825 0.404 0.388 0.133 7 2 50578856 12613343 5187AAAGATTTACSGACACTTCAA 0.179 0.194 0.651 0.427 0.144 7 2 50584538 — 5188CAGCAAATGARCAAACGGTAT 0.514 0.510 0.618 0.394 0.302 7 2 50594113 10054315189 TTTACATGGCYATAGCTTTCA 1.024 1.002 0.465 0.315 0.336 7 2 5060870817040688 5190 GATTATGTGGRTAAACAACAC 0.194 0.181 0.440 0.282 0.338 7 250614171 17040693 5191 ATTGAGGCCAYGGTAGAAAAG 0.489 0.480 0.268 0.3670.571 7 2 50619827 10197380 5192 CTACAAGAGGRGTAAAGGTTA 0.185 0.181 0.0550.427 0.503 7 2 50627282 6726707 5193 TGGGCAGCTTYTGGGAGGCAG 0.037 0.0350.291 0.371 0.505 7 2 50634579 2241175 5194 TGCTGAAAATKAAATTCTGGT 0.1680.167 0.305 0.502 0.517 7 2 50640296 17476739 5195 ACAAAAAGATMATTTCACAGA1.132 1.149 0.345 0.456 0.544 7 2 50647858 1014428 5196ACCTTTACATKATTAATCTGC 0.473 0.472 0.962 0.382 0.471 7 2 5065526917536202 5197 GAAGCATAGTKGAGTCACCTT 0.284 0.274 0.942 0.599 0.336 7 250662806 — 5198 GGAAGAAACARTACTGGAAAT 1.328 1.157 0.499 0.717 0.320 7 250668252 17040792 5199 TCTTGTATGGRTAACACAAAC 0.027 0.027 0.694 0.7230.233 7 2 50675296 10175222 5200 TTTTGCCATAKGTAGTAGCGG 0.264 0.254 0.7570.460 0.285 7 2 50681836 11125316 5201 TTGCCAAACTSTATATATCAA 0.875 0.8530.187 0.361 0.281 7 2 50696829 17040825 5202 GTGGTATCTARCAACAGCGGC 0.3720.371 0.301 0.284 0.320 7 2 50710112 12713112 5203 CAGTAAGTCASGAGTTTGAAG0.157 0.151 0.241 0.079 0.299 7 2 50719478 11125320 5204TCAACACATTSTAGATATCTG 0.364 0.330 0.038 0.076 0.248 7 2 5072771310179269 5205 TGAGCTCATASAATAAATCCT 0.117 0.118 0.055 0.096 0.361 7 250735982 12713117 5206 TTGTCTTGAGRAGGGACATGG 0.018 0.017 0.033 0.1280.361 7 2 50751973 12476492 5207 AACTTCTGCCRAAAACATTAA 0.480 0.473 0.0380.109 0.403 7 2 50764421 17040906 5208 ATTGGGCACAMGTACAACTAT 0.011 0.0110.278 0.242 0.646 7 2 50772700 10176888 5209 AGGGAAGTTGWTTTAGATGAT 0.4170.423 0.340 0.554 0.645 7 2 50779781 13390911 5210 TCCTGAAACASTTTAAGAGAA1.037 1.024 0.498 0.596 0.858 7 2 50788335 12713120 5211CTTAGTGAGAYTGGTTGGTTT 0.158 0.157 1.162 1.082 0.893 7 2 50803660 8589285212 ACCTTCTTTGMAGAACAATAC 0.908 0.914 1.085 1.277 0.870 7 2 508106069309186 5213 ATATAATAGTYGTAACTGCAC 1.522 1.552 1.301 1.763 0.876 7 250821305 2194393 5214 TCGTGACTTGSTTGACACACA 0.268 0.268 1.662 1.7051.561 7 2 50831786 2352074 5215 TTATCAGTTASTGTGTCAATG 1.317 1.324 1.8931.331 1.423 7 2 50837738 10490221 5216 AAAAGTTCTCWCTATATATAC 0.805 0.8101.208 1.347 2.248 7 2 50844593 1518548 5217 TAATTCAGGARTTGCCCTTAT 1.1851.256 1.095 1.720 2.339 7 2 50851819 858936 5218 AAATTTAAAAYAGCTGAGTGT0.244 0.233 0.564 1.078 2.763 7 2 50858024 17040976 5219ACCTCTTACCRTAATTCCAGT 0.237 0.241 1.112 1.743 2.599 7 2 50866998 — 5220CTCATAATGTMCTTCTGCTAC 0.118 0.124 0.588 1.829 2.564 7 2 5087675810184889 5221 CAAAGACACARAGGTTTATCA 1.801 1.960 1.453 2.450 2.705 7 250883768 17502407 5222 GCTTCACATGYTGCCCTCTCA 0.284 0.293 2.128 2.2932.682 7 2 50896377 9309194 5223 CACACATGCARTATCTCCCTT 1.989 1.989 2.9772.177 2.399 7 2 50905547 9309197 5224 GGGAGGGTCARTTACCAGGAC 1.352 1.3572.243 2.498 2.297 7 2 50914257 3850332 5225 GATCTGGAAARTAACTATTAT 1.8281.766 1.974 2.630 1.780 7 2 50922546 3850335 5226 CTTTTCCAAGYGTGGGAATAA1.000 1.007 1.930 2.088 1.869 7 2 50933139 17568791 5227GGACATGTTAYGGCTGTGAAA 0.303 0.310 1.698 2.193 1.842 7 2 50940313 23520775228 ATGCGAGGACYAAAGCATTCT 1.215 1.239 1.162 1.471 1.914 7 2 509483639750635 5229 GCTATTAAAAYTCCTGAGATA 0.892 0.870 1.040 1.286 1.346 7 250959009 9309203 5230 GAACTTACTAYCCTTTCTATT 0.508 0.512 0.896 0.7681.319 7 2 50974365 — 5231 TGCCAGCACAYGACATTTGCT 0.715 0.702 0.558 0.5841.273 7 2 50985100 17504439 5232 CTCAAAGTGTYAGGTGTAAGG 0.042 0.042 0.2480.520 1.161 7 2 50992132 17504614 5233 GAGAAATAATYCTTCATGTCC 0.468 0.5000.217 0.269 1.618 7 2 50998498 6710741 5234 TACAGAGTACMCACAGTTCTC 0.1170.117 0.069 0.517 1.719 7 2 51013463 2193413 5235 TACTTCCAACRGGAGAATTCT0.448 0.438 0.111 0.578 1.985 7 2 51019436 983935 5236CAATTTGCACRAGGTTAACAG 0.090 0.085 0.593 1.624 2.003 7 2 5102539310490172 5237 TTGCTGCTTASAAACAAATTC 0.296 0.282 0.831 2.248 2.616 7 251032119 17041091 5238 GGACAGACAAKTATTTTGTAG 1.864 1.796 2.938 2.3293.723 7 2 51039440 17041095 5239 TAGGGCTCTTMAATAGTAGGA 0.679 0.695 4.0362.584 3.676 7 2 51046992 1160595 5240 TGTTTCATGTYTTAAAGTGGG 3.562 4.1534.036 3.775 4.225 7 2 51052993 1003017 5241 TGGACATACAMTAATGTATCT 1.3201.479 3.511 5.267 4.568 7 2 51058686 741421 5242 CTGTCATTCAKTCTAACTTGC0.900 0.851 3.988 5.267 4.568 7 2 51065534 7602202 5243AATATTTAGCYCAATTTCTCT 1.333 1.285 3.944 4.036 5.267 7 2 51076203 15416025244 CTATCAGTAGYATGGGAATGA 2.030 1.966 3.481 4.153 5.267 7 2 510838057579976 5245 CATTCTAACAYCTACAAAGAG 2.947 2.726 3.488 3.966 5.267 7 251091598 — 5246 ATCTATGCCASATTCCACTGA 0.074 0.074 2.730 3.966 3.869 7 251096961 10205578 5247 TGGAAAGATTKTAATCAGAAA 1.090 1.045 2.689 3.9663.988 7 2 51103966 12612704 5248 TATAGATATAYGATATATTGT 0.000 0.000 1.6093.225 3.422 7 2 51107252 10174398 5249 TCTGTACCCAYGCTCTCTCCA 1.535 1.4292.171 3.153 3.364 7 2 51117400 — 5250 GCATACAATGYTTGGCTAATG 0.945 0.9042.165 2.245 3.275 7 2 51126792 17041161 5251 AGAAAATCCAYAAGTCTAAAT 1.1101.128 2.105 2.396 3.031 7 2 51134758 888242 5252 TAAGTATTTAYATAGGTTATC0.873 0.894 1.629 2.107 2.694 7 2 51141925 17574007 5253TTCTATGCAGMCCTCATAAGG 0.931 0.932 1.641 1.847 1.807 7 2 5114832617041184 5254 TCAAGTTCCAYTATGCCTGCA 0.705 0.698 1.282 1.468 1.743 7 251158533 12474437 5255 TTTGTGCTCCRTGGATGCAAG 1.386 1.326 0.973 1.2541.738 7 2 51174609 — 5256 ATGGAAATGCMTGCTCACCTA 0.285 0.277 0.894 1.2131.657 7 2 51181930 — 5257 TCCTAGGAGTRTAGGATGAGT 0.209 0.217 0.776 0.9781.281 7 2 51195568 6545191 5258 TTTAGAGTTTWTTGTTTTATG 0.893 0.816 0.7161.101 1.061 7 2 51207597 — 5259 AGTACACACASTGACATAATC 0.408 0.402 0.5940.995 0.884 7 2 51219646 6545192 5260 AATGGGCTAASTTTCTACTAC 1.232 1.2391.068 0.588 0.849 7 2 51226179 17041243 5261 GAAATCTAAGKTTCTCTTTTA 0.0250.025 0.900 0.552 0.672 7 2 51232787 1549704 5262 CAGCTGGAATKCACAACGAGA1.400 1.371 0.728 0.556 0.560 7 2 51239401 12618610 5263TTTCTATTAGMCCCTTAAAAA 0.303 0.299 0.240 0.490 0.356 7 2 51246851 — 5264AGGACAAGCTYGTTGGAAGTA 0.025 0.025 0.345 0.436 0.533 7 2 51257125 49715695265 AGGTGCTTTCMTGGGAGTCCC 0.120 0.122 0.107 0.168 0.618 7 2 512675621013164 5266 CACTGGGACTRGGTATGGATC 0.258 0.245 0.087 0.263 0.435 7 251280190 10186486 5267 CCATTTCCCTRGAGATTAAAT 0.634 0.606 0.130 0.2160.373 7 2 51286897 — 5268 GTTATGTATGYGTGTACATGT 0.260 0.257 0.216 0.2710.246 7 2 51292648 13404753 5269 GCTAGGAGGARTTAAAATAGC 0.177 0.180 0.5230.276 0.374 7 2 51298389 13428293 5270 CAGTTTGCTCKGCTAAGAGGT 0.441 0.4310.522 0.272 0.170 7 2 51306221 880384 5271 AGAGGGCCAARGCTTTTTTTC 1.1411.087 0.430 0.336 0.171 7 2 51312996 17518964 5272 TTAAATGATTKCAGGTCTTTA0.589 0.597 0.392 0.372 0.232 7 2 51313386 17578308 5273GAAGTTGCTAYGTAGTGTGGC 0.014 0.013 0.423 0.339 0.314 7 2 51338825 37320515274 ACTTAATGCAYCAAAGTAATA 0.139 0.130 0.284 0.367 0.431 7 2 5134463212477826 5275 TCTATAATTAMCTCTTGCTGA 0.484 0.478 0.133 0.378 0.363 7 251350968 1468896 5276 TGATCTTTGAKGTTCAAAAAA 0.710 0.755 0.220 0.2340.374 7 2 51356868 17041444 5277 TATGCCATCTRCAATGACTGG 0.110 0.113 0.3340.325 0.539 7 2 51362562 6745538 5278 AATAATTGGTYCCCTTGTGAC 0.329 0.3220.407 0.382 0.497 7 2 51378902 2041947 5279 CTGTATAAACRTATGAAATTA 0.0000.000 0.473 0.437 0.264 7 2 51389580 7583309 5280 ATTTAAAATGRTATTACCACT0.610 0.600 0.527 0.591 0.237 7 2 51403035 13431262 5281TACATAGTTAYGCAAACACTA 0.883 0.870 0.519 0.405 0.248 7 2 51408733 67218295282 TTGCAAGCCARGAGGTTCTAG 0.222 0.239 0.742 0.395 0.328 7 2 5141472312470385 5283 CAACCCTTCASTAATTCCAAA 0.341 0.336 0.541 0.425 0.708 8 2121573870 12477717 5284 AGCCCTGGAGKGTTGGTCTGA 1.145 1.120 1.004 0.8511.428 8 2 121575378 17005869 5285 TGTGGGGCTAYGGGCCAGGAA 1.463 1.4961.163 0.989 1.320 8 2 121590453 17005870 5286 GAAGCAAATAYGGTAAAATTT0.443 0.447 1.707 0.979 1.575 8 2 121599976 10169502 5287AAACGGGAATRATGGTCAGAC 0.276 0.269 1.085 1.693 1.341 8 2 12161245612475346 5288 TGCAGATCACYGAATTTCCCC 1.048 1.010 0.486 1.687 1.418 8 2121618246 — 5289 ACCGTGCAGCYACAGCTCAAA 0.348 0.340 0.975 1.686 1.451 8 2121626028 6724707 5290 ATGCTCTTTTYGTGCACCTTT 0.420 0.409 1.100 1.2831.644 8 2 121627437 12616919 5291 TTGGACTGTCYGGTTATTGTC 1.475 1.4231.237 1.148 1.721 8 2 121633564 10189645 5292 GCTAATGTAAYTTTGGCCACA0.657 0.658 1.452 1.205 1.278 8 2 121646388 13015322 5293CAGGTAAGAAYAAGAAAACCA 1.479 1.528 1.452 1.060 0.816 8 2 121654594 — 5294GAAACAGCATYATCACATACC 0.000 0.000 0.873 1.224 0.814 8 2 12166402017179427 5295 GAGCCTTGTGMATGGGCCGGG 0.191 0.185 0.783 1.071 0.890 8 2121674800 17006162 5296 AAACACAGGAYCTCTTTTCTT 0.327 0.322 0.342 0.5180.845 8 2 121684644 4073806 5297 GTCACCATATRGCAGCTTGCC 0.000 0.000 0.1780.458 0.838 8 2 121692872 17794420 5298 CATGCAACCCRAGCGGAAGTG 0.7280.697 0.182 0.160 0.877 8 2 121700701 12473888 5299CAAGTCATGARCCCTGGACAA 0.027 0.027 0.206 0.248 0.526 8 2 1217102853768905 5300 CCCCGAGAGCYTGGTCCCACA 0.143 0.147 0.241 0.279 1.162 8 2121723608 2119021 5301 AGCTCAGCTCMCTCTCTGGAC 0.000 0.000 0.231 0.3141.198 8 2 121734993 17006292 5302 GCCTGATGGAMTATCACCTGC 0.474 0.4760.360 0.299 1.342 8 2 121746144 11122852 5303 GCTGAGGTTCWCGCCTACCAG0.768 0.767 0.547 1.036 1.462 8 2 121753101 2953083 5304AGTTGGTAGCMCTTGACCTTC 0.291 0.314 0.491 1.859 1.421 8 2 121768706 — 5305ATGCACTGCASCAGCTCTCCA 0.000 0.000 1.621 2.055 1.765 8 2 12177531413028716 5306 TGCCTTTCCAYCTGGACTTAT 0.380 0.381 2.154 1.930 2.827 8 2121794928 2580373 5307 AACTGTGCCAYCTCATTGTCC 2.612 2.642 2.390 1.7462.636 8 2 121820468 12622908 5308 CTGTTGCCACYACTGATGCCC 1.503 1.5742.247 2.043 2.476 8 2 121833968 12479320 5309 TGGTGCCAATRTCTCTAAAAG0.910 0.883 1.897 3.093 2.476 8 2 121834342 17006394 5310CCTAAAAAATRGCAAGAAGGA 0.385 0.395 1.392 2.712 2.449 8 2 1218597956716602 5311 CAGAGGGAAAYTTGAGGGAGT 0.275 0.270 2.539 2.510 2.203 8 2121870433 10496566 5312 ATATAGAATAWGTTTCAGCCC 1.420 1.483 2.237 2.0102.204 8 2 121886006 10191223 5313 ACCACAGAAAKGGAGAAAAAT 4.004 4.1532.200 1.819 2.021 8 2 121891735 10496567 5314 CTACGTCTAAKTATTATAAAA0.206 0.207 2.677 1.574 2.096 8 2 121897370 10496568 5315AACAGACATCMTATAAACTTA 0.394 0.401 2.153 1.799 1.718 8 2 121911303 7565045316 TTGTTTGTGTSCTGTGGACAC 0.000 0.000 0.183 1.718 1.730 8 2 12192378517006485 5317 TATCAATGCAYTGCCCAAGAG 0.438 0.414 0.421 1.667 1.631 8 2121930788 17006497 5318 ACGACTTGCTRGAAAGAGGGT 0.228 0.224 0.324 0.5932.019 8 2 121936188 2118387 5319 TTGCTCCAACRTTTTCTGGTC 0.761 0.773 0.8231.071 2.097 8 2 121950085 17039719 5320 CTCCACTGAASTCCCTAAGAA 0.1620.164 0.790 1.212 1.815 8 2 121958256 17006551 5321ACATCCCATGSTCCCCTTTGT 1.661 1.585 1.488 1.531 0.947 8 2 121964620 — 5322GGAAACACTTYCCTAAGATAC 0.366 0.318 1.551 1.391 0.909 8 2 1219899996730694 5323 GTACAGGCCAYATGAAGTCCC 1.813 1.761 2.134 1.540 0.873 8 2121994411 7608301 5324 ACCAAACTCARCAAGAGGGCC 0.752 0.737 1.549 1.2910.795 8 2 122017375 2164797 5325 AGCCCCCAGCSCGCCAACAGG 1.546 1.546 1.6901.212 0.740 8 2 122041926 4848712 5326 TTCATCCACTYGTCTAGAGGG 0.026 0.0270.693 0.767 0.752 8 2 122042281 — 5327 AATGAAACAARCATTTCTTTA 0.593 0.5910.420 0.733 0.648 8 2 122065350 10496574 5328 CAGTACTAAAKTCCTATACTA0.062 0.067 0.037 0.299 0.628 8 2 122070442 10496573 5329AGTCTACCAARGAAGTGCTAA 0.103 0.103 0.068 0.192 0.321 8 2 122093087 2877905330 TCTTATCTTTRTGCTTTCCTG 0.139 0.143 0.018 0.020 0.315 8 2 12210255810185512 5331 TAAAAAGTAGYAATACATAGG 0.224 0.230 0.033 0.024 0.138 8 2122108669 192608 5332 CTTCTGTCAARCTTTTTAGAC 0.165 0.168 0.036 0.0040.125 8 2 122119204 6541792 5333 TGCCTGCAGTSTGACACTGAT 0.241 0.249 0.0350.009 0.051 8 2 122132877 — 5334 CAGCAAAACTWTTTATACATC 0.214 0.215 0.0170.025 0.073 8 2 122138380 6541794 5335 TTTCTTATCARTGGGCCCTCT 0.093 0.0960.035 0.061 0.122 8 2 122165738 17833009 5336 AGAATTCATAYACAAACCCCA0.037 0.037 0.078 0.138 0.159 8 2 122184058 17039745 5337ATAATTTCCTYCTTTCCAATG 0.277 0.274 0.179 0.177 0.251 8 2 122191938 — 5338ATAGTGGTAGWTTCTGTCCTG 0.512 0.507 0.372 0.392 0.326 8 2 1222061626726677 5339 TCACATATTCYGGGGACTGTT 0.581 0.573 0.546 0.471 0.390 8 2122224032 287803 5340 AGTGCTAACASGTCAATAATA 0.773 0.771 0.998 0.6610.481 8 2 122232697 — 5341 GCTTACAACTMGGCAAACATC 0.000 0.000 1.059 0.8460.559 8 2 122238287 7565841 5342 CGTATGTTGASAGTGCACTAA 1.103 1.112 1.0480.949 0.547 8 2 122263080 17006811 5343 AAGATTGAAARTCACTTATGA 0.0000.000 0.973 0.935 0.563 8 2 122273164 4848159 5344 GCATGAGCATYTTTCATTGTC0.760 0.746 0.955 0.982 0.653 8 2 122285711 4848719 5345CCATGGCCTTRGCCTGTCTGC 0.564 0.573 0.694 0.794 0.572 8 2 1223113456761861 5346 AACGATGACCSAGAGGCTCTT 0.559 0.553 0.720 0.669 0.687 8 2122315106 6541810 5347 CACACAGGCTSTTTAAGGTAA 0.565 0.587 0.484 0.4280.562 8 2 122336423 4430963 5348 GGCTGAGTAAMAATCTTTTTG 0.664 0.591 0.3140.329 0.421 8 2 122352090 4254513 5349 CAGAATTCTARGTTGGTGGTG 0.135 0.1400.267 0.393 0.612 9 2 127494872 17014773 5350 GAATTCTTTGSTGTAAAGCTT0.488 0.454 0.754 0.234 0.962 9 2 127507108 11893989 5351GTATTTGATCRAATCTCTCAT 1.817 1.824 0.552 0.385 1.199 9 2 12751388611902275 5352 GGACCACAGCMTGGCAGAGGA 0.248 0.246 0.616 0.383 0.781 9 2127526927 17014818 5353 GACCGGCCCAYTTCTGTCTGG 0.106 0.108 0.701 0.7440.754 9 2 127536416 17014851 5354 CAGGCACGAGMGGAGGTTTAT 0.162 0.1600.073 1.289 1.256 9 2 127542763 17844995 5355 TCTTTTTGGCRGTTTGAAGGG0.628 0.659 0.592 1.379 1.247 9 2 127549063 6754017 5356TTGCATAGATSTCTGTCAAAC 0.019 0.019 1.459 0.841 1.058 9 2 12755327113430599 5357 CCTCACACCGKGTCCAGGCTG 1.786 1.860 1.642 1.390 1.355 9 2127559423 13425613 5358 GAGGAAGGAGMCCGAAAGAAA 1.569 1.611 1.341 1.4021.332 9 2 127594668 4663100 5359 TTCCCGTTTGSGGTTGGGGAC 0.645 0.637 2.1901.338 0.835 9 2 127606162 6710467 5360 ATCCGAAGGTRAGTGGTATTT 0.132 0.1361.258 1.478 0.848 9 2 127613550 3943703 5361 CATTATTTTAYTTTCCCTTAT 1.5561.582 0.493 1.680 0.842 9 2 127620718 10208217 5362CTAATACTTASGTGTACATCA 0.087 0.083 0.630 0.896 1.364 9 2 127630065 7296665363 TCACTCTGCTYTTTGGAATGT 0.064 0.065 0.742 0.454 1.745 9 2 12763846217701884 5364 TGCTAAACATRAGATACTCAG 0.931 0.962 0.186 0.283 2.625 9 2127644972 17015008 5365 AGCTGAAAGGRCTCTGAATGT 0.381 0.386 0.282 0.7323.364 9 2 127650421 4662708 5366 AGTCGGCAGAKGCTCTGAGAT 0.161 0.164 0.2530.798 3.735 9 2 127668493 13006847 5367 GCTGAACAGGYCAAACTATTT 0.2770.260 0.494 1.511 3.551 9 2 127692604 6710496 5368 AAGCCCCCATMCCAGAAATAA0.050 0.068 1.208 3.267 3.775 9 2 127723916 6430936 5369CCCGAACAGGMTTTTGTTCAC 1.597 1.809 2.784 4.187 3.403 9 2 1277297344662717 5370 GTTCCTGGGGYTTGCACTGAT 1.757 1.935 4.187 4.062 3.488 9 2127749557 4150474 5371 ACAAACCCACMAAGAAAACAG 2.034 2.101 4.789 4.3123.568 9 2 127754774 4150454 5372 CCATCACTTTYAGACCTGTCC 4.078 4.488 4.3124.153 3.735 9 2 127776393 4233584 5373 CCTGGATTTCYTACTCACTGT 4.250 3.8044.568 4.036 3.735 9 2 127802584 12613413 5374 TTTTGATGGTYCACATGCCAA0.498 0.536 4.153 3.775 3.761 9 2 127810283 17015199 5375TTTTCTTTCTRCTTCCCAAAG 2.959 2.899 2.959 3.723 3.698 9 2 1278284316714840 5376 GCAAAGGATCWGTTTCCAAGT 1.018 1.018 1.933 3.835 3.944 9 2127831525 6727155 5377 TTTAAAGGGCWAAGGCTATTC 0.731 0.715 1.960 2.9723.467 9 2 127862992 2276683 5378 AAACACAGAGSTCAGTGGAAA 0.386 0.393 1.1601.860 2.951 9 2 127880407 7599210 5379 TCCTTGGCCARGCATACTGGC 0.000 0.0000.823 1.794 2.448 9 2 127886129 6753288 5380 ATTATAGCGCRCTTAAGTTAC 1.3101.269 0.745 0.722 1.996 9 2 127906285 1568277 5381 AAGAGAAACAYGGATGGAGCG0.247 0.251 0.750 0.526 1.315 9 2 127912750 12478656 5382TCATGGGCAGKAAACTGTTCT 0.587 0.586 0.536 0.377 1.299 9 2 12793046317015274 5383 GTGCTTAGATRTGGAGAGCAT 0.377 0.384 0.221 0.603 0.723 9 2127937786 3889307 5384 CGGTCTCAACYTGGTTTTGTT 0.017 0.017 0.165 0.7200.642 9 2 127945128 10496663 5385 ATGACAAGTGYTATATGTTAG 0.299 0.3040.399 0.442 0.748 9 2 127955157 777557 5386 TTAGAAACGCRTATTAAACTC 0.1120.110 0.581 0.433 0.676 9 2 127963870 334156 5387 CCTTGAAGAGYCCATTATTTC1.292 1.359 0.662 0.441 0.682 9 2 127971045 11681225 5388CCCGGAACTTKTCATGCACTA 0.932 0.970 0.652 0.677 0.475 9 2 127982848 — 5389CACGAAAACAYAGCATTATGT 0.209 0.218 0.953 0.705 0.589 9 2 127988904 3341375390 CAGTTTAATCRCTCATTTTCT 0.292 0.285 0.917 0.794 1.068 9 2 12800634411684705 5391 TTCAAGGTTASCTGAATATTT 0.633 0.642 0.458 0.873 1.011 9 2128015247 2052954 5392 GTGAACGTCAYTACAAAGTGC 1.193 1.233 0.587 0.6881.310 9 2 128023821 17015401 5393 ATAGCTCCTGKTACTTTTGGC 0.030 0.0310.581 1.356 1.335 9 2 128033130 11688201 5394 GCTTAAAGTTRTGTCTATAGC0.536 0.569 0.678 1.209 1.618 9 2 128040262 — 5395 ATCCCATCTTYCCAGAGGAGA0.258 0.258 1.269 1.394 1.181 9 2 128057833 10928772 5396ATGCAGCTGTKTGCAGATGGC 0.861 0.822 1.262 1.237 0.907 10 2 16206773310193429 5397 TGTGGAAATAYTGTTGTAAAA 0.218 0.222 0.178 0.176 0.084 10 2162078126 12463483 5398 TTTCAAGCTGKCCAACACCCA 0.292 0.292 0.172 0.1660.079 10 2 162084212 2216941 5399 AGTCTTTCCCWTTCAAATCAA 0.138 0.1230.248 0.178 0.126 10 2 162090777 16845844 5400 TCTGGTCATCRTTATTTTTGG0.211 0.218 0.233 0.190 0.091 10 2 162114653 12470386 5401TTACTCTTCAYCTAAGTTTAG 0.807 1.157 0.159 0.088 0.105 10 2 162122423918964 5402 ATTGATTTCCRTGAGCTGAGC 0.182 0.178 0.244 0.145 0.203 10 2162130415 963758 5403 ATTTGGAGCAYTTGCCAGAGC 0.057 0.056 0.205 0.1310.424 10 2 162147459 6742389 5404 ATATTTTGTAYGATTCTAATT 0.000 0.0000.110 0.182 0.387 10 2 162156767 994383 5405 ATCCTAATCCRTCATCTTCTA 0.0890.089 0.111 0.315 0.681 10 2 162174354 6731620 5406TAACTTTCTGYTGAAATGAAA 0.625 0.591 0.194 0.489 0.622 10 2 1621764113849351 5407 CAGAATTACCRTAAGACAGGC 0.166 0.169 0.334 0.543 0.713 10 2162200351 3914093 5408 CATTAATTTCRGAGAAATTGT 0.000 0.000 1.042 1.0950.664 10 2 162237504 13410944 5409 ACTTTTAGGTYTCAGGAAATT 0.779 0.7800.924 0.900 0.553 10 2 162264719 — 5410 AGCAGACTAASAAAAATTTAT 1.5711.539 1.761 1.093 0.640 10 2 162270121 971027 5411 ACACTTAGACWGATTCCATGT0.360 0.368 1.391 0.910 0.614 10 2 162283074 16845940 5412AGGTTTGACARATGCTGGCTT 1.720 1.820 1.354 1.114 0.547 10 2 162298795 —5413 GTCAGAGTTTRTTGGAATTTT 0.015 0.015 0.559 1.120 0.593 10 2 162310868— 5414 TATTAATATTRTGTTTATGAT 0.000 0.000 0.644 0.762 0.575 10 2162330250 10803781 5415 GTGAAATGGCRGCATTCTCTT 0.005 0.005 0.043 0.3390.545 10 2 162343872 2389424 5416 ATTTTACTATMAATGAATATT 0.000 0.0000.042 0.293 0.615 10 2 162354105 — 5417 ACGTAGGGTASATGGTGGGAT 0.2790.555 0.038 0.032 0.845 10 2 162365216 9677118 5418CAGCTTGAGTWATAGTTTGAA 0.004 0.004 0.114 0.043 0.625 10 2 1623746061567981 5419 TTGAGTGTTARCGTTGCTAAT 0.181 0.183 0.137 0.115 0.575 10 2162383186 7578900 5420 AATTGGTAGTSTGCCTCCATA 0.284 0.280 0.061 0.6060.224 10 2 162393175 17728078 5421 TAAACTGTGARTCAGTAAAAT 0.461 0.4570.248 0.584 0.227 10 2 162407761 4664048 5422 AATTTTCCTAWTGTATTGGCT0.050 0.051 1.019 0.473 0.248 10 2 162412390 16846076 5423ATTTTGGTTTYATTTGTCCAT 0.845 0.843 1.052 0.475 0.321 10 2 162434172971282 5424 AAAAAGGAAGYACATCTTGTG 1.929 1.967 0.899 0.450 0.321 10 2162444883 7609393 5425 CACTTAAAAAMCTAGCTCTTA 0.460 0.448 0.901 0.5190.240 10 2 162454850 10193728 5426 AAGATGCTATYGAGGGTTCTG 0.185 0.1810.556 0.517 0.243 10 2 162498178 7608076 5427 AGTGAGAATAYATGACATTTC0.000 0.000 0.099 0.653 0.312 10 2 162512676 1710513 5428ATAAAATTTTWAAAAAAAGCC 0.000 0.000 0.094 0.403 0.328 10 2 1625256801227925 5429 ATCTAAAAACRCTAATTTTTG 0.534 0.523 0.133 0.036 0.301 10 2162536523 1227936 5430 AACAGGCCCCWAATTATGCTA 0.446 0.419 0.151 0.0550.317 10 2 162542388 1227932 5431 AATTAAAAATMATAAACATAT 0.000 0.0000.126 0.075 0.175 10 2 162550936 10179404 5432 ACAGAAATAAYTGAACATTGC0.044 0.043 0.140 0.122 0.020 10 2 162557433 11683174 5433AAAAAGGTCTYTATTCATCTT 0.022 0.024 0.107 0.118 0.059 10 2 1625632216707956 5434 ATAGTAGAAAKGTCAGAAAAT 0.632 0.590 0.108 0.046 0.240 10 2162568431 16846177 5435 TGTTGGTTCARTCTGAATCTT 0.327 0.319 0.112 0.0230.631 10 2 162578850 17729070 5436 ACTAATCAAASAGTGCCATAT 0.265 0.2730.106 0.108 0.978 10 2 162586481 7594396 5437 TTGTCTCACARGAGGTCCATT0.131 0.133 0.023 0.493 1.313 10 2 162605465 6432705 5438ACAATCTCTAYTTACTTATGT 0.077 0.073 0.197 1.106 1.720 10 2 16261083010930036 5439 ATATGCCATGYGTATAGCAAC 0.035 0.036 0.760 1.332 2.008 10 2162628500 12474587 5440 GTTCATATTTKTCTCCAAGAT 1.212 1.159 1.358 1.6652.106 10 2 162636693 4637136 5441 TTTGGTTTACRTTAAATCAAG 1.717 1.7111.747 2.129 2.277 10 2 162644128 4500960 5442 TCCAGCAGCAYGTTACTGTCT1.911 1.835 2.098 2.442 2.361 10 2 162653508 4664442 5443CTTTTAATGGRTCCTATGTAA 1.537 1.515 2.562 2.350 2.299 10 2 1626572634295021 5444 CCTGTTGATTKTTTAGCTGAA 2.045 2.188 3.102 2.531 2.369 10 2162671072 1861979 5445 TTAAAGCCTGYAAGCACCAAA 2.474 2.222 2.983 2.7952.451 10 2 162680415 2287509 5446 CCTCCTTGTTKCTCTCCAAAT 2.232 2.2642.916 2.905 2.655 10 2 162688346 — 5447 CTTTTGGAGAYCCCTAACAAT 0.3940.395 2.740 2.784 2.683 10 2 162694710 1014445 5448TCTGTAAAGCRCTCTCATTTC 1.404 1.396 1.830 2.596 2.755 10 2 16270253512692646 5449 GATTTCCACTWCAAGTTGGTT 1.488 1.513 1.425 2.543 2.914 10 2162712470 2284873 5450 GGATATGGCCYAACAGGAAAA 0.038 0.039 1.570 2.0232.779 10 2 162726834 16822665 5451 TTGATGGAATYTTTACGATCT 1.562 1.5261.662 1.446 2.399 10 2 162741920 10490422 5452 CATTCTTATGKTGTTCTTCTA0.779 0.793 1.227 1.574 2.380 10 2 162749424 12469968 5453CTCTACCTCARTTATACATCC 1.477 1.503 1.267 1.178 1.731 10 2 16276497410930042 5454 TATCTTCACCRTCTGTTTCTG 0.154 0.142 0.834 0.735 1.187 10 2162777856 4420712 5455 GGAAAGATTAKATTTGAGCCA 0.253 0.222 0.519 1.2821.080 10 2 162789104 16846291 5456 AGGCACTTCAKTTCAAAAATC 0.660 0.6210.105 0.723 1.083 10 2 162797892 10185151 5457 CCTGTCTCTARGTCATGCATT0.066 0.068 0.549 0.475 0.720 10 2 162807953 10490423 5458GCACATGAATYGATATAAAGA 0.238 0.235 0.483 0.129 0.849 10 2 1628248956732914 5459 CAGACAATGGYAAAGAAGCTT 1.503 1.538 0.272 0.395 0.648 10 2162835518 13001107 5460 CTACTTGAAGSCCACAGAGAT 0.054 0.052 0.246 0.3560.729 10 2 162839680 4664450 5461 TATGCCCAGTKTTCTGGTGAA 0.032 0.0330.675 0.370 0.445 10 2 162857841 6707966 5462 TAAGGTTGCAYTGCCTCGTGA0.028 0.027 0.157 0.536 0.461 10 2 162869862 6715552 5463TTTTCCTGAARATCATCATTA 1.359 1.378 0.349 0.815 0.424 10 2 1628802314664452 5464 AAAGGTTCATYTTCTATCCCC 0.071 0.068 0.580 0.449 0.333 10 2162888686 6706926 5465 GAAGCACTAGKTTCCTTTGAT 0.645 0.639 1.122 0.4930.360 10 2 162898589 16846387 5466 TTTATACTTTYCTTGTTTTGT 0.735 0.6880.614 0.478 0.332 10 2 162906489 16846391 5467 ATACCTTTGARTAATCACTTT1.196 1.201 0.696 0.497 0.183 10 2 162911936 3788970 5468GCCATAATGARAATAATCCTC 0.207 0.198 0.444 0.244 0.188 10 2 162920558 —5469 CAAACTTGATKTCTTTATCTT 0.256 0.251 0.238 0.230 0.299 10 2 16292873516846429 5470 CCAGTGTTACYACCTTACATT 0.020 0.020 0.018 0.196 0.382 10 2162934353 1125605 5471 CATATCGTCAYATCATACTGT 0.095 0.095 0.008 0.1010.171 10 2 162940042 16846499 5472 TTTTTTTATCWACTATGCAAA 0.205 0.2040.031 0.048 0.221 10 2 162946354 6755575 5473 TTGTGAACTGYACTAATTAAT0.075 0.077 0.063 0.081 0.145 10 2 162954331 3747517 5474AACTGTCTCAYGTTCGATAAC 0.546 0.536 0.199 0.055 0.188 10 2 1629601894664053 5475 TAAACATGGGWTAAACTAAGT 0.114 0.131 0.312 0.119 0.063 10 2162966835 10179671 5476 GACAAGATCTRCATTTGATTT 0.778 0.746 0.288 0.1150.113 10 2 162973735 6758639 5477 TGCAAATTCTRTAATACTTTT 0.567 0.5740.278 0.274 0.296 10 2 162980587 2163215 5478 TAATCCTCACKCCTGGAAGGT0.012 0.012 0.276 0.275 0.368 10 2 162989298 12478709 5479CTGACCGTCAYGTTTAGGAAG 0.510 0.495 0.357 0.342 0.688 10 2 16300349112468353 5480 AGTTTAGTGARAAACCCTGGA 0.107 0.104 0.188 0.761 0.665 11 2198533144 3851979 5481 TTTGCTAGGCWGTAAGGAATA 3.455 3.551 3.241 2.2101.583 11 2 198538737 6707521 5482 TGAAGACAAGRACTAGGCCGA 0.000 0.0002.149 1.954 1.124 11 2 198546560 700686 5483 TAGAAGTCGARATAAGTTAGA 0.0820.083 2.141 2.240 1.112 11 2 198554006 700692 5484 CTTGAAGTCAYCCCGTGTATA0.227 0.224 0.243 1.372 1.235 11 2 198561218 — 5485TTTTCTGGATRTTAAACTACA 1.052 1.043 0.217 1.087 1.303 11 2 1985698309288280 5486 AACCCTGAGGRAAATTTAATA 0.015 0.015 0.300 0.138 1.397 11 2198577143 2060488 5487 CCTTAGGTTTKTATATTCTGT 0.275 0.286 0.387 0.1270.886 11 2 198585479 6734781 5488 TCCTCCATTCRTCTCCATTTC 0.296 0.3010.040 0.171 0.739 11 2 198593672 6760891 5489 CAAAAATCTAKGTATCTTCCT0.000 0.000 0.079 0.199 0.581 11 2 198602052 4241195 5490CTTTTGTCCCRAGTTCCAGTT 0.006 0.006 0.076 0.052 1.012 11 2 1986178261401092 5491 ATTACATAAGMCAACTCACAG 0.257 0.274 0.080 0.217 1.437 11 2198624136 1464211 5492 CAGTATTTAARTTACTGAGCT 0.000 0.000 0.062 0.8151.458 11 2 198631344 11887138 5493 AAACTAGACAWCTATACCAAT 0.261 0.2730.373 1.782 1.657 11 2 198644132 — 5494 TCCTGAGTAGWCACCAATGCC 0.1690.175 1.407 2.237 2.230 11 2 198651174 10497809 5495TTGGCAAATARTATTCGATTA 0.892 0.891 2.530 2.343 2.628 11 2 19867112916826320 5496 TTTGCAGTTAYAATGGAGCTG 2.505 2.450 3.024 2.977 2.794 11 2198678142 2139050 5497 CTTATGTTGGRGAAGTTGATT 3.055 2.742 3.221 3.1982.533 11 2 198684689 1518369 5498 AAAGTCATCTRAGTCAGAAAT 2.168 1.9943.271 3.221 2.605 11 2 198693707 1976772 5499 CTGGACTCTTKTATTGGTTCG0.574 0.599 3.156 3.369 2.563 11 2 198704803 1440090 5500TTTTGTTGTTKATGTTATCTT 2.221 2.000 3.071 3.071 2.388 11 2 1987071741016883 5501 CTCAAAGTTARAGACCATGAA 2.439 2.314 2.462 2.749 2.417 11 2198719433 16826740 5502 GGCTTTTCTGRTGATTCTTAC 2.259 2.181 2.482 2.2602.592 11 2 198730776 892514 5503 TTCTCCTTGGYGGGTGGAATT 0.006 0.009 1.6471.746 2.607 11 2 198736751 10497812 5504 CTTTATAATCRAGGGCTAAAT 0.4260.447 1.145 1.843 2.226 11 2 198746066 13382697 5505TAGGGATGAAMTAATCACTGT 0.137 0.141 0.576 1.308 1.866 11 2 1987552226434954 5506 GAGGGGCAGCYAAGATTTATG 1.326 1.355 0.577 0.916 1.462 11 2198765757 7590828 5507 CTCATAACTGYGTACGAAGCA 0.689 0.706 0.519 0.3481.729 11 2 198773023 16827521 5508 TAGGAAGGACRATAGGTGAGA 0.024 0.0230.772 0.474 1.363 11 2 198785109 3755333 5509 TGCCTATCTARTATTTTGTGC0.286 0.290 0.238 0.389 0.792 11 2 198791257 7570678 5510TACATTTATTYGACAGCTACT 0.755 0.786 0.175 0.671 0.347 11 2 198798289 —5511 ATCTTTTTCTRTACGATTCTT 0.057 0.058 0.211 0.460 0.417 11 2 19880802716827729 5512 AAGCTTCTTARCAATGTATAT 0.494 0.515 0.545 0.297 0.352 11 2198814784 16827788 5513 TAGTCCTGGGWAATTGGGACC 0.134 0.135 0.501 0.3210.521 11 2 198821526 16827819 5514 TTCATGGACASCTCACCTTCT 1.115 1.0640.526 0.353 0.264 11 2 198834891 16827923 5515 GAAAATGGCCMAGATAAAAAT0.695 0.703 0.363 0.201 0.187 11 2 198843361 11896429 5516ATTGAGGCACWACTGAAAAAG 0.112 0.113 0.452 0.428 0.259 11 2 19885047613031577 5517 TAAAAGTTATRTTGACTGGCT 0.124 0.124 0.143 0.316 0.232 11 2198863571 1368990 5518 ACACTAACTTYTTTGCTCTCA 0.393 0.395 0.229 0.3230.267 11 2 198873644 1434293 5519 GTGGCCATGCMTGAGGTTGAG 0.155 0.1500.219 0.198 0.270 11 2 198882620 3937566 5520 GTTTGTATGGYCTATTGCATA0.991 0.984 0.243 0.101 0.264 11 2 198899396 4850447 5521CCATTTATAGYGTTCCATGTG 0.045 0.045 0.274 0.245 0.281 11 2 1989053121598660 5522 TTCACTCTGAYTAATGGATGG 0.162 0.164 0.233 0.239 0.142 11 2198912596 6746027 5523 CAAGGTTTCAYTTGAGGTGCC 0.516 0.504 0.237 0.2560.080 11 2 198918962 13411216 5524 ATGACTGGTAYACTTTACACA 0.132 0.1330.239 0.267 0.078 11 2 198924942 12693836 5525 AACAGGAGAAYGGAATCCCTG1.012 0.999 0.364 0.096 0.098 11 2 198934154 11674810 5526CTGCTTTTCTRTGGTCACATC 0.066 0.068 0.274 0.100 0.154 11 2 1989401876434961 5527 TAAGCAAATTYGGAGATACAG 0.426 0.432 0.272 0.108 0.175 11 2198947953 1456556 5528 AAGCCAAGGAYTGGATCATTT 0.250 0.258 0.037 0.0940.121 11 2 198954384 16829186 5529 GATATGGTTCRGCTGTGGCGA 0.068 0.0680.051 0.239 0.124 11 2 198964749 4850838 5530 AGGAAATCTCRTAATTGAGAA0.106 0.106 0.041 0.114 0.359 11 2 198977567 12471228 5531TGAACTTAATYCTTTATCCAA 0.170 0.171 0.188 0.193 0.289 11 2 1989876021037663 5532 CCTCCAAATCSTTCCTGAGAG 0.357 0.369 0.281 0.125 0.346 11 2198995021 13398693 5533 CTTAATGTTAYGGTGATTTTT 0.909 0.920 0.430 0.4230.221 11 2 199003237 2529670 5534 TACTACTTTCSCTTAACAAGG 0.353 0.3570.375 0.446 0.367 11 2 199009513 13022622 5535 CTGACAAATCRAAGCAGAAAA0.493 0.521 0.992 0.552 0.359 11 2 199015891 2727767 5536TCATATGCTTRTTGTGACTTG 0.069 0.070 0.586 0.595 0.372 11 2 19901877916829952 5537 GCTTTTTCCARATAGAGGTGT 1.610 1.708 0.618 0.762 0.477 11 2199030492 10490082 5538 TCTGTATTATWCTTAATGGTG 0.145 0.148 0.527 0.5790.468 11 2 199042202 — 5539 CTAGGAATCTKGCAAAGTGGA 0.451 0.444 0.9000.562 0.436 11 2 199049683 12468958 5540 TGAAAGAGACRGAGTCTAAGA 0.2680.289 0.368 0.570 0.537 11 2 199056937 2467041 5541ATATGTATTCYGGCTAGACTG 0.885 0.922 0.455 0.578 0.381 11 2 1990635196744584 5542 TGTAAGTACAYTGGCTTTTAT 0.362 0.361 0.496 0.180 0.330 11 2199071714 2880591 5543 CTTTATAGGTRCATACTTATG 0.287 0.304 0.387 0.3410.360 11 2 199078721 2041080 5544 GGATATTGGCSTGAAGATGTA 0.558 0.5630.095 0.288 0.435 12 3 6629288 6774186 5545 TGAGATGGCASATTTTTAATA 1.0511.052 1.199 1.388 1.231 12 3 6641674 2323812 5546 CTAAAGCCCARTGATATATAA0.807 0.786 1.785 1.427 1.339 12 3 6654184 17045432 5547TCCAGCTGGCSAAACTCATGT 1.147 1.141 1.678 1.203 1.579 12 3 6661203 98431075548 GGCTGCTGCARGGAATCAGTT 1.403 1.442 1.261 1.774 1.602 12 3 666860917045515 5549 TTTAGCCTCAYTGGGATTCTG 0.195 0.199 1.060 1.777 2.133 12 36674962 17045532 5550 GTTGTCAGATMCCAAGGTTTT 0.287 0.275 1.167 1.3762.144 12 3 6681752 17045568 5551 CCATAGGGACMGGTGCACACT 0.471 0.497 0.5451.426 2.204 12 3 6687829 9822640 5552 TAAATCATTCKGGGGCTTCCA 1.403 1.3300.638 1.337 2.051 12 3 6694368 17045665 5553 GTTGGTCGCCMAAACACTAAA 0.2790.270 1.034 1.306 1.590 12 3 6700175 17045707 5554 GCAAAACATCYTGCAATATTA0.389 0.387 1.270 1.518 1.373 12 3 6715694 17045781 5555ATAAATGTTGYCAAATTTGGA 1.238 1.187 1.206 1.370 0.978 12 3 6723760 98176875556 AAATGCCTTAYGATTTAATCT 1.001 0.980 1.439 1.175 0.560 12 3 673233117460972 5557 TGCCTGAAAARTGGTAGGTAG 1.202 1.234 1.269 0.733 0.709 12 36745752 345233 5558 GGGATTGCTAYTTTAAGAAGT 0.640 0.608 0.697 0.651 0.62612 3 6753268 163966 5559 AGTAGGTAACRCCCATTTGTT 0.176 0.177 0.399 0.5230.513 12 3 6759934 347078 5560 GACTAATCAASATCAATTCAC 0.005 0.005 0.0530.388 0.362 12 3 6770637 345224 5561 GCAGCCATAASTCTCTAAAAG 0.295 0.2890.002 0.167 0.451 12 3 6780770 191525 5562 CCAGCTGTATWGGTTTTGCTT 0.0180.017 0.061 0.022 0.426 12 3 6789988 6781770 5563 TGTGGTTTGAYTACCTGAACG0.004 0.004 0.062 0.026 0.207 12 3 6798823 11714467 5564ACTTCTTTTGRCAGGTATGTG 0.779 0.765 0.023 0.070 0.239 12 3 680771212635360 5565 ACAGTTGCCAMCTCTCTACCC 0.024 0.024 0.168 0.112 0.098 12 36825351 6776458 5566 ACTCATTTCGKTTGTTTCCTT 0.036 0.036 0.375 0.076 0.06712 3 6834633 11929454 5567 ATTGTGCCTCRGCTCATAAAG 0.739 0.707 0.223 0.2990.058 12 3 6842256 17046136 5568 CACTTCCTAAYGGGAAACCAC 0.632 0.630 0.2240.331 0.181 12 3 6849819 437555 5569 ACACCAGCAGRGCGTCCAATG 0.297 0.2910.690 0.215 0.420 12 3 6858651 449379 5570 TACTCTTGCCRGTTTTATACT 0.0420.041 0.433 0.225 0.415 12 3 6868897 7651971 5571 CTAGAGACTCYACTAAATGCT1.217 1.169 0.278 0.546 0.495 12 3 6880612 340660 5572ACTAGGCTGASGGACTGTGTT 0.143 0.145 0.202 0.867 0.348 12 3 6887801 4600415573 TCTGCAGTAAWACTGAACAAA 0.289 0.285 0.678 0.595 0.546 12 3 68993311240966 5574 TGTTTGTGGGKGTACACACAG 0.041 0.042 0.848 0.616 0.936 12 36913008 9882865 5575 TGAAAGAATTRTGTGTCTTCT 1.216 1.177 0.768 0.663 0.76412 3 6920735 — 5576 TGTATATATCRTCTACGTGTC 1.532 1.535 0.792 0.533 0.63512 3 6928331 17046336 5577 CATCTTTGCTRTTTATTTTTC 0.027 0.026 0.836 0.9140.703 12 3 6939637 339013 5578 GGCCACTGACRGGAATAAAGT 0.302 0.315 0.6580.888 0.910 12 3 6946081 1516561 5579 TGCCCAAAATKAACTCAGAAT 0.116 0.1170.522 0.932 0.599 12 3 6952098 17693917 5580 ATATGGCAAARCTCAAAGAAT 0.8550.848 0.631 0.728 0.555 12 3 6956587 535178 5581 TTTTCATTTCRTGATTTACAA1.247 1.238 0.599 0.469 0.501 12 3 6969753 6443077 5582TTTTAGTGACYGAACGTGAAG 0.252 0.231 0.795 0.491 0.492 12 3 6978781 4831625583 TTTATGGTACRTCCTCATTCA 0.313 0.302 0.722 0.397 0.268 12 3 6986240802783 5584 ATAAAGAGCTMTGTCAGGTTC 0.565 0.542 0.229 0.364 0.101 12 36993932 781388 5585 ATGTTATCTTYACCAAAAACG 0.674 0.685 0.162 0.163 0.34212 3 7000186 655620 5586 GGTCAGAAAGRGATACATCAA 0.057 0.058 0.097 0.0250.300 12 3 7006247 28407476 5587 ATTGTCAATCYTGATCATACA 0.054 0.055 0.0230.030 0.286 12 3 7018599 2201924 5588 ATGTGTGTGCWTGGACTCTTA 0.048 0.0460.000 0.235 0.436 12 3 7024917 1240472 5589 GAAGGAGTTAYAGGGCTAATT 0.0440.043 0.005 0.150 0.230 12 3 7030300 17046535 5590 TATATGAGTTYGTTTGAATTA0.065 0.065 0.355 0.061 0.203 12 3 7036890 1154367 5591TAGGATAATAYGTTAAAAGGT 0.336 0.336 0.366 0.353 0.327 12 3 7043889 68005315592 CCTCTTTTGTRTTGTTAGCTG 1.668 1.609 0.357 0.402 0.277 12 3 7055901234790 5593 GAGTTTGCATSGTTCCTTTCA 0.052 0.054 0.990 0.382 0.241 12 37061757 1499147 5594 GTTAATGTGAKGAAGGGTGGT 0.066 0.066 0.975 0.673 0.27112 3 7068995 17046736 5595 TAGTTTGTTARTTGAACTCCT 1.566 1.514 0.263 0.7120.456 12 3 7077260 6443079 5596 TGAGTCAGGARCATCATTAAT 0.205 0.209 0.6640.779 0.961 12 3 7084915 1499156 5597 CAGTGGAACARCTGAGCAAAG 0.011 0.0110.737 0.349 1.055 12 3 7095417 — 5598 TTTAACTTGTRAGGTGTAGCC 1.025 1.0460.289 0.649 1.159 12 3 7102850 7625532 5599 ATTCTAGTTAYGTTCACTGGA 0.2420.243 0.298 1.367 1.793 12 3 7114845 6769115 5600 TTCTGTCTTCYGATCATTCAA0.000 0.000 0.799 0.967 3.559 12 3 7122158 17046783 5601TCATGAATTTYGGCATTTTTT 0.269 0.270 1.489 1.084 3.441 12 3 7128652 67780305602 TTGACTATCTRTGAAAACTGT 1.010 1.026 1.363 2.097 3.415 12 3 713631617234935 5603 TCATAATCTASGACTGGATAT 2.104 2.063 1.272 4.120 4.568 12 37142442 11915246 5604 TATTAGAGACYTGTTGGCAGT 0.405 0.400 2.306 3.9664.568 12 3 7148002 1878164 5605 ATGACACTTCRTCTACTTGAA 0.584 0.576 4.4883.643 4.789 12 3 7155162 6804466 5606 ACTTTATAGGYATACTGGTAG 2.722 2.4304.120 4.153 4.364 12 3 7162872 17234969 5607 ACAACCATGAYCCTGACCTTG 6.2784.966 3.886 4.789 4.187 12 3 7168821 7623514 5608 TCCTCAAGAAMGACTTGCATT0.651 0.690 4.312 4.966 4.225 12 3 7174495 6443093 5609AATGTAGAAGYGGCAGAAGAC 0.979 0.966 4.789 5.267 4.091 12 3 718751217235018 5610 AAACCTACATSATTCTGTATG 4.807 4.789 3.454 4.966 4.091 12 37193175 1400166 5611 TCATCCTTTCRTCTTTATTCA 0.785 0.786 3.093 5.267 3.88612 3 7198739 13082571 5612 GGCTAAAGAAYAGTACAAACC 0.735 0.742 2.601 1.8704.062 12 3 7204619 11708019 5613 CAGTTGTCTCRATGCCTAGTA 0.120 0.121 0.3541.694 3.924 12 3 7211588 11918486 5614 TTGCTCAGGGWTTCTATGATA 0.378 0.3630.122 1.687 3.665 12 3 7220908 2133440 5615 GACAGAAAGAMAATGGTTGTG 0.1110.107 0.020 0.521 1.766 12 3 7227190 10510354 5616 TGTTACAGAASGTATGTGTTT0.028 0.028 0.213 0.346 1.711 12 3 7233031 17638908 5617TGATAATTGASTAAAGGCAAG 0.083 0.078 0.504 0.252 1.455 12 3 7239504 64144605618 TTCAAGTGTTWGTTTGGAATA 1.117 1.030 0.503 0.430 0.371 12 3 72515491508713 5619 TATTTAATATYCTTGAAAGGT 0.929 0.856 0.629 0.420 0.285 12 37259458 870440 5620 CTCTGATCTAYTCCAGAGCTG 0.137 0.135 1.064 0.406 0.23112 3 7266260 2136153 5621 GTCTCTGATAMGGGAAACAAA 0.353 0.340 0.684 0.5110.267 12 3 7274331 9873905 5622 AGAAACCTAGYGGTAGCAAGT 0.870 0.845 0.2270.589 0.238 12 3 7286253 9829398 5623 TGAAGGGCCAYGTACTACCTC 0.339 0.3300.334 0.392 0.286 12 3 7295514 6443099 5624 CTTCACTGAAYGGATAGATAC 0.0680.069 0.331 0.207 0.316 12 3 7305179 7609608 5625 CTGGTCCTGCRTTTGGCTCTG0.388 0.389 0.179 0.214 0.369 12 3 7318584 17047063 5626GTTAACTTCARATACTTTGTC 0.331 0.325 0.176 0.230 0.243 12 3 7329377 98663935627 AGGATGAACARGTGACTTAAT 0.393 0.370 0.176 0.107 0.302 12 3 73365122030154 5628 TTGTTGCCTTWGATTATTATT 0.382 0.386 0.168 0.106 0.297 12 37344342 9311986 5629 GCAGTTGGATSACTTGATTTT 0.087 0.087 0.136 0.144 0.31812 3 7357930 17047171 5630 CTTTCTAGAGWTTACATGAGG 0.413 0.401 0.144 0.4080.217 12 3 7367719 17047199 5631 TTTGTCGAGGWTGCTAAAACC 0.165 0.165 0.1430.345 0.174 12 3 7379067 2221668 5632 AAATTCAAAAYGAGAGAAAAT 0.368 0.3630.685 0.370 0.228 12 3 7386549 1548146 5633 AAATAAGATTYTTTTGAATAG 0.3700.378 0.553 0.331 0.214 12 3 7394156 9883258 5634 ATCAATAGTGRGTGCTTACGG1.589 1.755 0.688 0.335 0.416 12 3 7400433 4686127 5635GCATTCAAGTMATTCAAATAT 0.105 0.110 0.630 0.347 0.379 12 3 7408728 14990795636 GCTGGTGCAGKTTCACGTTTT 0.458 0.459 0.516 0.388 0.531 12 3 7415256 —5637 TTTAAAGGCARTTGGAGAAAA 0.219 0.224 0.151 0.696 0.640 12 3 74206301066658 5638 GATATATGAGYGATGAAATTG 0.106 0.109 0.186 0.631 0.705 12 37426750 17700470 5639 TGACAACCACRTACAGCTCAA 0.474 0.478 0.519 0.4990.774 12 3 7433710 712775 5640 CCTCTGACTTYTATGAGCTTA 0.253 0.263 0.5050.620 0.799 12 3 7441454 17047321 5641 ATTCCAGTACRGGAGGCAGAA 1.440 1.4850.924 0.688 1.117 12 3 7448956 11717280 5642 TAGGAGTGAAYGCCAGTGTAA 0.1600.163 0.993 0.767 0.746 12 3 7461021 712786 5643 TCTATGGCTTRTAGAAGAGGA1.079 1.097 1.287 0.910 0.716 12 3 7467488 — 5644 TGGTATTTAARTGAGATACAT0.576 0.553 0.714 1.397 0.694 12 3 7473748 713291 5645TTTCGAATCCRTTGTGAAAAA 0.701 0.657 0.875 1.430 0.737 12 3 7481105 14500925646 AGCTCAGACARCAGTTGTGAA 0.490 0.482 1.210 0.780 0.751 12 3 7487728779708 5647 TTCCTGAACCRCAATTCCCTT 0.413 0.406 0.929 0.852 0.959 12 37499103 779705 5648 AAAACTGATAYGTAGGTAACC 1.720 1.853 0.584 0.591 1.09712 3 7508618 10222587 5649 CTGTTCTACCYGGCAAGGACC 0.137 0.132 0.493 0.4380.796 12 3 7517268 1375923 5650 TGATATTGGAYGATACTGATT 0.004 0.004 0.5020.596 0.893 12 3 7526367 924354 5651 CAGTAACAATKTTTCTTTAGT 0.298 0.2940.041 0.654 0.675 12 3 7532504 1145137 5652 TAAACTAGGGRACAATGACTT 0.3960.392 0.307 0.673 0.704 12 3 7540619 1144026 5653 TAGTTTTCTGSGTTTTGTTGT0.143 0.135 0.598 0.344 0.657 12 3 7552841 17721161 5654TGCTGGGACTYGGTTTCTGAA 1.200 1.222 0.667 0.379 0.553 12 3 7562251 7797365655 TTTTAACCAGRAAGTCTTTGT 0.699 0.664 0.728 0.574 0.466 12 3 7571446779719 5656 TCCCTTCTCTRTAATCAGCAG 0.523 0.511 0.790 0.631 0.324 12 37578708 1485179 5657 GAGTTTGAAAYTCCAACTGCT 0.501 0.513 0.553 0.536 0.46712 3 7579955 1145141 5658 AGGCTCAAGCRCAAAAGAAGA 0.228 0.234 0.472 0.5020.482 12 3 7592025 1531939 5659 TTTTCTTAATSTTTCTGCACC 0.731 0.680 0.3010.455 0.656 12 3 7604004 4686140 5660 AATTAGAGTAWGTAAAGCTCT 0.000 0.0000.142 0.488 0.682 12 3 7614708 3804883 5661 TCAGGAAGACRAAAGGACCAA 0.1210.120 0.440 0.358 0.672 12 3 7620217 11713266 5662 TTGTGCAAGCKAGTTAAATTT0.004 0.004 0.471 0.506 0.394 12 3 7629492 1485170 5663AGAATTCTTGRAAACAGCTTC 1.036 1.057 0.312 0.579 0.319 12 3 7635133 76406855664 AATGCCAAAAYGAACATTTAG 0.785 0.786 0.687 0.397 0.320 12 3 76437603804866 5665 GCTTTGGGTCWCAGCTTCCAC 0.102 0.099 0.910 0.304 0.285 12 37650322 9832222 5666 TACTCCTGCASCTTCTAATTC 1.019 1.011 0.478 0.368 0.32012 3 7656966 17724199 5667 AACTACATGCYACTGGGACAG 0.460 0.469 0.216 0.5010.298 12 3 7663564 4686146 5668 TTTCCTCAGGKAGCCATGTAA 0.109 0.109 0.2970.297 0.319 12 3 7680399 — 5669 GTTATGGAGASCATGGATTTT 0.116 0.109 0.1530.213 0.328 12 3 7685841 2324209 5670 GTCTAGCAAGSGGACACGTAT 0.327 0.3240.113 0.334 0.414 12 3 7691409 9845583 5671 TGCAACTAGAMTGGAGCTTTC 0.5130.513 0.191 0.233 0.251 12 3 7698179 10514663 5672 AGTGAATGTCRTGAAATACTT0.317 0.318 0.333 0.160 0.204 12 3 7706991 1354405 5673AATGAGTAGCRCACAATACTT 0.394 0.387 0.417 0.228 0.254 12 3 7713566 1627735674 ATTTATATATYGGGAGAGCTT 0.521 0.513 0.282 0.229 0.146 12 3 772078113099271 5675 ATATAAAACAWGATAACATTG 0.509 0.532 0.358 0.271 0.106 12 37733107 1857697 5676 AGTTTGTAAAYTGAACATTAT 0.128 0.124 0.255 0.246 0.11112 3 7744798 162723 5677 TTGACTGTGAYGGCACACACG 0.479 0.479 0.260 0.2210.115 12 3 7755175 17047887 5678 TAACCTCATCRTGATTTTAAC 0.152 0.156 0.1930.164 0.099 12 3 7761554 17047896 5679 CTTGTTCCAGRATGCTAGCTT 0.421 0.4280.205 0.126 0.110 12 3 7772492 161885 5680 GTCCTTCGAARTCAAAACAGA 0.3340.325 0.089 0.067 0.222 12 3 7780780 9817941 5681 ATGTAGCACAYAGGGTGATAT0.221 0.214 0.109 0.060 0.198 12 3 7786740 1473278 5682ATTTTTTTAGSTTCTAGACTC 0.088 0.089 0.042 0.057 0.410 12 3 7792722 98496335683 CTATTAGAGGRTAAGCAGACA 0.160 0.151 0.017 0.233 0.520 12 3 77981601550277 5684 AAGGCTGTTGYTAAGCTGTCC 0.166 0.166 0.064 0.185 0.611 12 37805201 — 5685 CATATTAGTCYAGTTTTACCA 0.124 0.121 0.421 0.586 0.574 12 37812783 11708939 5686 AGTTTGTGGCMTTTTACACTG 0.581 0.596 0.469 0.9290.617 12 3 7818918 1354400 5687 AGTTTGGCATMCTTTGGCATC 1.244 1.272 1.2931.207 0.587 12 3 7825284 6780542 5688 GACAATTATAYGCATTGTTTC 0.290 0.2951.827 1.215 0.625 12 3 7831694 17048003 5689 TGAATGGTACYAAGAAAGAAT 1.8171.908 1.973 1.265 0.660 12 3 7838157 162707 5690 GAAGGGTTTARAATCCTTGTT1.120 1.094 1.233 1.227 0.882 12 3 7850799 12488799 5691GCATAAACTCRGGAAACGACA 0.525 0.523 1.210 1.094 0.822 12 3 785979817048056 5692 TTGTGTTTTGKATCAGAGCTG 0.335 0.326 0.542 0.819 0.875 12 37871679 11718722 5693 AACCCTGGAGYCAGATTTGTC 0.351 0.343 0.343 0.9921.002 12 3 7877712 17740513 5694 ACAGGGCAAGRATGGTGTGAT 0.303 0.308 0.2860.523 0.864 12 3 7884368 9813540 5695 TTTTTGAGGTYGAAGCTTTAT 0.524 0.5010.469 0.336 0.621 12 3 7890962 401263 5696 GACATGAAAAYGGAATGAGAT 0.3620.351 0.401 0.354 0.572 12 3 7900483 378055 5697 GCGATTAAAARTAGATATTCT0.930 0.889 0.388 0.324 0.323 12 3 7909333 1001254 5698GAATGTTTTASCTAGCAGCCT 0.149 0.146 0.416 0.282 0.170 12 3 7924234 — 5699AGCGTTGATTKGCACTAGAGT 0.317 0.325 0.359 0.239 0.117 12 3 792978317048336 5700 CCCTTACATAWCATAATAGCA 0.602 0.618 0.145 0.169 0.122 12 37937060 7625080 5701 CACTCCAAATRCCCATTCCTG 0.167 0.165 0.118 0.128 0.10912 3 7942629 17048368 5702 AGACCTTTGARAAAGAAGTTG 0.157 0.156 0.111 0.0260.074 12 3 7950061 10510379 5703 TTATATAGTARTTGTCACGCA 0.058 0.057 0.0200.037 0.038 12 3 7957884 7652192 5704 ATTTGACAGTKTATTTACCAG 0.208 0.2030.006 0.038 0.027 12 3 7967737 7428715 5705 AGACTCTAATRTTATTTGGTT 0.1200.119 0.021 0.006 0.007 12 3 7980225 17693661 5706 TGTCAGTCCTRTATTTTCTGT0.035 0.034 0.043 0.004 0.005 12 3 7987346 4459890 5707GGTAATATGGYTTTTGAATAT 0.390 0.373 0.026 0.002 0.006 12 3 799424017048460 5708 AAACTTTTCARTAAACCATGT 0.000 0.000 0.034 0.007 0.001 12 38005030 17048480 5709 ACTCTCTGTTKCTAAACTAGG 0.105 0.104 0.038 0.0060.005 13 3 32775326 6785966 5710 GTAACTTAAAYGTTGTCTTCA 0.098 0.100 0.2620.195 0.110 13 3 32780201 6550159 5711 TTACTCAAGAYTAGTGCTCAG 1.185 1.1970.453 0.127 0.060 13 3 32801772 7619274 5712 CAGCCTGGGCRACACACAGCG 0.1190.120 0.406 0.128 0.024 13 3 32814515 6774262 5713 TGTCTATCATMTATTGTTATA0.578 0.577 0.373 0.115 0.019 13 3 32851743 7644738 5714ATGGGGGGAGYGGGGGCAGAA 0.243 0.320 0.067 0.076 0.019 13 3 328653186550166 5715 GGTCACAGGAMAACCAGAAAG 0.045 0.045 0.057 0.082 0.029 13 332879421 6550167 5716 TTGCGAGATAKAACTTAAGAT 0.077 0.078 0.009 0.0080.067 13 3 32890863 7612206 5717 CTACTACTACWACAACATATT 0.039 0.039 0.0020.011 0.057 13 3 32917788 1599831 5718 GTCTGGAGTASGTAATGCCAA 0.173 0.1780.003 0.005 0.114 13 3 32930887 7643959 5719 GGTATTTGAASAGCCACTCCT 0.0660.067 0.008 0.018 0.040 13 3 32942855 6786990 5720 TTTTCTTTGTKAAGCTCTTCC0.109 0.109 0.058 0.036 0.037 13 3 32954092 6550177 5721GGGAGAGGGAYGAGGGGAGGA 0.000 0.000 0.165 0.109 0.283 13 3 329709322228428 5722 TCCAAATTTAYTCTGCTGACA 0.388 0.382 0.300 0.189 0.452 13 332977803 6803961 5723 CCAAAGCAGCWGAGATCAGGT 0.660 0.677 0.587 0.1661.384 13 3 32995794 4640506 5724 TTCCTAATAARGAAAGATTTC 0.000 0.000 0.5871.039 1.309 13 3 33009602 4075736 5725 TTTCATAAAGRAGAGAAATAA 0.621 0.6410.456 1.414 1.316 13 3 33019646 4074708 5726 ATGGTTTCTCYATATTTTGGG 0.0000.000 1.434 2.229 2.381 13 3 33036155 6550190 5727 TCCCGCCCAGRCTCCACATCT0.103 0.103 1.566 2.054 3.159 13 3 33046631 6762132 5728CAGGAGGTATRAACAGTGCTG 2.293 2.228 2.616 2.048 3.604 13 3 330622046780220 5729 CTGCCTTAATMGGGGCTATAG 0.995 1.027 2.280 3.127 3.397 13 333074192 4438612 5730 GTGTCCTGCCRAGGTGGGAGG 3.074 2.951 2.595 3.8523.332 13 3 33085660 7610916 5731 TCACGGTGGGYGGATGGCTTG 0.034 0.040 3.4473.852 3.275 13 3 33121193 4578976 5732 TCCAGAGATAYGAGTTGGGAC 0.280 0.2823.551 4.062 3.217 13 3 33133916 4678686 5733 TGCAGCCTCARGTGCATCATA 3.1323.518 2.665 3.460 3.217 13 3 33151828 7652193 5734 ACTGTGGTTTRCAAAGTATAG2.210 2.073 2.568 2.756 3.332 13 3 33179004 4678490 5735TTCTTTGAACWGACGAGTAAG 0.000 0.000 2.807 1.901 3.447 13 3 331917384465894 5736 CTGGCAGAATRCCTGGAATAG 0.868 0.836 1.488 2.027 2.876 13 333204889 7636399 5737 TCCCACTCACYCCACAACACA 0.000 0.000 0.557 2.4432.775 13 3 33217501 4678764 5738 GGAACCAGAAYTAAAAAATTT 0.620 0.601 0.3801.261 2.269 13 3 33223374 4535176 5739 TCTTTAGCGCYGGCACTGAGG 0.169 0.1750.619 0.590 2.349 13 3 33243630 6798379 5740 ACAAAATTTGRTGGTAAAAGT 0.1620.147 0.449 0.918 2.446 13 3 33256297 6800399 5741 TACTTTGGACRGAATAGAAAA1.347 1.379 0.421 1.192 1.491 13 3 33267546 4678512 5742TTCGTGGTAAYAGAGGAGAAT 0.113 0.116 0.938 1.093 0.992 13 3 332735946798170 5743 CAGAGAGCCARCTAGTACTGC 0.531 0.529 1.523 1.076 0.944 13 333289743 4678832 5744 CAGTAGAGGGRCCAATTCTTG 1.423 1.447 1.086 1.0370.753 13 3 33318826 6807330 5745 ATGTGTGGTGKTGTGACACCT 1.551 1.558 1.3891.121 0.700 13 3 33348207 17029976 5746 CCACACCTCAMTAATCGGATG 0.3080.309 1.149 0.817 1.002 13 3 33350489 — 5747 AGTGTACTTTYAGCTGTGACC 0.5120.528 0.748 0.795 1.011 14 3 39582260 1620274 5748 TGGAAACAAGYTCTGAAGTAT0.565 0.553 0.266 0.407 1.009 14 3 39586705 816502 5749CAGCTCTAGGYAATCTGGCCT 0.278 0.281 0.156 0.616 0.979 14 3 396080667640123 5750 CCTCAAATGAYGTGAGTAGAG 0.419 0.411 0.199 0.951 1.085 14 339617791 9863522 5751 TGCCAGCACCRATGCTTTCAA 0.231 0.227 0.898 1.2871.122 14 3 39628600 4676652 5752 CTGTTTCTTTSTATTGCTATT 0.142 0.146 1.3731.177 0.766 14 3 39650458 4676655 5753 GTGATTGATAYGAAGGAGCAT 2.312 2.2521.823 1.160 0.835 14 3 39663717 4586749 5754 TTGTTTTGGARGTCCTAATTA 1.2131.162 1.677 1.169 0.687 14 3 39669773 1405796 5755 CAAGTAGGGAYGTGGGTTTTC1.397 1.468 2.082 1.040 0.899 14 3 39680713 10510704 5756CAGTGCATTAMGAGGACAAGA 0.050 0.050 0.900 1.184 0.792 14 3 3970050217759069 5757 TTTGGAATATYGATTACACTG 0.501 0.505 0.422 1.108 0.969 14 339742324 17039611 5758 AGTTCTGGACYGCCATATACT 0.200 0.198 0.155 0.7350.888 14 3 39751229 17076570 5759 GAATGACTCCYGTGAATGACC 0.180 0.1790.191 0.423 1.074 14 3 39764195 1405788 5760 AGTGTAACATYTACATCAATG 0.5680.551 0.373 0.350 1.054 14 3 39774785 1918028 5761 TTTCCTTAACRTTTTGCAGTC0.029 0.029 0.324 0.347 0.545 14 3 39784586 4676512 5762TGTGTTGGCCRGCCTATATTG 1.155 1.129 0.664 0.410 0.349 14 3 3979422411717218 5763 CACCTGTTAGYGCACCAGAAA 0.084 0.084 0.431 0.371 0.219 14 339800003 11717991 5764 ATAGAATTACRGCTGGGAACT 1.063 1.094 0.791 0.3730.399 14 3 39807162 7621402 5765 ATTGAATAATMTCTGTGAGCA 0.048 0.047 0.3220.287 0.373 14 3 39817037 17030291 5766 TACAGATTCAMCTTTCTAGTA 0.8270.804 0.362 0.427 0.788 14 3 39823345 1918029 5767 ACAATGAACAYCATTTAACAT0.018 0.018 0.105 0.416 0.836 14 3 39837056 9867310 5768ACATGAATACRGTTTTGTTGC 0.180 0.187 0.256 0.510 0.814 14 3 398484881918025 5769 CACCTGGCACYCTGTTGGTCA 0.215 0.212 0.346 0.838 0.842 14 339868177 28539759 5770 GTTTTCAGCTYCTAGCCCTGT 0.545 0.578 0.487 0.9230.614 14 3 39884611 7612386 5771 CCATATACTTYTGGGCCCTGC 1.131 1.136 1.5850.838 0.616 14 3 39895005 4676531 5772 CAACAGTATGYGCCTGGTACT 0.345 0.3501.636 0.892 0.453 14 3 39898848 — 5773 AGGCTTGTACRTTGGGCAACT 2.077 2.0791.583 0.916 0.561 14 3 39922940 7618607 5774 GTGTTCCTCCRTTTAGGAGAG 0.2390.246 0.978 0.886 0.417 14 3 39930070 1599903 5775 ATAAACTAGAMCCCCTGAAAT0.000 0.000 0.901 0.768 0.504 14 3 39936970 13060371 5776TCTTAGCTGCYGAGACATTTT 0.206 0.210 0.048 0.530 0.476 14 3 399493996803701 5777 TTGGCACACCSTCTGAATTAG 0.227 0.218 0.067 0.484 0.447 14 339962741 725296 5778 TACCAGAGACYCACCTACATA 0.051 0.052 0.101 0.074 0.36614 3 39967543 — 5779 ACTGAGAAACRGTGGCCATAT 0.383 0.362 0.100 0.052 0.16114 3 39978902 17183401 5780 AATTTGGAGAYGGATAAGGAC 0.477 0.480 0.1350.034 0.149 14 3 40028112 4309670 5781 TTAAGCTCACRCAAAGCACTA 0.223 0.2190.147 0.032 0.206 14 3 40037923 7637285 5782 AGGAGTTCACWTACAATGCAT 0.4100.411 0.090 0.021 0.294 14 3 40051924 7630287 5783 AAGGTGCACTKACATAAAAAG0.089 0.086 0.037 0.033 0.263 14 3 40069799 11920120 5784GTTAAGATACYGCCCTGTCTA 0.088 0.090 0.021 0.476 0.830 14 3 400769282887954 5785 CTTATTTCAARGGAAAAACAC 0.147 0.151 0.012 0.549 0.792 14 340082408 9816042 5786 AGCCTGGACTRTGTTTCTCAG 0.064 0.072 0.826 0.5561.465 14 3 40088394 17184770 5787 AGGGTCACGARGGAGCTGGCC 0.325 0.3231.182 1.309 1.413 14 3 40095453 6790823 5788 GACCCTCTTCSTTCTCCAGTT 2.4412.719 1.130 1.286 1.387 14 3 40105656 17075569 5789AGGACTCCAGYTTTACTGCTA 0.715 0.698 2.318 1.976 1.560 14 3 4011686111720413 5790 TGATGTTTTGSAACAGAACTA 0.253 0.259 2.020 2.073 1.626 14 340124462 1799414 5791 CAACTGCATCMGTGTCCCACA 2.459 2.389 1.850 2.1261.695 14 3 40132773 1616490 5792 CCTCCATGAGWTGTGTAGACC 0.053 0.054 1.7272.433 1.812 14 3 40142195 882822 5793 TGTAGGGATCRGAGAAATTGC 2.142 2.0741.829 1.790 2.253 14 3 40158347 7634072 5794 CAACAGCTTCRTGGTGTATTT 0.4460.441 1.211 1.570 2.686 14 3 40170306 12486603 5795CCTACAGAACRTCTTCACTAT 0.478 0.495 1.569 1.639 2.751 14 3 401806821799423 5796 TGTCAGCTCTRTTTTCAATCT 0.930 0.942 0.759 1.431 2.048 14 340194450 2255401 5797 TGAATGGCCAYAGTATAGAAT 0.681 0.683 0.775 1.8541.975 14 3 40201689 2371176 5798 GGAGCCTGTTMGTGCACAGTT 0.543 0.544 1.3831.365 2.336 14 3 40212567 2256927 5799 GTTATGCATCMTCTAGTTTAT 0.450 0.4571.585 1.637 1.572 14 3 40223397 2371129 5800 GCCTTGTAAAYTAGTCTTTCA 1.7661.771 1.513 1.838 1.958 14 3 40227350 1799422 5801 TCTGCCTGCTSTCCTCCAGCA1.537 1.567 1.738 1.778 1.921 14 3 40232784 1714408 5802AAAGGCCTGGMAAGCAAGGCA 0.516 0.492 1.746 1.465 2.264 14 3 402467011317217 5803 ATGATTAATAKCTGGAGAAAG 0.992 1.022 1.512 1.843 2.388 14 340249744 13092724 5804 TTTCCTGTTCRCCCCCATGGT 0.000 0.000 0.746 2.1412.357 14 3 40286329 4974028 5805 TCAATGAAGGRCAGCTGGTGA 0.939 0.952 1.3332.250 2.569 14 3 40301127 4974039 5806 TCATATTTGCKTTTAGCATTA 0.036 0.0361.959 2.238 2.542 14 3 40320412 9868700 5807 GGAGGCCTCTRAAGCCAAATC 1.6671.734 2.190 2.312 2.499 14 3 40327055 9826419 5808 GAATGTAATCRAGATGACGCA2.448 2.367 2.104 2.619 2.452 14 3 40337126 11718621 5809TATCACATCAYTCTACATATG 1.229 1.261 2.418 2.518 2.635 14 3 403425287652014 5810 TAACAGTGGAYAAAAGCCTAT 0.909 0.911 2.767 2.401 2.621 14 340349555 4974067 5811 AAGTCTAACAYGCAGCTCTGA 2.164 2.149 2.656 2.4392.569 14 3 40354011 9821036 5812 GGAGTTGTTCRGGGCAAGGAA 2.835 2.815 2.3052.669 2.578 14 3 40371258 17078511 5813 GTAATTAGAARTTAACAACCA 1.1431.141 2.331 2.705 2.654 14 3 40378879 9860162 5814 GGCTTGTAGTYAGCATACTTC1.939 1.966 2.606 2.568 2.816 14 3 40379774 10510708 5815TGAATCAAGTYGGTCTACAAT 1.516 1.521 2.394 2.596 2.735 14 3 403986269854493 5816 CTCTAGTCTAYAGTTAGCTAT 2.970 3.031 2.456 2.768 2.781 14 340404187 2305521 5817 ATGTTCTGAARAAAAGCCCCG 2.236 2.313 2.511 2.6892.772 14 3 40417584 — 5818 CTGGAGAGGTSCTGAGATGTT 0.463 0.453 3.082 2.5802.778 14 3 40424355 6783755 5819 TCTAAGAAGTYAGACAGACAT 0.374 0.364 2.6222.549 2.795 14 3 40431030 7645864 5820 GCAAAAGGGAYTGTGGTGTAC 1.021 1.0482.042 3.057 2.669 14 3 40433045 9332450 5821 TACCCTTATAYGGCAGAACTT 2.3262.340 1.963 2.718 2.623 14 3 40435771 13324109 5822AATGAACAAAYAGGTAATCTT 1.437 1.410 2.816 2.748 2.654 14 3 40447322 — 5823ATTCACATACRGACACCTGAT 0.412 0.402 2.552 2.761 2.613 14 3 404531556762997 5824 TCTTCCTAACYCAAGCACAGG 2.876 2.897 2.497 2.569 2.344 14 340462079 11707278 5825 ATTGGCTACAWTTAACAACAA 2.123 2.115 2.725 2.4562.020 14 3 40481552 6771527 5826 ACTGGTTCCCYTGGTAAGAGC 2.217 2.533 2.4802.068 1.824 14 3 40496322 4974029 5827 CACTGCAGTTSTGACAACTAA 0.931 0.9591.993 2.009 1.831 14 3 40506140 6762251 5828 AGATGCCTGARTATAGTAATC 0.4320.426 1.267 1.798 1.559 14 3 40515742 6599106 5829 AAATCCAGAGRCATATATTTT0.651 0.633 0.454 1.099 1.306 14 3 40534082 4973907 5830GATTTTTCAASAGCAACCTTC 0.026 0.025 0.234 0.710 1.104 14 3 405405986599108 5831 AATATGAAAARTAAGTTCTTT 0.345 0.344 0.165 0.291 1.096 14 340551065 3924444 5832 TTACCATGTGYCTGAATTACA 0.234 0.240 0.092 0.1250.703 14 3 40574409 4973908 5833 TGAGAGGACCRTCCAGAACAT 0.072 0.073 0.1770.103 0.403 14 3 40583767 6762428 5834 TCCCTTTCTCYGAGTGGTTTT 0.336 0.3460.082 0.050 0.169 14 3 40592543 6774841 5835 GTTGACTCTTYTGCTGGCTTC 0.3440.350 0.069 0.064 0.094 14 3 40609500 6599112 5836 CTCTTTTTGGWTTGGTAGGCA0.027 0.028 0.067 0.048 0.070 14 3 40624764 6599113 5837GAGGTTGGCASTGTCAGATCA 0.169 0.169 0.069 0.083 0.025 14 3 406345596772841 5838 AGGATAGCCCRTCTTGGTTTG 0.205 0.207 0.036 0.090 0.044 14 340647131 4577438 5839 AGGCTTTTTGYTTTGAGGGCA 0.294 0.304 0.177 0.0720.052 14 3 40665380 4383488 5840 TTGTCATCTGYGCAGTGTCCT 0.220 0.216 0.1950.026 0.180 14 3 40678197 7650783 5841 TGAAAAATGGYTGCTTTCTCA 0.625 0.6320.186 0.073 0.289 14 3 40686361 6599117 5842 AGGGCATACTRAGAGCCCCCA 0.2610.241 0.093 0.121 0.258 15 4 77038761 4859408 5843 CTCTTTTAGTRTATCTGAGGT0.264 0.267 0.409 0.175 0.435 15 4 77044119 324719 5844ATCCTTTAAGSAGATCTGTAG 0.166 0.166 0.211 0.157 0.409 15 4 770523183796479 5845 CTCATAAATTRCATAATACCA 0.095 0.094 0.022 0.223 0.439 15 477060174 17000863 5846 GAGCTTACTTRTCGTTACGTA 0.331 0.280 0.007 0.3810.401 15 4 77065861 17000870 5847 ACTTAGTCTTMCTCAACCGGC 0.019 0.0190.061 0.209 0.416 15 4 77076629 — 5848 TACATTGTGGRTATATTTGGT 0.029 0.0290.530 0.228 0.421 15 4 77083718 11728073 5849 CCTTGGATCTYTTAAGCCAGA0.664 0.690 0.474 0.407 0.396 15 4 77091522 17284996 5850ATATTAAATAYGTCTTTTTCC 1.509 1.633 0.623 0.445 0.237 15 4 77093390 9059545851 GTTACCTCTCYGACCCCACAC 0.155 0.153 0.992 0.458 0.191 15 4 77107327 —5852 GATTCTTTATYATTCTTTGTG 0.363 0.365 0.826 0.676 0.504 15 4 771150637696758 5853 ATTTCAAACTMCTCAGAGCAT 0.946 0.919 0.298 0.712 0.526 15 477122212 17000922 5854 GCAGGTGTCTYGCATTTGAGA 0.212 0.207 0.524 0.4740.679 15 4 77127596 — 5855 CTGTTTCTCCRTGTGCTCACA 0.323 0.337 0.398 0.5501.221 15 4 77145093 6811934 5856 TTAAAAAAAAYTAAACCACCT 0.745 0.719 0.1050.559 1.323 15 4 77157235 7669428 5857 ACTCAAACTCRTGTTCTTAAT 0.097 0.0950.694 0.771 1.320 15 4 77163081 1976518 5858 GTTCTGGCGAYGGGAGGCCAT 0.0020.002 0.610 1.217 0.851 15 4 77170444 17001165 5859GCACTACCCAYTGACGCCCAT 1.769 1.768 0.714 1.319 0.830 15 4 771812579784511 5860 CTAAGATGCTYGTGTGTGTGT 0.162 0.170 1.892 1.362 0.892 15 477196287 6819442 5861 GAAATTCCTCYGGAGGATAAA 0.918 0.930 2.026 1.0690.688 15 4 77204330 3733233 5862 CCAGGCAAAAYAAAATTATGT 2.059 1.991 1.1831.027 0.810 15 4 77222469 17001237 5863 TCTAGAATGCRTAAAGATCTC 0.3080.293 1.203 1.197 1.996 15 4 77228362 7154 5864 TGAACTAAATRTATTCCAAAG0.428 0.442 0.709 0.649 1.969 15 4 77234514 17001277 5865CAACATACTTYAGGAGTCATC 0.079 0.075 0.151 0.787 2.093 15 4 7723995017001284 5866 TTAAAGTCTTYAGGGCTATTA 0.066 0.068 0.149 1.864 2.311 15 477247035 17506007 5867 TCAAGAAAATRTGTATGCGTA 0.599 0.591 0.223 1.3121.784 15 4 77271152 6849878 5868 AATGTCTGGTRTATTGAAACA 0.285 0.275 2.1161.529 2.412 15 4 77287534 7670156 5869 CTGAGAGATGWAGGTGGATTA 0.665 0.6592.508 1.749 2.751 15 4 77291919 867562 5870 CTGTGTATTTRAGTTGTTTCC 4.0894.568 2.693 1.826 2.096 15 4 77313707 4859415 5871 TTAGTAATGCRTCTGATTGCA0.621 0.645 2.763 2.810 2.312 15 4 77322926 4859603 5872TTCTCTCTTCRACTTCTTTGT 0.000 0.000 2.788 3.353 2.953 15 4 773385234621456 5873 TTCTTCTTAASCTACTGATGT 1.047 1.097 1.443 3.643 4.267 15 477352642 4304003 5874 GACTCCTGACRAGGTACCTGA 0.194 0.195 2.738 3.8193.568 15 4 77359429 — 5875 GAAGAGTACARTTAGGCCCAG 1.702 1.743 2.514 2.4913.819 15 4 77365621 17001359 5876 GCGCACGTGTWCCATGTGTTT 2.290 2.2871.790 3.312 3.886 15 4 77378467 7654988 5877 CATTTAGACTSTGACCGACAA 0.5040.498 2.676 2.924 3.568 15 4 77384429 10028141 5878AAATATTATTRACACTTCAGA 0.381 0.376 3.139 2.816 3.102 15 4 7739164017001424 5879 AAACCCAACASGATGACTGGG 2.131 2.037 2.135 2.879 2.872 15 477402183 11733489 5880 TATATCTACGRTGATGCCTCT 3.192 3.245 2.591 2.6822.979 15 4 77416693 4481255 5881 AGAAAATCTTYACCTTCTGTT 0.499 0.503 2.4772.459 3.120 15 4 77430723 6824251 5882 CACATTCCAAMATAACAGAAC 2.199 2.1592.364 2.470 3.353 15 4 77439978 3733256 5883 GGAGGTGGAGSGTTTCCCCAC 0.3050.313 1.847 2.637 3.267 15 4 77448185 — 5884 TACCAGGAGGWAGTATGTACT 1.8961.817 2.092 3.052 3.007 15 4 77456563 17001573 5885TTATTTGGGCRTGTAGCTATG 1.087 1.010 2.133 2.961 3.127 15 4 774674906824953 5886 AGTCAGAGACSAAAGGACTTG 0.256 0.251 3.019 3.293 3.233 15 477474753 999361 5887 ACTTGGGCAGMAGCATGAAGC 2.357 2.479 3.130 3.114 3.25815 4 77481924 17001640 5888 TTCAACCAGCRTAAGCTTCCA 2.966 2.837 2.8832.988 2.953 15 4 77488248 17001659 5889 GCCCTGACTGYGGCCAGTTCT 1.1201.098 3.332 3.046 3.120 15 4 77501861 2034004 5890 TTATCACCGCYCTCTTCCTCT0.321 0.335 1.902 2.633 2.593 15 4 77534206 17001726 5891GTGTACAAACRAAGGATATAT 0.347 0.349 0.991 3.079 2.615 15 4 7754377517236158 5892 ACTGTCTTTGSTCTCTGTATT 0.315 0.330 0.634 2.150 2.082 15 477550724 1441911 5893 AGATTTAAGTKCCTAAGTTGG 1.392 1.383 1.065 1.0761.781 15 4 77559991 1530296 5894 TAGTGGCAAARCACCCTTCTT 0.364 0.373 0.9830.730 1.909 16 4 100854365 28666735 5895 CAATAGAGGGYTAAATTTAAA 0.3970.408 0.286 0.971 0.514 16 4 100861842 3816873 5896CTTCATCTAAYCCATGGAAAG 0.257 0.262 0.475 0.901 0.647 16 4 100869590982424 5897 CTGGAAATTGYAAAGTGACCT 0.239 0.234 0.840 0.672 0.636 16 4100878983 17029215 5898 TTCAAAAGTGMCAGCAGCATT 1.510 1.493 0.994 0.4210.822 16 4 100889431 2298747 5899 CTTGGGAAACWGTCATTACAA 0.827 0.8740.928 0.660 0.704 16 4 100908302 — 5900 AGCTCAGTCAWTCTTGTTTAG 0.6390.607 0.804 0.563 0.520 16 4 100914572 17029251 5901GCCAAGCTCARATCAGACTCC 0.115 0.116 0.517 0.815 0.360 16 4 10092117717600719 5902 TCTCCACGCCRGCCTGTGCCC 0.085 0.084 0.214 0.829 0.412 16 4100929924 17029267 5903 GTCTGATCACYGCTGTGGAGG 0.796 0.834 0.334 0.3740.355 16 4 100947301 17029309 5904 TGAATCATCARGTGCTATTGT 0.127 0.1220.413 0.199 0.883 16 4 100958504 13123629 5905 AGTCCACCCTMACCTAAAAAA0.967 0.993 0.408 0.142 0.909 16 4 100972443 17029332 5906TTTCCCCAGGYAGCTGAATCA 0.306 0.298 0.177 0.143 0.559 16 4 10098372710516451 5907 TTTACTAACCRTCATTACAAA 0.090 0.089 0.234 0.773 0.589 16 4100993990 4699759 5908 GTATTCAAATYGATCACCTAG 0.152 0.153 0.054 0.6370.460 16 4 101011970 6532828 5909 CCTAGCAAGCRCTGCACGAGA 0.293 0.2940.733 0.641 0.531 16 4 101019807 7675455 5910 TAGGCAAGACSTGGTATCCTA0.174 0.171 0.883 0.643 1.011 16 4 101026498 17537090 5911AATTCCATGARGACAGGTTTT 2.394 2.261 0.875 0.561 0.828 16 4 1010338181820495 5912 TAAAAGTTTGYTCACCTCCCC 0.465 0.455 1.185 0.677 1.320 16 4101041979 716556 5913 TACTAGCCAGRTTAAGTTCAG 0.087 0.085 1.156 1.3541.398 16 4 101062450 6831238 5914 CCTCACACTGYCTCCATGGTT 0.990 0.9890.315 1.336 1.306 16 4 101070943 11735070 5915 GAGGAAACAASTAGGAACAAT0.030 0.029 0.969 2.080 1.354 16 4 101082500 10031644 5916AGTAGGGAATKTATTTGCACA 0.464 0.475 0.971 1.554 2.812 16 4 1010955752282588 5917 GAAAACAAGTWGGTTGAGAAA 1.892 2.113 1.268 1.347 2.855 16 4101103675 10031708 5918 CAGAGCTTCTYGCATCATTTC 0.140 0.143 2.088 1.2693.551 16 4 101110330 2162386 5919 AACAGGTTATMTTTCAAGGCC 1.673 1.7611.728 2.536 3.585 16 4 101116777 — 5920 TTTGGTACGTKTTGTAGAACA 1.1361.131 0.820 2.801 3.434 16 4 101123160 4699387 5921TTTGACGACASCTACCGCTAT 0.188 0.185 2.531 3.924 3.761 16 4 1011297814699767 5922 GCACCTTATCYGGGCCCAAGT 0.232 0.225 1.924 3.447 3.924 16 4101130928 11935615 5923 CAGCTTGTTCRGGTCATGTTC 3.916 3.585 3.146 3.1024.187 16 4 101149661 6829912 5924 GCTGAGCCTAYGGTCTGCTCT 0.747 0.7453.342 3.342 3.924 16 4 101158451 11723286 5925 TTTATTTACARAGGCTATTTT3.996 3.761 2.961 3.503 3.503 16 4 101166087 11937985 5926TACTTTGATAYTATGAAAAAC 0.327 0.328 2.708 3.665 3.966 16 4 10118023611931053 5927 AATGGCCAAARTAGAAGACAT 0.859 0.872 3.434 3.761 3.761 16 4101183482 17029641 5928 TCTTACACATRAAGTATATGG 1.691 1.656 1.776 2.6333.422 16 4 101206644 17029667 5929 CTCATCTCCARTTTGACAGAG 1.131 1.1651.851 3.322 3.241 16 4 101209057 17613664 5930 AGTGTGTTCTYCAAAAAGATT0.816 0.815 1.655 2.238 3.187 16 4 101220468 17613746 5931AGCAAAAAATYAGGAAGAAAA 0.896 0.890 2.289 2.277 2.536 16 4 1012451506842840 5932 TTATAATTCARTTTGCAGCCA 0.416 0.430 1.971 2.415 2.471 16 4101258022 7689566 5933 AGAGCTATCARTTTATAGAGC 3.091 3.139 1.879 1.8531.876 16 4 101270627 6816635 5934 AGAAATGCCARAATCTCTCAA 0.843 0.8981.718 1.515 1.931 16 4 101278022 11932783 5935 TTATTTCATGKGATCCTTTCA0.106 0.110 1.799 1.428 2.229 16 4 101287921 6838495 5936TTATTTTAGAYATCTGTGGCA 1.359 1.438 0.495 1.479 1.910 16 4 101295878 —5937 ACATTTGCCCKTAACTTTAGA 0.067 0.065 0.530 1.682 1.666 16 4 101304621— 5938 GGTGATTAACYGCACTTGGCT 0.056 0.054 1.103 1.511 1.610 16 4101316549 — 5939 TGTTGTCAATKAAAGGGAAAA 0.951 0.986 1.086 1.441 1.464 164 101328817 17029724 5940 TTTAGTTGCARTTAAATGCTG 1.511 1.610 1.798 1.3521.465 16 4 101350212 6532842 5941 TGCTCATCTCYTCAGCTGACA 1.276 1.2621.782 1.114 1.014 16 4 101362457 7668076 5942 GGTGTACAAAKGATTTTTTCA2.358 3.071 1.519 1.121 0.961 16 4 101376717 4699391 5943ACTGGAGACAYGCTCAGCCCC 0.022 0.023 1.070 1.108 1.158 16 4 1013958537671736 5944 GATAATGAACRTTTTGTTATC 0.084 0.079 0.603 1.061 1.332 16 4101415183 4699777 5945 CTACATATGGYATACTATTTT 0.108 0.113 0.002 0.7341.382 16 4 101421476 17029744 5946 TTTTGAGCTCRTGATCATTCA 0.121 0.1250.056 0.655 1.560 17 4 126424833 1986513 5947 CTTAGTTTTAWCTCCACAGAA0.000 0.000 0.340 0.605 0.906 17 4 126433664 4349615 5948TTGCCCTCTCMAGGTTTTCTT 0.558 0.516 0.608 0.390 0.695 17 4 1264436214240276 5949 ACTGTGCAAARAAGTCGTGTC 0.518 0.518 0.766 0.741 0.707 17 4126456202 4532242 5950 AAAAGTAAATRTAGAAATCAT 1.070 1.091 0.678 0.5680.572 17 4 126478116 11721960 5951 TCATGTATAARTTTGTGTCAT 0.390 0.4100.957 0.906 0.473 17 4 126491841 4240277 5952 CTCATTTTCTKAAACAGTCAA0.384 0.293 0.750 0.809 0.628 17 4 126504147 17009345 5953TCTGACCAAARGTGTGCATGA 1.060 1.057 0.690 0.637 0.516 17 4 12651823317009376 5954 TCCTCTCACTSAGTTAATAGC 0.088 0.091 0.627 0.645 0.507 17 4126528678 17793037 5955 AATTCACTCARGAAGTATTTA 0.993 0.954 0.490 0.5370.487 17 4 126539380 17009427 5956 GTGTGGAAGARACCATACATT 0.255 0.2710.287 0.439 0.531 17 4 126554568 12512873 5957 ATAGAAATTTWGGACAGTATT0.044 0.044 0.547 0.363 0.486 17 4 126562521 10518462 5958TCTAAGTTTAYGTTTTGAGAC 0.538 0.530 0.208 0.191 0.382 17 4 1265738341355167 5959 AATAATTCTCYCCTTCAACAT 0.756 0.745 0.150 0.333 0.562 17 4126583321 11098804 5960 TATGGTGTTAYGTCAATTGTT 0.102 0.104 0.252 0.1860.512 17 4 126594846 7677005 5961 CGCTCAGACTRTCTGGATTCA 0.038 0.0400.300 0.260 0.313 17 4 126598940 — 5962 TGAAGAAAGASAGTTTGCTAT 0.3920.298 0.160 0.576 0.403 17 4 126613149 10034706 5963AATCCCATTTRCAAAGATCCT 0.667 0.674 0.340 0.468 0.237 17 4 12662272117801021 5964 TAGATGATTTSATAAAGACTT 0.355 0.338 0.823 0.281 0.299 17 4126628378 7676058 5965 AAGTGAAATTKTATATGAAGT 0.621 0.640 0.707 0.3970.405 17 4 126634576 17009520 5966 ATGATGATGAMAAAGTTGATT 1.219 1.1710.443 0.381 0.506 17 4 126643919 9307561 5967 AGTTAGGGAAYACCGGATGAG0.143 0.137 0.513 0.451 0.511 17 4 126659459 17009559 5968AAGCAGACTGYGAGAATTCTA 0.102 0.101 0.307 0.436 0.713 17 4 12666494613146586 5969 ACATTAAATGYCTTCTGTACC 0.573 0.455 0.146 0.660 0.761 17 4126671984 13146027 5970 TCCGTTCATTRAGAAACATTT 0.052 0.052 0.271 0.6891.223 17 4 126683303 17009611 5971 TATTGTATTCMTTTGATACGC 0.648 0.6430.690 0.640 1.079 17 4 126696793 4833325 5972 ACAGTTTAATYTTATTTTCCA0.619 0.630 0.798 0.702 1.112 17 4 126705065 6842220 5973TAATGAAAATYGCTTGAAAAC 1.061 1.033 1.293 1.399 2.245 17 4 1267119121395233 5974 AATAATATGGKAAGGTTGTGA 0.826 0.750 1.043 1.401 2.040 17 4126718936 9307564 5975 ATTGAATGAAYGATTTTAATA 1.073 1.058 1.758 1.5992.206 17 4 126726483 13108706 5976 CCTTATGGCARCTTTTCTGCA 0.399 0.3731.533 3.258 2.015 17 4 126732504 4834042 5977 CTAATGGTTGRGTAGAAAGCA2.270 2.290 1.345 2.890 2.283 17 4 126738202 17009708 5978GAAAGTCTGCRGATACCATGG 0.035 0.031 3.303 2.694 2.210 17 4 12674493217009734 5979 TGCTTCAGACRTTATAAAGGC 0.315 0.318 2.988 2.368 2.207 17 4126752525 12506486 5980 GGACATGGGAKCAAGAGGTGA 4.497 3.819 2.070 2.1702.046 17 4 126759250 17009779 5981 CACTAACTAAMCCTAATGGTT 0.364 0.3742.120 2.033 1.993 17 4 126765749 2068148 5982 CAGCATAACAYACTCCAATGG0.361 0.362 2.185 1.733 1.975 17 4 126771743 7696949 5983ACTCTTTGTAYGCCCAATTGA 0.130 0.126 0.429 1.748 2.255 17 4 12677733110518469 5984 TTTTAAACAGRTATTTGTCGA 1.327 1.340 0.620 1.802 2.266 17 4126784567 12643736 5985 TTGCCAGCCCYAGTTCTCTAT 0.121 0.119 0.605 0.5422.084 17 4 126791603 — 5986 AAAAACCCTARCAATGTGTTG 0.908 0.884 0.7891.035 2.058 17 4 126798362 — 5987 GTACAATGTGRAGGAAAGTAC 0.321 0.3210.459 1.170 2.060 17 4 126804720 17009923 5988 TGGGGAAAAGRGATTAAAAAT0.000 0.000 1.450 1.415 0.871 17 4 126817579 17009964 5989GCAAAGGAGARCTAGGAGGGT 0.598 0.603 1.193 0.973 1.034 17 4 12682069817818247 5990 AACAAAGAATRAAAGTGCCAG 1.692 1.676 1.529 1.118 1.240 17 4126829646 — 5991 ACAGCTCTACRTAATCGGGCA 0.693 0.717 1.168 0.940 1.447 174 126839872 13138789 5992 GCTACCCTAAYAGAGGCAGCT 0.792 0.799 1.140 1.2011.079 17 4 126873778 7441520 5993 TTAACCACAASTTTGCAAAAT 0.324 0.3080.528 1.326 1.091 17 4 126882430 1864363 5994 AGTCTTCTAAMCTTTCAGTCG0.348 0.338 0.645 1.414 0.865 17 4 126893281 1346715 5995TTTCAAGACTYGGTGGAAAGA 0.427 0.441 0.799 0.915 0.817 17 4 1269049716858650 5996 TACCTCATAAWCCACCCCCAA 0.937 0.984 1.060 0.706 0.786 17 4126911493 9307573 5997 CAGGACAGAARCCTTTTCGAG 1.149 1.188 0.911 0.5050.748 17 4 126921490 17010193 5998 GACAGGAGGAYGGGATTCTCG 0.895 0.9110.813 0.453 0.721 17 4 126933969 13142551 5999 GGTCTTGAGGYTGAGCAAATG0.134 0.134 0.480 0.489 0.680 17 4 126937821 17010216 6000TTCCTACTTAYGTATTTTCCA 0.111 0.110 0.128 0.481 0.652 17 4 1269490054466054 6001 ACCTGCTGTAYTGGGAAGAAA 0.117 0.104 0.052 0.618 0.679 17 4126960644 17222107 6002 GTTGTATCTTYGTTTTGAAGT 0.103 0.100 0.094 0.4400.721 17 4 126971660 1346716 6003 GAGGCCTGAARTGCTGTATGG 0.463 0.4690.528 0.372 0.749 17 4 126981792 7697425 6004 GGTAAACTTAMTATTCAGTCT0.398 0.396 0.741 0.433 0.623 17 4 127003431 7686488 6005ATATGATGAGRTGCATAAAAG 1.526 1.581 1.082 0.573 0.455 18 4 1430680471493023 6006 CCAGGCCCTTMTACCTTGGTC 0.202 0.189 0.387 0.562 0.141 18 4143079592 4956411 6007 CAATCTATGARCATTATCTAG 0.000 0.000 0.362 0.5320.124 18 4 143091542 4956305 6008 TAGAAATTCTRGCCAATCTTT 0.963 0.9590.912 0.498 0.109 18 4 143100742 977569 6009 AAAGAAGGAAYGACTAGAAAG 0.3460.362 0.982 0.406 0.149 18 4 143106130 17702304 6010ATTTTTCAGTSAATGATAATT 1.498 1.494 0.731 0.364 0.139 18 4 1431117272241911 6011 TTATCAAGGTMATTTTCCTTT 0.184 0.184 0.404 0.306 0.413 18 4143118755 17645963 6012 TGGCACCTGASGAAGACAGCG 0.049 0.046 0.260 0.2820.743 18 4 143127818 6843479 6013 CAAGTACATCMAATAAGAACT 0.118 0.1160.001 0.127 0.940 18 4 143136103 6817546 6014 TTGAGAATAARCTTCACTCTG0.000 0.000 0.004 0.424 0.967 18 4 143153576 11942550 6015CCTTTAAAATKTAAACACTTA 0.031 0.030 0.012 0.608 0.881 18 4 14316232917015129 6016 TTTGCTTAACYTTTCTCATTC 0.268 0.251 0.402 0.912 1.058 18 4143169736 2218314 6017 TTGTGCCATAYTTTCCTGCCT 0.305 0.237 1.407 0.9701.086 18 4 143180192 2840104 6018 TCTCCTAAACSATCTCTAGGG 1.693 1.7331.818 1.020 0.791 18 4 143193702 1909868 6019 GGAACTTACTRCAACAAGGCT2.172 2.290 1.752 1.568 0.780 18 4 143201428 6813602 6020GTGACCATTTYCTCACTTAAG 1.288 1.350 1.666 1.606 0.803 18 4 1432106221392779 6021 CAACAAGTCCRTAAGAGTCAA 0.316 0.322 1.954 1.572 1.266 18 4143264681 168074 6022 TCTCATACACRGTCTTCTAGC 0.111 0.110 1.132 1.4561.387 18 4 143274664 337237 6023 CCTGAGGCTGYTGTCCTTAAA 1.735 1.855 0.5171.041 1.817 18 4 143299633 1988510 6024 TCTATGGTTGYCACCACTGCT 0.3360.327 0.438 1.043 1.905 18 4 143305151 11100741 6025ATGCAGATGASTATGGTAATG 0.022 0.022 0.469 0.798 3.358 18 4 1433118904245935 6026 GGGTCTCCAARGAGGCTTTTA 0.117 0.114 0.585 1.219 3.348 18 4143318966 1912714 6027 TGTCCAGGAGSGTGAAGCCTT 0.188 0.185 0.626 1.2912.726 18 4 143325686 3775605 6028 GCTCACCTTAYAAGGCTTATA 2.082 1.9831.516 2.213 2.252 18 4 143328144 2645800 6029 GTCTGGTTTTRTGTTTGTATA0.457 0.442 1.574 2.892 2.467 18 4 143343738 336376 6030TTTGCAGTTTKAAAAAATTTA 1.437 1.523 3.869 3.156 2.800 18 4 14335194912501475 6031 ATAATGATGTYATTCACACTC 0.065 0.067 3.221 3.317 2.384 18 4143358319 10519618 6032 GTTGTATGTGYGGTAGGAAAT 3.791 3.332 3.337 3.8352.432 18 4 143364802 336325 6033 TGACACCTGGYAACCCAAAGT 1.119 1.209 2.2452.622 2.469 18 4 143374465 336300 6034 AAGTCAACATRGATTGCAAAG 0.407 0.4052.842 3.082 2.529 18 4 143380368 336330 6035 GTGCAGTTTGYTAAAGAAGTC 0.0600.061 0.551 2.219 3.076 18 4 143390434 336336 6036 TGTTATCTACRTTTTCTGTAA0.849 0.853 0.535 2.127 2.159 18 4 143403715 2667083 6037GCCACCCATCRGACCTCTCCA 0.162 0.161 0.561 0.615 2.224 18 4 1434139017697702 6038 TCAACAGCAAYGGTAGATCCA 1.129 1.110 0.549 0.570 1.941 18 4143420416 336396 6039 AAAACCTTGGYTCTCAAACAA 0.476 0.469 0.403 0.4962.032 18 4 143429323 336405 6040 AACTGGCTATRAAATTTTCCT 0.070 0.070 0.8180.670 0.769 18 4 143439909 17015614 6041 TTATCACCTCYTAAATGCTCT 0.4830.486 0.386 0.726 0.492 18 4 143448657 173045 6042 TATTAAACAASGTCTATCAAT1.024 1.089 0.404 0.823 0.527 18 4 143454027 1219271 6043GACACCACTARGAAAAACTGA 0.158 0.161 0.898 0.544 0.526 18 4 1434607483822146 6044 CTACATTTTAYTTCTCTCTGG 0.538 0.520 0.873 0.421 0.566 18 4143468215 3113519 6045 CAGAAACGGCRTACAAGAAAA 1.052 1.008 0.484 0.5710.888 18 4 143475449 4574489 6046 TATGAGTGGCYGGTCGGTTAC 0.517 0.4860.444 0.423 0.707 18 4 143489842 10452237 6047 AAAAAAGGTAYGCATTCTGAA0.218 0.217 0.446 0.438 0.728 18 4 143502133 7672890 6048TATGTGCCAASTAGTAGATAT 0.115 0.113 0.126 0.862 0.826 18 4 14352007917015781 6049 TTTTGGACCAYATCATTGTAT 0.539 0.532 0.296 0.776 0.811 18 4143530628 17654944 6050 ATTCATTAACYTGATTATCAT 0.018 0.016 0.851 0.6500.665 18 4 143552356 3102439 6051 TATCTATTCCSTTTCTTAAAG 1.053 1.0570.927 0.648 0.945 18 4 143559553 3113513 6052 ATTATTAAAAYGTTATCCTCT1.568 1.508 0.994 0.727 0.895 18 4 143565954 2627813 6053CTATGACGTGYTTCTGAAGTT 0.362 0.368 1.198 0.840 0.876 18 4 1435722542636632 6054 CTATTACTTCYGTCTTATTCT 0.639 0.647 0.922 1.137 0.775 18 4143579022 17015855 6055 TTTATCTTTTWATCACTCTGA 0.437 0.439 0.431 1.2510.852 18 4 143585347 1907108 6056 ATGAAAGGCTRCATCCACACT 0.400 0.4220.851 1.208 0.948 18 4 143592198 2636670 6057 TGATGAGAGTRTGCATTTAGG0.442 0.445 0.655 0.658 0.908 18 4 143598256 17015920 6058AACAGAAATGYTTTGCCCTGC 1.273 1.326 0.882 0.706 1.097 18 4 1436011612635429 6059 AGTATCATAAYGGTAGTGTTA 0.264 0.249 0.737 0.640 0.930 18 4143619115 2635421 6060 TGTGTAACAAYGTTCCCCTTT 1.006 1.010 0.769 0.5950.623 18 4 143631056 1391093 6061 ATTGGCAGTAYTAAGACAACA 0.037 0.0370.424 0.691 0.638 18 4 143639478 1497397 6062 ATAATAGTCAMGATGATGGTT0.543 0.529 0.430 0.723 0.831 18 4 143647804 11938760 6063AAGGGTTCAARAAATGAGGAA 0.407 0.413 0.334 0.442 1.556 18 4 14365533717016027 6064 CACTTCAAAAMGTGAGTCCCT 0.304 0.307 0.567 0.487 1.812 18 4143680514 13135641 6065 AAAGCCAGTAWATGGAACGGA 0.771 0.789 0.416 0.6422.133 18 4 143688658 1489578 6066 TCTGCTCCAGYAGTTCTCACT 0.616 0.6020.431 1.769 2.019 18 4 143695802 17016097 6067 TCGTGGGAAAKAGTAGTTCTA0.196 0.194 0.965 2.228 2.573 18 4 143702078 3756125 6068GTCTGTAAAGYGATGAGACAT 0.419 0.437 2.491 2.687 2.735 18 4 14371284710428305 6069 CAATCATCTAYAGAGACCGAG 1.631 1.529 2.983 2.662 2.691 18 4143718806 931637 6070 CTGTCAGAACRTAAATTTGAT 3.912 3.654 3.447 3.1272.738 18 4 143739539 17016163 6071 GATGCCCTTTSCAACACATAT 1.868 1.7563.397 3.364 2.730 18 4 143752462 3913164 6072 ACTTCTGCCAYAACTTGGCTG2.760 2.658 3.460 3.187 2.720 18 4 143764438 3844178 6073TTAAGAAATARGGAACTGAAC 0.921 0.914 2.845 3.114 2.787 18 4 1437735291425537 6074 TAGTCCCACAWAAATCTCATT 3.010 2.879 2.696 3.073 2.909 18 4143779157 12646243 6075 TAGTAATTGTRGATTCTAGCC 2.912 2.767 2.488 2.5362.858 18 4 143802349 13107432 6076 TCTTCCCACARTGTCCCTCCT 0.683 0.6322.425 2.471 2.789 18 4 143810140 10857395 6077 ATAGGTTTTAMTGGAAAATTA0.404 0.408 1.383 2.061 2.740 18 4 143818067 2059513 6078ATTTGACTGASGAATTTTGCT 0.597 0.593 0.263 1.779 2.297 18 4 14383030117016354 6079 ATTTCAGTGAYGTAAACCCCT 0.063 0.063 0.151 0.839 1.899 18 4143839071 16998560 6080 GACAATCAGGSTCCTCTGTGG 0.097 0.096 0.082 0.1461.223 18 4 143845700 1473222 6081 TAAGGACTACRTTCAGAGCCT 0.309 0.2770.069 0.146 0.977 18 4 143851164 1425523 6082 CTGGTGTAGTRGTCTCTTAAG0.119 0.119 0.068 0.115 0.416 18 4 143865944 7688435 6083TATCCTGTAAYGGCCAGTGGG 0.549 0.528 0.225 0.052 0.089 18 4 1438739791364925 6084 TCTCTTAGTGSTATTTCCTCT 0.077 0.072 0.193 0.050 0.070 18 4143886618 13120964 6085 TTCCACTATTRCCCTTAATTC 0.689 0.703 0.168 0.0690.121 18 4 143896248 10857396 6086 CAGGACAGCAYCAGAGGCAAA 0.163 0.1610.056 0.079 0.096 18 4 143900941 1443191 6087 CTCCCAAAAARCTCAATGTGG0.084 0.079 0.071 0.111 0.136 18 4 143922224 1373039 6088GAGCAATTCARTGGAGGTGAA 0.023 0.024 0.037 0.168 0.139 18 4 14393334513125853 6089 GCTTGATTACRTTTTTCTTGT 0.154 0.148 0.074 0.220 0.127 18 4143946527 17016550 6090 AGAAGGAGGCYTCACAGTAGA 0.515 0.508 0.290 0.1720.141 18 4 143957383 1838087 6091 AACGTAAGAGSATATAAAAAG 0.345 0.3470.380 0.136 0.134 19 6 27042873 — 6092 AAACTGTGGASAAATTTCTTT 0.000 0.0000.001 0.002 0.462 19 6 27078381 9357029 6093 GAGCCGGGCCRTGAGCGCCTG 0.1230.121 0.007 0.022 0.463 19 6 27091877 7752299 6094 GCATTAAACCYTTCATGACTG0.059 0.057 0.041 0.177 1.802 19 6 27104110 6913271 6095CATACTGAGTSTTTTAATCCG 0.220 0.211 0.091 0.351 1.152 19 6 271174917775041 6096 TTGACTATATWCATGCATTCT 0.324 0.335 0.531 0.492 1.522 19 627155895 994690 6097 CTGAAATAACYCCTGAGGCAA 0.591 0.605 0.879 2.191 1.26719 6 27162476 2142685 6098 TTTATGTTCTYTTGAAGATGA 1.418 1.317 1.033 2.0481.337 19 6 27172468 — 6099 GTATTCACTCRAGATGTTAGT 0.757 0.754 3.327 2.3501.489 19 6 27183462 9348752 6100 AGAATGTTCAYAGATATTTCT 0.462 0.468 3.0492.355 1.382 19 6 27201800 6456768 6101 TGGAGGATGGSATAGTAAAGA 4.046 4.0113.024 2.137 1.451 19 6 27211559 2022272 6102 ACAATTAAATRTACAGTGGTT 0.0000.000 2.029 2.150 1.577 19 6 27222031 9393790 6103 TACTCAGTGTKATAAGATCAT0.785 0.758 1.901 1.807 1.659 19 6 27239937 4582379 6104TGATTAAAAGRAATTCAAGAA 0.090 0.095 0.291 1.480 1.703 19 6 27248129 — 6105GCTTGCAGAGRTACTAAACCA 0.284 0.277 0.370 1.513 1.630 19 6 272727366901448 6106 GAAAACTAACMGTTTGAGGGT 0.751 0.733 0.235 0.187 1.167 19 627281378 4711143 6107 CTTGTCAGTTYTTAAGGACAG 0.327 0.323 0.368 0.2970.936 19 6 27309252 3800309 6108 ATGATTTTTTMTCACCATGGA 0.367 0.366 0.3140.247 0.901 19 6 27318564 3800311 6109 CAACAGCTTCRTAGCAAAAGG 0.512 0.4840.251 0.223 0.138 19 6 27324467 9468021 6110 GTGAGCAAGTRAATAAACAAG 0.0000.000 0.352 0.201 0.145 19 6 27331221 3734577 6111 CATGTGTCTGYAAACATCCTT0.527 0.519 0.208 0.133 0.074 19 6 27337540 11758636 6112TCCGATGGCTRTTCCCTCACA 0.500 0.507 0.102 0.149 0.083 19 6 273438456915678 6113 AGACCAAGAGKTCCGCTGCCA 0.028 0.029 0.177 0.106 0.159 19 627364197 16897282 6114 GCCGCTCCAGMGAGTTTAATT 0.145 0.140 0.128 0.0460.144 19 6 27371321 16897348 6115 TACTGTTGCTYATACAAATCA 0.356 0.3450.044 0.066 0.112 19 6 27379322 7771953 6116 ACAGAACGCGRATCCGTTGGA 0.3990.390 0.049 0.113 0.090 19 6 27385644 2281023 6117 TGGGAAAGGTRGCCTTGAGCT0.101 0.099 0.073 0.133 0.078 19 6 27400907 16897710 6118TTTGTGTATGKACTTCTTCTC 0.073 0.073 0.226 0.133 0.136 19 6 274066089379978 6119 GAGGAAAACCRAAGTCACTAG 0.281 0.272 0.300 0.138 0.128 19 627412565 6903160 6120 CAATTTTTACWGTTTTTCCAA 0.941 0.862 0.298 0.1530.087 19 6 27428723 2393926 6121 CTGATCACCCSAACAAAAAGG 0.631 0.615 0.3270.206 0.165 19 6 27438664 12213055 6122 TATTCTGTCTYTTACAAGAAA 0.0450.046 0.370 0.303 0.301 19 6 27452810 6456774 6123 AGGGAATTGTSAACAACTGAG0.216 0.177 0.274 0.323 0.439 20 6 152286110 1709183 6124TTCCCTTGTAYCGTTGTGGCT 0.172 0.173 0.597 0.636 1.021 20 6 1523008361913474 6125 AAACCTTCTTRGAACCCTAGG 1.921 1.863 0.446 0.745 1.033 20 6152316850 9322341 6126 ATTGCTAGACYTAATCATCCA 0.057 0.057 0.737 0.4791.131 20 6 152327453 12204714 6127 TCAGGGCTCAYAATTTCTTTG 0.023 0.0221.100 0.952 0.787 20 6 152355742 6915819 6128 CTTAAAATATRTAGTAAGTGC0.852 0.855 0.187 1.087 0.700 20 6 152369037 3020391 6129TAAACTCATGRCTCGGGGCTT 0.757 0.789 0.846 1.287 0.715 20 6 152378739985694 6130 ATATAATACAYCCCTGAAGTT 0.006 0.006 1.133 0.471 0.657 20 6152392741 9340939 6131 GTCTCATAATMATAAGAGAGT 1.534 0.856 0.939 0.5440.662 20 6 152402727 6916218 6132 CAAAAGTATTWAAAAAAATCA 0.510 0.5140.558 0.817 0.675 20 6 152408861 17081994 6133 TTCTATTTTCYAGTTAGACTT0.000 0.000 0.685 0.569 0.315 20 6 152424244 9340969 6134AACCACAATAYTATCAGTAAT 0.059 0.065 0.382 0.439 0.322 20 6 1524317297755185 6135 TGTGTTTTTGRTTCACATGAA 0.234 0.244 0.235 0.507 0.322 20 6152435877 3020411 6136 CAGTTTTTTGRGAAGTAGAGG 0.753 0.812 0.217 0.1800.259 20 6 152450293 2982712 6137 TGTATTGCAGYGCCAATTGCT 0.131 0.1330.271 0.090 0.222 20 6 152459232 3020364 6138 CTGGAATAACRCCTGAGATCT0.242 0.254 0.211 0.044 0.248 20 6 152472491 9397079 6139ATTTAAACATRAAAGCATGGA 0.264 0.268 0.033 0.080 0.071 20 6 1524773503778080 6140 AAAACCCGGAMTCATCCATAG 0.107 0.110 0.016 0.110 0.054 20 6152500240 750686 6141 TTCAACTCACRTAATGAGAAG 0.037 0.037 0.028 0.0400.079 20 6 152514449 2747648 6142 TTGTTGGATAYTGAATGACAG 0.004 0.0040.071 0.047 0.166 20 6 152525016 910416 6143 CTGCTTTACAYGTGGTCTCAG 0.4430.468 0.099 0.048 0.159 20 6 152533353 2813549 6144TCTTCAGTGAYGGGAGATGCC 0.531 0.530 0.144 0.091 0.118 20 6 1525420149397486 6145 GATTAAACACRAGATATCTCA 0.201 0.198 0.237 0.236 0.169 20 6152550188 2813566 6146 AGGATGGCTARGTAAAAGACA 0.154 0.150 0.293 0.2890.182 20 6 152556953 2256135 6147 GTATCTGAAARCGGCTATCAT 0.332 0.3300.425 0.394 0.269 20 6 152563317 2813493 6148 ATATCCCGATSATTTTAATTT0.607 0.620 0.443 0.431 0.485 20 6 152570021 6937954 6149ACCAAGGGCCWGTATATATGC 0.957 0.962 0.557 0.388 0.482 20 6 1525768774870079 6150 GAGATATTCTRTCTTTGCTAC 0.310 0.296 0.705 0.558 1.080 20 6152584631 2763031 6151 CAGCAAATACKGGAGTGACTG 0.446 0.455 0.577 0.9281.282 20 6 152590550 2247922 6152 TGTGATTTAASTGACGATGAA 0.621 0.6220.531 0.840 1.275 20 6 152601623 2635430 6153 GCCTTTCCACSAATCTTTCCC0.350 0.373 0.918 1.504 1.466 20 6 152615664 2253407 6154CAATATGAAGKTATGCCCACA 0.815 0.834 0.791 1.660 1.694 20 6 1526213743798756 6155 GGCTGTCAATRATCCCCCGCC 1.280 1.321 1.748 1.714 1.854 20 6152627649 10872683 6156 TTTGTCAGTGYTATGGGAAAG 0.029 0.029 2.190 2.0141.690 20 6 152641700 12528883 6157 AGGTGTCTCCSTGTTAAATAG 2.670 2.4102.070 1.925 1.340 20 6 152647226 2296254 6158 AAACAAGTAGYGATGTTCAAA1.339 1.328 1.940 2.146 1.343 20 6 152652592 4869757 6159GCAGATAGCCMAATGTACACA 0.350 0.345 2.256 1.912 1.256 20 6 15266238817215781 6160 TTTCTCTTCTRGGAAAAGGCA 0.548 0.575 0.908 1.201 1.238 20 6152667818 7763242 6161 ATATTCTTTCKCAATGTGTTT 0.485 0.488 0.419 1.2201.505 20 6 152680602 9383983 6162 CCTGTATATCYTCTCAGTTGA 0.684 0.6780.290 0.406 1.727 20 6 152691531 — 6163 CAGCATATTCWGCAGCAACAG 0.2330.228 0.234 0.219 1.392 20 6 152706238 630548 6164 TGCATTTTAAMTTGACACATT0.039 0.037 0.106 0.387 1.464 20 6 152716034 674724 6165TTTAATTTCAYTTGCTTTTGA 0.388 0.381 0.092 0.734 0.941 20 6 15272438512206761 6166 GCTCCTGCTCRGTCAAGCCCA 0.027 0.027 0.350 0.645 0.892 20 6152733065 1472023 6167 TAGGAAAATTRATCCAGAGAA 0.606 0.556 1.055 0.5511.567 20 6 152739795 2306916 6168 AGCTGTAACAWTTCTTGGGCA 1.111 1.1161.057 0.794 1.449 20 6 152745878 725235 6169 ATGTGCTTTCYTTTGTGAAGA 1.7441.689 1.197 1.248 2.080 20 6 152752424 4870095 6170TCAATCTCACRTGAAACATTC 0.213 0.210 1.397 1.981 2.116 20 6 1527589987738089 6171 AAAACTCTTTKTAGTGTCATT 0.375 0.375 1.447 1.893 2.340 20 6152767168 6905339 6172 AACAATGTGAYACAAACTGTA 1.031 0.950 1.613 2.4953.130 20 6 152774905 6557214 6173 CAGGCTGACCRATGATCATGC 1.147 1.2011.751 2.636 3.034 20 6 152781309 9371592 6174 AAAAATGGGTRTAAACATAGA2.447 2.328 2.676 2.278 3.237 20 6 152789820 214955 6175GAAAAGGTGAYGCAAAAATTT 0.630 0.617 2.564 3.460 3.559 20 6 152810619549981 6176 TCCTCAGGCCYTTTCTTTTCC 2.950 2.714 2.634 3.623 3.988 20 6152819217 17082645 6177 CATAGCCCAAYTACTACTTGA 0.581 0.593 3.245 3.4743.676 20 6 152828926 761408 6178 TGTTTAAAAAYAGATAATAAG 0.670 0.668 3.3923.403 3.988 20 6 152838247 6909684 6179 TATATTAAAAYGAAATGGACT 3.2923.011 1.824 3.835 4.153 20 6 152848308 17082676 6180ATATAAAGTCYTCAAAATTTC 0.000 0.000 1.696 3.905 3.748 20 6 152854769214996 6181 GTAAAGTCTTRGAAATGTAAA 0.783 0.754 2.876 3.403 3.905 20 6152861412 17082700 6182 GTATCCCATAYAGCAAGAAGT 0.520 0.532 2.301 3.8353.676 20 6 152868292 214970 6183 GTGACACAGTRTCTATACTAT 3.367 3.258 3.1303.595 4.225 20 6 152875047 579464 6184 CTTAACTTCCRGATTCCACAG 1.791 1.7333.698 3.633 3.835 20 6 152887970 7755437 6185 ATTCATAATTMCTTATATTGA0.000 0.000 3.481 3.869 3.869 20 6 152898003 9397512 6186TGTTGCCATAYCACTGTGTAG 4.278 3.654 3.034 4.312 3.869 20 6 1529131249479327 6187 AATTTATTTTYTGGATGATTA 0.000 0.000 3.428 3.869 3.496 20 6152925152 11155857 6188 CCAACAAAATRGACTTACATG 1.043 1.023 3.723 3.2063.676 20 6 152934088 17699371 6189 CTGTTAGACAYGACACAGTCT 0.217 0.2060.689 2.672 4.568 20 6 152940828 11155858 6190 AGCCATATACRGTCATTAAAA1.573 1.505 0.811 2.550 4.568 20 6 152941489 2141151 6191GCGACCTCTCMTAAACTTTGT 0.061 0.062 0.531 0.566 3.676 20 6 15295624211968876 6192 AATATAAATTSGCATAATATG 0.296 0.312 0.789 1.289 3.698 20 6152964386 9397523 6193 CAATGATCGGMTAGTAAAGTA 0.429 0.431 0.231 2.3863.262 20 6 152979559 2623964 6194 CAAGCTGAAGYGAGCAACTAG 0.789 0.7441.137 2.424 1.633 20 6 152989528 7749912 6195 TAAGGAATTASTAGGCATTTA0.245 0.251 2.494 1.852 1.802 20 6 152997160 2178009 6196ACTCAACATTRCCTAAAAACT 1.999 1.908 2.240 1.735 1.873 20 6 15300305317701297 6197 AACCTTAGCTRGATCCATTTT 2.910 2.696 2.287 1.709 2.276 20 6153011820 10457880 6198 TCCCATAATGWATCAGCTTCA 0.042 0.042 2.208 1.6762.172 20 6 153031553 1744387 6199 AATCAGTGTARTTCTTGGCAA 0.788 0.8261.319 1.989 2.278 20 6 153037891 1407488 6200 TTTAAATTGAKTAAAGTTCCA0.271 0.268 0.216 3.004 2.837 20 6 153044292 9383634 6201TGACACAGAGRCTATTTTTGA 0.178 0.178 0.626 2.203 2.792 20 6 153058027 —6202 CCTTACGTGTRTGTAGTGCAT 0.483 0.465 1.025 1.082 2.516 20 6 1530700692984656 6203 ATGGAGGAGAYACTAATGAAG 1.076 1.052 1.330 1.620 2.803 20 6153078353 2758768 6204 TTAATTTACCYTGTCTGATAC 1.525 1.458 1.659 1.6592.258 20 6 153083723 873889 6205 CTGAGAACTCYGGGCTGTTTC 0.938 0.919 1.9301.611 1.436 20 6 153090503 2758774 6206 TTCAGCCCTGMTCTCCATTTT 0.7390.748 2.076 1.660 1.534 20 6 153097114 2758775 6207AAAACTCAACWTATGGAAGGC 1.391 1.785 1.537 1.806 1.422 20 6 1531093541936726 6208 CAATCAGGTARCCTTTAATAA 1.279 1.257 1.173 1.664 1.403 20 6153116791 2078355 6209 AAAGGCATCARTACAGTTCCC 0.158 0.154 1.552 1.4781.486 20 6 153135149 17710008 6210 TTACACCCACRGCCTCAACAG 0.348 0.3401.001 1.193 1.487 20 6 153140084 9384043 6211 CACATGATACYGGACAGCCAG1.837 1.743 0.691 1.054 1.447 20 6 153150872 1282450 6212GGGATCTAGGWGGGGAACAAG 0.134 0.122 0.751 0.745 1.058 20 6 153159957601240 6213 TTTCTCTATASCCTTCTTTTT 0.498 0.513 0.636 0.470 0.774 20 6153166436 3823082 6214 GATAACTAAAYAAAATGGTTC 0.263 0.266 0.128 0.5740.649 20 6 153172175 688136 6215 TGAAGGAAAAYGATACGCAAC 0.071 0.075 0.2460.457 0.345 20 6 153183689 311347 6216 TTTTTCATGTRTAATCCCAAA 0.543 0.5080.226 0.069 0.177 20 6 153199759 610111 6217 AGTAATGCTCWTTCCTGCTAT 0.4670.489 0.137 0.097 0.182 20 6 153205685 311353 6218 ATATATTGGCYATCTACATTG0.446 0.454 0.136 0.049 0.169 20 6 153213019 — 6219GCTGTATAGTRGCAAAGCTTC 0.000 0.000 0.094 0.048 0.019 20 6 153219459625764 6220 TCGTTTGATGRCTTTAACAAA 0.121 0.111 0.029 0.059 0.020 20 6153227029 628410 6221 GGTCATTTTARAGACTATTAT 0.278 0.280 0.021 0.0270.070 20 6 153235164 1328395 6222 GCTCCTCTCTYCTCAAAGGGA 0.034 0.0350.047 0.009 0.081 20 6 153245407 9371268 6223 TGTCGATGTTYTGAATCACAT0.381 0.376 0.052 0.004 0.112 20 6 153255860 1154023 6224AGAAAGTTTCRAAGGGGATTT 0.292 0.258 0.023 0.106 0.141 20 6 1532661139384046 6225 CCAACACCATYGGCTCTTCTG 0.098 0.101 0.036 0.172 0.185 20 6153272074 17083127 6226 ATGGGAGCATYTTATAATGTG 0.059 0.061 0.250 0.1940.165 20 6 153277784 9371269 6227 GCTAGAATGCYATGGTGGATG 0.146 0.1500.356 0.371 0.199 20 6 153283388 17083144 6228 AAATAGCCCAYGCAATTACAA1.280 1.287 0.507 0.495 0.246 21 7 30326902 38433 6229GATTACTGCAWCAATTCCTAG 0.133 0.139 0.160 0.668 0.348 21 7 30332916 384376230 ACCAATCCCGYGGACAATAAG 0.039 0.042 0.171 0.454 0.470 21 7 3034402438450 6231 GCTCAAATATAMCTTCATTAA 0.633 0.654 0.488 0.262 0.396 21 730359460 38466 6232 CTATCATTAAWCTCTCCTTAA 0.307 0.300 0.558 0.239 0.46121 7 30363462 17159202 6233 AAAATTGAAAYCCATAATGAA 1.250 1.281 0.5580.338 0.348 21 7 30378807 10227389 6234 ATGGGATGAGYTCTCTCAGAC 0.3400.363 0.369 0.347 0.272 21 7 30398623 10243040 6235CAAGTCTCTTSATGCCTCTTC 0.075 0.077 0.582 0.472 0.341 21 7 3040965617159267 6236 CTATGTCAGCYGAAAAATAAT 0.241 0.242 0.183 0.425 0.364 21 730417902 — 6237 ATGCATGTACRGCFGTCAATA 0.740 0.760 0.240 0.480 0.427 21 730427347 6945035 6238 TTATATGGGCRCAGAAGATGT 0.183 0.185 0.375 0.3090.481 21 7 30435477 10249885 6239 TGGCATTATARCCTGAAAAAA 0.541 0.5150.457 0.329 0.464 21 7 30444327 14270 6240 CTGCTGTAGTYGCTCCATTCA 0.4820.466 0.416 0.448 0.430 21 7 30462973 17159316 6241GGTTAAACAGRGACCCTAAGG 0.000 0.000 0.569 0.467 0.175 21 7 304670154722999 6242 TGGCTCTACCYGGTGCCATTT 0.641 0.664 0.592 0.393 0.243 21 730481779 2008003 6243 CCCAGGTGGGMTCTAATTTGA 0.000 0.000 0.457 0.3790.625 21 7 30489883 2267716 6244 AGAAGGAAAAYGGACCATGGC 0.000 0.000 0.5060.228 0.885 21 7 30502912 17159371 6245 TCTCCTAAAAMATCATCAGAG 0.3160.314 0.233 0.316 1.563 21 7 30505347 255105 6246 AACTGGACATYGGAGCGGAAT0.568 0.568 0.124 0.753 1.616 21 7 30520933 255131 6247TACGTTCTTASGGATCCAGCC 0.203 0.153 0.249 0.851 1.951 21 7 30535092 2551526248 TAATACCATCRTCAGAAATCC 0.014 0.014 0.789 1.651 2.035 21 7 30540960255160 6249 ATGAAAGCTGYCTTGCCTAAG 0.768 0.722 0.940 1.519 1.981 21 730552965 17159410 6250 CTCACCTGGARAGAAAAGCTG 1.693 1.726 2.390 2.0362.968 21 7 30565088 4723010 6251 CTACAGCTTCRATGGCAGCCC 0.858 0.873 2.5902.270 3.481 21 7 30579153 3901848 6252 TGTCCTTCCARTGTAAAGTCC 2.165 2.2302.426 2.351 3.358 21 7 30592754 6949578 6253 TTGAAAACTAYGCAAGTACAT 0.3030.302 2.012 3.511 3.905 21 7 30602279 11768076 6254TGTGGCCTTTRAGTACCTTGG 1.690 1.617 1.882 3.988 4.153 21 7 306107877803974 6255 AGCAGGGCTAYGTTCTGAGAA 1.004 1.027 2.837 3.542 4.312 21 730618224 1990011 6256 TGGATATGTCSAGTGGAGCTT 0.552 0.565 3.835 3.8354.312 21 7 30629046 10237363 6257 CCCGGGTCTCRTATGCCAAAT 3.996 3.5763.496 3.775 4.422 21 7 30641902 17159487 6258 GTAAGTTAACKAAGGTCTCCA2.330 2.254 3.676 4.153 4.312 21 7 30649882 10229281 6259TGGGAATAGARCTGCTTCATC 1.197 1.159 3.511 3.723 4.225 21 7 3067193717159526 6260 TCAGGTTGTTSCAATGTCCCA 3.110 2.896 3.415 3.710 3.988 21 730682866 17159567 6261 CAAAGTTATAMATCTCTTAAA 0.327 0.340 3.019 3.6873.748 21 7 30688503 10216063 6262 GCATTACAACRTGTGCCAGGT 2.161 2.8942.797 3.102 3.163 21 7 30701508 10281903 6263 CAATCGGGGARCGATTCGATT0.547 0.570 1.020 2.687 3.085 21 7 30710418 1000597 6264AGGACAGTTTYGTAATCAGGA 0.175 0.183 0.932 2.397 3.031 21 7 30719606 7634226265 ACTGGTGGCCYGCTAAATCCG 0.237 0.244 0.408 0.943 2.669 21 7 30732804765839 6266 TCAGCAGTCASGAAGGCACAT 0.218 0.220 0.432 0.999 2.127 21 730745035 10244884 6267 TTGGAGCTATYGACTGATTTT 1.090 1.087 0.464 0.3311.819 21 7 30762253 7801005 6268 GCCAGGCTAAYAAGGTCTCAG 0.630 0.630 0.5720.631 0.767 21 7 30768151 4723034 6269 TAGACAAAATKTTAAAGTTAA 0.221 0.2220.534 0.698 0.805 21 7 30775780 17159769 6270 ATGATGTGATYGGGCTGTAAA0.525 0.509 0.757 0.615 0.356 21 7 30776153 17159772 6271CCCCTTTTGCYGTGAGAGTTT 0.123 0.126 0.647 0.553 0.316 21 7 307903434988505 6272 AAGCTGAACCSGAATGTTTCC 1.552 1.558 0.557 0.377 0.397 21 730797413 11771444 6273 CGGAATGGGGYTGATCTCAGT 0.383 0.387 0.390 0.2960.460 21 7 30809401 6948808 6274 CCCATTCAATYCACAATAGCG 0.048 0.049 0.5140.330 0.429 21 7 30818744 7786414 6275 TCCGAACGTAYCTTTGGTGAA 0.026 0.0260.098 0.362 0.281 21 7 30827485 2391936 6276 TATATATAGAYTGTATGTTAC 0.0000.000 0.067 0.469 0.283 21 7 30838336 — 6277 TAAATGCACAYATAAAATCAT 0.3190.307 0.193 0.126 0.347 21 7 30848961 17159844 6278TGCTGGACATYTCACTGTTAC 0.328 0.324 0.378 0.097 0.347 21 7 308584374439020 6279 GGACTTGAGCYAATTACATGA 0.643 0.584 0.246 0.182 0.457 21 730876872 17159871 6280 GTTTTGAGTTRAAGGGACGGC 0.562 0.565 0.253 0.3260.195 21 7 30880688 17723231 6281 CTAAGGACAGYGTATGGGATT 0.081 0.0800.357 0.348 0.218 21 7 30895706 2267729 6282 TCGGGCAGAGWTACCCATCCC 0.2770.279 0.356 0.465 0.288 21 7 30911743 2284225 6283 ATTTGCATCARGTCCAATCTG0.618 0.616 0.347 0.400 0.317 21 7 30921519 1541518 6284GGCCCTGCACKTAAGTGATTT 0.602 0.592 0.610 0.392 0.262 21 7 3093095810264624 6285 TGTTTCAAACYGAGCAAAACA 0.246 0.379 0.539 0.364 0.211 21 730936040 12672673 6286 ATTGTAAACASTAACATAACT 0.667 0.697 0.516 0.3770.168 21 7 30949204 7785534 6287 GCAAATAGGGRTAACAGCATT 0.100 0.106 0.4430.345 0.333 22 7 32696611 12154586 6288 CAAATGTTTAYTGAATACTAA 0.6340.642 0.815 0.953 0.829 22 7 32708674 — 6289 ATGTTTCCCAMAAAGACAATC 1.6631.571 1.446 0.883 0.575 22 7 32722241 7778110 6290 GTGATGAAGAYGTCCCTGCTA0.291 0.288 1.489 0.965 0.526 22 7 32734764 2278819 6291AACTTTACTTKCAGAAGAGGG 1.449 1.415 1.264 0.976 0.531 22 7 3274548517469596 6292 CAGGAGCTGARCAAACAACAT 0.572 0.551 0.697 0.971 0.604 22 732816185 16881929 6293 CTTCCACATCYGCTATTGAAG 0.122 0.122 0.596 0.6930.574 22 7 32829655 6942974 6294 ATTAGAAAACSTTATTCAAAT 0.000 0.000 0.1410.257 0.619 22 7 32838422 17385980 6295 CTTAATAAGGYTGTATCTTAA 0.1230.123 0.030 0.387 0.517 22 7 32847596 6462450 6296 ATTTATTAAGYGTGACAAAAT0.379 0.377 0.062 0.125 0.406 22 7 32855971 7796184 6297ACTTGATAAGYGGCAGAAATG 0.001 0.001 0.181 0.109 0.155 22 7 328625134723241 6298 GCTTAGAAAGWCATCAAAATT 0.293 0.279 0.206 0.100 0.164 22 732868656 10281012 6299 TCTCTTAGTCRAGTGCAACTA 0.755 0.734 0.217 0.0780.054 22 7 32870950 10256717 6300 AGAGAAAAACYGAAAATTTGC 0.243 0.2480.218 0.085 0.029 22 7 32891598 2392216 6301 GCCACCTGAGRCCCTCCCCAG 0.3940.419 0.186 0.084 0.065 22 7 32900239 17393789 6302GTATACTAAGYCAGCCATTTC 0.077 0.072 0.063 0.122 0.055 22 7 3290644711981778 6303 TTGTATTCCAMGGCCAAGAAT 0.153 0.153 0.094 0.087 0.094 22 732914544 6964595 6304 TACTAATCCCRGATCTGTTCC 0.196 0.175 0.073 0.0750.082 22 7 32944350 7796922 6305 TTTGAACACTRCATGCATTTG 0.442 0.440 0.0530.072 0.086 22 7 32954589 17169808 6306 CCCAAAGAAAYTGCCTTTTAT 0.2470.262 0.163 0.107 0.087 22 7 32961789 17169847 6307AAGTTTTCATRCTCTTTAGCA 0.057 0.056 0.198 0.121 0.060 22 7 3296905617169913 6308 TGGGTACAAGYATAGTGTGCG 0.686 0.687 0.228 0.102 0.058 22 733000206 759396 6309 TCAGTTACCCSAAATTTACCT 0.235 0.220 0.224 0.110 0.07422 7 33011440 10486521 6310 TTCCTCAACARTCTGTCTGAA 0.627 0.583 0.2200.101 0.094 22 7 33017621 6979640 6311 AGGAAAAGAGSTGTATTGGGA 0.202 0.2010.085 0.094 0.100 22 7 33033399 1894872 6312 GTGTGCATAARCATAAAGGCT 0.0390.039 0.138 0.196 0.222 22 7 33051018 17170131 6313GCCAAGGTTCRATATACATGT 0.185 0.198 0.062 0.128 0.298 22 7 330572131420155 6314 GGAATCACTGYTTTACTGAGT 0.428 0.442 0.159 0.137 0.253 22 733063498 6952244 6315 GATCAATCTCYACATATTTCT 0.208 0.216 0.258 0.2530.268 22 7 33069447 17170142 6316 TTTCTATGCARTTTGATTTCT 0.624 0.6050.239 0.426 0.199 22 7 33076014 12671191 6317 CTATGTCTTGSAAAATTTAAA0.355 0.357 0.495 0.418 0.233 22 7 33081762 — 6318 TACAATATAAKGAGAAATATA0.135 0.143 0.801 0.393 0.166 22 7 33082095 7783612 6319TGTGACTTTTSATGATCTTTT 1.170 1.160 0.555 0.356 0.274 22 7 330951579791453 6320 TCAACCCTGAYATTTTTCTCT 0.959 0.914 0.467 0.406 0.346 22 733102971 17170169 6321 TAGGATTGAAWGTCGCAATAA 0.008 0.008 0.495 0.2720.341 22 7 33111593 17476738 6322 AACAGTTCAARTCAGAGGGAG 0.145 0.1440.228 0.432 0.296 22 7 33124869 10273634 6323 TTTTGTAATAYAAGGTTTCTT0.259 0.259 0.021 0.534 0.305 22 7 33141753 11771086 6324AATCCCACAARTATTTAATTT 0.000 0.000 0.246 0.221 0.227 22 7 3314776917170193 6325 ACAGGGTTGCRTGTCACTGAC 0.147 0.144 0.367 0.072 0.197 22 733154814 10230133 6326 TGTAGAAATCWGTGTAGAAAT 0.884 0.921 0.351 0.1290.180 22 7 33166726 10951387 6327 ATACTTGTCARATTAGAATTT 0.000 0.0000.255 0.143 0.089 22 7 33172356 17170203 6328 ACTCACTTTASGAATGGGACA0.235 0.234 0.345 0.128 0.127 22 7 33180964 3735420 6329ATATGTAACTSATATATTCAC 0.185 0.181 0.103 0.077 0.267 22 7 331845072109525 6330 ACAGGAGGGAKCGTATGTACA 0.319 0.311 0.066 0.136 0.343 22 733197001 17724206 6331 TGAGCTTATCRGAGGCCATTT 0.239 0.238 0.035 0.2140.343 22 7 33206555 1419898 6332 GCTCAAGTGCWTCTTTTTGGG 0.177 0.176 0.0800.324 0.276 22 7 33214153 6979138 6333 TTCCCACTTTYGTGATTCAAG 0.050 0.0490.351 0.400 0.342 22 7 33224797 1419928 6334 TACTCCTTATKGTAGTCATTT 0.5100.512 0.618 0.418 0.241 22 7 33232397 17170225 6335ATAAAATGTCRTGGAACCAGA 1.212 1.190 0.802 0.350 0.549 22 7 332392349639675 6336 TAACTTAATGMTTTTAAAGAG 0.890 0.835 0.908 0.409 0.544 22 733245933 17170234 6337 GTACCACATGYTAGGAGGCAC 0.575 0.545 0.700 0.4660.650 22 7 33251798 758779 6338 GTTTATCATAWTTGGTAGGTA 0.245 0.245 0.3661.114 0.755 22 7 33257747 1860601 6339 AGTTGAAAATKTTGCGAAAGC 0.081 0.0780.177 0.990 0.784 22 7 33271222 2392234 6340 TGTTCATTTGWTCCATAGATT 0.4700.434 0.803 0.832 0.799 22 7 33276768 9886325 6341 AATATTTTTCYGGATGTATCC0.322 0.349 0.766 0.811 0.768 22 7 33283598 4582453 6342TCACACACAARTTCACAAAGC 1.954 2.124 1.095 0.740 0.958 22 7 3329016011980608 6343 GGCAGTCACTWTGAAGAACTA 0.272 0.263 1.233 0.729 0.813 22 733297086 4723289 6344 TCAGGAAAATMTACAAAAAAG 0.722 0.712 1.145 0.7080.668 22 7 33303288 6964756 6345 TGTCATGTCCYTTTTTGTTTA 0.790 0.791 0.4571.047 0.554 22 7 33310906 11769528 6346 GCTTTTACCGMGTATTGTTTT 0.4070.385 0.372 1.052 0.842 22 7 33319060 2392242 6347 TTTTCAGGAAWGGATTATTTA0.244 0.255 0.661 0.500 0.954 22 7 33327456 17794801 6348ATGATTTACTRTAACATAAAA 0.029 0.027 0.510 0.437 0.882 22 7 333429656462483 6349 TTGTATTTTTRTTTAACAAAA 1.449 1.398 0.409 0.703 0.893 22 733353932 6944252 6350 TTCCATCTTCSTGCTCCCCTG 0.447 0.441 0.315 0.5460.420 22 7 33359477 2598408 6351 TTTACTGATAYTGAAATACTC 0.151 0.147 1.0010.435 0.516 22 7 33367994 13226813 6352 ATATAAACACWGTCTCAAATG 0.0210.022 0.482 0.492 0.392 22 7 33374179 1451018 6353 TGTCTTATTTYCTACTTCTTC1.566 1.532 0.321 0.516 0.325 22 7 33380156 2252989 6354TGTGCATATTSCATATATCTC 0.321 0.313 0.467 0.332 0.276 22 7 333915622598395 6355 TATGCTTTTAYGTTGCATTGC 0.048 0.048 0.493 0.264 0.284 22 733400828 1817055 6356 ATGGATCCTTYTTCATTCGTT 0.474 0.458 0.213 0.3320.601 22 7 33409604 17170308 6357 GTGTAGAAGTSCATACACAGA 0.073 0.0720.159 0.348 0.348 22 7 33414977 1451021 6358 AAGGGAATATRTGAGGCCATG 0.6990.682 0.256 0.112 0.444 22 7 33423634 7791607 6359 TGGGCTCAAAWGTAAGTACAC0.106 0.103 0.122 0.370 0.509 22 7 33440554 10486541 6360CCACAAAGCCRATAGTGATCA 0.439 0.430 0.183 0.381 0.510 22 7 3344650517170316 6361 ACGATCTCTGKAATAGCTTCC 0.100 0.099 0.535 0.496 0.279 22 733452874 1451011 6362 AGAGATAGGCRAGGGTAAGGT 0.287 0.277 0.540 0.6291.407 22 7 33459609 16879181 6363 GAGAAATCTTRCCATGTGGTG 1.724 1.6630.780 0.417 1.465 22 7 33466478 7811071 6364 CAGCATTTGTRAGGCCAGGTG 0.0870.088 1.071 0.497 1.778 22 7 33478731 1451010 6365 GGTTTTGTCAYCTGCTGGAAA0.949 0.998 0.929 2.997 1.890 22 7 33484293 4723309 6366TATCAGTTTTRTAAGTAAGCA 0.000 0.000 0.254 3.046 1.808 22 7 334917466966497 6367 CTTGTGGTGAYCAGCACTACT 0.049 0.047 3.852 3.474 1.969 22 733497620 17170353 6368 TACAACTTTCYTGCATGGAGA 0.366 0.349 2.886 2.2071.958 22 7 33505602 1376350 6369 AACTGAGAGCRATTGTGGCAA 5.035 4.267 2.7382.352 1.809 22 7 33506023 990080 6370 ATGCATCCACWACTTGATTCC 0.286 0.2882.775 1.879 2.037 22 7 33529577 7800309 6371 ACCCTTGGAARGATAAAAAGC 0.9080.905 2.823 1.707 1.427 22 7 33540418 2232121 6372 CAGATGTTGTRTGTTCTGGAA0.121 0.122 0.177 1.631 1.454 22 7 33549587 2042095 6373GGTTTTACAGRTAATGAGACA 0.208 0.199 0.293 1.891 1.239 22 7 3355758416879226 6374 TATATACCCTRGTGACTGGTA 0.131 0.131 0.071 0.226 1.130 22 733561770 2893475 6375 AAAATCTATTYGAGAGCCCAG 0.641 0.622 0.259 0.2301.171 22 7 33572120 4723328 6376 CTCCTTCCACYGCCACAGGAA 0.030 0.030 0.2440.135 1.164 22 7 33581291 12536852 6377 TATTTTTGTGYATTTAATTTT 0.8210.912 0.332 0.144 0.170 22 7 33587768 10951394 6378CAGTCTTTCCYTCATACTCTT 0.225 0.229 0.217 0.112 0.157 22 7 33593267 — 6379CTATCCTGGASAGCCTCCCTC 0.328 0.314 0.274 0.158 0.098 22 7 3360065310486618 6380 GTGTACCCTTSTTTTCTATGT 0.350 0.350 0.069 0.179 0.283 22 733607562 1362928 6381 TTTTTGGGTAYAGTTCCCAGG 0.220 0.220 0.098 0.2170.315 22 7 33613722 17170440 6382 ATCAAATCGTMAAAAAAAAAG 0.081 0.0820.210 0.120 0.393 22 7 33621606 7794034 6383 TAAAAGAAACRTGAAACCCCA 0.3760.392 0.184 0.418 0.554 22 7 33627530 17740751 6384GCTAATGAGGSCGAGTCATGT 0.777 0.740 0.217 0.446 0.556 22 7 336389817785398 6385 ATTTGGCCCCYATTAGTAACA 0.224 0.240 0.843 0.481 0.423 22 733645497 17740905 6386 GACAGCCTGGRCACTGGAGAG 0.315 0.313 0.853 0.8260.533 22 7 33652339 — 6387 ATAACAGTGTSTGACAAATGG 1.550 1.472 0.663 0.8140.474 22 7 33661735 10243554 6388 GCTGGTTCAGRCATTTTCTCT 0.365 0.3561.120 0.775 0.589 23 7 127239803 17151653 6389 TTATTTGAGGRGCTCCCCACC0.252 0.252 0.112 0.071 0.173 23 7 127254714 6467157 6390TCGGAGTGGAYGTGGACTTAC 0.554 0.538 0.133 0.084 0.416 23 7 127262797 —6391 CTGTCCAGTGKGCCCCATGTG 0.239 0.225 0.167 0.191 0.310 23 7 1272825766962635 6392 GGAAAAACACRCAGCCTGTTT 0.173 0.174 0.248 0.266 0.320 23 7127289004 6966724 6393 AATAGGATGGRTATCTGCCCG 0.359 0.347 0.321 0.7530.240 23 7 127296399 17151707 6394 GAGGCGTGAGRACAAAAGGAA 0.570 0.5260.424 0.705 0.315 23 7 127300637 3757754 6395 GCATTGTTAAYGTTCATCATC0.780 0.778 1.294 0.600 0.318 23 7 127317264 124756 6396TTGGCAGGAGRCAAAGTCCTG 0.000 0.000 1.099 0.553 0.283 23 7 127325849 —6397 GATCACAGAAYACCTGAATGC 1.966 1.896 1.001 0.673 0.390 23 7 1273336726954161 6398 CCTCAGAGATKTCACAGTAGA 0.010 0.010 0.615 0.586 0.276 23 7127360351 10258271 6399 TATCTTCTTGSACCCAATATA 0.208 0.199 0.616 0.4140.256 23 7 127377349 322740 6400 ACAGCATTAGKTTTGAAAAAT 0.081 0.079 0.0330.401 0.321 23 7 127383147 53125 6401 CTGAAACGCASTTTTTAAGAT 0.557 0.5160.032 0.289 0.285 23 7 127389755 10447854 6402 ATATAATTGCYGGGGACATAC0.078 0.081 0.144 0.016 1.220 23 7 127396223 10954170 6403AATAAATCACMAAGTATGAGT 0.037 0.037 0.132 0.049 1.073 23 7 12740830817293437 6404 ACAAAAAATARTAACAGCAGC 0.753 0.742 0.043 0.047 1.047 23 7127423722 896178 6405 GGCCCAGACGMTGCCATCATT 0.070 0.066 0.120 1.2280.636 23 7 127435720 — 6406 TGTTTATTACKTTGGACCGCA 0.070 0.070 0.1551.065 0.682 23 7 127442525 2060736 6407 TAACCGCGCCSTTCATTCTGT 0.5230.510 1.803 1.211 0.624 23 7 127451999 11764840 6408CTTAGTTGCAWTCCGATATGC 0.160 0.140 2.105 1.399 0.707 23 7 1274645791376349 6409 TGGTTTAAGCYTTTGAGCTCT 4.300 4.364 2.069 1.113 0.737 23 7127476021 4731426 6410 TGCTAAATACSCGCTGTTGGC 0.114 0.113 1.976 1.1830.862 23 7 127484013 11763517 6411 GTGGCCATTAYTTGAGAGTGA 0.469 0.4651.753 1.197 0.976 23 7 127493080 2060715 6412 GCTAAGTGACRGATACATGGG0.442 0.460 0.093 1.284 0.965 23 7 127499508 10954175 6413CCAGGTGAAARGCCCAGCGCT 0.199 0.203 0.153 1.353 0.948 23 7 12750718210274395 6414 CTACCCCATTSTGCAGTGAAA 0.000 0.000 0.189 0.233 0.924 23 7127517729 7811892 6415 GGACTTGCTTYAGCCTTACAC 0.345 0.353 0.239 0.3570.912 23 7 127523017 12534540 6416 CATGTTAAAARTTCAGAGGAA 0.642 0.6010.351 0.292 1.042 23 7 127542555 12538391 6417 CCAAGTCATGYCTCGAGGTGA0.601 0.566 0.587 0.199 0.215 23 7 127550209 4731441 6418AGAGATGCATWCACAAATAAA 0.461 0.481 0.510 0.252 0.239 23 7 12755678612536858 6419 CTAGGTGTTCRCTACCACAAA 0.710 0.707 0.299 0.417 0.176 23 7127567084 359650 6420 GAAATCTAAAYGAAAACGTGA 0.133 0.133 0.240 0.4010.162 23 7 127581730 962294 6421 AAATCTAACTSTGTCTCTATG 0.034 0.033 0.3130.307 0.149 23 7 127618814 885343 6422 CAGCGTAGAGYCAACCCTCCA 0.429 0.4420.181 0.175 0.171 23 7 127667809 17152065 6423 CTTACTATGCRTCAGACACCA0.773 0.744 0.229 0.155 0.173 23 7 127678648 13222212 6424ATTTTGGGTARAATCCCCTTT 0.306 0.312 0.245 0.067 0.135 23 7 12768424917152085 6425 CTAAAAATAGYTTTGAGTAGT 0.298 0.312 0.225 0.075 0.138 23 7127704972 — 6426 GGATGGCAAARTGTTTTATTT 0.049 0.044 0.059 0.129 0.158 237 127721306 2562737 6427 TCACTCAAATWATGAGCCCAA 0.397 0.396 0.048 0.1100.178 24 7 145001785 6943520 6428 TTTATAATGAYGTTTTTACCA 0.556 0.5550.384 0.307 0.172 24 7 145018059 4601231 6429 TCTTTTCTGAKACTACGGAAG0.639 0.617 0.519 0.228 0.139 24 7 145034566 10321215 6430CTCTCTCTTAMGCTCTAATGA 0.118 0.119 0.317 0.212 0.283 24 7 1450466774392813 6431 ATCTCCCACTRTCTCCATTTG 0.517 0.514 0.157 0.231 0.264 24 7145055277 7780753 6432 CCCAGGTCCARTTGGGGAAAT 0.134 0.144 0.070 0.1290.250 24 7 145071042 4355696 6433 AAAATGTTAARGGGAATAAAA 0.068 0.0650.102 0.299 0.268 24 7 145083737 7799145 6434 TATAGTTATCYGCAACTTACT0.333 0.342 0.044 0.186 0.261 24 7 145096619 7786054 6435GTAATACGTGYGTAGTGGTAT 0.251 0.250 0.384 0.223 0.184 24 7 1451145307777578 6436 AGTGAGATTTKGTAGTGTTTC 0.176 0.175 0.399 0.212 0.143 24 7145126258 — 6437 CCATTCCAAGSAAACTCCATT 1.397 1.355 0.369 0.199 0.094 247 145137729 6968866 6438 ACCACAAGTCYGATAAGGCTT 0.069 0.070 0.473 0.2400.119 24 7 145139853 10226110 6439 GGTGGCCACAYTGTACTAGAC 0.274 0.2650.440 0.207 0.090 24 7 145155154 17169920 6440 TGAAGTTTTAYACACAGTTCT0.482 0.496 0.102 0.192 0.096 24 7 145164360 17169926 6441GTCACTCAGGKTTATTACGCT 0.144 0.142 0.115 0.244 0.093 24 7 1451688124726779 6442 ATAGCCAAGCYTCATTTTCCT 0.270 0.275 0.104 0.054 0.066 24 7145205736 10277243 6443 TGCTTGCGTTRTTATCAGTGT 0.208 0.210 0.064 0.0680.110 24 7 145211439 2204466 6444 CACAAGGAATRGCAAGAAGAG 0.240 0.2320.103 0.049 0.223 24 7 145222414 4726781 6445 TTTTACTGCAWTATTAACTAT0.000 0.000 0.111 0.014 0.062 24 7 145231727 4726782 6446GATGACTTACYTAACAGATAT 0.328 0.318 0.078 0.065 0.080 24 7 1452419506464728 6447 AAAGCACATTYGTAGATTATA 0.280 0.276 0.046 0.194 0.300 24 7145245095 2692359 6448 CCATAATATTYCTCTAGTTCC 0.012 0.011 0.146 0.1730.288 24 7 145261967 1260126 6449 ATCTATGCTGWTTTAGAGGAA 0.001 0.0010.340 0.209 0.770 24 7 145291239 7807672 6450 TACAACTTTCRCCTATGCTAA0.681 0.700 0.240 0.533 0.785 24 7 145307229 1405115 6451AATTTTATTGWATTTATTCTC 1.030 1.017 0.358 0.547 0.841 24 7 1453196494612252 6452 ATTATTTTCTRTTTTACAAGT 0.038 0.039 1.133 1.141 0.894 24 7145332263 7795190 6453 TTTTTGTTCCMTGATACTATT 0.367 0.368 0.964 1.2770.780 24 7 145345572 6464738 6454 AGACAAGACTRCTTCATTTTT 1.770 1.7301.512 1.435 0.835 24 7 145351794 802539 6455 ATTTTTAGGARCTTTCATTTT 0.3950.384 1.882 1.534 0.814 24 7 145359146 2693307 6456AGCAAGTTACRTTTTTATCAA 2.407 2.405 2.323 1.184 0.857 24 7 1453660771526079 6457 CGCAGGAGTGMACCTGTTTTC 0.338 0.345 1.453 1.518 0.890 24 7145372744 17170000 6458 GTTCCAAAAGSAAGCTTATGT 0.000 0.000 1.235 1.3650.748 24 7 145385679 802522 6459 AGTTTAGTGARCTCAAGGTGA 0.000 0.000 0.1150.632 0.657 24 7 145390594 2430327 6460 TCCTGGAATTMATCATGAGAC 0.0410.041 0.012 0.599 0.887 24 7 145411268 6946638 6461AAGCACAATTYGAAGCACTGG 0.000 0.000 0.012 0.008 0.903 24 7 145419484 —6462 TCAAAGCATGYAGTGAAGGGA 0.077 0.072 0.015 0.042 0.677 24 7 14543132610262823 6463 TCATGATGTTRAGGGAAATAT 0.157 0.159 0.014 0.145 0.730 24 7145450428 7795585 6464 ATACATTTGTWTTTTTTCAAT 0.243 0.251 0.080 0.1390.381 24 7 145460671 17170038 6465 CACAAACTGARCTTACTTTAC 0.059 0.0570.342 0.368 0.377 24 7 145476714 10248899 6466 AGCCTTCATTMTTAATGTTTG0.707 0.705 0.405 0.388 0.474 24 7 145489102 17170058 6467AGTCTAATTAYTATTGTAGAC 0.915 0.923 0.696 0.630 0.474 24 7 14550055712703803 6468 GTCTATTAACKTAATAAGCCA 0.351 0.364 0.892 0.689 0.536 24 7145512007 17170073 6469 GGAGGATTTTYGCCTATAAAA 0.959 0.940 1.007 0.8680.632 24 7 145520605 10238194 6470 TTGTATTACARCTGTTTTAAA 0.534 0.5290.787 1.048 0.801 24 7 145528627 13247443 6471 GTAGCCTCCAWATTTTGTTCA0.893 0.940 1.049 0.912 0.930 24 7 145535097 1358079 6472TGTTCTCGACYTTGTTTCTGC 0.338 0.339 0.872 0.892 0.977 24 7 145541459802194 6473 TCTGGGGACTRCCTTGAGTCA 0.879 0.875 0.737 1.082 1.096 24 7145553353 1724513 6474 TTATTAACTAYTCAAATACTC 0.000 0.000 0.742 0.9531.123 24 7 145557979 1718093 6475 ATTGAACTTTSTGTCTTTTAA 0.304 0.3270.949 0.956 0.987 24 7 145568439 1724497 6476 ATGGTCCCTCRTCACGCTGTG0.913 0.981 0.845 0.911 0.954 24 7 145579861 17505462 6477GTCACCAAAAYTTTTATCCAG 0.792 0.827 0.860 1.071 0.766 24 7 1455844711639470 6478 GCATATTTCAYGAATGCCCCT 0.721 0.767 1.011 0.898 0.764 24 7145597926 7805539 6479 ATACAAGAAGRGAACTGTTAT 0.620 0.633 1.102 0.7410.639 24 7 145606854 6968491 6480 CAAATGCACCRTTACATAGGC 0.642 0.6610.775 0.666 0.603 24 7 145611435 17170116 6481 TGATTTGGTTYAAATAAGACT0.951 1.005 0.466 0.572 0.421 24 7 145625916 — 6482AGTTTCCATGYACAACTAAAT 0.183 0.177 0.276 0.437 0.369 24 7 14563227710227262 6483 CTACAGTGTAYTTCACCCAAA 0.031 0.031 0.276 0.279 0.356 24 7145656868 17584924 6484 CGTCATTCTTMCTTAAAAGCA 0.096 0.095 0.100 0.1640.201 24 7 145664230 17170134 6485 CAAGAACATTKATGATCCCTT 0.597 0.6040.079 0.093 0.152 24 7 145677193 2693406 6486 GCAGAAACCAYCTAGACTTGT0.295 0.294 0.071 0.026 0.078 24 7 145684853 2693392 6487TTGGATTGATKAAACTTAGGG 0.153 0.119 0.091 0.018 0.049 24 7 145691340802013 6488 AAATACTAGGRCAGTTAAGAA 0.042 0.041 0.037 0.028 0.029 24 7145697862 10249254 6489 GTGAACFGATWAACTTTGCAG 0.176 0.174 0.014 0.0250.039 24 7 145704173 10249436 6490 TCACATTTCAMACTTTATCTT 0.291 0.2850.020 0.006 0.042 24 7 145710348 10229049 6491 AAACAGGAGTKGTGACTTTTC0.002 0.002 0.015 0.005 0.090 24 7 145717706 6960544 6492ATATATAATASTCATTAATAG 0.000 0.000 0.009 0.084 0.090 24 7 1457251997789059 6493 ATCAAAGTCAMTAGAATATGA 0.063 0.060 0.002 0.092 0.068 24 7145734775 13247654 6494 TCTAACATTTRTCTTTGAGAG 0.117 0.113 0.352 0.1890.074 24 7 145741781 347195 6495 AATACTATTTRTCTTATTTAT 0.000 0.000 0.2620.161 0.155 24 7 145747802 17170192 6496 CAAGCTAATTKTCTTCCTTAT 1.0641.073 0.494 0.261 0.181 24 7 145754722 347223 6497 TGTAAACTTAYGTTCTCACAA0.121 0.115 0.492 0.261 0.188 24 7 145761651 6968860 6498ATTGGGTCATMTCTAATATAA 0.732 0.724 0.492 0.453 0.215 24 7 14576873913229953 6499 GGAAGAGTACMCTATGGGCCA 0.091 0.091 0.215 0.535 0.247 24 7145775759 13235730 6500 CCTTCCAATASTTTGCAATTG 0.000 0.000 0.517 0.4590.388 24 7 145781202 2215327 6501 TATAGTAACARTAATGTATAA 0.000 0.0000.305 0.265 0.681 24 7 145790224 10277654 6502 TGCCTGAAATYATGCCTTGTT0.729 0.726 0.390 0.273 0.700 24 7 145800130 2372761 6503CTGTTTTCAARTTATAATTTC 0.345 0.365 0.360 0.408 0.714 24 7 1458088051494459 6504 GTCACCAAATSGTAGTTGTTT 0.226 0.231 0.282 0.915 0.508 24 7145826907 2642483 6505 GTCTTCTACAYTTCCTTTTGT 0.382 0.360 0.453 0.7690.616 24 7 145839399 344453 6506 TGTGAGGTCARTTGGTTAACC 0.191 0.195 0.9320.790 0.699 24 7 145845612 17170221 6507 CATATATATCYGGTCTTGGCA 1.2191.199 0.919 0.600 0.716 24 7 145851145 344470 6508 CTTGAGACCCYAAAACAGTAT1.543 1.548 0.979 0.636 0.885 24 7 145858219 344465 6509TAGACTTACTRAAATTATACC 0.095 0.092 0.965 0.910 0.801 24 7 1458651487811006 6510 ATCCCATTTTRTAACATTGTA 0.560 0.516 0.622 0.812 0.743 24 7145879656 — 6511 ATCCTCTAAGKAGAATATTTG 0.151 0.190 0.379 1.134 0.724 247 145884415 6956550 6512 CCCTGAATGARTGAAGCTATA 0.490 0.468 0.463 0.7830.757 24 7 145895515 10952659 6513 TAGAACATTCSTCCAATAATG 0.968 0.9790.665 0.419 0.677 24 7 145902613 10952661 6514 ATTAAACAAGYTGCCCCAATT0.262 0.258 0.700 0.525 0.706 24 7 145918045 11766778 6515AATATATTCTWTCTTTTAAAT 0.708 1.157 0.750 0.485 0.432 24 7 1459274931541512 6516 AGTGTCCCCARCCATAAATTT 0.274 0.257 0.422 0.470 0.149 24 7145943250 — 6517 AGGAATCACAYTTTTAAAAAT 0.000 0.000 0.485 0.393 0.200 247 145962677 10234284 6518 TTTTTATTGCSTTTATTTTTC 0.340 0.340 0.113 0.1550.350 24 7 145970176 10282480 6519 GAACTGTATAYCTAACAGGTA 0.368 0.3660.106 0.103 0.628 24 7 145983299 7781516 6520 CTAGGAAAGTSGTCTTATTCA0.050 0.042 0.071 0.038 0.569 24 7 145984741 6955151 6521ATACTGTTTTYCAGGTAGCTA 0.262 0.264 0.026 0.222 0.383 24 7 1459971224367451 6522 GGATCATAACRTGCTATTTCC 0.110 0.112 0.032 0.491 0.562 24 7146008270 17170284 6523 AACTGGAAGTSCATGCGATTC 0.026 0.026 0.373 0.4410.420 24 7 146015977 17170287 6524 TGTATTGGTTKTCTCAAGAAT 0.415 0.4220.882 0.393 0.403 24 7 146022793 17483333 6525 AGCCAATGAAWTAATCTATCA1.347 1.278 0.877 0.670 0.342 24 7 146029536 10216156 6526GCTACCATACRATCGATATCC 1.466 1.411 1.036 0.756 0.296 24 7 14603496110500168 6527 TAGTGTTGTCYTGTAGCATAG 0.177 0.178 1.204 0.718 0.344 24 7146047361 11765622 6528 ATCCCCTTTARACCTCAAGAA 0.064 0.203 0.860 0.7410.431 24 7 146059994 11773694 6529 AATCGCACCTKTATTTCTGGC 0.979 0.9870.315 0.623 0.465 24 7 146074532 2049396 6530 CTACAAAAAAWCTAAAGGAAT0.396 0.431 0.300 0.460 0.632 24 7 146083998 — 6531ATAAATGAAAYTGTCAAGATA 0.135 0.135 0.273 0.284 0.865 24 7 14609058013228798 6532 GATAGGTTTGYCATCACAGAG 0.116 0.114 0.134 0.330 0.868 24 7146096763 12703865 6533 CCAGTTACGCRGTGGTTACTT 0.020 0.020 0.201 0.5300.758 24 7 146112499 1524340 6534 AACTGAAAACKGCAATTGTCA 0.555 0.5340.275 0.641 0.611 24 7 146119599 12703874 6535 ATGCCGTCAGSTAAACATAAT0.522 0.521 0.601 0.646 0.601 24 7 146126667 13234249 6536ATCATAAATGYATTGTTACTG 0.418 0.383 1.073 0.743 0.582 24 7 14613980210281702 6537 TTGGTCTCTCYGGAGGCAGGA 1.230 1.198 1.163 0.876 0.445 24 7146148505 17170304 6538 CTATACTTCTSCAATACAATT 1.566 1.522 1.301 0.9360.439 24 7 146165900 1524347 6539 TATTTAACATRTCACAAAATG 0.000 0.0001.508 0.833 0.406 24 7 146172529 982512 6540 TAGATATATAMATGTTAGACG 0.0000.000 0.926 0.797 0.507 24 7 146178225 7812091 6541CTCTCACTAAYGTTGAAATGT 0.000 0.000 0.056 0.792 0.655 24 7 14618957310266622 6542 TATCAGAATARTATCAGATCT 0.089 0.087 0.064 0.332 0.755 24 7146197132 6973868 6543 TTAGAGCAGARTATGCATGCA 0.180 0.175 0.093 0.0940.646 24 7 146204462 6975159 6544 CTCAAAGTTGYTTACTTACTA 0.309 0.3030.045 0.147 0.561 24 7 146215148 10952669 6545 CAGGTGTATARCATGAACTTA0.423 0.413 0.156 0.246 0.397 24 7 146224977 — 6546TCAAATCAATYAGACAAATAC 0.016 0.043 0.306 0.174 0.147 24 7 14623078111973305 6547 TATTGATTTASCATTAATCAT 0.618 0.624 0.525 0.188 0.114 24 7146238152 13238590 6548 GTGTGTGAAAYTTGCAAAATA 0.656 0.662 0.384 0.2790.084 24 7 146252578 6958777 6549 CACATTAAAGWTCTGATCTGA 0.778 0.8710.417 0.274 0.084 24 7 146269497 9640492 6550 AAACAGTTAAKTCAGGATTCT0.106 0.102 0.409 0.213 0.087 24 7 146275727 6959187 6551TATGATGACARCTGTGATAAT 0.112 0.112 0.283 0.232 0.078 24 7 14628253117170336 6552 CTACCTTTACWAACTTGCTCT 0.642 0.600 0.089 0.184 0.079 24 7146293204 1390718 6553 GAACACATATYGACTGTATTT 0.241 0.253 0.083 0.0970.084 24 7 146302372 1826843 6554 CAGCTGTCCTRTGATATCAGC 0.027 0.0270.122 0.024 0.102 24 7 146308562 4725706 6555 ACTCTGCACAYGTTGCTGCTA0.024 0.024 0.024 0.038 0.089 24 7 146313964 1496543 6556TAACAGCAAARGGTTCTTCAA 0.374 0.363 0.010 0.057 0.049 24 7 1463209507801686 6557 TTAATGCTTAYACTAAATTCT 0.072 0.072 0.035 0.024 0.011 24 7146327696 1018073 6558 GTGAACAGAAYTGTATTTTTG 0.066 0.063 0.080 0.0360.013 24 7 146373578 1390723 6559 CAATTGCTATRAAAGCATCAT 0.309 0.2870.056 0.033 0.050 24 7 146384563 — 6560 TCCGTGCTCTKTGCTGGAGGC 0.3950.383 0.132 0.021 0.031 24 7 146392045 9769600 6561ACAGAAGTCTRTTGAACAGGA 0.217 0.222 0.134 0.016 0.033 24 7 14639912717170379 6562 GTTTTTAGACYCTCCTCTGTG 0.477 0.456 0.067 0.100 0.042 24 7146405943 10279700 6563 GCTAAGTAAGYTGATATTCCT 0.089 0.090 0.044 0.1490.035 24 7 146413128 10268597 6564 AGTTGAGGACMATGTTTCAAA 0.028 0.0240.231 0.161 0.025 24 7 146435941 2620440 6565 TCTTAAGTAAYGAAAAGATTA0.174 0.176 0.212 0.146 0.036 24 7 146448938 4269450 6566ATCTAATGGGRAAATTTGAAG 1.071 1.148 0.266 0.102 0.059 24 7 1464562152692146 6567 AGCTTCTGAAWTAGGGATTTT 0.000 0.000 0.389 0.060 0.079 24 7146463556 16883333 6568 ACTCAGCTCTSTTGGTGCAGC 0.301 0.311 0.330 0.0740.109 24 7 146470362 747139 6569 AAACAGAGACKGAGGAAATCT 0.232 0.230 0.0410.160 0.081 24 7 146477116 16883356 6570 CAGCTATAAAMACATTTGCTT 0.0510.052 0.037 0.215 0.068 24 7 146487462 10254249 6571CTGCTCTATTKCCTGTTACTA 0.091 0.092 0.063 0.136 0.156 24 7 146496034826825 6572 GATTTTCTGAYAACAATGAAT 0.232 0.234 0.116 0.083 0.167 24 7146503811 13233234 6573 GGTCTATACAWCGGAAAAAAG 0.553 0.506 0.291 0.0700.236 24 7 146510232 11972997 6574 TCTGATAATTMCTCTTGCAAT 0.428 0.4260.231 0.182 0.128 24 7 146516306 1404709 6575 AAAAAGACTAYGTAAAAAATA0.577 0.589 0.233 0.200 0.116 24 7 146524144 700304 6576CCTTGTTGTCYACCGTTTGCT 0.015 0.014 0.386 0.303 0.142 24 7 1465337426963863 6577 ATAAGCCTCCRTTGAAAAGAT 0.190 0.183 0.252 0.296 0.179 24 7146542337 4726857 6578 CTGGTACTAAWCTACACAATG 0.950 0.951 0.273 0.2450.196 24 7 146555102 10251377 6579 AATTGGTGACRTTAAAGTTAA 0.030 0.0290.320 0.265 0.185 24 7 146562196 11773683 6580 ACAGAGGCAAMGGTGACTGAG0.676 0.678 0.344 0.249 0.258 24 7 146567619 17170498 6581TGCTTTCCTTYACTGGCACTT 0.178 0.188 0.210 0.267 0.247 24 7 146581895826641 6582 GCTTATTTCAWCCATGATGGA 0.304 0.298 0.402 0.242 0.185 24 7146593236 7779225 6583 AAAATGAAGARCTATCACTTA 0.568 0.566 0.218 0.1960.113 24 7 146600422 16883479 6584 TGGCTTGATGKGGTCAATTCC 0.589 0.5510.181 0.297 0.235 24 7 146610304 4726870 6585 AAGAAAATGGYCCTTTGTCTA0.172 0.163 0.343 0.165 0.532 24 7 146620791 2286127 6586TGTGAGTGCCRATTTATCTCA 0.056 0.057 0.296 0.136 0.423 24 7 1466273882141388 6587 AACTTACTTCYCCATCAGTTG 0.785 0.789 0.144 0.282 0.618 24 7146633513 1919190 6588 ATTTCAGTTCMCATTCAAAGT 0.455 0.430 0.128 0.5850.541 24 7 146640013 6970064 6589 ATCAATATTTRCCCAGTGCAA 0.035 0.0350.406 0.555 0.578 24 7 146646656 10282028 6590 CAGGTGCCAAYGTAAACCTAG0.089 0.083 0.756 0.767 0.925 24 7 146655654 2888493 6591GACTCTTCACRGATTTCTGGA 0.991 0.923 0.789 0.847 0.833 24 7 1466635291024528 6592 TATGTAAATCYGTTATAATAC 1.685 1.671 1.148 0.725 0.723 24 7146672375 12703926 6593 CAATATAAGCRTGACTTAATT 0.449 0.470 1.287 1.1490.779 24 7 146678432 4118165 6594 ATGTAATAAGSCAGTAATTGA 0.826 0.8321.013 1.163 0.838 24 7 146684627 3915304 6595 CATTATATTTSAATTATTTGG0.378 0.368 0.997 1.191 0.722 24 7 146690849 6464821 6596GGTGTTTCGTRTTCTACTGGT 0.403 0.417 0.882 1.049 0.744 24 7 1466973762074712 6597 CTTTTAGATCRTGACAGACCT 1.701 1.626 0.664 0.664 0.925 24 7146711455 2189998 6598 TCTTCCACCARTGAAAAGTCA 0.200 0.193 0.723 0.6141.080 24 7 146719444 2158640 6599 CCACTATTACYGATGTGAAAA 0.270 0.2700.694 0.549 1.385 24 7 146733806 7794693 6600 CTAAAATAAAMTGTCCCAATA0.510 0.496 0.263 0.699 1.063 24 7 146741320 7807559 6601TATTTATTGASCACTAGAGTA 0.394 0.395 0.301 0.749 1.098 24 7 146748760988039 6602 ATAAATATCTRACAACAGGTT 0.287 0.292 0.546 0.874 1.208 24 7146751457 727716 6603 ATAAGTAAGTRAAATAAACGT 0.000 0.000 0.585 0.9711.277 24 7 146773166 17170626 6604 AGGTTCTTAGWGATGCTTCTA 0.903 0.9191.572 1.161 1.238 24 7 146788719 10244236 6605 GTATACCAAARGAAGCTTAAT0.568 0.573 1.488 1.339 0.954 24 7 146799052 17327617 6606ATCTCTGGCTRACAGGAAGAG 2.136 2.122 1.432 1.469 0.959 24 7 14680695517170638 6607 AGCCATGTTCYTAATACCCAA 0.305 0.311 1.410 1.384 1.055 24 7146815406 12672959 6608 CACTAAATATYAGCCATGGAC 0.525 0.534 1.573 1.3520.882 24 7 146819430 851735 6609 TCATGCTCTASTCTAAGCTTC 1.194 1.265 0.6631.071 0.772 24 7 146837806 17170645 6610 CACTTGAGGCRCATAGCAAGC 0.5320.518 0.800 1.041 0.811 24 7 146844658 700317 6611 TGTCCAACTARTTACTAAATT0.235 0.246 0.569 0.359 0.994 24 7 146852926 4295581 6612GGAAGCACAGWTGTATTAGCC 0.623 0.643 0.257 0.289 0.944 24 7 1468663614308646 6613 GTCTATTCTGMAGCCTGTGAA 0.023 0.023 0.103 0.251 0.843 24 7146880864 17417231 6614 TTTACCTTTAYGTGCTTCCTT 0.474 0.456 0.060 0.2530.521 24 7 146888752 10952713 6615 CCCTGCAGCCSTAAGTACCAC 0.010 0.0100.030 0.306 0.617 24 7 146899558 17823834 6616 ATCATATCTCYGCTTGGTGCT0.017 0.017 0.347 0.266 0.721 24 7 146926019 851697 6617AATCTATCAGYTCACTTTTCA 0.398 0.396 0.454 0.329 0.514 24 7 14693598817226520 6618 TTACTTCTTAKAAACGTGTAT 1.218 1.204 0.502 0.530 0.676 24 7146953728 17824995 6619 TTCTAGAGACRTTGAATCCAG 0.739 0.746 0.856 0.6770.638 24 7 146963031 17825363 6620 AAAATTGGATYGAAGTCCAGA 0.097 0.1011.017 0.820 0.890 24 7 146971654 17170691 6621 ATGTCTTAATYTTTCTCCTCT0.914 0.828 0.887 1.244 1.079 24 7 146975755 851705 6622TTCAAATTCARTTAGGCCTTC 0.694 0.710 0.762 1.152 1.008 24 7 14698757810488352 6623 CTGGTTGAAASCTAGACATTT 0.901 0.905 1.383 1.313 1.138 24 7146996863 6979528 6624 GCAAACCACTKCAAGGAAGCT 0.503 0.466 0.870 1.5091.209 24 7 147007736 10085572 6625 AAATATGAACWTTCAATAAAG 1.097 1.1251.448 1.411 1.595 24 7 147017267 2538991 6626 TGTCAACATTMCCCTTGCAGA0.065 0.069 1.360 1.212 1.305 24 7 147023830 10488350 6627GAGACACTGTYAAGTTAAATA 1.569 2.132 1.149 1.078 1.442 24 7 14702961217170738 6628 AAGCATTCGCYCTTGTGCTTC 0.787 0.782 0.796 1.393 1.587 24 7147035669 2710114 6629 GACTTCTACARTCAATTTGTA 0.090 0.097 0.977 1.3101.722 24 7 147047727 17237331 6630 TCTTCAGGGAYGCCTCCTGTA 0.544 0.5330.994 1.095 1.725 24 7 147055375 7805223 6631 TCAGCTCATGYCTGAGTCCCT0.364 0.354 0.612 1.321 1.691 24 7 147062628 17170749 6632TACCTACCGAYTATCAATTAA 1.685 1.713 0.947 1.063 1.611 24 7 1470697832710093 6633 ACGTCCTTTGSTCATACAGTT 0.055 0.054 0.898 1.080 1.705 24 7147075195 10808047 6634 CGATCAATACRATCTTCAAAC 0.796 0.765 1.276 1.3981.837 24 7 147084835 1882687 6635 GATAGCCCTGMAGAATCCTGG 0.302 0.4580.820 1.192 1.210 24 7 147093493 2708248 6636 AGGAGTCAGAYGTATATTCTA1.022 1.044 1.289 1.726 1.262 24 7 147100208 7805706 6637ATCAGCTATTSAACTTTTTCT 0.856 0.885 0.867 0.988 1.197 24 7 1471068887797584 6638 ATTTGTACTTMTATCTATGAG 0.596 0.856 1.481 1.113 1.331 24 7147113950 17170767 6639 AAAATGCTACSTGTAAAATTG 0.031 0.031 0.948 1.2121.204 24 7 147127273 6958434 6640 GGAATAGATGYCTAGTCCCAA 1.445 1.5430.596 0.985 0.778 24 7 147133198 17170777 6641 ATAGCAGGCTRCAGATACAGA0.124 0.112 0.758 0.962 0.858 24 7 147140340 2214681 6642GAGTGGCACCRAGAGGAATGT 0.285 0.296 0.756 0.675 0.659 24 7 14715304217170785 6643 ATAGTAATGGWCAAGCTCCCA 1.104 1.047 0.569 0.516 0.622 24 7147160642 993715 6644 ATTAAGATGCMTATGTGGCTA 0.085 0.085 0.526 0.5880.463 24 7 147166630 4726916 6645 TCACAATAAAYTCTCATTCCC 1.023 0.9960.503 0.218 0.616 24 7 147172614 2527059 6646 GGTGGGACAGYGATCCATGTT0.035 0.036 0.238 0.259 0.526 24 7 147178895 2707558 6647TCCGAGTTCAWATCTAACTTG 0.234 0.240 0.214 0.267 0.549 24 7 14718629617170811 6648 ACTGATTAGARTGTCAGAATA 0.326 0.324 0.048 0.398 0.327 24 7147192464 10485845 6649 TTGATAACCAYTGTATTTCTG 0.031 0.029 0.113 0.4640.377 24 7 147197109 10485844 6650 ACTTGAATTCRCAAAATACTC 0.330 0.3320.547 0.277 0.527 24 7 147208831 4726920 6651 GAGAATTTCCRTACTACCTCC0.391 0.377 0.572 0.318 0.418 24 7 147218722 10485843 6652CACTCCGTAGRTGACCATAGA 1.608 1.518 0.621 0.372 0.495 24 7 14723064417170828 6653 CTCTTTAAGCRTGTCAACTAG 0.361 0.352 0.592 0.573 0.491 24 7147237402 11974602 6654 CTGCTCAATAMATGTAGAAGG 0.159 0.163 0.638 0.6940.490 24 7 147243946 6962625 6655 ACTCTGCTAARTGTATTTCAT 0.255 0.2530.430 0.754 0.515 24 7 147254702 17170844 6656 CTGGATAAGARTAATTGAAAG0.000 0.000 0.497 0.959 0.567 24 7 147259047 10228263 6657ATGGCAAAGARATATTGTTCA 1.126 1.082 0.673 0.487 0.579 24 7 1472715331557964 6658 TCACTAAATCRTAACAAAAGT 0.502 0.505 1.029 0.484 0.570 24 7147284643 17170855 6659 GCAGAAACAGWAATTTCAGAA 0.501 0.533 0.803 0.6140.590 24 7 147295938 — 6660 TTTTGCTGCCKCTTCATGTCG 0.965 0.946 0.4470.556 0.345 24 7 147302259 2972108 6661 GTCAGACATCRGTGCAAAGAA 0.0200.021 0.453 0.489 0.333 24 7 147309435 12534650 6662ACACGCCAAAWTATTAACTGT 0.351 0.344 0.285 0.289 0.454 24 7 14731680210260743 6663 TGGCCAAGTGYGTGCCCTTTT 0.561 0.541 0.094 0.297 1.036 24 7147322254 2906313 6664 CTACGTAAAAYGAGCTTCTGA 0.134 0.123 0.194 0.2390.965 24 7 147323182 13230925 6665 GAGGAGATAGYTAAGATGACT 0.255 0.2650.256 0.173 0.770 24 7 147348080 4345484 6666 GTTCTGGCTTYGGGATGGAGT0.439 0.426 0.177 0.842 0.647 24 7 147356671 2373346 6667TAGTGTTTACRTCATGGTGTC 0.520 0.533 0.385 0.837 0.563 24 7 1473638851529154 6668 GCTGTCAGCCRATATGGGTCA 0.266 0.282 1.223 0.830 0.391 24 7147374780 4726933 6669 ATCGTTTCTGRAGCCTGATTT 0.687 0.665 1.183 0.7890.533 24 7 147380226 6945371 6670 ATATTCTGCGYGCTGCTCTTC 2.201 2.1321.250 0.795 0.481 24 7 147388963 4590355 6671 CATCAACGGCWCTTACCGCAC0.314 0.328 1.080 0.707 0.365 24 7 147399321 17170932 6672CATTTATTATYAGCAGGCAAC 0.000 0.000 0.939 0.736 1.916 24 7 14741160517170936 6673 AATGCACAAARCAGCTCACAT 0.061 0.062 0.100 0.661 2.239 24 7147414418 7809486 6674 GTCTTGGCTTRACTAGGACAT 0.245 0.239 0.186 0.4792.560 24 7 147425911 7779924 6675 TTCAGAGGCCRTACTGACTTC 0.256 0.2560.113 1.651 2.537 24 7 147437045 4726947 6676 TTTAGATGTASATTAAGACCC0.639 0.649 0.110 2.252 2.672 24 7 147464095 — 6677AGAAGTCCCCRGTGGCTCAGG 0.116 0.115 2.735 2.362 2.946 24 7 14747327010281587 6678 CTATCTCTTAYATTGCCCAGT 0.058 0.060 3.029 2.300 1.865 24 7147478836 11767934 6679 TTGTCAGATGSTCTTCACACA 4.904 4.364 3.526 2.7881.909 24 7 147485200 2037869 6680 TGAGGTCATGYGTAGTAAACA 1.174 1.1163.177 2.800 1.537 24 7 147491445 — 6681 TTTTAGAAGTRTGCTCATTTA 0.5420.499 3.124 2.516 1.863 24 7 147502534 1637864 6682ACATGTCACCRAGAGAAAATG 0.181 0.179 1.065 2.551 1.878 24 7 1475087661730398 6683 TATGTGACATRGAGCGTCAGT 1.382 1.369 0.585 2.251 1.993 24 7147515023 — 6684 TAACATACTTYACTTGTAATA 0.000 0.000 0.500 0.656 1.749 247 147524985 4726968 6685 TTTACCTAAAYTTGAGGAGAA 0.123 0.116 0.447 0.3081.775 24 7 147530631 10242598 6686 TTCCCCTGCCRCGAATTTCTT 0.376 0.3660.173 0.284 1.773 24 7 147541635 10215248 6687 TGCTTCAGTAYATAATCTAAA0.033 0.033 0.091 0.276 0.468 24 7 147555290 2530310 6688AGTTTATTTTYCAGATAAAAT 0.713 0.645 0.135 0.105 0.248 24 7 147567741 —6689 CCAAGGTTTAYGTAAAATAAG 0.076 0.075 0.066 0.086 0.305 24 7 1475733322530304 6690 TCTCTAAAAGMAATATAAAAT 0.000 0.000 0.263 0.184 0.298 24 7147581090 7780899 6691 GTGCTGCCACYGTCCTTTTGG 0.125 0.128 0.120 0.1390.442 24 7 147594288 17616888 6692 TTGACTGAAGRTAGGTTTCAT 0.617 0.5800.340 0.328 1.033 24 7 147600941 17171023 6693 TCTGAACCCCRATTCCATGGT0.215 0.224 0.259 0.188 1.752 24 7 147621152 6464905 6694CTTCAATAAAYGGGCAGGTAA 0.676 0.688 0.500 0.761 2.238 24 7 1476261816464907 6695 AAGGACTGCTYGACTATTTTA 0.206 0.207 0.305 1.533 2.855 24 7147635310 17617268 6696 TGCTGAAAAAKATTCCTTATA 0.787 0.787 1.024 2.3992.784 24 7 147649806 2538476 6697 ATTGAAGGCCYCTGGAAAGAG 0.141 0.1371.913 2.584 3.026 24 7 147659874 2717817 6698 TTCCATGAACYCTGCTCCAGT2.018 2.013 2.703 3.079 3.085 24 7 147665514 11766141 6699GAAGAACTTTSCAGAAGTAGT 2.854 3.488 3.021 3.159 3.139 24 7 14766558311769697 6700 AGTAGAACATRAGGTCATAAT 2.185 2.172 3.604 3.322 2.901 24 7147676361 7790220 6701 AAACTTATTTYAGTTATTGAT 2.142 2.515 3.604 3.4742.816 24 7 147695809 12704036 6702 TTGATACAGAYTAGAGCCACA 2.096 2.0443.364 3.623 2.835 24 7 147709488 11767479 6703 TTTTAAATCTKGCTATTTTAT0.479 0.453 2.979 3.386 2.872 24 7 147725167 6960877 6704ATTGTAGAGGYTTTAGATCAT 1.368 1.857 1.426 2.185 2.652 24 7 1477471066959357 6705 TCAAAATGTTRGAACTATACA 0.491 0.484 0.574 1.606 2.778 24 7147758038 1015665 6706 GGTTTCAGGAMAATATCAGAA 0.133 0.130 0.523 1.0252.143 24 7 147764071 12539045 6707 ACAGGTGCACRGCCCTGAGTC 0.252 0.2470.190 0.433 1.439 24 7 147770295 10952770 6708 AAATGAGAATYGCATAGTGCT0.274 0.272 0.253 0.339 1.115 24 7 147777033 17171069 6709TTTATTCTCCYAATATACTTA 0.000 0.000 0.193 0.109 1.088 24 7 1477906816963551 6710 GGATAGCCACRGTCTAAGCAT 0.657 0.652 0.163 0.113 0.777 24 7147803868 10278049 6711 GAAACAAGTGRGAGTCAGAGT 0.052 0.052 0.135 0.2090.807 25 7 149454345 11983355 6712 GGTGCCTTTGMCTTTGGGAAG 0.649 0.6220.167 0.479 1.283 25 7 149463563 11764936 6713 AAAATTTAGCKTTCCCAAGTC0.488 0.476 0.452 0.353 1.268 25 7 149471530 3735171 6714GGCGAGCACCRGCTGACCCCC 0.187 0.197 0.541 0.390 0.955 25 7 1494772033735167 6715 GTTCCCACACYGCAGGGATCA 0.949 0.925 0.552 1.199 1.331 25 7149483883 28432021 6716 GCTGTATTTTSAGAAGTACAA 0.315 0.309 0.568 1.3871.191 25 7 149497250 17173682 6717 TGAATAATTTYCACATGACTT 0.604 0.6042.173 1.247 1.272 25 7 149518638 2272187 6718 GGGGAGGACCYTGCCTCTTTC0.532 0.536 1.993 1.817 1.237 25 7 149530332 4725889 6719ACTTCTAACTYGTGGGTTCAT 3.092 2.972 2.101 2.510 2.111 25 7 1495456071048424 6720 ACATTGAGCCYCGAGTCAAGG 0.118 0.084 2.397 2.212 2.173 25 7149556724 2888635 6721 TGCATCGATCKGGTGAGACAC 0.000 0.000 2.430 1.9204.312 25 7 149571403 1524335 6722 TGATATACCAYGCAACATTAT 1.392 1.3170.750 2.335 4.364 25 7 149591461 7810209 6723 TTATAGTGGGYGATGTGTAAA0.600 0.592 0.638 2.414 4.312 25 7 149602928 2373748 6724TTCTTGCCTAWCCTATTTTAA 0.776 0.793 1.585 3.503 4.187 25 7 1496248932222524 6725 ACAATGGGTGRCTTTATTTAT 0.018 0.018 1.338 3.422 4.120 25 7149649666 7810752 6726 TGAAAATTCARCTTGTTCAAG 2.307 2.313 4.665 3.5854.153 25 7 149660076 7803234 6727 TTTTGTTTTTRTTATTTGTTA 0.689 0.6814.488 3.447 3.623 25 7 149706735 13242186 6728 ATCTCTGAGTSCAGCAGTAAC4.565 4.789 4.665 4.966 3.133 25 7 149727819 4358715 6729TCTGCCTCGTWTGGTTTCTGC 0.101 0.100 3.206 3.748 2.656 25 7 1497380964367453 6730 TTAAAAAGGTYATTCCTACCC 0.000 0.000 4.036 5.267 2.852 25 7149749542 — 6731 TCTGTTTTTCYCCAGCAGAAG 0.252 0.261 0.254 2.218 3.143 257 149756449 10253121 6732 ATGTGACAGTYTCTAGTTCCC 0.987 1.030 0.559 1.7212.630 25 7 149763839 10272462 6733 AGGAATTTGARTGAATGGGCA 0.107 0.1020.450 0.601 2.812 25 7 149768593 6946579 6734 TTGCCTAAGTRTTTAGAAAGT0.817 0.783 0.387 0.984 2.135 25 7 149784648 10227548 6735AAGAATCAAASCCCAGTGAAT 0.261 0.250 0.663 0.998 2.209 25 7 1497955072373845 6736 TATAGGAAATRTTGGCAATTT 0.089 0.089 1.183 0.903 0.894 25 7149801383 16884586 6737 CTCAGCAACAYGGGAAGACAT 1.708 1.646 1.097 0.7740.955 25 7 149825654 17173566 6738 TTGTACAGTCYAAGTTCCTTC 1.215 1.1541.025 1.114 0.957 25 7 149852220 6464094 6739 GTTAGGAATAYAGGATTCTAT0.690 0.657 1.476 1.098 1.065 25 7 149868951 17173583 6740TTTACCTGCTYTTTGATTTTC 0.048 0.040 0.941 1.147 0.765 25 7 149878297 65986741 TTAGCCAATARATTTCCTACT 0.686 0.671 0.690 1.386 0.771 25 7 1498942464725360 6742 AAGAAAAATTRAAAGAACCAA 0.911 0.840 0.533 0.855 0.624 25 7149905239 17255187 6743 ACATAGTACAKAATGAATCCT 0.741 0.713 0.747 0.4530.618 25 7 149914536 7806458 6744 GTATTAAGACRTAAATGGGAT 0.299 0.3030.673 0.285 0.681 25 7 149921160 12669824 6745 AGGAATTAATRCTATCTTCAG0.500 0.461 0.312 0.351 0.406 25 7 149928024 2072443 6746GCAAAGCCTGYCAGGGTGAGC 0.000 0.000 0.077 0.241 0.346 25 7 1499402567785283 6747 TCCTAAAACTSCTTGATAAAA 0.081 0.083 0.075 0.112 0.234 25 7149948563 2888674 6748 TATGGCCCCARTTGGCAAAGC 0.050 0.048 0.034 0.1010.228 25 7 149955253 9640170 6749 ATTTATCTGASTGAAGAATGG 0.240 0.2370.033 0.220 0.213 26 7 150075668 2968855 6750 TCAGAGGCATYCCAGAGCATG0.183 0.179 0.023 0.049 0.224 26 7 150104858 3807375 6751AAAAGGAGCAYTCTAAACGCA 0.155 0.158 0.035 0.004 0.303 26 7 1501075153778873 6752 ACCTGCAAGTSGAATGGGTTG 0.105 0.111 0.051 0.121 0.238 26 7150118625 2373962 6753 AATGCCCTGASAGTTTGCAGA 0.389 0.384 0.042 0.2500.165 26 7 150140429 3918188 6754 CGTACAAGGGMGTTTGAGAGA 0.184 0.1800.357 0.355 0.118 26 7 150150382 3918216 6755 TATGGGGCCTSTAACAATGAC0.123 0.122 0.663 0.348 0.601 26 7 150166860 6947821 6756TGGAGATACCYGCACTTGCTT 1.320 1.304 0.696 0.336 0.598 26 7 1501814684148854 6757 GGGGTGTGATRCAGTGCATTG 0.902 0.891 0.682 0.463 0.813 26 7150195045 6464120 6758 TCCAGGCGGGRAGTTCTGTGC 0.472 0.449 0.672 1.1121.020 26 7 150206434 2303937 6759 GAGAGAAGACRCAGGACCTGA 0.134 0.1340.383 1.147 1.172 26 7 150212992 2303943 6760 ACGAGCTGCAMGTGACTAATC0.086 0.085 1.141 1.430 2.368 26 7 150234900 4236428 6761TGGGGTGTCCYGAGCCCAACT 0.628 0.631 1.041 1.474 2.778 26 7 1502468126951528 6762 CCTGGGCTGAMCCAATGAGCA 2.586 2.450 1.491 1.460 3.026 26 7150260307 6464131 6763 GGTGCCCGCTSCTTCCCGTTC 0.301 0.294 1.945 3.3173.434 26 7 150269456 6979622 6764 TGTGCCCTAAYGCAGCCCTCG 1.000 0.9492.042 3.526 3.250 26 7 150286812 6464132 6765 GCTTCGTGAARGTAGGTATTC0.982 0.926 2.721 3.467 3.031 26 7 150295919 4725395 6766CTTCTTTCCARTTAAAATATT 0.752 0.759 2.681 4.036 2.701 26 7 1503126604726005 6767 ACACAGAAGTYATCACTCACA 4.390 3.835 2.450 2.486 3.217 26 7150320869 2257069 6768 GCAATTTCCCRATGCGGGCCA 1.294 1.226 3.665 2.5843.364 26 7 150348102 2608288 6769 AGTAAAGGGASAAAGTTCTTC 0.174 0.1603.375 2.221 4.225 26 7 150355235 7784344 6770 GGCTATATTAWAAACTAATAC0.953 0.947 0.584 3.153 2.533 26 7 150362605 3807386 6771CCCGCTGCCGMATTGTTAGGT 0.097 0.087 0.154 3.375 3.245 26 7 1503737001870238 6772 AATGACCACASGGCTGCTGTC 0.308 0.306 0.480 0.711 2.743 26 7150402025 7799570 6773 GACTATTACTRTATCCATTTT 0.030 0.030 0.366 0.3602.838 26 7 150429874 219254 6774 TAAATCTGTGRCTGAAATCTT 1.154 1.116 0.5351.405 2.641 26 7 150432996 219271 6775 CCATCATGGGYGTGGCCACAG 0.643 0.7150.507 0.943 0.835 26 7 150444177 6964641 6776 TGGAAACCTCRAAGCTACATC0.436 0.421 1.958 1.009 0.510 26 7 150454523 17705372 6777TTTCAAATACRTCTCTGACCC 0.243 0.237 1.272 0.924 0.606 26 7 150457561760234 6778 CCTCTGCTTTYTAAGGGACAT 2.577 2.567 0.988 0.985 0.356 26 7150478042 2063995 6779 GTCCTGATTARCCTAATTCTT 0.078 0.076 0.858 0.5890.375 26 7 150484480 414168 6780 GGACTGATCGRTGCCAGCACT 0.270 0.272 0.8060.497 0.337 26 7 150491281 11771465 6781 TACTAGAGAAMCTTGTCCCCT 0.0510.052 0.013 0.372 0.791 26 7 150504189 13234689 6782AAAATTGTTTYTTCATGAGGT 0.170 0.163 0.044 0.335 0.579 26 7 15053277912703130 6783 TATCTACACARAGCTATGGGT 0.175 0.176 0.020 0.003 0.418 26 7150540515 4726024 6784 TTCACAGGGARTCTCCTATGC 0.354 0.425 0.025 0.1320.428 26 7 150561177 17712606 6785 ACTGATAACCRGTCATTGCCA 0.071 0.0690.028 0.152 0.365 27 7 156602277 6976552 6786 TTAAATTTCTYCGCAGCTTCA0.599 0.547 0.913 0.846 0.725 27 7 156641263 4716470 6787TGTTGCTCACSCTTATCTGGA 0.452 0.452 0.548 0.582 0.565 27 7 1566649964716472 6788 TGAGGCTGAGRTGTGTCCCGT 0.249 0.256 0.171 0.433 0.566 27 7156681516 10255470 6789 ATCTCTGTTAYTGATTGGCAG 0.006 0.006 0.057 0.2960.603 27 7 156693363 10440911 6790 TCTTCAGTCGYGTACATGATT 0.044 0.0450.047 0.067 0.652 27 7 156700081 6952964 6791 CCTGGTCGGGYGCCCTGGCAT0.106 0.109 0.045 0.033 0.514 27 7 156705616 17837758 6792TCAACAGCTCKAAGTTTGTCT 0.490 0.451 0.041 0.051 0.363 27 7 1567096972072421 6793 TTACGATTACRAAGGATGAAA 0.358 0.360 0.068 0.196 0.197 27 7156728759 10242467 6794 ACAATACAGCMTGGAATTTGA 0.040 0.040 0.227 0.3000.242 27 7 156758455 10807645 6795 TGTTTTATGCYATATCATTTC 0.254 0.2390.580 0.356 0.233 27 7 156760145 2366564 6796 CTAATGAAGTRTGTAAACCTC0.749 0.724 0.647 0.473 0.865 27 7 156779766 221216 6797GATATCACGGYGGATGAGAGT 1.367 1.354 0.784 0.605 1.493 27 7 156789143758925 6798 TCCAAATCCTYGGCCCGACAT 0.000 0.000 1.017 0.662 1.553 27 7156795541 17667159 6799 GTTCCAGGGAMGTGCTGCTGA 0.235 0.237 1.013 1.7121.732 27 7 156808554 1468649 6800 TGGGCGGGTGMAGCAGCCACC 0.000 0.0000.486 2.286 1.728 27 7 156814282 10260168 6801 TCCCTGGCCTWTTGTAAATAA0.794 0.792 1.604 2.267 1.804 27 7 156821109 221232 6802CCACACAAGCYGCATGGCCAA 0.358 0.555 2.484 2.041 1.873 27 7 156834693221258 6803 GTGCTAATCCYGCAGAGCTGC 2.581 2.463 2.243 1.884 1.899 27 7156838212 221271 6804 TTCGCATGGTYCCATTTCCTT 2.101 2.287 2.353 2.0331.955 27 7 156862087 221295 6805 AAAGATGTGAMTACCCAGCAG 0.687 0.683 2.0541.815 1.561 27 7 156866999 221300 6806 TGTGAAGCTTYCTGAGAATGT 0.900 0.9101.387 1.791 1.465 27 7 156881375 2021743 6807 GGTGGAATGAYGGGAGGCTCA0.306 0.300 0.708 1.762 1.743 27 7 156889073 17837784 6808CAAATTTTAAYCCTATGCAAT 0.877 0.850 0.566 1.009 1.775 27 7 15689569317837785 6809 GGTCTCTTTCRTAAGAAACAG 0.287 0.291 0.441 0.417 1.741 27 7156912409 — 6810 ATTGGAACCTRCTATACACAT 0.319 0.295 0.328 0.541 1.746 277 156919171 1263563 6811 TAACAGCAGTYGTATAAATTT 0.638 0.636 0.097 0.4151.086 27 7 156927403 17837786 6812 GCCTGGTACAMCCTACATGAT 0.020 0.0210.371 0.454 0.654 27 7 156934044 17837788 6813 AGCTACCTGAYGTCTGGCTCA0.174 0.125 0.466 0.426 0.589 27 7 156952480 1638027 6814TGTGATTTGCYACCCATGGGA 1.120 1.131 0.359 0.462 0.817 27 7 15696449017731604 6815 ACGTTTGGGGMTCACTTAACA 0.530 0.499 0.734 0.520 1.240 27 7156969688 17837795 6816 GTACACTGCTYTTGGAAACTG 0.395 0.396 0.863 0.4721.047 27 7 156992288 7811860 6817 AAGAAAACCARAAATCTAGTC 0.783 0.7840.535 1.204 1.116 27 7 157005136 7802459 6818 TAAACGCCTGYTGTTTGAAGG0.388 0.374 0.501 1.802 1.095 27 7 157013152 7792857 6819TGGGGAGGTARGTGTGGGGGC 0.000 0.000 1.144 1.365 1.156 27 7 1570437141796286 6820 TCTCTGTCCTYGGCGTGGGAG 0.471 0.466 1.951 1.345 1.212 27 7157070372 6949185 6821 CCAACGCTCCRCCTGCATGGC 1.724 1.703 1.666 1.3001.155 27 7 157082642 4716494 6822 GAAGATCAGCRTTCAGACCAG 1.474 1.6321.666 1.363 0.963 27 7 157093892 7783909 6823 CAGGAGAGGCRGCTGCTGAAT0.210 0.207 1.568 1.270 1.781 27 7 157104944 870020 6824CCCACCACAGRTGGGTAGCTG 0.000 0.000 0.810 1.012 1.737 27 7 1571343987777198 6825 CCAGGCCTCCYCCACACATTC 0.249 0.241 0.187 1.036 1.922 27 7157145415 1560962 6826 AGACCCAGAGRCTCACGCTCA 0.691 0.659 0.144 1.5781.771 27 7 157158172 7788108 6827 GCTGCCGCGCMACCTCTGACA 0.211 0.2070.144 0.818 1.621 27 7 157182316 731305 6828 ATACATTTCTYGGAGTTTGAT 0.0430.044 1.440 1.171 1.400 27 7 157198659 4716509 6829GTGAGGCCCAYGAGGGGCCCC 0.000 0.000 1.135 1.002 1.520 27 7 1572135556459817 6830 CTCTGTTTGAYGCTATGATGA 2.727 2.620 1.684 1.025 1.458 27 7157226309 1991963 6831 CCATCTTGCCRCTGGTGACGT 0.175 0.179 1.693 0.8641.627 27 7 157237334 6977123 6832 CGGAGGCCCCRGTGCTGGGTG 1.121 1.0761.484 1.729 1.724 27 7 157250123 6955761 6833 CTAAGAACCARTATCCCTTAT0.261 0.274 0.280 2.515 1.736 27 7 157265078 4716857 6834ACTCCACACCRTGAGAACAGG 0.089 0.091 1.099 2.515 1.725 27 7 1572777371036373 6835 GATGAAAATGYAGTTATACCA 0.257 0.258 1.330 1.434 1.944 27 7157280884 1347391 6836 AGGTCATGCAYGTCCACACTT 2.016 2.029 1.599 1.3642.384 27 7 157328316 6951695 6837 AATTAGGGGCRTAACAATAAC 1.300 1.3171.823 1.105 2.384 27 7 157342999 3935208 6838 CTCTTTATGCMAAGAGGCACT0.000 0.000 1.785 1.261 1.437 27 7 157353794 7455546 6839ACGGAGGAAGSCTGTGGCTCC 0.855 0.857 0.945 1.641 1.557 27 7 1573688601806465 6840 CCTCTCACCTKCAGCCCCTGA 0.142 0.134 0.586 1.798 1.402 27 7157379611 7780849 6841 AAGAGCAGGARGAGTTTCTGA 0.564 0.570 0.935 1.1921.304 27 7 157405658 2335844 6842 AGGAGTAGGASAATGGTAACA 0.636 0.6160.797 0.839 1.436 27 7 157418058 4909094 6843 GGCTGCACCCYGTCCTGGCAC1.172 1.253 0.936 0.889 1.738 27 7 157434414 4909107 6844ACTCCAGGCCRCTGCCCTAGT 0.000 0.000 0.946 0.648 1.040 27 7 1574693772335836 6845 TTGCATTCCCSCTAATTTGAG 0.355 0.373 1.004 0.819 0.694 27 7157493079 6963290 6846 AAATTATTTAYGTGATTTCAT 0.000 0.000 0.396 0.9980.610 27 7 157500728 6942903 6847 CAACAAAAATRCATTTCAAAT 0.727 0.7120.396 0.764 0.555 27 7 157525134 6950346 6848 CCACAGCTAAYACCATAATGA0.235 0.237 0.737 0.368 0.660 27 7 157536873 4909053 6849GTGTGTGTGGKTGTGAGTTGT 0.000 0.000 0.485 0.301 0.555 27 7 1575488972001763 6850 TTTGCCCGAGYTTAAAATTAA 0.992 0.969 0.281 0.397 0.518 27 7157564283 4909164 6851 GTGAAAATGAYCACAGGGTGC 0.067 0.068 0.288 0.3970.292 27 7 157577455 896769 6852 CTGTGCTATGWAGGGACAGGA 0.266 0.273 0.3930.250 0.508 27 7 157590205 6950401 6853 TAGTGTTTTCYTACGATTCTT 0.2040.210 0.199 0.313 0.429 27 7 157602000 7790889 6854CCCAAACGTAMGTGCTGGAAT 0.670 0.673 0.264 0.286 0.454 27 7 1576163384909064 6855 GTCTCCGCATRGACAAGACGG 0.000 0.000 0.296 0.388 0.374 27 7157619962 7782393 6856 TGTAAATCCCRGTGCCCTGGG 0.244 0.238 0.359 0.3980.600 27 7 157654735 6459868 6857 TGTCCCCTCTRCAAGGGACAA 0.000 0.0000.686 0.481 0.518 27 7 157667519 4909077 6858 AGGCACAATGWGGGCAGCAGA0.334 0.325 0.464 0.531 0.381 27 7 157681505 896762 6859AAGTGAAAGCRCACTTTGACT 1.344 1.323 0.645 0.690 0.829 27 7 1576938524909210 6860 TAGTAAAATAYCTTGATGATC 0.065 0.067 0.559 0.572 0.809 27 7157696513 4909212 6861 ATGCTGAGGAKACAGAGATTC 0.599 0.580 0.969 0.6281.044 27 7 157716083 4909085 6862 TTCTGCTGTGSTCTGTAGTTT 0.358 0.3220.410 1.124 1.555 27 7 157731429 1476199 6863 CACCTCCTGCRCATGCCTCAG1.173 1.143 0.530 1.064 2.450 27 7 157784389 7788773 6864GTCCCTGGTCMCGGCTCTGGC 0.184 0.179 1.153 0.937 2.535 27 7 1578887701882247 6865 GAGAAGCAACMTGTGATAATC 0.350 0.354 1.019 1.689 2.535 27 7157900851 6972776 6866 GGAATCCCCARTGCCAACACA 1.792 1.868 0.973 2.4342.397 27 7 157910068 7788516 6867 CTCACTAACTKGAGGAGATGG 0.220 0.2221.752 2.471 2.178 27 7 157927271 4909243 6868 AATCAATTTTYTGAGAAAAAC1.010 1.014 2.544 2.349 2.192 27 7 157939751 3793177 6869TTTCCACCTGYAATAGCTGAG 2.484 2.320 2.296 2.308 2.129 27 7 1579634602290391 6870 AAATACAATGSAAGGCAGGGG 3.835 3.488 2.698 2.295 2.359 27 7157979187 963177 6871 GAAGCCACCCYGGCCAGGCCA 0.000 0.000 2.816 1.9302.193 27 7 157991785 1037458 6872 CCAGGCCCACYGTGCTCTCCA 0.000 0.0002.243 2.290 2.240 27 7 158007683 7799553 6873 AATTATAGGCYGAGTGAGGTG0.009 0.009 0.377 2.713 2.177 27 7 158031369 7800556 6874TACACGATAARATAATTTCTA 0.352 0.325 0.377 2.531 1.906 27 7 1580445101189191 6875 AGCCATGAAGKTGTTGACTCA 0.922 0.910 1.325 1.205 2.096 27 7158055182 844521 6876 TTAAAACATAYTTTTGATTAT 0.000 0.000 1.710 1.0982.061 27 7 158071349 842444 6877 GATCAAAGGCRTCAAACATAG 2.545 2.455 1.5880.947 1.762 27 7 158084298 7780170 6878 CGGCTACTTTMCTGAATTTAT 0.7760.750 1.440 1.154 1.010 27 7 158097267 2279881 6879ACATCAGTGGSGATCTCTGCT 0.133 0.221 1.194 1.255 1.040 27 7 1581164691154002 6880 TTCAGGTGCTRAAGAAGCACT 0.422 0.420 0.280 1.068 0.950 28 926700092 10511781 6881 CACCAGTTCARTCACCAATGT 1.189 1.192 0.651 0.7371.002 28 9 26714384 7866709 6882 ACCTTCTGTAWGTTCAAGTTC 1.351 1.363 0.6100.386 1.098 28 9 26715878 7028218 6883 CAAGTGGGAAYGGCAGAACTG 0.039 0.0390.687 0.412 0.870 28 9 26731583 17693460 6884 ACCATGCCCCRGGTCCTGTGC0.120 0.118 0.337 0.350 0.635 28 9 26739795 10967513 6885ATCACTTAATMACATTCATGA 0.407 0.381 0.073 0.616 0.616 28 9 267492157871924 6886 AAATTTGCCTRATCTGGTCTC 0.054 0.053 0.056 0.310 0.567 28 926757842 12350711 6887 CTATTTTGGAYTGCTTTGATG 0.531 0.535 0.320 0.1710.550 28 9 26766151 17760488 6888 TTACCTACAAMCTTGAGGAAG 0.030 0.0290.227 0.480 0.656 28 9 26773300 17693816 6889 ATGGAAAACAYGGAAAGGAGA1.113 1.146 0.473 0.534 0.452 28 9 26779940 1889899 6890AAGAGTAGTTRGTTGACCAGT 0.044 0.045 0.883 0.456 0.317 28 9 2678616010116852 6891 TTGTTTGTTCRTTCATTTTCC 0.733 0.742 0.976 0.676 0.330 28 926791741 17694003 6892 GGGGTTATACKCTCTGCTTAG 1.369 1.374 0.526 0.5570.393 28 9 26798068 12000467 6893 TACAATACACSCTGACATTAT 0.226 0.2310.828 0.725 0.356 28 9 26816017 10967550 6894 GCTAGTTGTGRTATAGAAAAT0.143 0.146 0.560 0.446 0.391 28 9 26822939 17760657 6895CATGAAAGTCRGTCTCCTCAC 0.750 0.752 0.292 0.539 0.341 28 9 2682950117694087 6896 GTGTTCAACARAAAGATGTGT 0.206 0.197 0.255 0.405 0.347 28 926847244 1889901 6897 TGCACACATAYACCTACACAT 0.643 0.640 0.375 0.1430.269 28 9 26856642 7036871 6898 CTTTGAAGCTYGGGACCTTAA 0.115 0.116 0.1970.146 0.399 28 9 26868374 10511791 6899 TTGGCGCTCASGTTTAGTAGC 0.4100.416 0.163 0.149 0.293 28 9 26876393 7857990 6900 GAAGGGAATGYATAAACTTGT0.172 0.171 0.083 0.131 0.118 28 9 26883662 7038314 6901CAGGAGTTCCRTTAAGTATCA 0.221 0.222 0.066 0.230 0.175 28 9 268891307860542 6902 CTCAACACCTRATACATTACC 0.264 0.262 0.089 0.118 0.267 28 926894699 16910873 6903 TACAGTTTGAKGTACTCTTGC 0.020 0.020 0.278 0.0970.193 28 9 26900951 10967594 6904 TTATTCCAAAKGATGTAGTAG 0.571 0.5620.232 0.142 0.176 28 9 26909666 16910888 6905 TAAAATCTTCSTTTTTGGCAG0.852 0.826 0.182 0.265 0.395 28 9 26914621 10511793 6906CTTTCCATTCYTTGACTCCTC 0.009 0.010 0.392 0.269 0.406 28 9 269259967045881 6907 CAACCAGCAGWTTATGATTTA 0.055 0.053 0.450 0.255 0.342 28 926938134 10511794 6908 CCCCTGGTTTSGGAGATCATT 0.655 0.665 0.204 0.6941.014 28 9 26946868 7857396 6909 CAGTTAGCAASTTTCCACTTA 0.717 0.775 0.2510.600 0.952 28 9 26952769 12352441 6910 GACAGAAATARCAAGTACTGA 0.0940.090 0.922 0.437 0.973 28 9 26980826 17694499 6911TGAGTTAACTWGGGATACTCA 0.104 0.101 0.769 1.478 1.269 28 9 2699433810967655 6912 TCAGCTCAAGRTAGATTCTGT 2.095 2.085 0.530 1.439 1.235 28 926999738 17756299 6913 CTGCTTAATAMACAGCTAACC 0.212 0.202 2.534 1.4851.221 28 9 27008020 12337896 6914 AATCATTTCARAAAGGTATTA 0.055 0.0572.842 1.735 1.216 28 9 27018947 7390085 6915 TGGAAAAGATRTCTAACCTCC 4.3404.062 1.692 2.035 1.341 28 9 27031315 7045747 6916 ACATTCCTGTYAGAGCACTTA0.052 0.052 2.756 2.499 1.324 28 9 27037460 16910953 6917GGCTCACTATYGTATCTTCTC 0.171 0.180 2.848 1.503 1.194 28 9 270405931413824 6918 CCTGGAATTAMTACAGTTAGT 1.388 1.387 0.358 1.719 1.288 28 927057529 10511798 6919 AGAAAGAACTRTTATTAAATT 0.063 0.062 0.393 1.8041.318 28 9 27071879 16910976 6920 TTATAATGCTMATCCAAGCCA 0.437 0.4660.540 0.216 1.016 28 9 27088940 1161603 6921 AAGGTGAATARAAATGTAACT 0.1480.132 0.132 0.210 0.991 28 9 27096614 10967715 6922TTGGGGGCACRTATTTGATTT 0.549 0.538 0.191 0.196 1.103 28 9 27104804 6807396923 AACCAGGGCCWTACTTAAGAT 0.253 0.251 0.081 0.120 0.117 28 9 2711505612339737 6924 CCCAACCAGCRTGGGCTTGCA 0.247 0.281 0.090 0.127 0.263 28 927122514 7044842 6925 ACAGTGTCTTYCCTGGATATT 0.005 0.006 0.203 0.1100.278 28 9 27129817 10967731 6926 TAGTACAGCARGAGTAACAAC 0.150 0.1540.146 0.112 0.129 28 9 27143055 7855848 6927 AGGATTGTTAWGCTTCACTTG 1.0000.989 0.155 0.204 0.234 28 9 27153395 506685 6928 CTATGAGGGCRTCTAACCTGT0.059 0.060 0.201 0.213 0.303 28 9 27159124 3818283 6929GCGATTATAGRTATCTCCTGG 0.351 0.337 0.562 0.244 0.694 28 9 2716622911791294 6930 TATGTATCAGKGCTGGTTTCT 0.162 0.166 0.276 0.443 0.650 28 927175762 17834811 6931 CTTTAAAAAAKTATATGCATG 1.062 1.090 0.412 0.6490.600 28 9 27220196 1407300 6932 CTCATAGCTAYTGCCACATGT 0.273 0.268 0.5800.993 1.129 28 9 27229343 17695236 6933 CTGTGGAAATRTAACCTTTAA 0.4440.430 0.876 1.130 1.296 28 9 27240868 4879313 6934 ACTTGGCTTAKTTAGGGAATA0.763 0.743 1.324 0.967 1.297 28 9 27249071 17695332 6935TGGCTTCCTTRCTTTATCCTG 0.827 0.838 1.429 1.884 1.677 28 9 2725782510812556 6936 GAGGGATGAARAAAATCTTAA 1.883 1.978 1.186 1.635 1.793 28 927264428 13288748 6937 CGCTGATTGTRATCCAATGCA 0.383 0.377 2.188 1.6621.610 28 9 27273000 10967855 6938 ATTTATCTGAYTTACCTGAAC 0.056 0.0571.895 2.387 1.795 28 9 27283009 7873922 6939 TGGGTCCCTCYAGAGGAATGT 1.9091.914 0.740 2.210 1.843 28 9 27289620 17778819 6940ACAAATTAAGYCCAACTTGTG 0.431 0.414 1.575 1.705 1.705 28 9 2729716310967874 6941 ACTGGCTGAGYGGAACCTGTC 0.241 0.244 1.568 0.936 1.671 29 970132969 2182739 6942 TTCTTAATTCRTTTTTCTGCT 0.108 0.102 0.530 0.1780.333 29 9 70141432 7038061 6943 TAAAATCTCASAATATTAGTC 0.188 0.183 0.2850.204 0.428 29 9 70150928 17052748 6944 AAATTATACARTGGACTCCAA 0.6040.595 0.055 0.343 0.420 29 9 70163512 7025228 6945 AATAACAACASCATTTCCACA0.135 0.137 0.060 0.467 0.620 29 9 70168957 7851212 6946TCCAAAATCTYTTCCCAAAAA 0.075 0.075 0.252 0.483 0.716 29 9 701861124610818 6947 TTAGTTTATTKGCTATCAAAC 0.112 0.115 0.510 0.552 0.714 29 970192722 4745004 6948 TAATACCTTGWAACTCTTTTT 0.920 0.899 0.860 0.8600.909 29 9 70200766 17053503 6949 CAGTGCAGGAWAGATTTGGGA 1.278 1.2510.947 0.832 0.734 29 9 70207772 10491653 6950 TGCAGAAATAYTGTAATTAAT0.977 0.961 1.543 0.837 0.940 29 9 70215494 17053650 6951TTAGATACCGYGGCCTAAAAA 0.292 0.302 1.449 1.390 0.900 29 9 702193864745008 6952 GAATCCGGCTRCTTTAGGAAA 1.226 1.223 0.832 1.426 0.913 29 970231043 10868816 6953 GTGGGAGTTTYGTAGGAACAC 0.492 0.465 1.112 1.3661.032 29 9 70243909 11142398 6954 CTTTGTTCAGMTATTTGTGGG 0.184 0.1820.995 1.010 1.064 29 9 70284062 17455513 6955 CTGTTCACCTRAAGTTACTTT1.568 1.612 0.869 0.848 1.062 29 9 70295308 2184756 6956TTTTATCCTTYCAGTGTTTCA 0.107 0.108 0.707 0.960 0.843 29 9 7030017213299487 6957 TTTGTAGCTTWTACAGCTGTG 1.030 1.010 0.960 0.672 0.902 29 970317582 7864965 6958 TTGCTTGGACRCCTCTCTTCC 0.058 0.059 0.509 0.5281.310 29 9 70326189 6560136 6959 AGTGGATTCCYTTCTTTGGAG 0.653 0.690 0.5390.475 1.389 29 9 70333865 10868838 6960 TTTATGGCAGWCTCAAAGGCA 0.5900.632 0.152 0.440 1.458 29 9 70339788 950840 6961 GAGAGCATGAYGTAATCTTTC0.161 0.172 0.141 1.349 1.433 29 9 70345328 7856388 6962TCTGGAGAAGKTTCAAAGATG 0.008 0.008 0.426 1.228 1.962 29 9 7035423617535530 6963 AAAGGGTACCRAGAAATAAGT 0.040 0.041 1.548 1.885 1.845 29 970360083 17535600 6964 TCTATGTACARGTGCCAGAAA 1.472 1.449 1.964 1.6011.962 29 9 70367841 7046672 6965 CTACTTTTCCYGAAATCTCTT 2.612 2.942 2.9301.841 1.734 29 9 70376992 17535865 6966 TCATGAAAATYGTACCCAAGC 0.0000.000 2.663 2.590 1.789 29 9 70385945 1932701 6967 AAGGTTACCTRTGTTTGTTTC1.111 1.124 2.432 2.686 1.485 29 9 70395370 7034027 6968TATCACAGGGRACACTTGAGA 0.371 0.355 1.654 2.703 1.265 29 9 7040196617536713 6969 AACCTAAAAASTGCTATGACT 1.179 1.198 1.489 1.986 1.196 29 970409531 2871324 6970 AGAATCCAGAYAATAGGCAGT 1.480 1.618 0.990 0.9221.314 29 9 70416448 — 6971 CATCTTGACTKTTGGCATTTG 0.321 0.318 0.971 0.7691.478 29 9 70422125 12686512 6972 TGCTTAAGTAYGACTGCCATA 0.291 0.2890.602 0.507 2.228 29 9 70433166 10735599 6973 GAGGCAAGACRTCGTAGGGCA0.331 0.309 0.127 0.551 1.575 29 9 70443198 4744606 6974TTTTCAGCCCMGTTGGGAAGT 0.311 0.301 0.077 0.403 1.763 29 9 704502877865398 6975 ATGAATATTGWTAACTGCCCT 0.104 0.103 0.118 1.249 1.518 29 970461216 11142508 6976 AGAGAGTCCCYTTACACACAA 0.045 0.046 0.187 1.2351.691 29 9 70477224 7045775 6977 TTCCTTCTTCYTCCCAAATTC 0.530 0.535 2.0591.586 1.638 29 9 70484986 1538669 6978 AATACCCTTGSCCAACCAAAG 0.653 0.6252.452 1.675 1.338 29 9 70497790 11142532 6979 ATGGAGAGAAYAGAGGAATGT4.337 4.091 3.308 1.912 1.271 29 9 70509554 11142536 6980TGGCTGACAGYGCCTCAGAAA 0.104 0.106 3.004 2.084 1.228 29 9 7051518211142540 6981 GGAATTTTTARAACCCGTTTC 1.413 1.413 2.995 2.113 1.236 29 970525093 1856655 6982 CAACCATGACKCTACAGCAGG 0.157 0.158 1.095 2.0081.524 29 9 70541921 4745026 6983 ACATAGAAACRTACATACATT 1.483 1.510 1.1961.845 1.652 29 9 70543565 1160742 6984 TAAATGACAARTAGTCTATGT 0.699 0.6920.676 0.579 1.932 29 9 70591948 10511986 6985 TGGACTGAAAYTGCAGGCAAA0.486 0.520 0.673 1.020 2.040 29 9 70599241 7849151 6986CAAAAGAAACYTGAAGGATAC 0.082 0.082 0.155 0.728 1.951 29 9 7060661111142569 6987 TTGCAGCGTCMTGTCTAATTG 0.161 0.163 0.518 0.979 1.068 29 970612919 17055769 6988 GTGTGTATGCYTGGGTCAGCC 0.094 0.091 0.442 0.6871.058 29 9 70619595 1831144 6989 TTAATCCACTRTATCCCTAGC 1.686 1.706 0.7550.586 0.740 29 9 70626414 7847799 6990 ACCTGAGAAASCTGAAATGCT 0.335 0.3301.018 0.781 0.888 29 9 70632742 11142592 6991 ACCTTAGCTGWTCATAAGCCT0.773 0.761 1.143 0.750 0.689 29 9 70638656 1328158 6992GGGCTTCCTTKCCAGCATAAC 0.626 0.624 0.806 0.744 0.530 29 9 7064452210780969 6993 GCAGCATTGARCAAAGTTGAG 0.376 0.374 0.665 0.948 0.560 29 970650613 17463001 6994 CTTCTGCCTGSGATTTAGGAC 1.049 1.049 0.409 0.5930.563 29 9 70657059 12552572 6995 GGCCATTCTASCACTGTGTAT 0.057 0.0520.372 0.510 0.562 29 9 70664548 560819 6996 AAAAATCCTARTGCCATTTAT 0.0000.000 0.541 0.468 0.619 29 9 70672189 1034539 6997 GCTCTGGCCCRGTAGAAATAA0.536 0.556 0.191 0.324 0.259 29 9 70674554 10868901 6998GGGTAAGACCMAAGCAGTCTG 0.818 0.822 0.370 0.279 0.206 29 9 706834591932926 6999 CTGTTTTACCKCCACACCCAA 0.075 0.076 0.378 0.132 0.233 29 970697458 10868911 7000 TTTTCCCATGKGTCAATTTTA 0.619 0.612 0.245 0.1270.210 29 9 70703724 10780979 7001 AGACAACTCTRAGTTCCACAG 0.137 0.1370.106 0.100 0.188 29 9 70710180 1337024 7002 TTAGGCAAGCRTGTGGCACCG 0.1780.167 0.095 0.160 0.200 29 9 70722520 1609625 7003 TACTGACTTGRCTTGAGGCCA0.340 0.333 0.010 0.109 0.216 29 9 70730035 11142636 7004TTTTTACCCARCTAGGTCATT 0.022 0.022 0.121 0.134 0.330 29 9 7073665010511992 7005 TTTTGGAAGAYTATATAGAGA 0.013 0.013 0.214 0.236 0.349 29 970746659 4143736 7006 ATCCTTGCAARCAAATGACAT 0.910 0.904 0.201 0.2400.267 29 9 70757996 11142642 7007 TCAAAACTACSAGAGGGAAAA 0.473 0.4550.620 0.385 0.265 29 9 70765970 3010419 7008 TAGTCCAGGARACAGAATATT 0.2570.253 0.692 0.473 0.207 29 9 70775011 2993024 7009 TAAGTCACAGMATCTTAGTAT1.113 1.139 0.579 0.553 0.253 29 9 70782222 17056028 7010CTCCTTTTTCWAACAAAGCTC 0.161 0.158 0.669 0.573 0.344 29 9 7078818612351255 7011 TCAAACTCTCYGATGCTGAAT 0.736 0.705 0.699 0.387 0.358 29 970794271 3010423 7012 TGAGTTAGTAMAAACTTGGTG 0.641 0.653 0.286 0.3920.364 29 9 70800561 7022875 7013 GGGTGCCCGAYTGAGAATCAC 0.326 0.323 0.3160.529 0.416 29 9 70808080 1337022 7014 TGCGCTTTTTSCAAGTGTGCT 0.096 0.0990.233 0.327 0.376 29 9 70814967 — 7015 ATGGGCCTTCYGTGGGGAGTA 0.253 0.2430.269 0.301 0.378 29 9 70821303 2909300 7016 TAGTGACTGCYTCAAAAGTGG 0.4480.442 0.291 0.215 0.494 29 9 70827862 1558924 7017 AAGTGCCTACYAGATATACAG0.773 0.724 0.290 0.226 0.520 29 9 70839607 17056164 7018TCAGCATTTTMTTGCCTTACA 0.378 0.384 0.311 0.255 0.673 29 9 7084682510868940 7019 TGGAGCCAGCRGTTTTAGCTT 0.037 0.037 0.377 0.448 0.711 29 970874370 11142672 7020 TGGACTGCTCRTATTTGAGTC 0.297 0.306 0.296 0.7150.599 29 9 70882519 573250 7021 ATTTTAATGAWCCTACTGAAT 0.677 0.700 0.4830.840 0.678 29 9 70890955 625699 7022 GCCCATAAAGYATCAAGGGAA 0.481 0.4880.920 0.848 0.677 29 9 70911110 659797 7023 ATAATTAACARCTCTGAGTTT 0.9340.894 1.041 0.768 0.691 29 9 70919059 661604 7024 TTAACACCCTMCTGCTTCAGG1.266 1.262 1.055 0.911 0.785 29 9 70925435 10868946 7025TTCTGGAATCMACTGTGTACC 0.926 0.970 0.983 0.897 0.682 29 9 70931529 5056767026 AAAATTTTTGRACACCATTGC 0.923 0.919 0.982 0.828 0.621 29 9 70932502656875 7027 ATGAGTCCCCRTTGTGCTTGA 0.046 0.047 0.623 1.004 0.637 29 970948361 1329748 7028 GAAATAATGGRGCAGATGATC 0.689 0.665 0.362 0.8190.609 29 9 70953863 10868950 7029 CTGAGTCTAGYTTATTACAAA 0.216 0.2170.371 0.474 0.521 29 9 70960548 17521958 7030 AAATTCTGCCYAACAATACAT0.315 0.308 0.449 0.288 0.535 30 9 93175798 2927567 7031ATGGAAACTAMCAACTACGTC 0.775 0.750 1.705 2.027 1.799 30 9 931819862995992 7032 ACCAGCATGTKTGTAGTCACA 0.272 0.276 1.938 2.108 1.961 30 993206088 4744229 7033 ATCGGGTGGCMCTATGACTGC 1.418 1.377 1.690 1.7691.691 30 9 93215235 11789033 7034 AAGGACTTTAYGCATTTGCAC 1.244 1.2181.573 1.684 1.706 30 9 93221289 7021710 7035 TGGAAGCACTYCACCTCTCAG 0.9760.930 1.690 1.501 1.677 30 9 93242815 7020852 7036 GGTCCTACACYCCAAAAAGTT0.855 0.868 1.279 1.218 1.409 30 9 93245937 4744239 7037GGAACGTCCAYGAATGGAGAG 0.250 0.262 1.039 1.449 1.324 30 9 932686647859267 7038 TTGTCCTCTGKTTTCCTATTA 0.559 0.573 0.511 1.097 1.286 30 993295671 1331588 7039 GAGCGTAAAARTGTTCATAGT 0.854 0.847 0.598 0.6431.051 30 9 93308995 10512224 7040 CAAATGCTGAKTCATCTTGTT 0.000 0.0010.629 0.541 0.991 30 9 93322209 4744245 7041 TGGGATTCCTYGCTGCAGTCC 1.0241.063 0.389 0.643 0.772 30 9 93324842 11791374 7042GCATTTACCAYGTAACCTTGC 0.361 0.362 0.326 0.582 0.614 30 9 933361381556416 7043 GACATTTGAARGATGCACCTA 0.012 0.011 0.788 0.501 0.457 30 993345702 16909211 7044 TTTAAAGTAARGAGCTCGTTG 0.642 0.644 0.439 0.4060.352 30 9 93359002 4743928 7045 GACCCAGGGCMGAGCTTACTG 1.211 1.176 0.3930.495 0.527 30 9 93368824 10739950 7046 ATCACCTACCYTTTTGAAGCA 0.1110.107 0.524 0.311 0.586 30 9 93377480 10821152 7047TATAGGGAGCYGATAAAATTG 0.242 0.235 0.432 0.253 0.514 30 9 9338629210821158 7048 ATACTGTTGAYGTTTTGAAGT 0.420 0.395 0.138 0.541 1.219 30 993388594 928367 7049 CCATGCTCCARTAACCTTCTC 0.431 0.421 0.136 0.596 1.58130 9 93422615 10821163 7050 TATTCTGTACSGAGTTCAGAG 0.097 0.098 0.4700.423 1.792 30 9 93427445 1000852 7051 CAAACATGAGRATCTGATTGG 0.186 0.1910.654 1.597 2.248 30 9 93437856 10821168 7052 GGCTTACCCAYGGTCTGTCCT1.322 1.244 0.661 2.827 2.304 30 9 93446202 2989751 7053GACGGAGAGCRAGACTTCTTT 0.893 0.878 2.824 3.149 2.088 30 9 9345935212551314 7054 TCCCAAGAGGMAATTGTGGGG 0.418 0.431 3.676 2.959 2.513 30 993475886 1806458 7055 GGTGTTCCTCRCAGGTGGTGA 4.364 4.488 3.723 3.0762.853 30 9 93485419 6479499 7056 AGCCCTCTAGWTCCTTGAAGT 3.119 3.149 3.3802.972 2.688 30 9 93491981 — 7057 ACCAACACGGYACACTCACAC 0.000 0.000 4.5683.198 2.674 30 9 93498640 10992831 7058 CTTCCGATTGRACTGTCCCTT 1.2791.214 2.171 3.428 2.942 30 9 93527276 7024542 7059 TACCTTCCAGYGCCATCAGGA0.921 0.880 1.487 3.454 2.926 30 9 93550895 7874616 7060CAAATGGGGAYTGTGGAAGAG 1.056 0.999 1.529 2.252 2.885 30 9 935637752398871 7061 CTACACGAGAYAACTGACATA 1.156 1.158 1.032 1.524 3.117 30 993569564 16909421 7062 AGGATCTGACMTTTTGAGTCA 0.642 0.636 1.145 1.4263.163 30 9 93590628 4392963 7063 AGCAGCTCTTMCCCTGTGGAT 0.003 0.003 1.0291.017 1.937 30 9 93619309 2263356 7064 GAAAATCACAYGTGTTGTAAA 0.973 1.0020.539 1.100 1.239 30 9 93634162 7030496 7065 TTTTTGATTGYTGATTAAATC 0.7590.754 0.293 0.683 1.060 30 9 93657887 12003661 7066AGGGGGATTASTTTGCCAAGG 0.280 0.238 0.788 0.335 0.719 30 9 936676883957506 7067 GGGACCGACCYGAGGATGAAG 0.066 0.067 0.379 0.376 0.887 30 993699974 3209003 7068 CCATACCTTGKGTGTTTGTAT 1.035 1.042 0.161 0.3980.949 30 9 93706342 12337678 7069 CTGGACCTGCYTGTGGTTCTT 0.059 0.0600.316 0.200 1.014 30 9 93714065 16909534 7070 ATGGGGGTAGWTTGTAATGAG0.109 0.114 0.293 0.352 0.847 30 9 93729092 10761299 7071TGTGTGGAGGYCACTGCACTA 0.782 0.774 0.065 0.672 1.303 30 9 9374709417625535 7072 CAAAAACATCWACGGAAAGGG 0.009 0.009 0.494 1.249 1.067 30 993756862 10733751 7073 CAGAACAAGGSCCACTGCAAG 0.208 0.207 1.056 0.8291.025 30 9 93764675 10992984 7074 AGCCTAGAATRTGACCTCTTG 1.383 1.3641.347 1.427 1.154 31 9 110265356 7031072 7075 GAGTGCTGACRGAAGTTAATG1.210 1.251 1.278 0.778 0.577 31 9 110272750 4978426 7076ATCTACAGATKCAGGGTCGGT 0.668 0.649 0.978 1.096 0.571 31 9 11028398716915037 7077 TCCTTGGGTAMAGGCTTTCAT 0.575 0.548 0.763 1.268 0.589 31 9110293281 4421419 7078 AACTTGACAGRACTTTAAGAC 0.298 0.303 0.631 0.9780.621 31 9 110299334 7875113 7079 TTGCTGCCTGMATAACACTGG 0.386 0.3820.681 0.784 0.614 31 9 110314062 10817025 7080 CCGTGAGGACYTGGCATTGCC0.900 0.956 0.465 0.405 0.700 31 9 110323375 3818766 7081CCTGTCTATCRCTATTTCTAG 0.704 0.726 0.469 0.288 0.645 31 9 1103501564427239 7082 CGTGCTTCCTRTTAGTCTCCA 0.104 0.106 0.379 0.169 0.517 31 9110358254 17734141 7083 TGAATGAAGGRAACACTTAAA 0.302 0.303 0.158 0.2490.315 31 9 110364426 2184245 7084 GCTGAATGTCYGCCTGTCCTA 0.152 0.1560.025 0.349 0.211 31 9 110375601 4300064 7085 ACATTGGCCAYCATCTAAACT0.200 0.205 0.124 0.227 0.247 31 9 110382596 10817036 7086CCTCTAACAAWGACTGGTATT 0.049 0.048 0.262 0.129 0.215 31 9 11038909210817039 7087 AGACTGTGGTRTGGGTAAAAG 0.646 0.661 0.321 0.110 0.220 31 9110399504 16915129 7088 TATTCAGTCAYAGTGTTTCCA 0.730 0.741 0.301 0.2040.113 31 9 110425355 16915134 7089 TCTTTCTGCCRTGAATTTAAC 0.378 0.3750.306 0.182 0.059 31 9 110444019 12236915 7090 CCTAGGGACCRGTTCCTGAAT0.135 0.137 0.360 0.213 0.130 31 9 110451178 4295728 7091GTTGTGCAATYTTTTTAACGT 0.086 0.084 0.145 0.226 0.155 31 9 11045754110980489 7092 CAGGTTTTTGYTCCCTTTTCT 0.789 0.796 0.131 0.133 0.307 31 9110463553 10980495 7093 TCCAGCAATTYCTACTCTAAC 0.041 0.041 0.117 0.1530.423 31 9 110474247 10521102 7094 GTATTTCTGCRTAGGTAGAAT 0.342 0.3420.140 0.165 0.598 31 9 110483333 3001114 7095 GATCCACTCAYGGGTTACACA0.115 0.120 0.143 0.394 0.735 31 9 110491782 3001153 7096TACATGTCTCMTCTGCCCTGT 0.136 0.140 0.297 0.608 0.791 31 9 1105016983010804 7097 ACAGGCTATGMAGTGTGGAGT 0.799 0.806 0.620 0.676 0.805 31 9110511161 3001125 7098 TGGATGATGAMAACCTTTTCG 0.454 0.441 0.909 1.1051.120 31 9 110541167 16915263 7099 AGATGATTTTYGCACACATTT 1.254 1.2171.414 1.323 1.201 31 9 110548452 4401940 7100 GGAATTTTCARTGAGGGTTTG0.887 0.924 1.968 1.446 1.083 31 9 110557083 10759461 7101CCCAGTTATGMGTTAGCTGCT 0.973 0.973 2.063 2.150 2.504 31 9 11057033110817087 7102 TGTCATTTAAYAGTTTGCTTT 1.284 1.292 1.445 1.778 2.681 31 9110586553 4144418 7103 ACAGCTAACTYAGAAAGACAG 1.085 1.050 1.643 1.7552.857 31 9 110597275 — 7104 CAATTTAAACYTGACAGGTAC 0.391 0.386 1.4033.271 2.824 31 9 110603895 1013820 7105 GGCCTCCTAGMAGAAGCGGAG 1.1871.160 0.816 2.695 2.375 31 9 110612005 10980564 7106TACGAATTTCRCAGGTTTTCA 0.357 0.362 2.903 3.206 2.612 31 9 110625482 —7107 TTTTAATTCTRTCATTTGTGT 0.223 0.219 2.857 2.252 2.157 31 9 1106486511409686 7108 TGCTCTGGTTMGTATTGGTAG 4.295 4.153 3.060 1.934 2.134 31 9110654946 1180281 7109 ATGGCATCATRCTAACAGACA 0.415 0.408 3.054 2.0251.987 31 9 110663567 2767001 7110 CAGTGAGAGGRATCAAATGAG 0.446 0.4052.496 1.600 1.649 31 9 110673638 12555499 7111 TGGATTGCCTRTGATACTTCT0.056 0.043 0.161 2.049 1.402 31 9 110691415 526584 7112AAACTGAATGYTCATACATGT 0.226 0.229 0.111 1.759 1.434 31 9 110692760 —7113 ACAGGGCCTCRAATTCTTCCC 0.520 0.512 0.247 0.319 1.111 31 9 1107152466833 7114 GTATAAAGTAYGTGCAAACAC 0.224 0.226 0.249 0.327 1.014 31 9110737318 7851666 7115 TCTTCTTAGCYGGGAAACTAG 0.872 0.896 0.562 0.3000.939 31 9 110743076 — 7116 AGGAGAAGTCRTGGCCTAAAG 0.017 0.017 0.5380.298 0.289 31 9 110746832 3780528 7117 TACTGGAGCAYGGACCATTTC 1.0440.995 0.565 0.278 0.312 31 9 110761555 2805386 7118CATATCCAAARTGCTTCGCTG 0.389 0.392 0.261 0.213 0.346 31 9 1107695832254841 7119 ATAGTAGATGRTTTAGTTACT 0.354 0.357 0.279 0.467 0.479 31 9110779435 6477795 7120 ATTTATGCCARATAAGTATTT 0.018 0.018 0.059 0.3800.494 31 9 110786780 7866715 7121 GGATAAGAACKCTATATTTCA 0.129 0.1270.315 0.561 0.452 31 9 110806340 10817125 7122 GCTAGGGATCYTAAAATACTT0.120 0.117 0.397 0.471 0.434 31 9 110812487 10817126 7123GGGAAAAATGYCCCTTGTTCT 1.397 1.309 0.647 0.453 0.280 31 9 1108191934366150 7124 CAGAAAACACRTGGGAAAAAG 0.529 0.521 0.885 0.437 0.453 31 9110825551 2192591 7125 ATGATAAGTGMAAAACAGGTT 0.632 0.662 0.996 0.4540.271 31 9 110832787 10817128 7126 GTAGTATAGCMTTTTCTCCAC 0.704 0.6460.492 0.428 0.476 31 9 110843884 2192593 7127 GAACAAGATTWAGCTTTTAAC0.313 0.309 0.341 0.686 0.436 31 9 110852489 10759480 7128TCAGACAATTWTTTTTGGTAC 0.320 0.309 0.142 0.299 0.494 31 9 110859197 —7129 TCGAGTCTGASTTTTTTGAAG 0.130 0.131 0.200 0.553 0.587 31 9 110862610— 7130 AGATCAGGAGSCTGGAGTCCT 0.080 0.081 0.145 0.410 0.838 31 9110871972 6477803 7131 TGAAACTAAAYTGACAGCGTC 0.952 0.902 0.557 0.3140.857 31 9 110882740 10980705 7132 TCCATAAAACYTAGCTCATTA 0.078 0.0790.580 0.381 0.738 32 10 19899999 3852478 7133 TTTGAGTCTTRTGTTTTTCCA0.064 0.066 0.695 0.337 0.347 32 10 19905763 11010520 7134ATCCTCCCCAYAGGAAACCAC 1.920 2.100 0.609 0.374 0.225 32 10 1991630112249318 7135 ATATCACTGCRTATGACAATG 0.370 0.373 0.845 0.623 0.326 32 1019924660 11010622 7136 GGGAGATGCCRTTATGTGTTC 0.188 0.189 0.919 0.5880.478 32 10 19935038 3844359 7137 CATTTTCAGARTTAATTGTGT 0.640 0.6420.399 0.607 0.586 32 10 19942794 3904905 7138 GATCTTTATGYTCACCTTTAC0.197 0.189 0.349 0.956 0.688 32 10 19951897 12261476 7139ATTTGGAAAARGGTAAAATTA 0.876 0.847 0.379 0.547 0.606 32 10 1995852612219684 7140 CCAAGCTTCAYGGGGTAGCCG 0.228 0.240 0.497 0.592 1.002 32 1019964003 10827695 7141 TTGGATCAAAYCTTTTATAGG 0.233 0.245 0.785 0.6571.534 32 10 19976791 7474617 7142 ACATGACACARCTTTTTTCCA 0.937 0.9490.635 0.481 1.417 32 10 19986172 — 7143 TTTTGCTTTGYGGACTAATCT 0.8670.807 0.714 0.957 1.484 32 10 19994710 — 7441 TTGCTCATCTRAATTAATCCT0.515 0.519 0.604 1.247 1.559 32 10 20004919 7916152 7145TATCCTTAAARGTAAAGATGA 0.410 0.415 1.055 1.795 1.359 32 10 200149364748608 7146 TATCTGAGTCRTGCAGCACCT 0.047 0.047 1.485 2.083 1.581 32 1020019440 16919291 7147 CTCTAAATGGRTGACGAGGTT 2.013 1.993 1.911 1.8711.583 32 10 20031847 12256382 7148 TTGAAGTAAAMTTTTGTATTG 1.813 1.7732.181 1.514 1.828 32 10 20042742 2025789 7149 AGTCTGGTTCYGGAGTTAGCA1.610 1.603 2.324 1.735 1.943 32 10 20043985 2478746 7150AAATATGTACRTGCACTCTTT 1.168 1.178 1.652 2.041 1.836 32 10 2005032616919309 7151 CAACTCAGATYCACCATTAAT 0.441 0.452 1.471 2.369 1.773 32 1020057858 7100283 7152 ACACAACTTAMTCAAATTAAT 0.155 0.157 1.320 1.9101.667 32 10 20069776 7073601 7153 TGGTCTGGCTYTTTTAGAGGT 1.354 1.3041.220 1.548 1.741 32 10 20106875 4748610 7154 CTCAGTGCTTYGATATAGGAG1.144 1.210 0.936 1.180 1.887 32 10 20119156 12763948 7155GGTCTTTCAAYTGAAACTAGA 0.740 0.732 1.132 0.702 1.467 32 10 2012540512263244 7156 CCTATCCCCARTTTGATACTA 0.018 0.018 0.709 0.617 0.950 32 1020131169 16919362 7157 CAGCAGTCAGYATTGAATGGT 0.444 0.450 0.252 0.8010.667 32 10 20140825 11011581 7158 CACTCTGCATYGTACAGCATA 0.544 0.5390.152 0.440 0.618 32 10 20150341 11011636 7159 GACGTGTTGTYTTAGAATTCT0.083 0.085 0.382 0.199 0.516 32 10 20159308 12263862 7160TTCAGGTCAGYATCTACCATG 0.292 0.296 0.283 0.147 0.727 32 10 20170064333693 7161 TCCTTCTCTTYAAATCTTACA 0.781 0.746 0.146 0.345 0.581 32 1020176365 7900249 7162 GTCATTATCTSTGGTGGAAAA 0.135 0.141 0.237 0.2490.657 32 10 20182706 16919431 7163 GGTGCTTGGTKCCACAGGTTG 0.147 0.1470.484 0.361 0.631 32 10 20189724 7895714 7164 TCATTCTCTTWTGCGTTGCAA0.455 0.463 0.211 0.553 1.075 32 10 20196780 17756750 7165GTGTGTATGTRTAAATGTACC 0.970 0.924 0.570 0.975 1.165 32 10 20203412752319 7166 AATCTTGTTCRTTGTAAAAGA 0.019 0.020 0.837 0.923 1.131 32 1020209606 11592226 7167 GTATTCAGTAYTTTCCATGAG 1.048 1.045 1.219 1.4041.096 32 10 20216969 11011658 7168 ACACGCCTATYACTCCACTTC 0.716 0.7041.178 1.634 1.105 32 10 20222872 17757908 7169 TTAGGGGGTCRACCATGCTAA1.482 1.494 2.045 1.553 0.898 32 10 20234345 12774164 7170TATTATTTCTSCTAACTGGGT 0.587 0.620 1.962 1.298 1.037 32 10 20241326965787 7171 TAACAAAATGRGCTAGAACTT 1.561 1.522 1.645 1.396 1.125 32 1020249001 1887035 7172 GTATTTGCTAYCACTTTACCA 1.012 0.984 0.908 1.0761.142 32 10 20256253 17758689 7173 CCGGAGGAAGYTGACATTTCA 0.279 0.2780.742 1.041 1.055 32 10 20263456 9651367 7174 TGCGATCATTRCATTGGTCAC0.023 0.023 0.158 0.677 1.189 32 10 20276277 7898627 7175TGGGAAACACRCCACTGTTGA 0.189 0.195 0.095 0.691 1.225 32 10 202836481926204 7176 AGATTGCTCAYATAGAGAACA 0.101 0.093 0.189 0.397 1.019 32 1020290990 1409341 7177 AGTGACGATAYTTATTTGTGT 0.737 0.756 0.422 0.2841.101 32 10 20298993 7922844 7178 CTCATGCCTAYGTTCTTGAAC 0.564 0.5510.518 0.506 1.035 32 10 20313589 1111367 7179 GGACCTCAGAYAGTATAAGTT0.691 0.671 0.728 0.501 0.721 32 10 20317089 4311960 7180ACTATCCTTTYGTTAGAATTT 0.478 0.472 0.860 1.062 0.668 32 10 20331025957210 7181 CAATCTAGAAMTTTTGGATTT 0.561 0.545 0.650 1.192 0.629 32 1020340113 — 7182 TATGTTGAACRAAGGGCTCAG 1.078 1.074 1.323 1.005 2.177 3210 20345947 10764184 7183 TACTTAGCTARTTTCCTCTCT 0.019 0.019 1.239 1.1702.345 32 10 20351919 4311961 7184 GAGACAAACAYTGTGGCAGTG 1.821 1.7761.064 0.894 2.459 32 10 20359898 10740960 7185 AAATGAGACARCAAATGGAGA0.289 0.282 0.895 2.936 2.216 32 10 20366165 17688735 7186TAGAAATCTCRTACATATTCT 0.394 0.379 0.895 2.660 1.923 32 10 203765012358915 7187 CATTATTTCCWAAGTGTGTCT 0.793 0.796 2.557 2.224 1.598 32 1020387770 9664354 7188 AGTTCTGTCARTGGAAAATTT 0.185 0.184 2.623 2.0621.628 32 10 20406431 12250505 7189 ATGGGGTTACRATGGGGTTAG 4.736 4.2672.643 1.439 1.725 32 10 20413892 7917917 7190 TTTTAATATGRTCTCAGTGGC0.563 0.533 2.205 1.295 1.600 32 10 20419570 11011792 7191GTAGAAAAAARTTACAGAAAA 0.298 0.284 2.029 1.429 1.789 32 10 204306472358839 7192 TGTGGGATTAKGTTCTCCTGA 0.268 0.272 0.088 1.553 1.484 32 1020438674 2460593 7193 CCATTGCAATRAATCTTTAGA 0.004 0.004 0.146 1.8231.381 32 10 20445668 2461939 7194 AATTTGAAGTYAGAATTGTTG 0.059 0.0580.389 0.682 1.426 32 10 20454519 2884565 7195 AGGTTTGAGCSTGACCATAGG0.797 0.837 0.723 0.676 1.347 32 10 20464632 16919903 7196TATATTGGTAMTTTTATGAAC 1.030 1.064 1.148 0.622 1.339 32 10 2047163412217582 7197 CTGCAAACATRTAGACTTTAT 1.121 1.087 1.491 0.715 0.476 32 1020477435 16919952 7198 CAAACTGACARAGTAAAGAAT 0.961 0.940 1.060 0.8190.459 32 10 20483092 7895366 7199 CAGCTTCTTCRTATTAAGTTG 0.558 0.5330.803 0.832 0.554 32 10 20495937 2358856 7200 CAAACGTAGARTTCAGAGAAA0.014 0.015 0.485 0.730 0.509 32 10 20504910 11593311 7201AAACCCAGGCRAGCACATTAG 0.539 0.557 0.201 0.547 0.770 32 10 205152162358871 7202 AATTGAAGGCRTCAAAGAGAG 0.377 0.366 0.121 0.427 1.501 32 1020522577 17699919 7203 GGATAAAAGGRGTGTGCTCTG 0.090 0.089 0.228 0.1991.702 32 10 20528200 10508617 7204 GAGACAGAGGWTACTTACTAC 0.000 0.0000.307 0.313 1.529 32 10 20536276 11011870 7205 CTGGGATTAAYTTAATAATTT0.000 0.000 0.190 1.242 1.299 32 10 20543273 16920111 7206GACCTAAATAKGTGATGTTTT 0.700 0.701 0.707 1.605 1.122 32 10 205489456482103 7207 CCTTTTTAGGMCATTGTTTAT 0.090 0.090 1.680 1.653 1.190 32 1020555548 11597607 7208 CATCAGGATAYACTTTTTCCC 1.117 1.012 1.970 1.6941.217 32 10 20562077 1930227 7209 TGTATAGTTGSACGAGTTGTA 2.464 2.4961.756 1.557 1.141 32 10 20564148 10827998 7210 TGTCCTTGCTYTCACTCAGCA1.410 1.407 1.917 1.482 1.102 32 10 20574551 2778978 7211TTTTCAGTTCRAGCTTTCTGA 0.874 0.871 1.662 1.344 1.052 32 10 205863432681951 7212 TTACAGTTAAYGGAGGAAACT 0.582 0.561 1.051 1.429 1.098 32 1020595556 16920183 7213 AGTAAATAACYGGAGATTAGG 0.226 0.231 0.566 1.2190.993 32 10 20601545 2681942 7214 AAAGCTATGTRTTCCCAAGTA 0.906 0.8610.242 0.512 0.928 32 10 20607483 2681947 7215 AAATAAATTTRCGGCATTCTG0.086 0.087 0.129 0.325 1.074 32 10 20614437 — 7216TAAAATGGGAWCTAGAAACTG 0.070 0.074 0.094 0.162 0.938 32 10 2062013110828003 7217 ATCGTCCTCTKCATGTCTTCA 0.192 0.201 0.058 0.132 0.582 32 1020627631 10508620 7218 TGTCATCTACRCATCTATTTC 0.049 0.046 0.089 0.1930.312 32 10 20637777 10764218 7219 ATTTCGTGAAYTGAAGAATGC 0.766 0.7630.181 0.090 0.495 32 10 20648041 12262812 7220 GGCACCCAGTRATGTTCCTCC0.184 0.170 0.294 0.250 0.458 32 10 20673489 11011990 7221TTGGATCATTYTTTTCATGTT 0.442 0.435 0.412 0.263 0.559 32 10 206798471412558 7222 AATCGGGACARTTGTGAAAGT 0.612 0.569 0.462 0.722 0.407 32 1020686226 4748669 7223 ATCCTGAGAGRGAGAAAAAAG 0.374 0.364 0.434 0.9290.588 32 10 20718364 — 7224 GATTTTTTTTWTTCGACCAAA 0.917 0.856 1.1270.870 0.640 32 10 20724009 12777485 7225 GAAAAACCCARCCATCTCATA 0.1390.137 1.035 0.852 0.638 32 10 20735677 12783104 7226TGGCACTGAAYACCGACGCCA 1.773 2.151 1.195 1.036 1.062 32 10 2074256617783778 7227 AACTTCATACRTTCTGATATC 0.426 0.429 0.804 0.921 1.046 32 1020747068 16920492 7228 TGAATACATCYCTGATATAAG 0.735 0.730 1.219 0.8601.024 32 10 20760926 11818220 7229 AGATCTATCCYAATACACTGT 0.170 0.1710.489 1.023 1.119 32 10 20783154 10828054 7230 CATAAATAAGKGAAAATTTTT0.874 0.885 0.387 1.311 1.177 32 10 20786206 12769394 7231TCCTCAATTCRTGATTAAAAC 0.295 0.281 0.695 0.676 1.494 32 10 2081244116920534 7232 TGAATTAGAGRTGGTAGCTCA 0.251 0.227 1.007 0.764 1.293 32 1020821183 12262779 7233 TGACAATTTGYATGAGAAAGA 1.412 1.398 0.609 0.7861.356 32 10 20830242 12782055 7234 TTCTCCTTGGYCCCTGGAAAA 0.773 0.7510.809 1.256 0.761 32 10 20835803 1361360 7235 AAGAGTAGTTYTGTTCACTTA0.066 0.101 1.161 1.059 0.873 32 10 20848770 7913924 7236AAATGAGGATWGTAATATGTA 0.656 0.645 1.061 0.953 0.692 33 10 999667297078393 7237 AGTTTGCAAGRTAATATGTTC 0.133 0.130 0.250 0.572 0.947 33 1099982196 11189521 7238 GTATAGCTGAWACTTATATAG 0.033 0.035 0.395 0.9070.630 33 10 99989333 10748721 7239 ATGCTAAGTCYTCCATGTTTT 1.250 1.3550.901 0.739 0.648 33 10 99995272 10786405 7240 GTGCCCATGAYGATGATGAAT0.450 0.454 1.392 0.805 0.664 33 10 100006303 1983866 7241ATTTCTATTCWTCTTTCCTGC 1.542 1.632 1.485 0.964 0.718 33 10 10001384711189527 7242 AGCTTGGATASCCTGTGATAG 1.530 1.590 1.175 1.144 0.659 33 10100021384 10786408 7243 GGAACGTAGAYTGTAATAACA 0.029 0.030 1.286 1.2420.895 33 10 100026199 7915743 7244 TCTGAATTGCYGTCACTGTGG 0.296 0.2990.806 1.002 0.973 33 10 100037491 10883059 7245 CATGTTTGAGYGAATGGCCTG0.553 0.612 0.225 1.051 1.027 33 10 100047807 7903057 7246CTTTTTATTCYGCATGAGTCT 0.569 0.569 0.346 0.870 1.067 33 10 10006054717453254 7247 CTTAAAAATCMCGGAGCTAGA 0.277 0.289 0.408 0.379 0.752 33 10100071747 2862297 7248 ATGATAGTAAMCCAGGTGCAT 0.341 0.350 0.631 0.3900.770 33 10 100086156 4919215 7249 TTCAACATTTYTGTCCCTGAA 0.000 0.0000.501 0.300 0.341 33 10 100092393 11189558 7250 TCTGTGTGGCRATTGTTGAAA1.094 1.015 0.415 0.210 0.194 33 10 100105017 4919221 7251GAGATCCCATYAGCTCTCCTC 0.321 0.329 0.269 0.166 0.328 33 10 10011637617109506 7252 TCACGTAGCCYTCACAACACA 0.115 0.115 0.221 0.142 0.280 33 10100125512 17109560 7253 TTTCACTCATYATGTCTAGTG 0.007 0.007 0.043 0.2880.238 33 10 100132112 17109594 7254 GGTGCCAGGTYAAAAAGTATA 0.262 0.2560.017 0.355 0.263 33 10 100138532 2274248 7255 AGCTCCCAAAYGCAAATGTCT0.000 0.000 0.186 0.120 0.442 33 10 100149203 11812682 7256AGGGCCCTTAYGGAACTCAGT 0.170 0.169 0.461 0.125 0.380 33 10 10015738612763326 7257 GGTAGGACGCRCGGGAGAACT 0.879 0.834 0.378 0.239 0.343 33 10100165072 2274245 7258 GCTGGGCCGTYGGGAGTGTTG 0.655 0.613 0.357 0.5830.170 33 10 100172275 2296435 7259 AGGTCTGAGTYAAGGTGCTTA 0.035 0.0350.543 0.525 0.122 33 10 100181485 11189602 7260 GAGATGGGGASAGAGTTTGCA0.426 0.423 0.707 0.459 0.133 33 10 100190787 7071947 7261TGAGAACACGRGTGATGTTGA 0.680 0.684 0.428 0.443 0.265 33 10 10019522717109873 7262 TTGAGAAAGGRCAAGCTGGAG 1.183 1.122 0.526 0.199 0.367 33 10100205957 7093411 7263 CATGACCACCRGAGGCTAAGT 0.011 0.010 0.399 0.1200.328 33 10 100216639 17109945 7264 CTGATCTTAGRACATTGTCTG 0.251 0.2580.159 0.287 0.387 33 10 100230295 17458353 7265 AGCTCCAAACRGCAGGGGAAT0.196 0.162 0.009 0.399 0.414 33 10 100241958 2017304 7266ACATTTGGAARAAAGAGATAT 0.002 0.002 0.121 0.294 0.392 33 10 1002560821932796 7267 TTTTATTTTTRGGAGTTTGCT 0.191 0.181 0.286 0.135 0.549 33 10100266875 17537531 7268 AGATCCTAAGRCCCAGAGCAG 0.847 0.823 0.356 0.3620.567 33 10 100275239 12412681 7269 GCTGGGAAGAYGGGCCTAAGG 0.776 0.7630.500 0.433 0.419 33 10 100282154 7096013 7270 TTCTGCCTCTSCTTCGTGATC0.289 0.278 0.868 0.575 0.265 33 10 100283172 665142 7271TGTTTCGTAAYGTTAATCACA 0.410 0.430 0.698 0.754 0.353 33 10 10029770511189671 7272 GCAAGAGGTCWCTTCTTCTAC 1.007 0.989 0.669 0.718 0.459 33 10100305542 10509724 7273 TTTGACAACAYGAAAATAACG 0.519 0.505 0.791 0.5690.443 33 10 100316583 544176 7274 TAGTTCCTGTYACTCCAGATG 0.649 0.6960.599 0.478 1.423 33 10 100332431 11189687 7275 CTTTTATGGCRTAACTGTCTC0.506 0.489 0.338 0.639 1.655 33 10 100340328 10883146 7276AGCCATGATGYGCTTATCTGT 0.022 0.022 0.332 0.531 1.715 33 10 100351260560749 7277 ACTACCAACCSTGATTATCTC 0.395 0.404 0.362 1.517 1.732 33 10100359992 526465 7278 TCCACACCATRGCTTGGCAGT 0.506 0.505 0.216 1.8031.916 33 10 100370950 10883151 7279 ACTAGTCCAGKTATATTGTAA 0.735 0.7732.104 2.036 1.730 33 10 100379797 11189719 7280 GCTAACTGCTWTATTTAACTT0.101 0.103 2.161 2.037 1.461 33 10 100389254 17110455 7281CATCTCTCAGKGGCTCTACTG 3.465 3.488 2.680 2.241 1.644 33 10 100396734563060 7282 TAAACCTGTAYTCCAGATCCT 0.926 0.932 2.753 1.974 1.465 33 10100402147 528556 7283 TTCAACAACCRGTCCCACATA 1.021 1.037 2.855 1.7331.608 33 10 100409703 475945 7284 ACTTCACTGARTACTTGTGAG 0.980 0.9730.884 1.823 1.781 33 10 100424345 3021452 7285 TCTCAAGATTRTGTATAAAAG0.464 0.451 0.632 1.708 1.772 33 10 100425944 965934 7286ATGAGTGGCCRGTGTTATTAA 0.017 0.017 0.702 0.946 1.829 33 10 10044567510748750 7287 ACCAGCTCTTRAATGAATAGA 0.394 0.395 0.362 0.893 2.177 33 10100455887 17110699 7288 CTCAAATTCAYGTAGTTAGTG 1.229 1.221 0.524 0.7082.207 33 10 100463182 17110721 7289 ATCCTCCAAAYTGTAATTCTT 0.011 0.0110.880 0.582 1.212 33 10 100471417 17110744 7290 TCTAGGAGGGYGATGACATTC0.942 0.945 0.873 1.133 0.991 33 10 100483316 17110762 7291TGGTACTTACKGCTGCCTTTA 0.712 0.657 0.521 1.330 0.843 33 10 10049188111189793 7292 GGAATACGAARAACTTCAGGC 0.408 0.396 1.559 1.225 0.715 33 10100500931 10786467 7293 TTGACAGAGASGATTAACATT 0.549 0.542 1.289 0.8160.962 33 10 100524479 17110835 7294 TAAGTAACCGRTACTGGCAGC 2.011 1.9020.991 0.925 1.202 33 10 100530404 17110853 7295 AGGCTTTTGAYTGCTTGGCTA0.494 0.488 0.900 0.746 1.189 33 10 100541536 1414971 7296TGCCTGACATWCCACAGCTAG 0.203 0.203 0.797 0.997 0.853 33 10 10054244610883202 7297 GGGGTCAGAGYGGTGGTTGTT 0.109 0.110 0.162 1.194 0.876 33 10100566461 10883210 7298 AACTGCCTCCRTAAACAACTT 0.383 0.376 0.480 1.0580.761 33 10 100574864 10786475 7299 TGTAATACTTRTAACAAAAGA 0.389 0.3930.703 0.394 0.736 33 10 100581061 17110919 7300 AGTGTGCTTCYTCCAAGGCAA1.391 1.409 0.857 0.325 0.742 33 10 100587657 7084345 7301ATATCTGTGCRGTTTTGCTTA 0.648 0.669 0.677 0.422 0.779 33 10 1005936852862447 7302 TATTGCTTCARTTCTAATTCT 0.407 0.400 0.580 0.555 0.393 33 10100607163 7091295 7303 ATTGTGAGTTSGAATAGAAAC 0.045 0.047 0.307 0.5520.354 33 10 100610499 10883221 7304 TAAGGCCTAAYTAAAATGCCA 0.169 0.1710.281 0.644 0.451 33 10 100631865 10786481 7305 CCTTTCTCCAYTAGATAAGCA0.550 0.549 0.231 0.335 0.537 33 10 100638398 4919262 7306CTCAATTACCRTCTCCTTTCT 0.606 0.599 0.438 0.238 0.510 33 10 100650291978851 7307 TAGTTATGCAYGAACATTATA 0.290 0.337 0.494 0.292 0.506 33 10100656085 — 7308 CATGGAGTTTSATTGTGAAGA 0.404 0.680 0.348 0.407 0.430 3310 100662056 17110998 7309 CTTCCAGCAAYGTCATTTGGT 0.324 0.293 0.391 0.4240.407 33 10 100684372 11492738 7310 TCTGGCTTGCRGAGTTTCTGC 0.273 0.2750.475 0.360 0.470 33 10 100695326 17111002 7311 CAGAAAATACYTGCTATATAA0.654 0.618 0.278 0.507 0.784 33 10 100702951 4444007 7312TAAGATGTTCMATAAAAGGCT 0.482 0.472 0.303 0.583 0.787 33 10 10070936211189907 7313 TAAACTCCAGYAAAATCTCCC 0.256 0.250 0.627 0.594 0.680 33 10100724399 2902256 7314 CGTTCCTACAWAAAGATCGTG 0.358 0.364 0.568 0.9420.654 33 10 100729760 17111026 7315 TCAGATGAACRCTGCAGGCCA 1.026 1.0080.694 0.895 0.638 33 10 100749687 10509727 7316 TGCAGTATGAYAGATGAATTA0.546 0.543 1.258 0.698 0.721 33 10 100759845 12249519 7317CCAATAAACCRATAATCTAAA 0.735 0.726 1.126 0.727 1.418 33 10 10076821110509728 7318 TGTTGGACAAMCTTTGGCTAA 1.323 1.295 0.666 0.723 1.468 33 10100789378 2487890 7319 TTTGCAGATASAGAGTAAGTC 0.178 0.182 0.665 1.0221.420 33 10 100797780 2862507 7320 GCAATTAATAWGCTGTGGACT 0.130 0.1280.428 1.820 1.405 33 10 100814705 11189954 7321 CCTCCTCTACYATTATTCTCA0.000 0.000 0.269 1.689 1.793 33 10 100819181 1342576 7322AATTACTGTAYAGATTTTGGA 0.239 0.234 1.751 1.641 1.716 33 10 1008319154462267 7323 AGGGTAGGGGYCTGCTACATG 1.001 0.992 1.758 1.142 1.539 33 10100841378 10883258 7324 AAATATGCTCSATTTGGCATT 2.627 2.974 1.758 1.6141.373 33 10 100848616 10509729 7325 TAAAGTGCTASGGTTCTCATA 0.602 0.6141.676 1.412 1.131 33 10 100859904 7089572 7326 TAAAAGAAAARGACATGCTCT0.000 0.000 1.922 1.415 0.877 33 10 100871811 1857184 7327ATTATGTATAYGTATGTGCTT 0.261 0.320 0.719 1.365 1.879 33 10 10089044511189991 7328 GACAACTGAAYTCGAACCCAT 1.539 1.525 0.886 1.087 1.712 33 10100895445 1935665 7329 TTGACACCAAYGATTTTGGAA 0.070 0.068 0.717 0.5011.611 33 10 100939639 3905193 7330 TACCAAGTTGWTGCTGTTCTT 0.998 1.0110.609 1.691 1.589 33 10 100968381 10786524 7331 AAAACCCCAARTGAAGACATT0.171 0.171 0.158 1.431 1.486 33 10 100973931 10748766 7332CACTGTCTTTYACCATATCAA 0.008 0.008 2.037 1.287 0.892 33 10 10097990912573571 7333 GTCTGGAAGGMCTGACTTTAA 0.176 0.177 1.619 0.930 0.902 33 10100989074 17094130 7334 GTAAGAAAACRTGAAGATTAA 4.480 4.422 1.642 1.1050.823 33 10 100996728 10786525 7335 TTCTCAAAAAMAAAAATAATC 0.114 0.1081.757 0.780 0.828 33 10 101031490 12570965 7336 AAGATTGTTARGCAAGGAACA0.044 0.043 2.086 0.893 0.729 33 10 101041410 17111428 7337ATCCTATCTCRTTTTTTCTTT 0.077 0.069 0.010 0.998 0.779 33 10 101060139 —7338 ATTGATTAGTKTTTCCTGTGT 0.334 0.330 0.035 1.107 0.665 33 10 10107137510509733 7339 GAACCATTGCYCTATAAAGAA 0.058 0.056 0.056 0.099 0.652 33 10101083674 11190059 7340 GTATTTAGGTRGAGCACTAAA 0.440 0.456 0.100 0.1020.865 33 10 101090322 4919300 7341 TAGTACTGTCWTAAAACGAGT 0.187 0.1860.324 0.152 0.946 33 10 101096097 12570708 7342 ACTTCATTACRTGTGAACACT0.337 0.333 0.382 0.158 0.212 33 10 101108239 10509734 7343TCGTTTCAGAMTTTGGTCCTT 1.034 1.033 0.360 0.245 0.220 33 10 1011155911892509 7344 AATTATCAGARGGTCCCAGAG 0.208 0.207 0.320 0.374 0.502 33 10101123989 4919305 7345 GTTCTCTACAKTACCATCACT 0.393 0.388 0.495 0.5580.792 33 10 101134457 17111633 7346 TGCTTAAAAARAGGGTTAGAG 0.086 0.0880.290 0.537 0.775 33 10 101140337 3793932 7347 GTTTTGGGATMTGTCCCATTC0.768 0.767 0.645 0.909 0.985 33 10 101147807 3750897 7348TTAGTTCTTCSCTAACCTGGA 0.497 0.500 0.522 1.015 0.870 33 10 1011601902862611 7349 CTCAACTTTTMACTCCACCCT 1.079 1.043 1.099 1.055 1.036 33 10101168146 11594790 7350 AAACTCCCCARCATGGAATCT 0.129 0.130 1.477 1.1291.111 33 10 101178468 4919310 7351 GTATACATCCSGCTGCAAAGA 1.621 1.6691.433 1.106 0.973 33 10 101188327 2494654 7352 AGTCACATCTRTTATATAGTC1.355 1.325 1.361 1.200 1.001 33 10 101203270 2494657 7353ATCCTGGTTAYGAATAGTCAG 0.157 0.155 1.367 1.404 0.907 33 10 10121046417111730 7354 AGGCCCTGACRTAATTTCAAC 0.741 0.759 0.927 1.182 0.968 33 10101217569 10883350 7355 AGATAAACCCYTGGGCAGCAA 0.192 0.194 0.658 1.2530.854 33 10 101219517 2862596 7356 ACTTGAAAATYTCTATACGTC 0.952 0.9780.753 0.664 0.835 34 11 11697651 1471014 7357 AATACAATGCYAAGTTTTAGA0.887 0.848 0.157 0.371 0.281 34 11 11704216 2233743 7358GTCTCCTAACYACTCAGTCTC 0.180 0.172 0.310 0.128 0.289 34 11 117107261455252 7359 ATGAATCACARTATACCAATA 0.410 0.388 0.484 0.124 0.710 34 1111717646 17446021 7360 AGGTGGGAGGYGTGAAATCAG 0.582 0.542 0.188 0.1110.628 34 11 11723285 11022014 7361 ACCAGTGGGAYGTAGCTAAAA 0.482 0.4920.160 0.198 0.488 34 11 11730217 897362 7362 ACAAGATAAAYGGATTTGGAA 0.0140.014 0.092 0.775 0.568 34 11 11737183 16924665 7363AAAAAGCATAYGTAATCCTCT 0.177 0.172 0.082 0.798 1.089 34 11 1174675716910054 7364 AATCAGTTTTRTGGAGTGCAT 0.101 0.093 1.028 0.885 1.111 34 1111756544 1455264 7365 TCTTCATAAAYGTTTCTGACT 0.518 0.493 1.110 0.8771.113 34 11 11763136 16910116 7366 AAGCTTGTCTRCATGGGGGCT 2.970 2.7401.363 1.474 1.812 34 11 11770132 7940987 7367 ATTGTTTTCTWCAATCATAGC0.122 0.122 1.701 1.470 1.762 34 11 11781525 2054115 7368TTAACACCTCRAGAGATACAG 0.676 0.642 2.480 1.918 2.190 34 11 117878077120182 7369 ATATATGGAAWGAAAGCCTTT 0.459 0.467 0.888 2.936 2.151 34 1111793828 12575179 7370 TCTGTGATTAMCATGACTTAA 1.882 1.898 1.447 2.8862.321 34 11 11801092 1542379 7371 TAGATGTTAARTTCATACTTG 0.132 0.1362.043 2.055 2.936 34 11 11807368 7944875 7372 GATGATAATTRGGTTTAAAAG1.003 0.975 1.889 2.195 2.740 34 11 11821720 7926410 7373TGTGTATTTCRTTTTAGAGCT 2.262 2.012 1.645 1.987 2.787 34 11 1182432617378517 7374 TCTCTTTTAAYAGCATCCCAA 0.314 0.314 1.863 2.412 2.772 34 1111850597 7935751 7375 GGAAGCAAGTYAAAGAATTTG 1.995 1.952 1.754 1.7243.062 34 11 11873223 7111921 7376 ATTAATATTGYTGATATTATT 0.000 0.0002.182 1.870 2.767 34 11 11879369 4243945 7377 CAAAAGAAAARAAAAAGTCTT0.002 0.002 1.976 2.905 2.481 34 11 11887874 4910429 7378TAATTTCATAYAGTGCCTTTG 2.977 3.180 1.258 3.233 2.264 34 11 119117946485303 7379 TGAGACATAAYTGGTGTCAAT 0.051 0.052 3.108 2.932 2.320 34 1111930555 16910238 7380 ATAGGAGCCARTAGAGAAACA 0.453 0.464 3.687 2.5332.556 34 11 11936250 1979687 7381 ACTTGCTATCRCACAAAAGAG 3.982 3.3752.819 2.533 2.456 34 11 11944883 7480000 7382 AGCATCAACASGGGAAAAAAA2.609 2.458 2.689 2.547 2.418 34 11 11951408 1552796 7383GGACTGGAGCYGACAGACACT 0.708 0.708 2.653 2.178 2.153 34 11 119576124757519 7384 CCCAGGAGATRAGTGCCAGAG 0.486 0.478 1.354 2.378 2.049 34 1111964354 7113678 7385 ATTATGAGACYATGGAAAATT 0.000 0.000 0.478 2.2702.125 34 11 11971244 987663 7386 TCTAGCATTTRCACTGAAAGT 0.087 0.090 0.2090.798 1.523 34 11 11977280 17380453 7387 TTACAAGTCCRGGTGTCTAGT 0.6880.680 0.118 0.169 1.662 34 11 11983640 6485369 7388TGGTAGCATAYAGAATAGTAA 0.108 0.105 0.058 0.112 1.631 34 11 119980724500466 7389 TTCCTCTATCRTTGTCATGCT 0.147 0.143 0.138 0.116 0.749 34 1112008425 4757082 7390 CCCATGGAAGRGCAAAAGGCA 0.058 0.054 0.083 0.1960.227 34 11 12020875 16910400 7391 TTAGTAAATAYGCTGACTTCC 0.405 0.3750.151 0.189 0.138 34 11 12029564 1994328 7392 CTCTCTGAGGRTGAAATGAGA0.442 0.444 0.329 0.212 0.089 34 11 12040379 11022142 7393GAATCTCTAAYGGACTGAGGA 0.527 0.480 0.348 0.220 0.068 34 11 120500342018347 7394 TACCATGTGCRGTTACTAATG 0.742 0.757 0.536 0.210 0.150 34 1112056845 10831721 7395 TTTCTCCCCTYATTGGCCTCG 0.074 0.072 0.432 0.2000.081 34 11 12064047 9665967 7396 CTTAAGAAGARCCTAGAGCAA 0.850 0.8190.304 0.161 0.103 34 11 12070720 11022169 7397 TCAGAGCTCCRTGCAGGCCCA0.179 0.185 0.080 0.238 0.123 34 11 12078670 7111481 7398CTAGCGAGGAYGCGAAACTTC 0.083 0.087 0.094 0.166 0.177 34 11 120947847947696 7399 TTCAGAGCCCRAGGGAGTGAT 0.018 0.018 0.091 0.089 0.336 34 1112107131 7940840 7400 TGCCTTCTTARTCTCTGAGGT 0.173 0.177 0.075 0.1360.336 34 11 12112990 16910593 7401 TGGAAGGTTTYGAGGAGGCTC 0.843 0.8280.137 0.073 0.513 34 11 12119025 1009087 7402 AGACTGAGGARGAAGGACAGG0.081 0.081 0.245 0.290 0.519 34 11 12126901 10831742 7403GTGCAAAACCRTCATACAAAC 0.328 0.334 0.351 0.399 0.669 34 11 121323014756783 7404 TGGGGGACTTRGCCCTGACCT 0.398 0.389 0.516 0.808 0.495 34 1112139612 7131034 7405 AAGTTTTGAGMAGAGACTGTT 0.462 0.470 0.688 0.9700.555 34 11 12149786 10831746 7406 CTGCAGGTAAYTGAGATGCTG 1.288 1.2341.163 0.920 0.702 34 11 12159487 7926135 7407 AGAAATTCTCRATTAAAGTCC0.473 0.464 1.340 0.981 0.836 34 11 12172973 10437601 7408GAGGCTATAARAAGAAAATAT 1.281 1.328 1.362 1.018 0.906 34 11 1217961110831761 7409 GTGGGTGCGCRTTCCCTTGCC 0.797 0.801 0.878 1.093 1.235 34 1112186231 2279390 7410 TCGTGAAGGTKCTAGAGCCTC 0.612 0.650 0.874 1.1341.306 34 11 12191983 11022250 7411 TCCCGGGTGAKTTTGTGCAAT 0.115 0.1170.588 0.880 1.380 34 11 12198606 4237704 7412 CGGAGCCCCCRTGTTTCTCTG0.433 0.431 0.478 1.475 1.293 34 11 12212122 2010576 7413ATTTGGGTATRTGTGTTGCTA 0.742 0.682 0.404 0.974 1.468 34 11 122181402706631 7414 ATACAGTTTGMAGGAGCTTTG 0.554 0.567 1.390 0.895 1.430 34 1112224116 11608031 7415 TCCAGCTGACKGGGCCCTTCA 0.000 0.000 1.247 0.6571.222 34 11 12231441 1826608 7416 GTCTCCAAGCYGACCTTGCCT 1.847 1.7701.183 1.069 0.902 35 11 19443295 12792754 7417 ACTTTCCACTYTCCTTAGGTA0.250 0.245 0.293 0.520 0.203 35 11 19452545 2632034 7418GATGTGTACAYTATGATACAA 0.207 0.201 0.533 0.520 0.323 35 11 1946439310833124 7419 ATAGTCTCTTYCTGGGACCCT 0.337 0.328 0.708 0.473 0.246 35 1119467603 11025154 7420 ACAGGCAGATYTGAGTAACTT 1.491 1.473 0.658 0.3080.236 35 11 19475674 2403494 7421 CCCAGGCTCTYTTCCTATTTT 0.862 0.8660.604 0.419 0.350 35 11 19486762 16936838 7422 CTAAGTTCCCYGTCCTGTACT0.037 0.036 0.513 0.351 0.380 35 11 19496685 2702735 7423TTAGAACAAARTACCCTGCCT 0.030 0.030 0.233 0.349 0.338 35 11 195046497127676 7424 TTTTTCACCCRTTGTTTACTC 0.127 0.128 0.033 0.465 0.290 35 1119518021 16936939 7425 AGATAGGTCGYGGAGGGGGAC 0.759 0.827 0.065 0.1720.249 35 11 19523724 11025179 7426 TAGTGCATTTYATTTCCTAGA 0.002 0.0020.257 0.129 0.279 35 11 19531121 11025181 7427 TCTGCAATTGYGGAGTGAAAA0.236 0.234 0.382 0.141 0.361 35 11 19543750 4757007 7428TTGATTTTCCWATGGTGATAA 0.726 0.742 0.315 0.149 0.217 35 11 195504261487199 7429 TAAAGCCCTCMGTCCCTTCTT 0.000 0.000 0.356 0.215 0.137 35 1119556547 10741780 7430 CCTGATGTCAYTGAGCCTCTA 0.599 0.612 0.273 0.1990.889 35 11 19569906 4757821 7431 TTTATCTTGARGTCCTGAAAT 0.114 0.1160.154 0.379 1.099 35 11 19576520 1471903 7432 TAGCTTCAGGKAATTCTTTTT0.029 0.030 0.252 0.415 1.024 35 11 19584103 10833134 7433CATACCGTTTYAGACGGAGGC 0.387 0.391 0.300 1.356 1.118 35 11 1959202917599506 7434 TAAAACCTAARTTGCCTCATC 0.646 0.652 0.392 1.434 1.991 35 1119607973 7116289 7435 TGGAATGGCCRCATCAACCCT 0.773 0.753 1.995 1.1962.735 35 11 19614433 16937060 7436 TACAAAGCGGMTCTTGGTCTG 0.355 0.3652.448 1.618 3.108 35 11 19630248 1559667 7437 CCAATTAAATRAAGCCTATTA3.341 2.951 1.808 2.759 3.108 35 11 19638525 16937087 7438CTGGACATTAKGGTTCATACG 0.625 0.626 2.029 3.375 3.488 35 11 1964797710833153 7439 TCCTTCCTCCYTTGTCCTGGG 0.075 0.074 3.105 3.474 3.665 35 1119653940 2216997 7440 TACAGCAATTKGTAATTCACG 1.141 1.204 3.271 3.6043.687 35 11 19659944 7113050 7441 AGACCTGTGGRCACATCTGAA 2.724 2.8853.322 4.036 3.710 35 11 19663584 752459 7442 AGGTATGGCCRGGCTGAGGAG 3.0293.156 3.676 3.633 3.488 35 11 19687211 890136 7443 CTTATAGGCCRTTTTTATAAT2.155 2.231 3.748 3.467 3.386 35 11 19696994 — 7444CACAAATATAYACACATGGAA 0.000 0.000 3.091 3.551 3.559 35 11 197070311559665 7445 CAAGGAACGTRTGCAGAGAAA 0.631 0.663 1.379 2.883 2.492 35 1119724079 12223569 7446 GATCAGGGCTKACCGTATTTA 0.426 0.396 0.547 2.0002.291 35 11 19731568 16937173 7447 CATATTTGTGKTAGTAAGTCA 0.190 0.1890.384 0.981 2.440 35 11 19738767 7118473 7448 GCAAACGGCAYCTTTTCAAAA0.889 0.862 0.419 0.289 2.540 35 11 19744864 12797612 7449CCACTTGGGAWTGGCAACTAG 0.095 0.097 0.338 0.205 1.622 35 11 197510091425237 7450 AACTGAAATAYGTTATCTGGG 0.687 0.677 0.319 0.207 0.769 35 1119764639 10766594 7451 AGGTCATTGAKATTGCATACA 0.177 0.181 0.073 0.3800.384 35 11 19769834 10833171 7452 ATCCAGAGAGYGGGCACTCAG 0.126 0.1270.202 0.362 0.408 35 11 19781820 10833174 7453 GATTCCCCCARTGGTAGAGGA0.107 0.103 0.369 0.261 0.399 35 11 19790982 10833178 7454TAAGAGGAGASCAGAATTCAG 0.602 0.609 0.337 0.393 0.327 35 11 197952391425235 7455 ATAATCACCAYGAGTGCACAC 1.180 1.113 0.472 0.360 0.368 35 1119812716 1816814 7456 ATGGAAATACMACTTGCATCT 0.144 0.142 0.713 0.4740.210 35 11 19820738 16937196 7457 ACGATGCCCCWTCACTCCAAG 0.479 0.4770.680 0.455 0.274 35 11 19827846 12287043 7458 ACATGTTGCARAACCAAGAAG0.642 0.618 0.452 0.553 0.195 35 11 19842939 2014079 7459CTGGGGAGGTRCATCCTGGTC 0.589 0.567 0.405 0.374 0.189 35 11 198484381838053 7460 ACATGATTTCSTCCTGGTAGA 0.604 0.612 0.380 0.208 0.172 35 1119854870 12803334 7461 ACAGTGGATGWCCCCTGATCC 0.064 0.065 0.176 0.2160.189 35 11 19861136 2625301 7462 TTACATCAGTSGCTCGGTCTC 0.380 0.3930.154 0.166 0.161 35 11 19866977 12418955 7463 GGTCTTTTACRCACTACCTTT0.045 0.043 0.065 0.080 0.094 35 11 19872824 7950256 7464GGAAAGACTTYAGTGTCAGAA 0.489 0.485 0.096 0.037 0.099 35 11 198849202028608 7465 AGCTGTTAAARTAGAACTCCG 0.161 0.157 0.034 0.021 0.098 35 1119894431 10833192 7466 ATGTTCAGCARTGCAGCCCAA 0.180 0.171 0.062 0.0740.050 35 11 19906067 12223116 7467 ACAAGGCCTGRTCATGTTTCC 0.027 0.0270.036 0.057 0.022 35 11 19912076 1372989 7468 CAGGAATGAARAGCATGCCCG0.206 0.209 0.114 0.105 0.019 35 11 19918239 10833202 7469CTTCAGATAARGTTGCTTTTC 0.362 0.345 0.111 0.041 0.023 35 11 1992989111025337 7470 ACCTTTGCTTRAAAGCACCGA 0.618 0.597 0.223 0.028 0.015 35 1119944988 2403547 7471 ACTCTCTCACRTTTCCAACTT 0.173 0.174 0.171 0.0550.023 35 11 19953560 10833211 7472 AATTAAAATGYTCCTGGACTT 0.420 0.4340.090 0.066 0.009 35 11 19959591 10833212 7473 ATTACCCAACYGTGTGTGTTG0.002 0.002 0.071 0.051 0.012 35 11 19966633 1442715 7474TCCACCTTTGWGCCAGGAAAC 0.055 0.056 0.066 0.044 0.018 35 11 199731357111951 7475 GAAGGCACACRTTATTTATTT 0.449 0.467 0.015 0.013 0.072 35 1119980715 16937344 7476 CCGGTTTGGTRGAAAGGAAGA 0.139 0.136 0.046 0.0240.075 35 11 19988441 11605275 7477 TTTAGAGACTYATTTACAAGA 0.075 0.0740.052 0.022 0.074 35 11 19995854 10500867 7478 CTATAATTCTYGTTGAAAATA0.260 0.257 0.029 0.124 0.066 35 11 20006435 4757880 7479CTGAAACTGGRTCTCCTGGTT 0.072 0.069 0.067 0.154 0.074 35 11 2001223912276043 7480 AGTAAAGAGGKTAGTTGGTTC 0.305 0.289 0.332 0.119 0.052 35 1120022249 3802799 7481 TGTCTGACGAKTCCGACAAAA 0.430 0.419 0.338 0.1850.061 35 11 20035072 11025365 7482 TAACACAGCCRTAAAGCTCTA 1.041 1.0200.435 0.220 0.136 35 11 20048972 11025369 7483 AACCCTTTTGYTTAGCAGAAA0.269 0.251 0.538 0.213 0.142 35 11 20056025 12146551 7484ACTTCATTCCYGCTCCTCCTG 0.336 0.332 0.436 0.217 0.540 35 11 2006296010833240 7485 TTTAAACTTTYTGTATAGCAA 0.544 0.513 0.143 0.377 0.538 35 1120069068 1837971 7486 TGAAGCTGACRGATATCCGCT 0.200 0.200 0.114 0.4140.470 35 11 20076082 2289561 7487 GCTGCAATTAYCTAACAAAAG 0.238 0.2260.288 0.778 0.600 35 11 20089989 4757893 7488 ACTCAGTTGARGACCAGAGAT0.107 0.109 0.305 0.698 0.637 35 11 20098415 2246192 7489CCCTGCTTTARTCTCAGCAAA 0.900 0.963 1.169 0.596 0.726 35 11 201161591520905 7490 CCATCTACCAYAAAGCAGCTC 0.576 0.551 1.120 0.612 1.067 35 1120122076 1442717 7491 ATCCTTTCACKCAGGGCTGAG 2.092 2.041 1.093 0.7090.974 35 11 20139355 10833258 7492 AAAAGTAAAASGAGTCTCCTT 0.086 0.0860.892 0.939 0.999 35 11 20153249 16905792 7493 AGAAAGCAAGRGCCAGTCAGG0.030 0.032 0.823 1.843 1.066 35 11 20159301 10833260 7494TAGGTGTCCTYGTAGGTATTA 0.572 0.561 0.236 1.403 1.067 35 11 2016804017232465 7495 AGAAACACATRGTTCAACCAA 0.416 0.415 1.155 1.297 1.291 35 1120178333 — 7496 GTGTATTTGGYGTAAGAAGTT 0.743 0.751 1.227 0.701 1.238 3511 20187177 17298222 7497 TTCAGGAGCAYTGCAACACAA 2.141 2.195 1.118 0.7590.981 35 11 20193972 11025445 7498 TCATGCCAGARCTTTAGAGCC 0.086 0.0841.302 1.025 1.004 35 11 20199867 1356981 7499 TGTAATAATARGTCTAATCAA0.413 0.388 0.977 0.808 0.521 35 11 20205827 1520907 7500CACTGACTTAYATGCCCTTAA 0.616 0.603 0.248 0.790 1.444 35 11 202199771402377 7501 TTTTATTAAGWTATGCCTTGG 0.193 0.202 0.250 0.752 1.455 35 1120225966 1520896 7502 TTGTAAATCCRATGACTATTT 0.621 0.620 0.255 0.2151.342 35 11 20233015 10833271 7503 CTGATATGAARCAATTCATCA 0.058 0.0540.303 1.186 1.332 35 11 20245613 4757917 7504 GCCCTACCACRTGTGCAGGTT0.369 0.363 0.324 1.099 1.336 35 11 20256439 2403577 7505CTGTTATTGCYTTTCACTGTA 0.680 0.698 1.463 0.962 0.784 35 11 2026379517232549 7506 GATTCCATCCYGTTGACTTTG 0.340 0.336 1.492 1.020 0.813 35 1120269195 12804805 7507 ACCTGAGCCCSAGATTTTGGT 3.032 2.714 1.370 1.0971.304 35 11 20275658 10833282 7508 TTTGGCTGATRATTTTAGGCA 0.249 0.2501.307 1.121 1.262 35 11 20285058 7106374 7509 AGCTTCCTCARTAGGAAAGGT0.427 0.441 1.671 1.057 1.782 35 11 20290488 16906035 7510TAACAATAGCYAATATTCGTA 0.211 0.208 0.309 1.775 1.754 35 11 2029688411025491 7511 TTCCTAGGGAYTTGCTAATGT 1.042 1.058 0.252 1.975 1.891 35 1120308077 — 7512 GCTTATGTGTSTGTGAGTACC 0.075 0.074 1.158 1.317 1.996 3511 20315278 7929060 7513 AAGGGTGACTRTTGTTCTATC 0.000 0.000 1.376 1.5381.856 35 11 20335244 10833310 7514 AAGATCCACTRCTTGCACAAG 2.180 2.2611.539 1.539 2.275 36 11 95101359 1944076 7515 GCAGAAAAGGSTAGAATACAG0.026 0.025 0.063 0.131 0.214 36 11 95109994 12287206 7516TTCTGAGGCTYTTAGCTCATT 0.164 0.161 0.113 0.152 0.153 36 11 951241421255149 7517 CCAAATGATTRCCTTGTTGTT 0.067 0.066 0.091 0.096 0.256 36 1195129000 1569057 7518 CTGGAAGTTGRAGCTTAGAAC 1.042 1.012 0.165 0.1350.266 36 11 95139861 1255167 7519 ATGTATTGTTYAAATTGAGCG 0.056 0.0550.181 0.138 0.303 36 11 95150156 12418346 7520 GAGGAACACTRACAAAAGCAG0.259 0.285 0.533 0.373 0.492 36 11 95153145 1255169 7521TCTTCTTTTGSAGTAACTCAA 0.164 0.169 0.215 0.450 0.402 36 11 9517496616922554 7522 AGAGGAAAAAYGAAATAATAG 1.055 1.091 0.576 0.576 0.489 36 1195188216 1255171 7523 CCTTCTATCTYTTTGAACGCT 0.145 0.133 0.646 0.7310.546 36 11 95195830 12421552 7524 GCAGTCATACRTACACTTGAA 0.995 1.0000.848 0.819 0.621 36 11 95201389 564480 7525 CACCTGACATYACATTCATTT 0.4450.443 1.069 0.930 0.616 36 11 95206876 16922618 7526GGAAAAAAAARAAATGGCAAC 0.000 0.000 1.230 1.026 0.397 36 11 9521383217229289 7527 TCACAATAAASCAGGTGTTTT 1.577 1.561 0.967 0.787 0.630 36 1195221172 12418620 7528 CATATCTGAGRAATAAACTCT 0.228 0.240 0.853 0.7400.780 36 11 95228404 515546 7529 TTTCTTTTAASACTATTAAGT 0.000 0.000 0.8530.372 0.763 36 11 95238277 687559 7530 TAAGATGCTGRGAAGACTGCA 0.275 0.2820.056 0.601 0.461 36 11 95254194 643862 7531 GCTAAAATTTKGCCAAGTTCT 0.0000.000 0.033 0.715 0.413 36 11 95259984 17840711 7532GATATAAGTAWCCTCAACTTA 0.022 0.021 0.231 0.198 0.315 36 11 9526541212422059 7533 CTAGGCCTATRTACTTCATCT 0.121 0.123 0.458 0.143 0.243 36 1195273880 561635 7534 TTATTTCTTCYAGTTGTCCCC 1.030 1.000 0.298 0.089 0.49036 11 95292570 12417555 7535 AGAAGGAATCYTTTTTAATCC 0.808 0.814 0.2930.145 0.179 36 11 95295504 16922701 7536 CTTTAAGCCAMCAATTATAGT 0.0420.043 0.273 0.103 0.168 36 11 95327557 11021358 7537TTTCACATTCWTGGTATTTTC 0.052 0.049 0.156 0.435 0.128 36 11 953343611940208 7538 GGTCAGTTAARTTTGGAGATC 0.002 0.002 0.025 0.420 0.127 36 1195344073 16922748 7539 CATATCTTTAYAGCGTTACTG 0.590 0.582 0.406 0.2170.164 36 11 95354964 16922792 7540 TCAGAAGACAYGGGAAACGCA 0.070 0.0640.404 0.100 0.217 36 11 95362324 7113517 7541 TGAAAATCAAYCTCTGTGTGT1.550 1.574 0.459 0.129 0.224 36 11 95370249 7110427 7542CTGCCAGACCRATGAAAACAT 0.067 0.070 0.289 0.242 0.130 36 11 9538160611021381 7543 CTAAACTAACRGAGTCAACTA 0.136 0.136 0.366 0.326 0.069 36 1195386077 11021388 7544 AACCCTAAGCRTTCTTTGTAT 0.024 0.024 0.112 0.2250.068 36 11 95410392 10831467 7545 AGATTGATACYATGACTTCTG 0.248 0.2530.154 0.280 0.059 36 11 95417356 17233750 7546 CCCTAGGAGAMACCTTAATCT0.709 0.755 0.135 0.043 0.100 36 11 95423237 7125575 7547AAGAATGAATSCTTCACAGGG 0.337 0.332 0.220 0.034 0.063 36 11 954296071940153 7548 GTATGTGTAGRCTACCATTCA 0.139 0.137 0.186 0.024 0.085 36 1195435937 10831474 7549 ACATTCCTGCYGTCTCACTTC 0.261 0.284 0.040 0.0670.011 36 11 95448039 1939485 7550 ATTATTGATAYTGTGCTATCC 0.128 0.1300.010 0.057 0.011 36 11 95453741 11021425 7551 CATAATGCATRGGTTTGCCAG0.018 0.018 0.042 0.020 0.041 36 11 95459675 10831478 7552TGACTAAAGAWCCCTCCCTAC 0.022 0.022 0.025 0.017 0.049 36 11 954673876483478 7553 TCATTCACAAMCTAGCAATCA 0.482 0.489 0.044 0.017 0.043 36 1195474499 6483479 7554 AGCAGAGCTCYATCACCGGCA 0.153 0.158 0.081 0.0680.523 36 11 95483812 4753725 7555 TGCCTATCATRTATTATCAAG 0.233 0.2370.098 0.061 0.468 36 11 95493742 10765791 7556 GCCTCCAAATYGCTCAAGGGA0.217 0.225 0.213 0.069 0.481 36 11 95500304 7951852 7557TTCCATCAGCRCCAAATAATT 0.162 0.168 0.187 1.162 0.467 36 11 9551134217236265 7558 AATGTTGAACYCCTAATGGCT 0.897 0.899 0.156 1.025 0.474 36 1195520632 1939472 7559 GGGTATTGTTYGGAGCAAAGT 0.055 0.055 1.544 1.0160.753 36 11 95539369 12575359 7560 GTTTCCTAACYTGCTGCCACC 0.088 0.0941.759 1.008 1.018 36 11 95541663 2155001 7561 GTGCTGGCTGSTGTGGGGATT4.036 3.386 1.588 0.988 0.986 36 11 95554246 7117004 7562TTAAAATCTCYGCCTTTCTTT 0.086 0.065 1.511 1.361 1.273 36 11 955664064753743 7563 TGTGATTCAARAAGACAAATT 0.192 0.183 1.613 1.426 1.201 36 1195572963 553652 7564 ACCTTAGTCCRAACAGATGAA 0.246 0.248 0.266 1.468 1.13936 11 95579484 470141 7565 TTTGTGCTTTYCAGACCTAAA 0.183 0.185 0.635 1.8631.296 36 11 95587058 10831497 7566 GGTCTTTTGARGCCATGCCTA 1.211 1.1940.727 0.593 1.117 36 11 95597365 12577742 7567 TAACTTTCTTYTCCTTGTGCC0.993 0.965 1.190 0.582 1.134 36 11 95605131 7109768 7568GAGAAATTGTYTGGCATAAAC 0.400 0.404 1.254 0.696 1.170 36 11 9561145010501848 7569 ACTTCCTTTAYTGATATGATA 1.222 1.375 0.636 0.639 0.334 36 1195617867 485642 7570 AAAAAAGCCARTGCAACTGAT 0.146 0.154 0.431 0.618 0.39236 11 95624336 4278478 7571 CTGCCTGTATYGTTATTAGTA 0.013 0.013 0.3000.277 0.386 36 11 95630786 10831503 7572 AGATTTGCTTMCTTGATCCTA 0.5910.563 0.031 0.248 0.770 36 11 95639748 7115578 7573ACGTTATTGARAAGTAAATCT 0.046 0.048 0.028 0.227 0.795 36 11 95648764557137 7574 CATTCCTTCAKCAAGAGGGTT 0.127 0.125 0.218 0.056 0.482 36 1195655702 537756 7575 CCTTAATGTAYACACTTGCAA 0.144 0.135 0.141 0.390 0.28636 11 95662862 485842 7576 CTTTGTTAGCYGTTGGCAGAG 0.882 0.861 0.157 0.4750.263 36 11 95674082 11021527 7577 TGCATTTTAGYCCAGTTTTTC 0.302 0.2960.892 0.310 0.131 36 11 95682189 477556 7578 CAATTATGAGYAATTCATTCT 0.2110.180 0.939 0.326 0.104 36 11 95688991 494901 7579 GCTGTTCCTTYATACCCTATC1.778 1.928 0.538 0.372 0.160 36 11 95696359 542284 7580AAGGAACTTAYCTGTCTTCAA 0.288 0.281 0.477 0.437 0.098 36 11 956978934753195 7581 GTCTGACATCYTCATTCCATT 0.052 0.048 0.504 0.196 0.270 36 1195711821 10501852 7582 CGTGCTGAGTRACAACGGGCC 0.135 0.137 0.047 0.2310.278 36 11 95717420 12791333 7583 AATACTGCAAYAGTCAGGACC 0.221 0.2220.009 0.195 0.731 36 11 95727253 10831513 7584 ACTGGTTAAARAATTGAACAA0.339 0.339 0.028 0.090 0.522 36 11 95734731 4753748 7585TCACAAAGCARATAAAGAAGA 0.032 0.005 0.024 0.072 0.613 36 11 957477096483501 7586 TGTAATTTATYTACATTGCCA 0.000 0.000 0.333 0.597 0.692 36 1195759253 12273522 7587 AAATTATAGCRTGTAGGATGA 0.093 0.091 0.249 0.5580.355 36 11 95767033 10765805 7588 GGTTCTGTATYTGCCCCCAAA 1.219 1.1741.152 0.708 0.350 36 11 95777788 — 7589 AATCTACTTTKTCACTAATAG 0.1330.133 0.914 0.749 0.358 36 11 95798914 10501853 7590TCTGACTGACRTAATGGATCA 2.091 1.972 1.124 0.766 0.505 36 11 958109951451333 7591 GAAATTTACCRAAAGTACAGA 0.111 0.108 0.899 0.666 0.593 36 1195816568 7935312 7592 GATTTTTAGASCTCTCTTTGT 0.724 0.717 0.886 0.6640.565 36 11 95823847 7928913 7593 GCACTGATAAWATTTCCTGCA 0.491 0.5080.281 0.635 0.607 36 11 95837900 7935320 7594 GATTGTGACGWAAAGTCCTTG0.217 0.212 0.269 0.766 0.578 36 11 95842433 7114658 7595CCTTGGGAATKATTTCGCTTG 0.218 0.212 0.331 0.315 0.585 36 11 9585456616923596 7596 CTCCTTGAGCRCTCCCTGATT 0.061 0.059 0.363 0.366 0.510 36 1195868655 618929 7597 GTTTCTCAGARTGTGTCTCCA 0.960 0.942 0.347 0.272 0.60236 11 95882778 564259 7598 AAAACCCTGCRGGGAAAAGAA 0.703 0.687 0.412 0.1650.257 36 11 95899268 10831534 7599 CCTGTACTTGYGAAACACTTG 0.191 0.1880.518 0.281 0.352 36 11 95908847 1451329 7600 AATATACAGGSAATTATAGTA0.322 0.322 0.187 0.391 0.285 37 12 56066088 4108243 7601TTACAAAAATRAAAAAAATGC 0.117 0.117 0.367 0.342 0.640 37 12 56096521537482 7602 GGCTGCCCAAYAGAAACTTGC 0.948 0.966 0.334 0.429 0.598 37 1256102189 1798710 7603 ATCTATCCCTSTGCACTTGCA 0.129 0.136 0.211 0.6050.742 37 12 56112400 481196 7604 TCAACCCTCTRGAGAACAACA 0.001 0.001 0.5800.670 1.176 37 12 56145698 7305145 7605 AGTGCCGGGARGAACCCTTGG 0.3630.352 0.691 0.504 1.195 37 12 56152088 2228226 7606TGGGGAAACASAATTCCTCAA 1.157 1.228 0.807 0.772 1.458 37 12 56163423511752 7607 CTGGATGATAYAATTTCCTAA 1.294 1.274 0.794 1.437 1.752 37 1256177450 17119735 7608 CAGTGACTGTRGGCTAAGAAT 0.328 0.325 1.101 1.7101.901 37 12 56197735 703835 7609 AAATTTTTGGRAAAGGGTAGG 0.057 0.040 1.8611.944 1.901 37 12 56224348 775238 7610 CCTACCTACAYCCTCCTGTAT 0.984 0.9941.792 2.104 2.040 37 12 56232777 11172247 7611 ACCAGGTCGGSAGCTGGCTTA2.851 2.568 1.960 2.032 2.065 37 12 56244580 775322 7612GTCCCAGTGGRTGAGGGTGTG 1.036 1.041 2.227 1.869 2.377 37 12 562549821678542 7613 TGAAATGCCGSTGATAGAGAG 2.026 1.950 2.025 2.100 2.467 37 1256262914 2888334 7614 GTGATAAGGTSTGGACGAAAA 1.210 1.247 1.541 2.1812.735 37 12 56265007 775251 7615 GAACCCAGAGYTGATTTAAAA 1.336 1.355 1.9342.362 2.687 37 12 56288866 2306390 7616 CAACCCCTCGYTCACGGAGCC 0.6570.669 1.711 2.214 2.713 37 12 56299442 2277324 7617TCCAAGCCGCRGCTCTCGCCA 1.580 1.592 2.470 2.845 2.778 37 12 5630720010083154 7618 ATATACTTGTRCTTTGAAAAT 1.432 1.412 2.789 2.813 2.687 37 1256351715 2640629 7619 GTGTAATAAAYAAAAGGCAAC 3.294 3.322 4.665 3.1532.443 37 12 56376989 7309600 7620 GCATCCCTGCRAACAGCTTCT 0.774 0.7323.474 3.332 2.433 37 12 56393103 1689590 7621 AGGGCAATATRTAGGTTTGTT2.832 2.761 3.687 3.534 2.381 37 12 56404515 2269720 7622CTGCAGCTCAYGGAGGCAGCA 1.263 1.282 2.474 2.801 2.355 37 12 56415927238527 7623 CATTCCACAGKAGAGAAACTG 0.546 0.528 2.012 2.408 2.345 37 1256444371 8176346 7624 ATCCACTAGTYGCTTCCCCAG 0.251 0.244 0.687 1.6382.258 37 12 56451352 10877013 7625 TTGGTCACACYATTGCAGAAG 0.128 0.1200.366 1.364 1.941 37 12 56459196 10877019 7626 GCTGCCAAGAYTTTGTCCTCT0.761 0.785 0.365 0.548 1.685 37 12 56466683 724834 7627TTTTAGAGGASGTTTGGGGGC 0.503 0.524 0.287 0.357 1.129 37 12 564744117976556 7628 CTACCCCTTTYTACCTTTTGG 0.518 0.523 0.455 0.381 1.079 37 1256483069 1875124 7629 GATTTTTCCARAGCAAGGTGG 0.007 0.007 0.393 0.3610.510 37 12 56488686 2277326 7630 TATGAAGCCAMATGATATAAG 0.557 0.5400.411 0.462 0.308 37 12 56494431 1021469 7631 CAGCCACAGGRTTAACGTGGC0.613 0.642 0.276 0.360 0.330 37 12 56508939 4760332 7632AAATGCCTTGMATGAGTATGG 0.568 0.579 0.460 0.308 0.525 37 12 565148557489290 7633 AGGCCCACAGSGATTTCCTTC 0.098 0.100 0.439 0.253 0.617 37 1256521865 4760334 7634 TATGTAAAGARGGAGGACATT 0.517 0.500 0.321 0.4461.029 37 12 56548790 7312623 7635 GAGAGAAGGGKTCAGGGGCCC 0.000 0.0000.195 0.648 0.934 37 12 56566859 11172369 7636 ACTGAGGGTTYTCAAGGCCAC0.329 0.340 0.408 0.588 0.924 37 12 56573897 7971877 7637TGGGAGGGAAKAATGTCCCTG 0.362 0.327 0.717 1.313 1.260 37 12 565885254630335 7638 AGTAATTGAARGCCTTAGGTT 0.647 0.633 0.717 1.339 1.216 37 1256589754 — 7639 AATTTCCTTTRTAAAGAAGAC 1.188 1.117 1.843 1.242 1.123 3712 56612866 17120198 7640 ATAATGCTTAYTCTTGATGTT 0.000 0.000 1.700 1.4901.047 37 12 56621010 4760346 7641 AGTAGGGTAARCGAATGGGTA 2.339 2.1461.625 1.512 1.176 37 12 56627592 7132343 7642 TAGGATTGGARGGAAATGTAA0.042 0.043 1.690 1.435 1.184 37 12 56635863 17120058 7643ATGTTAGTGARTATCTCCAGC 0.585 0.568 1.482 1.288 1.181 38 12 813459811874058 7644 GCTCCTTGATKACCATTTTTT 0.000 0.000 1.164 0.588 0.322 38 1281358422 2731295 7645 TGTACCAGGCRATGCTTCCAT 1.505 1.445 1.014 0.5880.473 38 12 81366314 1494024 7646 CCATCAAAGASAATAATCACT 0.131 0.1290.412 0.528 0.488 38 12 81372465 17009623 7647 AGAAGAAACTRCCTTAAGTAT0.186 0.186 0.412 0.534 0.798 38 12 81377879 7133956 7648TTAAATGTGGRATGCAAGCAT 0.047 0.048 0.006 0.527 0.811 38 12 813825787967097 7649 AATTGCAGTAYTACTTTTGTC 0.000 0.000 0.054 0.460 1.001 38 1281413513 17776375 7650 CTTGTTGGTCYCATTGCACTG 0.026 0.025 0.411 0.3741.029 38 12 81423611 1565801 7651 CATTATCTGAYAACCAGTAGA 0.516 0.4890.502 0.520 0.742 38 12 81429920 10506875 7652 TGAACATAGTRTCTAGATCTA1.272 1.189 0.746 0.732 0.683 38 12 81434488 4882510 7653CACATTGGTCYCAAATTTCAA 0.241 0.254 0.936 0.861 0.441 38 12 8145662012580555 7654 GGAATACAGTRTGCCCTTTCA 1.192 1.118 1.125 0.766 0.481 38 1281462973 11834171 7655 ACCCAAGAGTKAGGACTAGTA 0.615 0.609 0.967 0.8330.562 38 12 81469479 17720596 7656 ACTGTTGTTCMTCATAGTATC 0.992 0.9300.885 0.828 0.712 38 12 81474730 12582370 7657 AAAGCAAATTKGTTTTTTCCA0.480 0.537 0.532 0.604 0.661 38 12 81489232 17777291 7658ATAAGGACCAKAAAGGAATGA 0.204 0.200 0.449 0.687 0.663 38 12 8149663917009739 7659 TGAATACATCYGAAGTAAAGA 0.267 0.269 0.238 0.585 0.622 38 1281502516 11115314 7660 ATTCCAGAACKTTTGTCCTTA 0.425 0.419 0.266 0.4990.580 38 12 81508836 12821789 7661 AGAGATTTGAYATACATTCAA 0.316 0.3200.483 0.275 0.683 38 12 81521180 17009794 7662 GAGTAGCTCTWCATTTGCCAA0.593 0.604 0.493 0.209 0.579 38 12 81548422 10506877 7663GATAAAAGTTMCTACTGAGGA 0.795 0.816 0.341 0.372 0.504 38 12 8156176117722367 7664 ATCTGACTTCYAGCATTCAGT 0.360 0.315 0.278 0.494 0.330 38 1281570312 7316112 7665 GTATTCCACARAAGAATAAAG 0.001 0.001 0.414 0.5610.245 38 12 81579199 11115342 7666 AGATGTGGATYTTTAGTTAAT 0.190 0.1900.390 0.535 0.263 38 12 81592425 10862496 7667 TCAAATGATGMAGAAGCTTAA0.976 0.954 0.536 0.398 0.341 38 12 81602869 11115353 7668AGGATTTGTAKTGCCAGTGGT 0.761 0.797 0.640 0.204 0.342 38 12 816108634620789 7669 AGGATAATGTKCTAGATAGCT 0.677 0.683 0.607 0.204 0.284 38 1281617342 17009899 7670 TTACATTTAAYGACAGGTTTT 0.000 0.000 0.249 0.3870.348 38 12 81627538 10506879 7671 CAAATGTTTCYGCGTAGGTTG 0.127 0.1200.125 0.460 0.232 38 12 81642625 7972064 7672 TTGGCACTGTSCATGTTTTTG0.069 0.069 0.141 0.211 0.255 38 12 81659668 10506880 7673TGCTATCCAGRACTGTCCACT 0.311 0.313 0.174 0.250 0.304 38 12 816683237312288 7674 GCACCATGGCRCTTAACTGTA 0.736 0.719 0.154 0.151 0.325 38 1281678985 6539689 7675 ATTTTCTACCYTTTCTAGACT 0.000 0.000 0.499 0.2040.170 38 12 81685472 12368738 7676 GTGAATTGCASGTGTAGGAGG 0.052 0.0520.435 0.236 0.111 38 12 81691248 7309393 7677 GTGAATCGCTKGAATCCGAAG0.900 0.880 0.340 0.289 0.107 38 12 81692856 17009995 7678TTAGGGGAAARGTATATATAT 0.192 0.194 0.269 0.223 0.082 38 12 817106131858059 7679 CAAAGCTCCAWGGGAGAATAC 0.414 0.416 0.326 0.143 0.080 38 1281719954 17041817 7680 TTATTTTTACRGAAAAACCCC 0.291 0.279 0.081 0.1800.111 38 12 81729819 17010029 7681 TCACAAAATGYGTAGTATATT 0.338 0.3280.118 0.156 0.125 38 12 81741749 9651971 7682 AAGCCTTACARTATCCCTTCT0.063 0.063 0.158 0.041 0.081 38 12 81752531 12300038 7683TCTATAGAGGRTAGTAAGTCC 0.326 0.328 0.098 0.058 0.063 38 12 8176668617010069 7684 TACTGTTTGAMATAGTTTATC 0.572 0.541 0.050 0.058 0.061 38 1281776529 1703109 7685 TAAATAAAGCSTCTAGTACCT 0.005 0.005 0.095 0.0660.038 38 12 81792738 17010106 7686 AGAGCCCTTTMTGTCAAAGTC 0.151 0.1450.128 0.047 0.130 38 12 81801101 17726837 7687 GGCACCATTGRTCTCTGTTAC0.357 0.359 0.086 0.047 0.146 38 12 81808589 9308329 7688GTCGTTTAATRTTTGGGGCTC 0.429 0.424 0.119 0.091 0.305 38 12 8181934417010128 7689 ATGTCTGCCCYGGGCCACACT 0.369 0.348 0.109 0.213 0.418 38 1281829259 10778916 7690 AATTCCTAGCRAAAAATCTGT 0.150 0.155 0.192 0.3500.498 38 12 81840419 — 7691 TGGTGTCATARTTATGTAAAT 0.214 0.089 0.4950.681 0.528 38 12 81849545 12297736 7692 GCTAGTGCATRTTAGTTACTT 0.6720.657 0.597 0.846 0.450 38 12 81857607 17010268 7693GCCCAAATTTWTCCAGAACTT 1.150 1.142 1.034 0.811 0.529 38 12 818694992403024 7694 CCATATAACTRGATTAGTCAT 0.535 0.534 1.318 0.864 0.736 38 1281877247 10778922 7695 TTTCTTTGTCKCTAGATTGTA 1.114 1.101 1.154 0.8930.678 38 12 81887096 10778923 7696 TAATCCCTTCMTTTGGTCAAC 0.859 0.8230.942 0.950 0.774 38 12 81901994 1655593 7697 CTCCCAGGCCRCTAGCAAAGC0.329 0.347 0.782 1.052 0.721 38 12 81912723 1682605 7698GTAACAAGAGYTCTTTTAAGT 0.000 0.000 0.374 0.715 0.728 38 12 8191918110862550 7699 ATTGAAATGAYTTTTGGCTAC 0.240 0.243 0.416 0.807 0.962 38 1281925847 11115552 7700 TTGTCTTTACMATGGTCTGTG 0.324 0.332 0.357 0.5080.825 38 12 81931444 12301199 7701 TTAATTTATAYTTGCAGCAAT 0.901 0.9620.539 0.297 0.735 38 12 81937271 12315833 7702 AAGAAGGGAASACTGGCCTTT0.193 0.198 0.502 0.578 1.112 38 12 81946596 12814295 7703TTGAGGTACAKTTGAAAGAGT 0.853 0.853 0.434 0.427 0.829 38 12 8195343112833644 7704 ATATAATCTTYCTAGATATGG 0.182 0.181 0.565 0.542 2.029 38 1281961580 10778937 7705 ATTTTATATAYGAAGAATTCC 0.132 0.137 0.472 1.0742.019 38 12 81987033 10862580 7706 ATCACTCTCCYGAATTAAGTT 1.268 1.2780.374 0.767 1.909 38 12 81996914 10506890 7707 TCTGATCAACRTAAAATTAGG0.005 0.005 1.281 2.771 1.958 38 12 82007514 11115596 7708CCCATTTAATKTTTAAACTAG 0.658 0.636 1.276 2.679 1.950 38 12 820195331543171 7709 TGTCATCTGTKTCTGATTTGT 1.822 1.835 3.156 2.661 2.563 38 1282029933 2292827 7710 CTATAAATTARGTAGAGAACA 0.087 0.086 3.869 3.0883.099 38 12 82038186 12146710 7711 TTAGGGGTTTMAATTTCTTCT 4.300 2.0673.117 2.572 3.337 38 12 82046326 11115618 7712 AAAAATAACAWGTTGAAGTAA0.562 0.562 2.139 3.481 3.130 38 12 82054366 17010700 7713GCCACCTCACRCAGAATTGTG 0.160 0.168 2.283 3.298 2.824 38 12 8206207117010710 7714 CCTGCTTACCMAAAACTGCAC 0.301 0.296 1.297 3.229 2.362 38 1282075135 12301625 7715 ACAATGTGCCYGTTCAGATAT 0.364 0.388 1.417 2.9552.519 38 12 82101670 7296128 7716 TGCTGTGAATRTTCTATGAGC 2.323 2.2561.880 1.132 2.370 38 12 82106660 2037258 7717 CTGTGCCAGGMAGTTTGGTAA0.710 0.690 1.842 0.854 1.918 38 12 82110836 2037257 7718AGTTTCCTTAYATATAGCAAA 0.881 0.851 1.396 1.046 1.813 38 12 821328866539713 7719 ATTAAATTTGYTATTACTGCT 0.480 0.484 0.437 1.070 0.712 38 1282145316 7311826 7720 TTTGGAAAATKTGCAGGCCTA 0.209 0.211 0.459 1.1300.595 38 12 82157291 7296363 7721 AAGTTGGTAAMAAAAAAACAA 0.044 0.0420.299 0.488 0.652 38 12 82166950 6539716 7722 TTTTGTCTTTKTATGGTTTCT0.773 0.770 0.422 0.326 0.618 38 12 82180501 6539722 7723AAAAGATACCYAAAAGTGCAG 0.397 0.393 0.330 0.164 0.591 38 12 821951474882445 7724 GCCTTCAGAGKCATGAATGAG 0.869 0.854 0.329 0.157 0.201 38 1282206913 6419410 7725 TTATTCAGTCWTTGACAGTGT 0.006 0.006 0.122 0.1340.211 38 12 82224121 7485518 7726 GAACAAGAACMCTTCAATAAA 0.067 0.0680.141 0.159 0.113 38 12 82233165 4882378 7727 TTAATTTCTTKAGGAGAAGCA0.051 0.054 0.016 0.064 0.249 38 12 82249263 6539740 7728CAGAATTAATKTCCTTGTGCC 0.471 0.460 0.043 0.105 0.754 38 12 822639264471519 7729 CAGAAAATTARACTCATTGGA 0.181 0.179 0.053 0.024 0.909 38 1282268637 4346039 7730 ATTTATGTTCRGAAATAACAT 0.251 0.242 0.228 0.2720.749 39 14 49608345 2103889 7731 TGCTGCTGTAYACTTTTTCAT 0.722 0.7570.500 0.542 0.657 39 14 49617560 17122058 7732 ACTTAACCTTRGCATAGTAAT0.193 0.187 0.372 1.211 0.636 39 14 49630695 7141809 7733GCTGCCTTTARGATCAACCTA 0.034 0.034 0.140 0.730 0.692 39 14 496381769944008 7734 AGCTTCGTATRAAAAAAAACA 0.217 0.193 0.618 0.598 0.584 39 1449645558 10844 7735 ATCACATAGAYATGTATGGTG 0.000 0.000 0.572 0.319 0.71139 14 49654998 2227276 7736 AGCATCGACGYGGTACCCTTG 1.894 1.887 0.6130.253 0.436 39 14 49666612 3759597 7737 AAAAATTAACYCAGAACCTTC 0.0550.057 0.565 0.309 0.465 39 14 49673621 12896468 7738AACATTCATTYAGCATAAACA 0.095 0.096 0.541 0.479 0.314 39 14 496866288192699 7739 GGCTTACTCCRTTTGCCTGAG 0.081 0.081 0.039 0.473 0.322 39 1449692566 13379306 7740 CAACAGTAATMAAGGAAACTT 0.452 0.444 0.217 0.5160.462 39 14 49703571 17122240 7741 ATTTCTTTACRTGCTTTTTTA 0.000 0.0000.272 0.115 0.500 39 14 49720888 6572646 7742 CTAAGAATATYCTGAGTGATA0.618 0.621 0.432 0.267 0.520 39 14 49728687 7160215 7743AGTAATACTAMCATGAAAGAG 0.108 0.105 0.307 0.478 0.709 39 14 4973697712896404 7744 TGGCCTACTAYATAGGAATTA 0.643 0.630 0.412 0.524 0.604 39 1449740883 12888783 7745 TGAACCTTTARAAAAAAGTTA 0.082 0.083 0.549 0.4630.854 39 14 49752478 1569898 7746 CTTCATTGTGYTGCCAAGATT 0.783 0.7600.633 0.742 1.038 39 14 49764449 2144575 7747 TTAATTTCTAYTGACTATTTT1.055 1.009 0.477 0.987 1.275 39 14 49769618 1955926 7748AACTACTGTGMTGTCAAACTA 0.315 0.296 1.134 1.410 1.315 39 14 49784735743075 7749 TGTGAAAAATYCTATCTTATA 0.263 0.258 1.315 1.520 2.306 39 1449802697 2355885 7750 TCTATAAAGCYTGCCAACATC 1.502 1.517 1.285 1.6132.411 39 14 49806475 2153553 7751 TGAGCAATTAYCGTGTTACTC 1.374 1.4291.522 1.568 2.714 39 14 49820327 12433038 7752 AGAAAAGACARGGATGGCTTA1.418 1.387 1.873 2.456 2.579 39 14 49826881 9888567 7753ATTTGATTAAMTTTCCTAAAT 0.906 0.912 1.796 2.630 2.556 39 14 498394672297995 7754 CGCCACCAACRATGACTATAT 1.073 1.073 3.496 3.191 2.693 39 1449843620 7143204 7755 ACAAAAGCTTKTGGGGAGGTA 0.000 0.000 3.551 3.2842.548 39 14 49857963 2275592 7756 CAAATGATGCYGTTTGGAAAA 4.633 5.2673.518 3.026 2.548 39 14 49868493 4901016 7757 CATGCAGGCTRGAAACCCTGG2.056 2.080 3.924 2.920 2.777 39 14 49880775 1465160 7758CTGCAACTGAYATACTGCAGC 0.954 0.980 3.187 2.791 2.642 39 14 498891192153552 7759 GAAGAATAGARTTGAGGGGAA 0.086 0.085 1.486 2.756 2.661 39 1449894589 11157737 7760 TCTTCTTCCARTGCACACAAA 0.338 0.338 1.176 2.6522.358 39 14 49904329 9635166 7761 GACTCAGATTKACTTTCTCTT 1.348 1.4140.824 1.196 2.294 39 14 49914981 7151046 7762 AGGACACCTGMAGACAGTTCT1.413 1.538 0.994 0.736 2.317 39 14 49930907 11157741 7763CCCACCCATTYCAATTTATCC 0.034 0.035 0.843 0.518 2.280 39 14 4993978317717642 7764 GAAAAGCCCTRCAAAGTGTAC 0.398 0.364 0.457 0.558 1.851 39 1449950187 1265880 7765 CCAGCTACTTYTGCCTCCTTC 0.074 0.075 0.082 0.6061.257 39 14 49964206 12887920 7766 GAGCATGCAARCAATAATAAA 0.470 0.5140.106 0.283 1.004 39 14 49976878 17718015 7767 GTGTGTAAATRCCATCTTCAG0.270 0.260 0.088 0.888 1.281 39 14 49986853 17122546 7768ACCGTAAGTCKGTGATTCTTA 0.191 0.131 0.205 0.931 2.073 39 14 49999380 —7769 ACAAATAAACYGTGTTAGTAA 0.000 0.000 1.744 0.836 1.555 39 14 5000964917791470 7770 ATGTAGTTTTYTAATTGGTCT 0.386 0.400 1.598 1.147 1.192 39 1450011797 12717408 7771 AAAAAATTTAYTGGCACTTTA 3.021 2.690 1.429 1.7201.213 39 14 50028605 12323534 7772 AATTATTAACRTGATGCTTCG 0.201 0.1931.469 1.694 1.158 39 14 50035195 17791680 7773 TAAGTTTACARCTGGAATTTC0.080 0.081 2.173 1.734 1.093 39 14 50046648 2180494 7774TTCTCGGTTAMCAATTATTAA 0.760 0.755 0.972 1.591 1.016 39 14 500626867160618 7775 TCATCCCCAAYAAAGGCAGAG 1.918 2.006 1.162 1.737 1.013 39 1450074963 17718908 7776 TTGTCTGCAARTTCAATAGCT 0.516 0.533 1.214 0.5900.963 39 14 50093893 987315 7777 CATATCCTTTYCTTTTTCCAG 0.554 0.546 1.0520.581 0.923 39 14 50139753 3015452 7778 TTTTCACCTAYAGCATGTTTA 0.3560.343 0.252 0.650 0.929 39 14 50153985 17122693 7779CAATCACTTAYCAGAATCAGG 0.299 0.303 0.136 0.468 0.391 39 14 5016415117122701 7780 TTTGGATATAYGTAGTTTTCT 0.044 0.046 0.078 0.161 0.419 39 1450174491 17122714 7781 ATCAAGAGTAYGATTATGGGT 0.153 0.147 0.052 0.1520.439 40 14 80247029 17111027 7782 AAAAATTATCRTCTGAAATCA 0.012 0.0120.303 0.298 0.268 40 14 80254648 17111035 7783 CAGTGAGAATYTGGAACACAG0.140 0.142 0.286 0.567 0.230 40 14 80273661 327456 7784TTGAACTTCTRAACAAGCTGG 0.302 0.300 0.128 0.441 0.327 40 14 80288710327446 7785 GGGCCTAATAYTTCTCACACA 0.095 0.094 0.491 0.468 0.326 40 1480294079 2590480 7786 CACAGTATTTYCTTCCTCTTC 0.861 0.854 0.521 0.1950.346 40 14 80299870 17541411 7787 GATTTAAACAYTTTTTGAAGA 1.062 1.1030.495 0.341 0.308 40 14 80307745 327473 7788 ATAGCCTTAGMTGTCTCATAA 0.1750.177 0.555 0.386 0.400 40 14 80316672 17613911 7789CAGAAGATCCRCAGAATGCTT 0.229 0.225 0.443 0.330 0.238 40 14 8032514717614081 7790 TGATATTTCTRTGGAAGTTTT 0.270 0.263 0.163 0.415 0.352 40 1480331771 327428 7791 AAGGGCCATTRTGACAAAAAG 0.583 0.545 0.141 0.371 0.45540 14 80353610 2371417 7792 ATCTAATAGCRATATCAGTAA 0.350 0.320 0.2080.215 0.633 40 14 80365281 17111151 7793 GAAAATCAGAMGTGTCACTAA 0.1300.133 0.366 0.314 0.629 40 14 80376971 7142753 7794TGGATATGAGKTACACAAAAT 0.425 0.422 0.312 0.439 0.474 40 14 8038190511159475 7795 TTACTATGCAYTCAGTAGTTG 0.715 0.708 0.438 0.756 0.290 40 1480393265 — 7796 TAAATTTTTAYCTAGGATTTT 0.463 0.449 0.718 0.578 0.279 4014 80415919 2556611 7797 CCAGATCTACRAAGCTCAAAT 0.646 0.637 1.089 0.5090.265 40 14 80431484 8010968 7798 TAAAAACTACMGAAAAATCAG 0.713 0.7160.757 0.504 0.234 40 14 80444575 6574608 7799 TCTATTCATTMAAGGTCAGTT1.274 1.204 0.601 0.433 0.185 40 14 80452982 4899780 7800CTCACTTAATMCTACCAACAA 0.055 0.054 0.384 0.287 0.205 40 14 80455324 —7801 GAAGACAACTYCACCAGATAT 0.095 0.095 0.200 0.215 0.248 40 14 804605442059719 7802 AATGAAAGATSAAAGTAAATA 0.140 0.138 0.010 0.127 0.202 40 1480470519 9323695 7803 GCACATAGCTRGAAATCAGAA 0.213 0.211 0.011 0.0870.206 40 14 80478424 17111256 7804 AAGTAGATGTRAGGCTGTCAG 0.179 0.1730.021 0.019 0.206 40 14 80484907 2217177 7805 ATTTGCCCCAYTTTAAAAGTT0.081 0.084 0.052 0.019 0.167 40 14 80492010 2239610 7806GAGGTGGCCCSAAGTGCACAA 0.197 0.205 0.102 0.071 0.155 40 14 80499216179245 7807 GATATCCCAASTAGGTCAACA 0.384 0.395 0.093 0.113 0.063 40 1480511106 179259 7808 AATCTTAGCARGGCACATAAT 0.448 0.462 0.264 0.149 0.07740 14 80519443 3783945 7809 AGAGCTTTTTSATTTGCCATC 0.037 0.038 0.3230.231 0.152 40 14 80527693 724170 7810 ATTCAACATTRAAGGAGACAT 0.758 0.7500.315 0.297 0.193 40 14 80540892 17545310 7811 ACTGGAAGTCMTATTCATGGT0.394 0.390 0.366 0.287 0.181 40 14 80547190 4903965 7812AGGAACTAACRTGACTGAGTT 0.358 0.371 0.500 0.409 0.173 40 14 805549024903967 7813 TGAAAAGTTGRTCACCCTATG 0.597 0.626 0.304 0.386 0.252 40 1480566095 10143800 7814 AGTTCTGCACRAAGGCCTCTT 0.396 0.391 0.481 0.4020.291 40 14 80570637 726627 7815 AATTTAAGCAKAAAAAGGGTC 0.230 0.231 0.5000.237 0.366 40 14 80585638 722540 7816 TCCTCAAAGCYCAGATGGTTC 0.835 0.8600.288 0.293 0.568 40 14 80598165 17111401 7817 GCTTTTTAATWATGTGTTTTT0.390 0.392 0.192 0.324 0.648 40 14 80610085 7145101 7818ATATAAACCCYATTGATCTTA 0.113 0.105 0.322 0.362 0.599 40 14 806235392284735 7819 GACAAAATAARCCCAAGTGAA 0.125 0.125 0.202 0.692 1.181 40 1480631310 10143087 7820 TAGCCCAGAGSTCCCAAGGCT 0.612 0.622 0.346 0.7181.200 40 14 80639431 1017141 7821 TAGCAATTTGRTAATTTCTCC 0.496 0.4860.879 0.605 2.021 40 14 80648058 2888049 7822 GAAGTGGTCARTTAAACTACC0.770 0.750 1.011 1.348 2.611 40 14 80661507 7158881 7823GTAAAGATGGYCCCTGTGGAA 1.389 1.411 0.963 1.429 3.031 40 14 8066866517111530 7824 GTTACTTTCAYTAGACACTTA 0.353 0.355 2.032 2.738 2.838 40 1480677583 2300541 7825 TGCCTGTATGKTAGTGTCCAT 0.539 0.550 1.779 3.1952.656 40 14 80690636 28441485 7826 CTGTTAGGGGKAAGTTTAATG 2.450 2.5462.650 3.422 2.735 40 14 80699947 12372876 7827 AATCAAAACAYGGCAAAGGGA0.379 0.363 3.057 3.127 2.781 40 14 80709737 1957546 7828AGTCACTTCAYCAAGACTGTA 2.971 2.672 3.153 2.652 2.710 40 14 807135534899786 7829 TGGCCACCAAKCAAGAGTTTG 2.630 2.619 2.621 2.682 2.725 40 1480727089 7149672 7830 ATGTTACTTCRAGAAGCACTG 2.331 2.193 2.525 2.6822.770 40 14 80733206 759939 7831 TACTCACTTGSCATGTTAAAT 1.012 1.005 2.3722.400 2.602 40 14 80741859 1951614 7832 CTCTGCTAATYCCAGGCGAAA 1.3151.256 2.070 2.430 2.621 40 14 80747849 7148100 7833TTACACTTCTRGGTTCCCAAG 2.122 1.957 1.781 2.403 2.705 40 14 807834397160569 7834 TGGTTGTGTCYACTACAGGCA 1.268 1.315 1.922 2.152 2.532 40 1480789110 8015423 7835 GAGGATGATGYTTATTGTTCC 1.485 1.419 2.287 1.9772.648 40 14 80797947 17111619 7836 ATCTTCACCAMCCTCTTTGAT 1.045 1.0821.944 2.342 2.481 40 14 80807873 7161073 7837 TTGTCATTCAYCTGCCATACA2.430 2.410 1.989 2.335 2.226 40 14 80817987 8010611 7838CATTAAAAAAYATCAAAAGAC 0.178 0.177 2.453 2.291 2.059 40 14 808262322081988 7839 TATTCCATTGYATGGGCACAG 0.779 0.768 2.010 2.120 2.090 40 1480833708 1885604 7840 GCATGCTATAYGATTTAAATT 1.724 1.753 1.141 1.7952.360 40 14 80834880 17111691 7841 TGTTGCTCTCRGAGATTTAAG 0.131 0.1061.305 1.453 1.923 40 14 80848016 4903980 7842 GTGAGCCTTAYGGTTTGGCTG1.240 1.252 1.100 0.700 1.627 40 14 80859232 8013992 7843GACTAGGCCARGAACAGAGGC 0.876 0.857 0.412 1.042 1.211 40 14 808712917140168 7844 GAGTTGGCACYAAAAATGATG 0.010 0.010 0.419 0.806 0.923 40 1480905656 3853417 7845 TGTTTCAACCSTGATGGCCAC 0.052 0.052 0.384 0.3700.483 40 14 80915606 — 7846 TATAAATTTASTTCAGTGTCA 0.163 0.163 0.1760.404 0.615 40 14 80923160 1569012 7847 GGCTAAAGGAYTCAACGTGAA 1.1671.164 0.238 0.170 0.705 40 14 80930316 3844532 7848ATCTGATGTTYAAATATGAAA 0.158 0.155 0.290 0.075 0.509 41 16 464500363903067 7849 CCATTTTCACRTGCCTCTACT 0.233 0.239 0.060 0.358 0.222 41 1646461498 10492847 7850 CATGCTCTTAYTTTTGCAGGT 0.032 0.033 0.034 0.3480.274 41 16 46467047 3852743 7851 TGGAGTCTTCRGCTATCAGAT 0.045 0.0460.564 0.319 0.216 41 16 46473394 16945694 7852 AAATTACTGCRCACTGCTGAT0.355 0.363 0.582 0.317 0.224 41 16 46479558 — 7853GTGGGGTAAARAATGGAATCA 1.954 1.886 0.571 0.443 0.222 41 16 46498606888339 7854 CCGGTGAGCGYGGTGCTGAGA 0.354 0.326 0.800 0.386 0.199 41 1646515180 — 7855 GGGAAACGCTSATGTGTATGG 0.022 0.022 0.978 0.462 0.148 4116 46517669 8054264 7856 TGAGCTGCAARTGTGGAGACC 0.581 0.575 0.161 0.5490.151 41 16 46532044 9933191 7857 CTGGGAAPAGSTGGGAGACCA 0.565 0.6260.166 0.447 0.198 41 16 46539255 7195967 7858 GAAAATGATGRCTGTTTTCAT0.065 0.065 0.277 0.063 0.246 41 16 46563842 — 7859AGTAGATGACRATGATGACGA 0.334 0.339 0.105 0.051 0.236 41 16 46575693 —7860 CAGGGTCTGTSTTGATGGAGA 0.325 0.332 0.038 0.114 0.187 41 16 4658983012149765 7861 TTCCAGACCCRGGGTCCTACC 0.005 0.005 0.051 0.094 0.023 41 1646626615 494116 7862 GGACATCAAGYTCCAAACCCT 0.223 0.223 0.082 0.034 0.04441 16 46636635 520151 7863 GAATGTGGATYATTTGTTCCC 0.156 0.159 0.104 0.0300.776 41 16 46654645 — 7864 CTGGGAATAAYCTAGTTCCTC 0.572 0.586 0.1060.020 0.913 41 16 46668184 8048367 7865 CAGCTCTGGGRTAAAAGTCCA 0.4000.396 0.068 0.035 0.879 41 16 46679401 16945787 7866TCTGAGGTCTKCCAAGCTGAG 0.017 0.017 0.076 1.240 1.010 41 16 46697131 —7867 AGGAGTAACCYTCAGGCAACC 0.033 0.034 0.087 1.605 1.159 41 16 4670963516945828 7868 GAGGAACCTAKGAACGTCCGT 0.200 0.200 1.544 1.725 1.233 41 1646716800 1345425 7869 TGTGAAGGTCYTTATGGAGGT 0.572 0.552 2.389 1.6381.766 41 16 46722278 12149826 7870 AAAACTTCATYACATTAAACA 4.291 3.5682.798 1.601 1.661 41 16 46729214 16945857 7871 CAAGGAGTGTYGAGTCAGTGG1.133 1.095 3.723 1.782 1.729 41 16 46736483 16945892 7872CACATTGCCTYTCCACAAAGC 0.466 0.477 3.275 2.496 1.577 41 16 467487468058886 7873 CAAGTTTATGRAGGAAGAATG 0.503 0.511 0.895 2.636 1.510 41 1646754604 8050306 7874 GTTTCTCTTAYGATATTGTCC 0.793 0.798 1.225 2.7721.793 41 16 46760004 17821664 7875 CTACGTAGGAKAAGGTTTGCC 0.471 0.4741.016 1.066 1.872 41 16 46768033 10163462 7876 AGGTATCCAARGCCCAGTACC1.875 1.814 1.057 0.736 2.189 41 16 46775302 16945919 7877TCAAACCCCCMGGCTAAGGTC 0.119 0.122 0.808 0.833 2.391 41 16 4677977017822052 7878 ATCACCCCCASTAGGGCATTC 0.000 0.000 0.683 0.759 1.068 41 1646793078 8050120 7879 GCCTGGGTTASAGGTGTCGTC 0.219 0.207 0.219 0.7901.371 41 16 46799880 17822471 7880 ACCACTCCCCRTGTTGCCGCA 0.214 0.2100.297 1.029 1.282 41 16 46806419 8047091 7881 TCTCCAATAGYTTCCTTTCCT0.849 0.788 0.474 0.491 1.273 41 16 46815699 17822931 7882CTTACTGGCCYGAGTACACTG 0.322 0.325 0.892 1.169 1.134 41 16 4682256111863414 7883 CGTGTGATTGYCAAGGTCCAA 0.887 0.888 0.834 1.079 1.338 41 1646829887 16946014 7884 AATGGACTTTYAACTCCTTCT 1.325 1.311 1.628 1.2431.011 41 16 46840944 17750580 7885 GTCTCACCAGSTACTCTGTTT 0.042 0.0431.488 1.202 1.061 41 16 46852072 16946039 7886 TGCTCCAGTARTGGTTGTTTA2.537 2.384 1.443 1.427 0.996 41 16 46865588 11076561 7887TCCTCCATTARTGTAAGTGCA 0.626 0.619 1.094 1.293 1.100 41 16 468671109931294 7888 TTGATTGTGGWTGAAGCAAGA 1.002 0.990 1.502 1.212 1.065 41 1646891036 — 7889 TATGGGAGCCRTGTAAATAAT 0.045 0.047 0.891 1.028 1.059 4116 46893998 9302757 7890 CACACATAGCRTCTTTTTCAC 2.050 1.950 0.942 1.1561.065 41 16 46909639 9933211 7891 TCAATTTAAARTGCATCAAAA 0.185 0.1750.769 0.815 0.999 41 16 46918683 — 7892 AGAGCTGGGAKATACCTCTGC 0.8640.832 0.995 0.807 0.907 41 16 46925991 13338978 7893ACATCATCTTSTTGCAGTTGT 0.389 0.382 0.566 0.766 0.944 41 16 469356158055028 7894 TGTACTTCATMATTTCTGAGG 1.282 1.241 0.603 0.773 0.689 41 1646981974 17825174 7895 CTCATTCATCRTGTCTTACAG 0.034 0.034 0.598 0.4350.687 41 16 46985312 — 7896 CTGGTCATCARAGCAGTTTAA 0.276 0.272 0.4790.436 0.874 42 16 73578487 9925422 7897 TGCCTGGACCRTTTCATGTTT 0.5570.559 0.496 0.801 1.481 42 16 73585421 9925758 7898TGGGCTGTTCYCTTTGCCTGG 0.706 0.706 0.214 0.559 1.511 42 16 7360368516952761 7899 GTTGTAGTGTRAAAGGATCAT 0.361 0.363 0.515 0.599 0.935 42 1673608361 9925180 7900 AATAAACACARTGAAGTACAG 0.019 0.020 0.584 0.3940.742 42 16 73626020 4888334 7901 TCCGTTCTTTYGATTTTAACT 0.880 0.8560.531 0.584 0.491 42 16 73638451 16953733 7902 ATAATCTGTCYCTTGCCATTA0.718 0.727 0.418 0.563 0.747 42 16 73644376 17684886 7903GCCACTGGGGWCATGATGTGA 0.622 0.578 0.740 0.417 1.272 42 16 736487727196872 7904 GAGTTTTTTTWAATATATTGA 0.106 0.105 0.569 0.433 1.357 42 1673665578 11149795 7905 CTGTCCGTGASCGTTGCTAGA 0.760 0.753 0.330 0.9731.492 42 16 73696428 2287989 7906 GGCAGAGGCCRTCAGACAGGT 0.000 0.0000.292 1.647 1.300 42 16 73701798 1043503 7907 GGCCTAGCACRCTTTGATGAG0.237 0.232 0.953 1.487 1.646 42 16 73722875 7206259 7908AGAAGCATCAYGTTGCTAACA 0.447 0.447 1.846 1.535 4.488 42 16 7376194412935567 7909 ATATATCCAGYTGTAAATAGC 1.466 1.440 1.769 1.777 4.488 42 1673762714 17673793 7910 CAGAGACTTTRGAGGAAAAAT 2.322 2.561 2.007 1.9203.604 42 16 73787731 1559362 7911 TGGTTTTGCCYGTGTGACCAA 0.345 0.3541.928 4.267 3.687 42 16 73789787 10514392 7912 TCTTGCGACTRCTCCAGACGT0.797 0.804 1.616 4.488 4.187 42 16 73812718 8051363 7913TTCTGCTTCCRATGTGCCATT 0.137 0.138 2.619 4.364 4.488 42 16 738232208062565 7914 TCTGAAAACAMGGCGATCCCA 0.865 0.862 3.503 4.187 4.488 42 1673837879 28439846 7915 GGTCAGAGACRGCCTCCCCAA 4.037 3.761 3.447 2.8324.364 42 16 73851712 4261573 7916 TTTGATTCGAYTGATGGCTAC 0.737 0.7353.623 3.275 3.886 42 16 73865731 4888370 7917 TGTGTGTTTCYCAGCCCCAGG0.687 0.706 3.852 3.091 2.855 42 16 73878532 4243112 7918CTGAGCAACCYTTCAAAGGTG 1.299 1.294 1.176 3.114 2.189 42 16 7388488317605723 7919 AACAAGCTGCRAAGGGGCCTG 0.047 0.047 0.994 2.639 1.941 42 1673890482 11149814 7920 CACCAAGAAAYCTGTGTAATG 0.934 0.924 0.830 1.2842.107 42 16 73897232 17674257 7921 GTGAGTTGGGYCCTGTTTTTT 0.530 0.5260.870 1.135 2.101 42 16 73903741 9923574 7922 ATTTTTAAACSTTTTGTGGCT0.374 0.382 1.099 0.865 2.082 42 16 73908476 2865532 7923ATCTTCAGCARAGTCCTAGAT 1.474 1.435 0.942 0.870 1.233 42 16 739175978046109 7924 TTGTGTGCCARATGAGTTTTT 0.708 0.724 0.699 0.849 1.166 42 1673927374 8052405 7925 GACTGACTTCMAAATGACAGA 0.508 0.495 1.018 0.9431.013 42 16 73935915 9972714 7926 TATTTTATAGYAGGCGTGTAT 0.010 0.0090.489 1.015 0.990 42 16 73945935 8050769 7927 CCTCATGATGYATAAAAACAT1.194 1.151 0.670 1.103 0.998 42 16 73955589 — 7928TCTGCCAGAARGAAAATAAGG 0.024 0.024 0.855 0.756 1.154 42 16 739624758057203 7929 CACATGTTCAYGTGGACTTCA 1.233 1.192 1.206 0.854 1.093 42 1673969226 8046184 7930 CCCAAACTGCYTTAGCAATAA 0.850 0.862 0.710 0.8021.146 42 16 73980404 10514396 7931 TTAAGTTATAYTGCTTTAGGT 0.820 0.8051.078 1.334 0.905 42 16 74008671 7188604 7932 TATATAATAGKTTATTCACTC0.074 0.075 0.724 1.069 0.944 42 16 74033952 4888426 7933ACAGTCTTGGRGTCTTCTGTC 1.005 0.982 1.013 1.208 0.989 42 16 7404063312928036 7934 AGGTATGATCRCTATTCTCAA 0.325 0.324 0.925 0.892 1.312 42 1674047910 247451 7935 AAAAAAGTCASCCTTAGTCAA 1.571 1.579 1.302 0.870 1.11742 16 74052777 11641430 7936 GATGTCTACCYAACCCTCCGT 0.491 0.488 0.7310.813 1.260 42 16 74063566 12051137 7937 CTAGGTGAAASTTTTTGAAAT 0.6030.570 0.954 1.253 1.024 42 16 74074404 — 7938 CCAATGGGGCRCCGAAAGAGA0.035 0.036 0.385 0.979 0.857 42 16 74088978 2550907 7939ATATGACTTAYCTATGGCTTC 0.640 0.635 0.634 1.054 0.973 43 16 764456597198177 7940 TTGAAATTCTYGGCACCTGTG 0.405 0.397 1.027 0.653 1.335 43 1676452281 12599959 7941 TAGGAAGTTTMAAGCCTGCTT 0.089 0.090 0.806 1.1151.641 43 16 76465091 1125690 7942 CATTAAAGCAYAAAGGAAACT 1.939 1.7980.634 1.536 2.082 43 16 76470467 3751767 7943 TTTCAAGATTYCTCCAGTTCC0.000 0.000 1.297 1.887 1.900 43 16 76476485 12924014 7944TCGGTGTGGTYACAAGAGCAT 0.327 0.344 2.016 2.058 2.032 43 16 76484142382587 7945 CCAGCACAAAYGGTTTCTAGT 1.834 1.960 1.535 2.308 1.872 43 1676491397 16946778 7946 AAGCTACACCRTATGCAAGGC 1.167 1.173 2.131 2.4601.683 43 16 76499090 17704468 7947 ATGTTCAGTTYTATATCCCCA 1.164 1.2482.441 1.834 1.838 43 16 76508672 1429066 7948 TGGAAGCAACRAAAAAGGAAG1.021 0.990 1.412 1.851 1.714 43 16 76517193 372627 7949GAGAAATCTAYCAAACAACAT 0.610 0.608 1.336 1.722 2.607 43 16 765234239937770 7950 CCTGAGCATTKAGTTCATGGG 0.367 0.373 0.738 1.306 1.976 43 1676529180 7195268 7951 AGGCGAGTTGRATCCCTTGAA 0.916 1.008 0.323 0.9522.484 43 16 76538629 10454707 7952 AAGACCATCTSCTTCTAGTGC 0.073 0.0730.568 1.480 2.635 43 16 76546435 — 7953 CATATTGTGTYCTGTTCATGA 0.0350.035 0.504 1.289 2.103 43 16 76555316 16946919 7954AAGACCTTCARAACAAAACCG 1.193 1.212 1.157 1.570 1.901 43 16 76566465373929 7955 TTTTAGGGTTRGCACCTGACA 0.223 0.226 1.448 1.646 2.012 43 1676574323 402904 7956 GCCATTTGGGRTATAGGAAAC 2.522 3.060 2.149 1.480 2.17043 16 76581622 — 7957 CAAAAAAATAYAGCCAACATA 0.591 0.568 2.057 1.5852.071 43 16 76587405 2252113 7958 CTGACTTGCCYGGACACGTCT 1.416 1.4782.450 2.219 2.038 43 16 76594303 8058458 7959 CAATCCTATCKGTGAATTTCT0.863 0.854 1.058 2.397 2.139 43 16 76601272 11863422 7960GCTCTGCGAGYGTTTCCTGTC 0.545 0.541 1.638 2.261 2.955 43 16 7660782212597064 7961 GTAAGCCCAARAAGCCCCATA 0.066 0.066 1.821 1.770 3.026 43 1676612446 11643023 7962 CCACTGCCCTYCATTTGCACC 1.697 1.622 1.542 2.0853.342 43 16 76634316 4888726 7963 ATTGGGGCCAYCCTGTGCATT 1.726 1.7841.530 2.924 3.503 43 16 76647688 8045975 7964 TCACTTGCCASCTTGGCCAGG0.932 0.921 2.098 2.779 3.225 43 16 76660674 6564507 7965CTTGTCATTCRATCTTGTCTA 1.063 1.076 3.221 3.210 3.233 43 16 7667317516947096 7966 TCAGGAACGAYGATTTATAAC 1.284 1.194 3.163 3.585 3.153 43 1676680185 — 7967 GGGCCTATGCRTAGAGTCAGT 3.976 4.568 4.665 3.988 3.386 4316 76685815 1073111 7968 CTCCTTGCTARTAATCAATGT 0.167 0.170 3.275 3.2453.082 43 16 76698607 8045088 7969 CCCTTCGAATRCCTCTTGTAG 2.072 2.0793.924 2.949 3.071 43 16 76710658 11645006 7970 CACGGTGAACRTTACATGCAA0.665 0.686 0.930 2.815 2.809 43 16 76718209 9319518 7971TTAAACTTGTSTGGCTTTGCA 0.275 0.289 0.943 2.189 2.232 43 16 7672582928607706 7972 TTGAGCCCCTYTTCTCTTAGT 0.284 0.287 0.432 0.597 1.841 43 1676738796 17650073 7973 GGAACTCTGGSCGTTGATGTA 0.175 0.178 0.260 0.6421.675 43 16 76746301 12716853 7974 ATGTTTCCTGRATAATGTGCA 0.879 0.9110.146 0.135 1.363 43 16 76762452 7206823 7975 CCTCATTGATWAGCATCGGAA0.121 0.126 0.155 0.054 0.360 43 16 76769919 7194833 7976GAATACATGTSAGTGCTAGAC 0.022 0.023 0.123 0.088 0.334 43 16 7677551717573298 7977 CCTTCCACCAYATCAGAAGGT 0.336 0.333 0.004 0.106 0.092 43 1676782865 6564516 7978 TATGCCGCCAMAATATATCAA 0.041 0.040 0.055 0.1420.067 43 16 76790899 7192037 7979 CTGCGGATCCRGCATCATGAC 0.020 0.0200.143 0.037 0.056 43 16 76797626 9938637 7980 AGTTATTGGTRGTGGAATATC0.684 0.704 0.154 0.047 0.082 43 16 76803679 2194292 7981AGGCTGATCARTCTAGGCTGG 0.429 0.429 0.171 0.082 0.111 43 16 768117512067714 7982 TTATTTTTCCRAGCAAGTTTT 0.370 0.374 0.231 0.062 0.041 43 1676822238 12931696 7983 CACACCAATGRTATATATTTT 0.032 0.032 0.124 0.1520.125 43 16 76827833 7204887 7984 TGACAGACACMTTAAGAGCTT 0.253 0.2600.081 0.258 0.304 43 16 76830780 11649150 7985 TCATTCATGCYGAGACCCTGA0.404 0.388 0.136 0.118 0.250 43 16 76868733 4380066 7986AAAATGATGTMAGGATCCTTT 0.241 0.242 0.291 0.262 0.387 43 16 768769194887950 7987 ATGAAGCCCCRTAGGATGGCT 0.621 0.625 0.236 0.518 0.496 43 1676894438 8051705 7988 ATAGATGCTCYTATTGGAACA 0.515 0.518 0.546 0.5200.403 43 16 76904717 4887952 7989 TAGAACGTCTRAACAGTGCTG 0.062 0.0641.024 0.687 0.590 43 16 76920881 8048594 7990 CTTGAGGCTTYGTGGCAAATG1.143 1.130 0.705 0.717 0.580 43 16 76933108 7498411 7991AGCATCGAGTRTGGAAACCTC 1.213 1.178 0.809 0.701 1.001 43 16 769459654587988 7992 ATATGCTTAGRAATATCCAGC 0.029 0.029 1.038 0.927 1.123 43 1676959950 4459554 7993 ATATCCTCAAMCCAAGGAGAA 0.772 0.763 0.591 0.8551.104 43 16 76971907 8058540 7994 CCATTCAGCCSTATCAGTGAA 0.467 0.4480.632 1.409 1.083 43 16 76984378 8058511 7995 CAGCGGTCGTYGGCCAGTGTG0.286 0.291 0.767 1.238 0.966 43 16 76997097 8051395 7996CAGGAAATATYCTCTTTTAGG 1.263 1.292 1.174 0.948 0.956 43 16 770113686564541 7997 ATGAGCATAARATCTAAATAG 0.000 0.000 1.303 0.996 0.987 43 1677024259 3764342 7998 TCTCAGTAATMACATTGTCCA 1.555 1.575 1.386 0.8450.766 43 16 77031763 4297688 7999 CTAGAATACTSCTCCTCCACT 0.974 0.9720.908 0.877 0.628 43 16 77050888 2738646 8000 CATGCGGTATSTGCCATTTTG0.240 0.236 0.807 0.823 0.634 43 16 77056971 2738664 8001TTTCATTAGTRACTCACATGG 0.380 0.383 0.419 0.524 0.831 43 16 7706385916947630 8002 TTCTCTTGCTKTTCTCTAAAG 0.184 0.176 0.115 0.592 0.791 43 1677070054 2667556 8003 CGTTGAGTTASTGGTTCACTG 0.000 0.000 0.109 0.2120.893 43 16 77076412 2738696 8004 TTATAGATGARGTTCCCTTAG 0.113 0.1150.151 0.413 0.665 43 16 77082641 12444091 8005 CCCTCTCAGTYAGCTGCTACA0.211 0.231 0.113 0.397 0.769 43 16 77088538 2667590 8006TTAGGTGTATSTCTGCTCATG 0.561 0.569 0.596 0.510 0.599 43 16 7709556713331582 8007 TAATAAATAASTGAAATACCA 0.106 0.104 0.652 0.492 0.870 43 1677102849 1465099 8008 CGTTGCAGGCRCGTTGAATGT 1.715 1.653 0.926 0.5931.110 43 16 77109437 11641340 8009 TATGTCTTTGRGCTTGGTAAT 0.266 0.2600.725 0.856 1.201 43 16 77115457 16947928 8010 GGTGATTCCARTGTGAGTGGT0.811 0.780 1.108 1.703 1.520 43 16 77121333 11645676 8011TGTTGGTTTCYGTGTTGATGA 0.177 0.169 0.577 1.893 1.742 43 16 7712774516948025 8012 AAGGGTGTGAYCAGTTCCATG 0.779 0.718 1.591 2.106 2.399 43 1677135652 16948047 8013 GTAAAACAGTRGATGTCTTTC 0.751 0.736 1.613 1.8042.454 43 16 77145092 2667505 8014 TGATAGAGAASCTTTCCCTAG 1.949 1.9791.867 2.366 2.073 43 16 77154108 2859627 8015 ACAATCTGATYGCTCCCCTGA1.011 0.979 2.064 2.628 2.599 43 16 77156356 9928690 8016TTTTAACAAGRCAAGTAGAAA 0.731 0.730 2.423 2.560 2.912 43 16 7717110516948109 8017 AGAACTATGTKTGTTTTCACT 1.115 1.104 1.902 2.016 2.977 43 1677180388 11862167 8018 TTGCTGGGAAYGTGGCCATCA 1.201 1.210 1.587 2.1892.720 43 16 77188320 17640520 8019 ACTCTCTTGAYGCTATCCTCA 1.391 1.3841.157 1.968 2.653 43 16 77206499 12599563 8020 AAATTCTATGKGTAAAATAAT0.071 0.071 1.117 1.805 2.184 43 16 77213355 2738566 8021TTGAAATTGTMCTGGTTTTTC 0.160 0.158 1.448 1.775 2.108 43 16 772191652550608 8022 GAAAAGTTGASTAGACAAAAG 1.018 0.984 0.882 1.479 1.667 43 1677230099 2550620 8023 TCAGATTCCAMTCGAATACAT 1.920 1.920 1.254 1.0011.628 43 16 77238229 2548841 8024 TTCCTTTTATYTCCCATCAGC 0.274 0.2601.394 0.712 1.444 43 16 77245970 — 8025 GATTCTGATTKCTCCGTGATA 0.4780.485 0.866 1.063 1.192 43 16 77256370 17795127 8026AATGAACTTGKGAAGGTTTGC 0.541 0.536 0.278 1.279 0.846 43 16 7726290217722185 8027 CCAGTATAGAYAACTTGTGAT 0.017 0.017 0.486 0.909 0.534 43 1677268743 6564575 8028 ATGTTGAGATYACATACATCC 0.656 0.654 0.509 0.3170.527 43 16 77276578 2738630 8029 AGGACTGGGASAGATCTGTGA 0.769 0.7850.393 0.283 0.598 43 16 77284472 7195153 8030 CTTGCCAGCARCCTTTTAGAT0.564 0.527 0.502 0.240 0.763 43 16 77289752 — 8031TTTTTGGGGTWTCTTCCAGCC 0.253 0.266 0.278 0.137 0.476 43 16 773107662042433 8032 TAAATCTTCTRTCAGGTGATG 0.281 0.282 0.146 0.218 0.566 43 1677322980 8052934 8033 TTTATTTGGGYTTTTTCGGTT 0.112 0.114 0.045 0.4850.499 43 16 77330775 16948419 8034 CAACAATTTTKGAGGCACCCA 0.291 0.2970.062 0.501 0.424 43 16 77337515 — 8035 GTGATTTGTCSAAGGACATCG 0.0460.044 0.499 0.529 0.460 43 16 77344287 12599398 8036TCCTGGTCCCRTCTTGGCTGG 0.396 0.412 0.860 0.515 0.334 43 16 7735338610514443 8037 GTGCAGTGAGRATGAATCAAC 1.645 1.755 1.113 0.482 0.270 43 1677360990 7201295 8038 TTTTTACCATRTCTGTTACTC 0.819 0.829 1.210 0.5070.261 43 16 77368476 4887985 8039 GAGGAAAATTRGGAATGAAGT 0.621 0.6310.994 0.429 0.351 43 16 77375785 7191931 8040 AATCAGAAATYTGTGACTCCA0.228 0.231 0.329 0.577 0.406 43 16 77382825 2550595 8041CTCCFGAAACRTAATTTGGAG 0.175 0.177 0.091 0.615 0.407 43 16 774017691124597 8042 ATGTGGACACRTGCAGCCACA 0.189 0.191 0.032 0.309 0.597 43 1677439717 7501409 8043 TTCAAGTCATRTTTGCCCCCA 0.039 0.040 0.077 0.2330.826 43 16 77453496 8063104 8044 GGGTGTGTCTRTATCTATACA 0.000 0.0000.249 0.118 0.833 43 16 77461185 16948659 8045 CTTAGCAGAGYCAAAAGCCAG0.518 0.486 0.414 0.282 0.476 43 16 77470888 2656620 8046CCCTGGTCTTMAAGTCCACTC 0.732 0.745 0.448 0.489 0.366 43 16 774784024887990 8047 TTGAGTTTTCRTATTGTCAGT 0.534 0.559 0.669 0.595 0.293 43 1677484117 11647676 8048 ATTGTGATACWATAGGAGCCG 0.087 0.091 0.810 0.7410.267 43 16 77491900 8064053 8049 TCAAGAAACCSACAGAGGTCC 0.969 0.9600.678 0.691 0.254 43 16 77497905 16948804 8050 CACTTGTGCTSTGCAAAATCA0.799 0.818 0.632 0.615 0.236 43 16 77503965 8060900 8051AACCAGCAGGRTTATATCCCC 0.494 0.482 0.768 0.409 0.234 43 16 775113587192071 8052 TACCAAACACRTAAGCTTATT 0.461 0.456 0.443 0.278 0.288 43 1677528453 16948943 8053 ATTAAGTGGARATATAGGTTT 0.358 0.355 0.184 0.2600.487 43 16 77535072 13331318 8054 AGTGTAGGACRCTGGAAAAGT 0.287 0.2770.093 0.096 0.370 43 16 77541656 1995548 8055 GCTCAAATGCRTGACATGATG0.153 0.152 0.028 0.086 0.442 43 16 77549079 9319530 8056AAGGGATTTTSAAGCAGTTGT 0.112 0.106 0.008 0.270 0.432 43 16 775570508052893 8057 TAGCATCTCTRGCATAAACTA 0.004 0.004 0.050 0.212 0.814 43 1677563991 4888896 8058 GTTCCATTTAMATTGAATGGC 0.089 0.086 0.443 0.3311.032 43 16 77570962 8063569 8059 GCTCCTGTGASGGTTTCTACA 0.712 0.7340.482 0.270 0.897 43 16 77576754 16949152 8060 GTTGTCTCCARTGTGCCTTGT1.472 1.505 0.909 1.028 0.849 43 16 77583096 10492907 8061CTTTCCTATAYAACGCAAGCC 0.206 0.201 0.908 1.856 0.861 43 16 7759324510492905 8062 CTGCAGTGGTRTCGCTTTAGA 0.850 0.835 1.943 1.790 0.967 43 1677601455 1469135 8063 AAGCTGCTATSCTTGCAAAGG 0.019 0.020 1.800 1.8881.053 43 16 77609347 12932339 8064 AAAAGCAGGCRAAATAGAATA 2.388 2.4541.643 1.743 1.421 43 16 77614961 16949262 8065 ACCATCCACCRCAGGCAAACA1.561 1.536 1.436 1.248 1.501 43 16 77621304 2656645 8066CATAAACGCAYATGGTCACTT 0.160 0.156 1.623 1.336 1.433 43 16 7762795416949326 8067 CCTGAATTATYGGAGCTCTTG 0.234 0.230 0.713 1.460 1.203 43 1677634311 2194344 8068 TAAGTGATGCRTCAGAATTTC 0.371 0.391 0.213 1.5120.967 43 16 77641440 16949415 8069 GGAATGGGAGSAGTTTAAACA 0.605 0.6040.508 0.509 1.009 43 16 77648924 9928080 8070 GAGCTGAGCCRCACTTGGAGA0.406 0.418 0.477 0.115 0.942 43 16 77661543 17726900 8071AGCTATTTCCSTAATACCAAG 0.883 0.847 0.306 0.298 1.112 43 16 7766734311150133 8072 TCTGCTCTAAYGTTGGATCAT 0.170 0.168 0.141 0.349 0.472 43 1677674005 6564644 8073 CCAGAAAGTCSCGAGTGTTTT 0.061 0.041 0.326 0.3660.336 43 16 77681077 17727687 8074 CTTCGAATTGKTGCACTAGAG 0.112 0.1080.175 0.331 0.918 43 16 77687044 8052725 8075 AGTAGGCTCTMACCTGGCAGG0.920 0.915 0.256 0.293 0.977 43 16 77690771 17727813 8076GTAAGGTCCCRTGACAATTAG 0.415 0.408 0.447 0.325 0.873 43 16 777063667185485 8077 AGTTAGACAAYTAGCTCGCCC 0.475 0.467 0.504 1.078 0.690 43 1677713723 2005036 8078 GTTCAGGCGGYATTAACCCCC 0.510 0.486 0.548 1.2600.770 43 16 77720121 12923469 8079 ATTTGCCAAGKTTTGAAAAGT 0.243 0.2511.521 1.252 0.644 43 16 77726730 4611476 8080 TCCTTTTCCCRTAATTTTATG0.985 1.004 1.511 0.853 0.703 43 16 77729566 1424113 8081GTCCTTGATCRTTTTTCTTGG 2.263 2.155 1.238 0.965 0.798 43 16 777428947201082 8082 GTAACCAATAYCGCTCCTTCT 0.391 0.396 1.038 0.692 0.793 43 1677751639 6564651 8083 TTTTGATGTAYAGACATCCCT 0.108 0.107 0.926 0.8420.914 43 16 77757915 11150140 8084 CATACGGGCARTAGTCCGCAT 0.084 0.0850.311 0.872 1.064 43 16 77764739 6564653 8085 TGTATGTTTGSGGTACAACTC0.811 0.795 0.284 0.628 0.929 43 16 77770740 12716871 8086GAGAGGTACTSTTAGCAAATC 0.374 0.387 0.317 0.396 1.054 43 16 7777716117730134 8087 AGAAATGAGARTGAGGATAAA 0.336 0.343 0.332 0.575 0.986 43 1677783493 386497 8088 CATAGGGTATYTCCAGATAAT 0.321 0.313 0.527 0.565 0.84643 16 77789947 383673 8089 GGAGCAACCTMACTAGAGACA 0.163 0.166 0.837 0.8350.411 43 16 77797274 449842 8090 CGCACCTACAYGCGTGGGAAC 1.332 1.396 0.7100.619 0.596 43 16 77804563 395050 8091 GCAAGACGGARAAAATGCTTC 1.114 1.1530.990 0.644 0.599 43 16 77810494 270425 8092 TGCCCTGCTGMATCTTAGGCG 0.0550.057 0.992 0.576 1.027 43 16 77829050 9924583 8093TTCTTAAACTMTGATCTATCC 1.096 1.105 0.606 0.897 0.952 43 16 7784183716950049 8094 TTTTTGGCACKTGGCTCTTTA 0.001 0.001 0.173 0.917 1.094 43 1677849307 437620 8095 ATTTTGCCCARATGTTATCAG 0.470 0.483 0.745 1.195 1.16243 16 77856543 270433 8096 TGCATAAATGRAAGTCATTAT 0.056 0.054 0.291 0.9031.285 43 16 77863078 17796919 8097 TCCATCTCTAYTGCTAAGGAA 1.399 1.3931.302 1.262 1.563 43 16 77869153 4888944 8098 AATTTGAGACMACTTTTCTGT0.102 0.096 1.230 1.066 1.195 43 16 77875406 — 8099TAGTAGTGATKGATGGCAGAA 1.910 1.878 1.862 1.400 1.094 43 16 7788172817724291 8100 TTTCCCATAARTCATACGGGC 0.411 0.401 1.278 1.488 1.226 43 1677895943 9924169 8101 AGAACTAGCCYTTTTGATTCC 0.694 0.856 1.571 1.4800.981 43 16 77902014 12600246 8102 CCGCCTCCGCSACATTTTGCA 0.628 0.6380.704 1.178 1.189 43 16 77908301 12596374 8103 TGGACACCAAMAAAGTAGGAG0.617 0.543 0.642 1.308 1.016 43 16 77914236 16944185 8104CCTGCCAAAGRGTTCTAGTTT 0.482 0.484 0.633 0.665 1.033 43 16 779213157191820 8105 CATACAGCATRAAGAACAGAC 0.336 0.308 0.497 0.740 0.725 43 1677927430 6564662 8106 CACAGGGCTGSGCTCAGGGCA 0.799 0.808 0.477 0.4710.750 43 16 77933390 16950304 8107 TATGAACAACRACAAAATTTA 0.321 0.3410.531 0.321 0.689 43 16 77939410 7198888 8108 GTCCTTTCAAKGATGTCTCTA0.511 0.492 0.422 0.293 0.687 43 16 77946548 9936888 8109CTATCTTGCASGATCCTAGTG 0.633 0.629 0.183 0.231 0.493 43 16 779600294243168 8110 ACCAGTAGGASCCCTGGTGCT 0.033 0.034 0.206 0.601 0.493 43 1677970762 12325433 8111 TGACAGTCTASTTTGCAGTTG 0.110 0.111 0.118 0.4740.381 43 16 77999427 4530155 8112 AGAGGCGGGCSCCATAAGAGA 0.412 0.4290.475 0.388 0.423 43 16 78006279 16950383 8113 TTCATGTGTTKTCTTAGCATT0.175 0.182 0.636 0.432 0.550 43 16 78012972 8051128 8114CGGAAGGGAARATGATAGACA 1.680 1.645 0.620 0.277 0.403 43 16 780230634888977 8115 GAATGCTAACYTGTGTTTTCC 0.395 0.392 0.733 0.469 0.336 43 1678030355 7190849 8116 GAGACATCCTMAGATTAACAA 0.098 0.096 0.668 0.7100.317 43 16 78037195 11150162 8117 TTTATTATTAYAATTGCTCCT 0.617 0.6340.247 0.630 0.219 43 16 78049764 1862720 8118 CTATTACCAAMAGAAGACTAC0.113 0.109 0.420 0.575 0.214 44 18 22503963 17607947 8119GGATGAATTCRGTTCTCTGTG 0.195 0.193 0.223 0.085 0.019 44 18 2251785317608457 8120 TATCTGATGCKAATGGTATAT 0.886 0.862 0.201 0.042 0.021 44 1822524486 — 8121 GATATGTCACWGAGTAGGATA 0.561 0.579 0.256 0.030 0.037 4418 22530423 16942566 8122 GCAGAGTTATRGGAGACTTCT 0.097 0.091 0.198 0.0280.025 44 18 22535819 16960655 8123 ATATTTTTTAYGGGAAAAAGC 0.117 0.1190.028 0.051 0.016 44 18 22543855 470306 8124 CCTTCTCCAAYGGCTTAATAA 0.0180.018 0.001 0.091 0.011 44 18 22549985 470677 8125 CACTTCTCTCKTGTTGGAGCT0.067 0.067 0.005 0.031 0.015 44 18 22555942 470455 8126TGTTTATGCTRATGAAGTGAC 0.094 0.091 0.035 0.010 0.018 44 18 225636067234618 8127 TGGGTAGAATYGAGAGAAAGA 0.160 0.312 0.076 0.009 0.020 44 1822569929 4800265 8128 AGGTTTTCACRTGATGCTGTG 0.479 0.474 0.111 0.0130.091 44 18 22579380 — 8129 ACAAGGAAGCRGGCACTGGGC 0.314 0.310 0.1010.013 0.071 44 18 22595324 — 8130 TGTGAGCAGCSCTCAGTAAAG 0.195 0.2100.089 0.021 0.095 44 18 22608012 1562701 8131 CTCCCTTGCTRTAATAAGTGC0.070 0.070 0.026 0.313 0.185 44 18 22613927 573998 8132CCTAACACGTMATTTGGTGTT 0.281 0.249 0.014 0.333 0.516 44 18 22621971487455 8133 TCTTGAAAAGYGGTGAAAATG 0.040 0.039 0.477 0.303 0.596 44 1822626379 621765 8134 AGCCCCAAACRTTAACTTCTT 0.173 0.171 0.620 0.483 0.86544 18 22640533 16942713 8135 GCCTGAAGAAKCCTGAGAATG 2.015 2.137 0.6691.042 0.766 44 18 22647211 16942751 8136 GCTGCCTAACMAACATTCCCT 0.3910.383 1.170 1.180 1.204 44 18 22654052 17690930 8137TTATTTTACCRTCCTGAAAGT 0.343 0.342 2.014 1.487 1.131 44 18 2266046811659932 8138 TGAGAGTGGGYGGACATGTAG 0.924 0.948 1.160 1.490 1.155 44 1822667416 16942801 8139 GTGCAGACCGWTAAAGACACA 1.717 1.688 1.571 2.3671.487 44 18 22673906 16942835 8140 TTTGGGGAGAWCACATTCCAG 0.383 0.3891.377 1.399 1.479 44 18 22684849 12455617 8141 CTTAATGTTGMCTTAAACCCA0.937 0.921 1.872 1.379 1.708 44 18 22692538 11661256 8142TGAGCCTTTAWCTTCTCTATG 0.091 0.094 0.836 1.619 1.836 44 18 2270301811662318 8143 CTGTGCCCCAYGCCCCACAGA 1.734 1.632 0.781 1.290 2.118 44 1822710424 589598 8144 TATGTTGACAYCATACTAGGA 0.105 0.108 0.844 0.898 2.09044 18 22715430 3974646 8145 TGATGAGGAAKGACACTGTTT 0.255 0.262 0.8701.011 1.894 44 18 22733560 630285 8146 CCAGGTTTGCRTGCCTACCTA 1.106 1.0750.518 1.584 1.681 44 18 22743410 162632 8147 TTAATTCTGAYACAGGTCGTC 0.1740.165 0.770 1.587 1.370 44 18 22749587 1143734 8148AAGAAGGTTGSTTTTGTTGCT 0.885 0.851 1.752 1.077 1.455 44 18 2275649116942955 8149 AAAGACATGTRACTACAGATT 0.797 0.749 1.414 1.125 1.388 44 1822763991 163221 8150 CTGGATTAAGYATCTACTGGT 2.722 2.809 1.313 1.324 1.35044 18 22770692 11663286 8151 GCAGAGAAGGWTATGATTTGA 0.221 0.218 1.1721.224 0.945 44 18 22777715 163048 8152 AAACAGTTCTRTAGAAGGACA 0.130 0.1271.213 1.361 1.048 44 18 22784157 16943030 8153 AACTGCTGTGYCCTGGGCCTA0.393 0.399 0.448 1.217 1.009 44 18 22790787 444673 8154ATGTTTGAAAWCTCTGGTTGG 1.104 1.042 0.576 0.960 0.807 44 18 227983001154215 8155 ATTCTCAACCKCCCACCTGTT 0.563 0.579 0.687 0.324 0.834 44 1822803905 1657316 8156 GTTATCATTAYATTTCTTGGT 0.524 0.513 0.496 0.3080.729 44 18 22804050 4093026 8157 GGTTGGGTGCYTAAAATTGCC 0.337 0.3240.270 0.314 0.591 44 18 22811160 1785548 8158 TAATTTGTACYGATGCTTAAA0.005 0.006 0.131 0.300 0.193 44 18 22826887 16943138 8159GTATTCATTTRCAGGCTCTTT 0.535 0.538 0.072 0.137 0.225 44 18 228338541154208 8160 GGTACTTAGAYGTTAAGTGGG 0.108 0.096 0.082 0.073 1.465 44 1822841488 16960687 8161 AGAGCAGATAYAATCACTGTA 0.255 0.239 0.154 0.0831.311 44 18 22851934 1436906 8162 GTTTCCTTAGMTTAAGCCTCA 0.359 0.3600.073 0.099 1.042 44 18 22867639 6508464 8163 CTTTTCTGGGYAATCTCATTC0.345 0.322 0.186 1.844 0.917 44 18 22874298 12604227 8164ATCACGTCTARGAAAATTTAA 0.173 0.169 0.252 1.449 1.277 44 18 228816747505854 8165 TATCACAGGARTAGTGGGTTG 0.588 0.588 2.626 1.426 1.364 44 1822892113 1485807 8166 AAATAGGAATRCTAGTCTTAA 0.453 0.440 1.938 1.4471.353 44 18 22898135 6508467 8167 AAACCCTGGGMAAGTGATAAT 4.907 4.4221.977 1.870 1.211 44 18 22905657 9957079 8168 CATAAATACAKTGTTGAGAAA0.098 0.095 2.126 1.886 1.292 44 18 22921346 12954928 8169ACTAGCAAAAYCACCTTTCTA 0.062 0.064 2.779 1.776 1.518 44 18 229280098088410 8170 TATCTGCAAARGGGTTATTTT 0.099 0.099 0.520 1.937 1.463 44 1822943704 919140 8171 GGGGCTCCTGSTGAATTATAA 1.810 1.771 0.523 1.938 1.51544 18 22952382 — 8172 TTACTTCTCCYTACATTATGT 0.000 0.000 0.494 0.4551.874 44 18 22959715 12969203 8173 CCTGAGAAGAYAGCCGTCAAT 0.065 0.0660.694 0.454 2.057 44 18 22966551 9964571 8174 GAACAAGGCARTACCTTGGGG0.091 0.112 0.364 0.670 2.011 44 18 22972845 4800790 8175GTAACAACTAYGAAAAAAAAA 0.492 0.484 0.230 1.285 0.533 44 18 229818004800792 8176 ATTCTGTTCTRTTGATTCAAT 1.154 1.118 0.465 0.725 0.775 44 1822991320 8096346 8177 TGAAATTAATRTGATTTGTTT 0.061 0.095 1.286 0.5951.009 44 18 23005211 9304504 8178 CAATCAAAAARTGGCTTATGA 0.708 0.7021.266 0.633 1.008 44 18 23011541 2195032 8179 CTTATTTGGTKTTCCCATTTT1.599 1.521 0.678 0.941 0.712 44 18 23016400 8088724 8180TTCAAGTACAYATCAATTATT 0.434 0.402 0.729 1.073 0.587 44 18 230271394438389 8181 ATTAAATACARCAAAATGGCA 0.187 0.184 0.889 0.685 0.703 44 1823036910 — 8182 TCACTGGTAARCCATGGCTGC 0.152 0.146 0.545 0.916 0.888 4418 23039432 — 8183 ACAGAGACTGRTAAGTATTTC 0.989 0.974 0.396 0.707 0.84744 18 23060734 17712675 8184 AAAGATCTTASGCAAACAGGA 0.832 0.866 0.6150.338 0.766 44 18 23070175 16943382 8185 GCAGAAAAGASACTGTCTAGG 0.0860.083 0.574 0.485 0.790 44 18 23075655 11664424 8186ACGTACTTTGRTTGTTTAAAA 0.757 0.714 0.362 0.546 0.886 44 18 2308171116943386 8187 CAAGTGTTATYTCCCAGGGGT 0.046 0.047 0.375 0.765 0.509 44 1823087741 16943406 8188 AGCTCTTACARGAATTGAGAA 0.000 0.000 0.516 0.5180.698 44 18 23102007 1426868 8189 AGAAATTATCRAAGTTCAAGT 0.883 0.8810.588 0.563 0.816 44 18 23112652 9954770 8190 AACATTAGCAYATTTTTAACA0.412 0.405 0.628 0.579 0.878 44 18 23126621 11661123 8191GTTTCCACTGYGTTGTTTTAT 0.857 0.870 0.865 0.745 0.694 44 18 231339061714636 8192 AATCTTCCAAMTCTTACAGTA 0.122 0.124 0.502 0.998 0.533 44 1823154769 17714238 8193 ATCCTTTGATMCTGATGCTAG 1.009 1.024 0.915 0.9240.607 44 18 23166163 7236330 8194 ACTACAATTTWCTTGGATTAT 0.112 0.1090.754 0.684 0.461 44 18 23172924 1474135 8195 ATAAAGAAATRAAACTGGACA1.171 1.180 0.942 0.641 0.484 44 18 23179814 4800802 8196AAAGAGGGTCRATGGCTTTTA 0.602 0.596 0.500 0.451 0.454 44 18 2318966210502490 8197 GACAATCCTGWTGGTGCCACT 0.449 0.417 0.561 0.462 0.471 44 1823202749 10502491 8198 CATGAAAAAGWGCAAAAAATT 0.232 0.231 0.243 0.2070.508 44 18 23210271 1519143 8199 CCTTACCTGARAGCCTGAATG 0.256 0.2560.113 0.269 0.366 44 18 23215939 10502492 8200 TTAGAGAAAASTTAAGATGAG0.343 0.326 0.059 0.225 0.363 44 18 23231527 1401466 8201TTATTCCTGCKGCCAGAGCTG 0.136 0.142 0.069 0.213 0.356 44 18 232452161401462 8202 TAATTCCAGGYGGCATACTAA 0.146 0.140 0.276 0.186 0.434 44 1823253013 6508486 8203 CTGGAGAGCCRCTTGAGAGTA 0.311 0.315 0.344 0.1510.212 44 18 23258373 9956638 8204 CATTCTAGCCYACCCCATGAA 0.989 0.9900.373 0.315 0.167 45 18 61324317 17777796 8205 TCTTTTAGATKTGTGGGTATA0.424 0.397 1.755 1.246 0.910 45 18 61336000 7233342 8206ATTTAATATGRCTTTTTTGCT 0.042 0.039 2.018 1.331 1.239 45 18 6134890617700174 8207 GGTGAAAATAYGCAACATTCT 4.001 3.392 2.394 1.260 1.374 45 1861359787 17074963 8208 ACTAACATTAYTGCTACTTAA 0.000 0.000 2.434 1.6081.289 45 18 61364918 9636017 8209 CTTCTTTTCAYCTTACTTACT 0.482 0.5042.536 2.156 1.442 45 18 61385547 10503100 8210 CATGAATTGAYACACATATTT0.075 0.079 0.432 1.951 1.562 45 18 61394512 17778833 8211CTAAAAACTGYAACTCAAGTT 0.147 0.152 0.813 1.853 1.649 45 18 6141202017779022 8212 CTAAATTGCTRTCTCAACTTG 1.084 1.076 0.811 0.767 1.476 45 1861422482 13380899 8213 AGTGACTTGCYAACTTTATTG 1.336 1.378 0.938 0.7901.361 45 18 61430893 17701273 8214 GTTTGTATTTYGATTGTGGAT 0.576 0.5951.101 0.834 1.360 45 18 61441000 — 8215 TGAAATAAATSTTGCCACGTT 0.5020.503 0.963 0.806 0.501 45 18 61451163 17075033 8216CTTCTCCACCYTATCATTTTT 0.000 0.000 0.654 0.793 0.626 45 18 6146035712604790 8217 AATTTTGTCTSTTCAACCTTA 0.559 0.555 0.395 0.552 0.559 45 1861469322 10871590 8218 TCACATGGTARATTTCCGTTA 0.700 0.743 0.249 0.2780.556 45 18 61475252 1942822 8219 TAGATGCAAAWAGTTCATTAG 0.004 0.0040.200 0.381 0.540 45 18 61479028 1942273 8220 TAAAGGACATRTATGGATTGC0.162 0.162 0.127 0.304 0.423 45 18 61496107 — 8221TTCTCAGAAAYAGTATCTTTT 0.213 0.217 0.210 0.246 0.221 45 18 6150497117708381 8222 CATGATTATCMATTTACTAAC 0.244 0.259 0.289 0.160 0.152 45 1861515106 17075147 8223 TCATGTTTGAWTGCAGAGAAA 1.082 1.059 0.296 0.0960.195 45 18 61522755 10515997 8224 TATTCATGTCKATTAAGGAGT 0.189 0.1980.254 0.114 0.226 45 18 61543876 17781554 8225 TTGTAAGAGGMCACTGACCTA0.103 0.104 0.288 0.101 0.323 45 18 61549981 9962601 8226GGAGTGTCTGSTTGCCTCTCC 0.054 0.055 0.014 0.237 0.451 45 18 615562887226378 8227 AAGCCCTTAARGAAATATAGA 0.000 0.000 0.007 0.296 0.480 45 1861565394 10084067 8228 CTTTGTTGTAYTCCCACATGT 0.017 0.017 0.170 0.3440.668 45 18 61582244 12961301 8229 GTATTTTTACRCTTCACTCTG 0.151 0.1530.355 0.634 0.876 45 18 61590121 8092259 8230 TTTGTCTTTTKTAAGATAAAT0.836 0.845 0.603 0.683 1.381 45 18 61595696 1484725 8231CTCCAAACGCRAAGCAAATTT 0.559 0.557 0.987 0.973 1.576 45 18 61601643990388 8232 ATGCACATATRCAACATAGGA 1.242 1.200 1.089 1.167 2.017 45 1861607583 17783081 8233 CTGTTTAGGCRTCTGTAATTG 1.621 1.613 1.363 1.6832.112 45 18 61613910 8097065 8234 ATAAATGCACYTGTCCTGAAG 0.195 0.1981.782 2.159 2.335 45 18 61621115 4334398 8235 TCGGTGAACAYGAAGCTCTAA1.218 1.162 2.446 2.730 2.366 45 18 61627292 11659738 8236TTGAAGTCCCRTGCATGTATT 1.382 1.349 2.981 2.988 2.505 45 18 616342774144764 8237 CCTATGGAGAYAAAGCCATAC 2.719 2.775 3.835 3.149 2.740 45 1861635487 6566179 8238 TAAGAGGTATYGTCATAACAA 2.465 2.467 3.723 3.1112.819 45 18 61672858 1484711 8239 AGTTCAAGGCYTTTTAGCTTT 2.709 2.6403.723 3.124 2.905 45 18 61678763 12967018 8240 ATTTTGAATCRTATGTTCGAA0.775 0.770 2.852 3.254 2.942 45 18 61686997 2587410 8241GGAAACTTTTYTTCTATATTA 1.996 1.843 2.131 3.173 2.840 45 18 616979732628210 8242 TTATCTTTGASAGTTGCTGAA 0.706 0.685 1.762 2.516 2.957 45 1861706203 7229176 8243 AGTAAAAGAGSCTTAAAAATG 1.541 1.516 1.515 1.7382.934 45 18 61717877 — 8244 TACTTCCCATYACTACATCTC 1.234 1.224 0.9621.245 2.911 45 18 61725718 17075520 8245 TAATGGATTGYAAATGGAAAG 0.0720.072 0.789 1.472 2.206 45 18 61738544 183260 8246 AAGAGCTACAWATTAATCACA0.174 0.214 0.410 1.243 1.677 45 18 61742429 2628245 8247CTATAAATAASCATGCCCTAA 0.262 0.262 0.684 1.301 1.134 45 18 617589019949357 8248 TTTCTAAAACRTATTTGAGCT 0.525 0.495 1.098 0.880 0.985 45 1861764669 6566183 8249 ATAAAGTCCAYGGTGGATTCT 1.949 1.818 1.225 0.4800.739 45 18 61771354 17075606 8250 TGGCCCCTTTYGCCAACGCCA 0.807 0.7921.158 0.466 0.660 45 18 61776985 6566186 8251 ATAAAATCGTRGGATTAGAGA0.463 0.455 0.879 0.422 0.403 45 18 61783512 2541870 8252ATGTGCCAGTWTGCTTTTATT 0.142 0.143 0.117 0.510 0.431 45 18 617909579945649 8253 TCCTGACTACSAGGGCAAGGA 0.009 0.009 0.010 0.439 0.473 45 1861792527 2541874 8254 AAAGGTCACCRTCATCTAGCA 0.041 0.040 0.028 0.0900.528 45 18 61804445 9962706 8255 AACTATGCTAYGACACCATTA 0.034 0.0330.044 0.187 0.498 45 18 61816573 9949391 8256 ATAATTGTTAYGCATTTTTGA0.631 0.613 0.083 0.162 0.626 45 18 61829609 2541743 8257TATATAGGTCRATGAAACAGA 0.250 0.250 0.504 0.219 0.301 45 18 618363602715320 8258 GCAGCAAAATRCCTTGGTCAC 0.259 0.247 0.628 0.237 0.186 45 1861842726 2706582 8259 ACTGCAAACCYCAATCTTATG 1.417 1.390 0.580 0.5240.258 45 18 61852405 10513920 8260 TAGGCGAGTCRAGATAGAAAA 0.271 0.2730.511 0.610 0.253 45 18 61859712 2541778 8261 AAAAACATTCWTCAAATTAAT0.000 0.000 0.897 0.459 0.280 45 18 61867908 4891639 8262AATGAGATTCYTTGGAAGATG 0.123 0.119 0.404 0.629 0.285 45 18 618745302715297 8263 AAACATAATAMAATCATTTTC 1.134 1.128 0.294 0.554 0.528 45 1861881775 995160 8264 TACTGAAATTYTAAAGTAGAA 0.294 0.286 0.496 0.246 0.49245 18 61888583 2706614 8265 AGTACCTATTRAGATGATGAC 0.017 0.017 0.4560.193 0.628 45 18 61897220 2541809 8266 CCTTCTTTCAYAGGCACAGAG 0.8820.889 0.140 0.431 0.609 45 18 61905727 12966332 8267GAATGGACTCYGCATTAACCC 0.002 0.001 0.075 0.506 0.434 45 18 6191644517279167 8268 GGTTGCTTTCYTTACTTTAAT 0.205 0.196 0.519 0.516 0.392 45 1861924877 2058842 8269 ATTGAAATACRAAAGAATACT 0.008 0.008 0.376 0.4760.429 45 18 61932124 12607652 8270 GTAAAGCAAASGACTATAGTA 1.520 1.4550.766 0.513 0.559 45 18 61945465 4439860 8271 GTGGTAGCCAKAATAATGGTC0.422 0.393 0.727 0.361 0.510 46 22 16451100 1296819 8272GCAATAGACTMCCACTAGAAG 0.035 0.036 0.014 0.000 0.006 46 22 164578145747252 8273 TCTACAGTTCYTTCATTTAGG 0.215 0.218 0.008 0.053 0.045 46 2216469866 5747268 8274 TCTGGTCTCAYTGGTCCCTGT 0.301 0.317 0.007 0.0770.061 46 22 16482520 — 8275 ATACCCACTCRTCTTAGTGCA 0.036 0.036 0.3320.114 0.064 46 22 16492758 5747302 8276 AAAGTCAAATRATTTCTATAA 0.0390.039 0.393 0.317 0.092 46 22 16506100 713701 8277 ATCAGGTTTTRTAAGAAAATT1.523 1.474 0.394 0.380 0.129 46 22 16518718 8140916 8278GCCAACATTTYATTTTTTAAG 0.381 0.386 0.824 0.339 0.327 46 22 165342585992786 8279 TAACATTTTCRAGTTTTTATG 0.287 0.287 0.919 0.367 0.521 46 2216546790 2587076 8280 TTCCTCTAGTSGTGACATGAA 1.042 1.007 0.283 0.4720.845 46 22 16558565 2587082 8281 ATAGCTCTTARTTTTTAAAAG 0.239 0.2410.298 0.898 1.017 46 22 16568261 5992801 8282 CAATATATTAYGCCAAAGTTG0.013 0.013 0.353 0.731 0.932 46 22 16574940 3788279 8283CTCTTTGAAGRTAGAGATTGT 0.405 0.405 0.509 1.031 1.075 46 22 165818051008378 8284 AGAACATCCTYGATGCTTACC 0.428 0.430 0.826 1.252 1.197 46 2216587611 8919 8285 TCTAGATGAARAAATGCAACT 1.436 1.374 1.471 0.982 0.96046 22 16593018 181382 8286 TGCAAAAACAYGTTCTCCAGA 0.935 0.936 1.785 1.1300.887 46 22 16598709 181396 8287 CTCTGCTTAARTGACAAGTCA 1.429 1.438 1.6231.403 0.988 46 22 16603741 5747339 8288 GAGGAAAAACYGTATGCAAAA 0.9870.961 1.290 1.573 0.736 46 22 16625446 2268786 8289CGGAGCAGCCRCCAGTGTGTC 0.042 0.059 1.065 1.341 2.107 46 22 166393855992838 8290 GCCCTCCCAGRGTCTGGCTGT 0.000 0.000 0.514 0.989 2.082 46 2216652682 426276 8291 TGCCACCTCCWGGGACTGGTG 0.000 0.000 0.020 0.591 2.10146 22 16665930 454799 8292 CTGAGTTTCAYTCTATGGGAA 0.000 0.000 0.092 2.0551.928 46 22 16678946 2305006 8293 TCGCTGAGTCKGCTCCCAGTT 0.093 0.0920.061 1.445 1.490 46 22 16690645 450796 8294 AGTGAGAGGCYGGGGGAAATC 0.0000.000 2.881 1.547 1.137 46 22 16703965 2111546 8295GCTGGATTGCMAAGCCGAAGT 0.161 0.163 1.776 1.337 0.809 46 22 167293375992128 8296 TCCTTCTTCAKGGAGCCCTGC 4.215 3.869 1.952 1.095 0.640 46 2216743102 2289718 8297 CGAACGCTGGRGTCTGTGACA 0.136 0.136 1.688 0.8820.710 46 22 16756240 5992895 8298 AAACTGTATCSGCAGTGACAC 0.228 0.2291.753 0.993 0.758 46 22 16769795 2083882 8299 ACCCATAAGCRTGAATTTGTT0.126 0.121 0.006 0.929 0.710 46 22 16782233 2401413 8300AAGCTTCCATSCCTGCTGGCA 0.086 0.088 0.028 1.006 0.589 46 22 167885294484121 8301 CCCTGCTGGGYACACACAGAT 0.016 0.017 0.049 0.073 0.745 46 2216808838 8135939 8302 TCGCCAAAGGRAAAACTCAGG 0.407 0.404 0.087 0.0881.067 46 22 16821385 445583 8303 GGCCTTCCCCRAAGGCAGACA 0.365 0.363 0.2720.062 1.170 46 22 16833681 365219 8304 AGCACTGCCCSATTTTCTTGG 0.394 0.3650.352 0.131 0.245 46 22 16860571 424765 8305 TTGGTAAAGAYGAATCCTGTC 0.7100.700 0.207 0.522 0.603 46 22 16873861 7284666 8306TTGGCAATCASTCTTCTATTT 0.285 0.273 0.328 0.649 1.020 46 22 16886836107321 8307 AACATGATTAYTGACTTTATG 0.019 0.020 0.924 0.709 1.086 46 2216897768 465101 8308 ATCTCAGCCTYTTTTGCAAGT 0.724 0.691 0.731 1.445 2.17646 22 16901343 462904 8309 TGACCGCTCGKGATGTTAAGC 1.575 1.681 0.882 2.1042.144 46 22 16920365 5992986 8310 CACATATGTTKGTCTTCAGAG 0.386 0.3792.175 1.789 1.828 46 22 16927573 1005195 8311 TGTGTAGCTAYGCATGCTCAT0.578 0.572 2.404 3.065 2.166 46 22 16937442 464541 8312CCCTCCAGATYGGATTCTTTC 1.752 1.733 1.418 2.755 1.906 46 22 16944707361818 8313 CGAGGTTTCCRTCACATAGCT 1.558 1.605 2.457 2.144 2.079 46 2216951892 361594 8314 GAAAACCGATYTGTATGGTCC 0.166 0.161 2.034 1.862 2.11746 22 16962268 361809 8315 ATGGGTGAATRTATGTAAGGT 2.159 2.143 1.292 1.6312.287 46 22 16971003 362043 8316 GCTCTGTGGAKTTGATAGGTG 0.045 0.044 1.0461.916 2.051 46 22 16979683 466456 8317 GACTGGGCACRAAGTATATGC 0.363 0.3561.095 1.488 1.503 46 22 16989428 2540620 8318 CTGACCTTATMTCCAATCCTC1.105 1.100 0.724 1.113 1.519 46 22 17003269 361534 8319TTTCTTGTACYGAATATTTAC 0.313 0.302 0.966 1.170 1.523 47 X 800307575959057 8320 TTTTTCTCCAWGTTTATTTAA 0.890 0.934 0.612 1.319 1.147 47 X80036134 5959059 8321 TCAAAGCAGAYGTTTAGCATC 0.497 0.503 1.550 1.2341.251 47 X 80050301 1166617 8322 CAAAAGCCTTYCGGCGTGGGC 0.721 0.727 1.4681.269 1.194 47 X 80058048 1166623 8323 CCAACAACCAKGACAGGCAAA 2.394 2.4711.487 1.445 1.178 47 X 80067200 1166634 8324 AGACACTCAGRTTTCAAAGAC 1.1651.183 1.607 1.415 1.031 47 X 80103135 1166676 8325 TTGCACATATYTATGAGGACA1.075 1.081 1.728 1.304 1.058 47 X 80134248 5913394 8326ACCCCCTGACSCTTCCTCAAT 0.522 0.520 1.074 1.375 1.215 47 X 80171239 — 8327ACTTCTGAACMGGAACATGTA 0.577 0.611 0.601 1.364 1.128 47 X 801917395959816 8328 GGAAGATTTAYACCAGGAACT 0.471 0.462 0.222 0.685 1.005 47 X80225545 5959831 8329 ATTTATTGTAYAGGCCTTCAA 0.088 0.085 0.155 0.5861.097 47 X 80235899 17333035 8330 TCCTTTTTATYTAATTTACAG 0.164 0.0810.140 0.255 1.074 47 X 80253754 5913411 8331 GTGATGCCCARTGTTCTGCTT 0.3330.319 0.260 0.146 1.552 47 X 80277451 12014025 8332ATTTCACTAGRCAGAAGTGTG 0.547 0.538 0.252 0.153 1.587 47 X 802970732027279 8333 GTTGAGTATCRGAAAAGTGAA 0.831 0.869 0.213 0.204 1.337 47 X80305924 17328569 8334 ATTTGGGACTRTGTTCATGCA 0.071 0.069 0.353 1.6121.229 47 X 80320875 5912483 8335 CTGCCTGGAGRATCTGTCTGA 0.078 0.075 0.4091.851 2.437 47 X 80325649 5959088 8336 CTGTGTGCATRCTGCCTGTGT 0.627 0.6481.946 1.817 2.156 47 X 80337509 2039717 8337 CGAGCAACTTRGAAAGACAAA 0.6130.611 2.115 1.592 2.354 47 X 80348471 4826212 8338 CTGTTTGATTRGATGACTCAT3.799 3.542 2.237 2.736 2.840 47 X 80359374 1044828 8339TTGTAATTTCYTATCGTAATT 0.960 0.961 2.029 2.483 2.840 47 X 803872755959099 8340 TTTTAATCTARCTCTTTATTC 0.167 0.173 3.004 2.576 3.024 47 X80400947 1117471 8341 GTCTTAATTTWTCCTACATTT 0.423 0.433 1.899 2.8583.133 47 X 80411297 5912496 8342 AAGGATGGATRTAACGGAGAG 4.215 4.422 2.2272.807 3.102 47 X 80417482 5913445 8343 TGTGGGGAATRGAAATTGAGA 0.478 0.4442.848 2.723 3.511 47 X 80426557 5913447 8344 ACGAGCATTARCTGTTTTATA 1.7531.742 3.159 3.397 3.415 47 X 80435314 6616745 8345 TTGAAGGACAYAGATGGATAC3.205 3.604 2.633 3.225 3.454 47 X 80452285 — 8346 GGAATATGCTRTTGTCCCTGC0.907 0.904 3.474 3.735 3.130 47 X 80459676 5959880 8347ATTGGAATTGRTCTTTGAAGA 2.992 2.874 3.447 3.214 3.392 47 X 80466557 — 8348GCTGTTATTTWTTTCCCTAGC 1.576 1.504 3.835 3.380 3.308 47 X 804787362806640 8349 TATTTGCAGAYATTTTTTTTT 2.412 2.255 3.441 3.348 3.237 47 X80492353 2602598 8350 GTAGTGTGCTRGATAACAGAG 4.015 4.091 3.280 3.2372.899 47 X 80506071 2444578 8351 CAGAACCAAARGGCTGACTAC 1.070 1.058 2.6772.903 2.903 47 X 80511331 2806658 8352 GATATACAAAKTCATCTTTAA 1.383 1.4172.574 2.668 2.860 47 X 80554116 10521395 8353 GTACCCTTAAYGGCCAGCTAA1.279 1.299 1.633 2.426 2.630 47 X 80583233 5912517 8354AGTACCAAATRTAAAGACATC 2.093 2.071 1.907 2.222 2.579 47 X 805970434529587 8355 ATGGGGAAGAYAGTCTCTATA 0.908 0.889 1.934 1.829 2.362 47 X80606131 5913519 8356 GATCGTTCACYTTTTGTGTGT 2.126 2.054 1.955 1.9282.183

TABLE 3 SNP markers found to be associated with Longevity in FineMapping studies in the QFP, Individual SNP markers genotyped in thegenome wide scan are presented in each row of the table. Thecorresponding region ID and chromosome is presented as identified inTable 1 (columns 1 and 2). The coordinate of the SNP according to theNCBI genome assembly build 35 is indicated in column 3. The RS# columncorresponds to the NCBI dbSNP identifier for the SNP (column 4). The SeqID is the unique numerical identifier for this SNP in the sequencelisting for this patent, and is indicated in column 5. Column 6, labeledFlanking Sequence, corresponds to 21 bp of nucleotide sequence centeredat the SNP, which is coded using the standard degenerate naming system.The remainder of the table lists −log10 p values for association of theindicated haplotype centered at the corresponding SNP with the diseaseas described in the text, using LDSTATS V4 and Single Type. Values forthe association of single markers, as well as 3, 5, 7, 9, 11 markerhaplotype windows are shown for LDSTATS V4 analysis (see text forexplanation of statistical calculations). The last two columns representthe −log10 P values for single marker genotype and allele associationusing Single Type analysis (see EXAMPLE section for description ofanalysis). Single Type LDSTATS v4 Single Genotype Single Allele RegionID Chr B35 Position RS# Seq ID Flanking Sequence Single Marker W03 W05W07 W09 W11 Likelihood Ratio Likelihood Ratio 1 1 173369115 107531338357 AATTAAGCAGRTTAATGCAGT 0.097 — — — — — 0.054 0.090 1 1 173391904726252 4850 CTGTCTCTCAYGCCTTCCTTG 0.299 0.007 — — — — 0.161 0.323 1 1173424493 1995651 4853 CCCAAAAAAAWTTCTATCATT 0.199 0.261 0.555 — — —0.092 0.225 1 1 173455634 1886766 8358 AGATTTGACCRTAAGCTCTGG 0.963 0.6150.680 0.351 — — 0.561 0.939 1 1 173475519 1325599 4858TTATTTCCCCRCATGAAAATG 1.615 1.183 0.530 — — — 1.660 1.658 1 1 17349168110913254 4860 TTTAAGCAGAMAGGCAAAAAC — — — — — — — — 1 1 17352215910732997 4863 ATTATTTGAARTAGATTTGAA 0.000 0.993 — — — — 0.006 0.004 1 1173539000 16850237 4865 AGATTAGAAGYGACCCTTGTT — — — — — — — — 1 1173564270 6425398 8359 CTCCAAGTCTYCTCTTGAATG 0.185 — — — — — 0.064 0.1962 1 225626459 — 4909 CACGCCTTTCYAGCCTCACAC — — — — — — — — 2 1 225645683342785 4911 AGGAGAAGAGMCTTCAAACTG — — — — — — — — 2 1 225660552 — 4912CACATCTACCRACCATCTGAC — — — — — — — — 2 1 225667166 — 4913AGTGGAGGTCRTTAGGAGAGG — — — — — — — — 2 1 225673850 342818 4914ACATTTAAATYATCCAGTCTG 0.221 — — — — — 0.137 0.228 2 1 225680359 75269494915 CCCATTGTTCRTTTTTGGAAT — — — — — — — — 2 1 225692972 16849638 4917TCATCTCTTAWGCAACTGAAC 1.515 0.568 — — — — 1.066 1.523 2 1 225703254 —4918 GCACATTTCAYCAAGCTGATA — — — — — — — — 2 1 225722322 — 4919TGAATATAATYATAAACTGAG — — — — — — — — 2 1 225741733 237778 8360TGAAAGCCCCRTCCTTATCAG 0.452 1.629 — — — — 0.193 0.463 2 1 225759122237819 8361 TCTATTGTTCYATACTGTCTC 0.194 — — — — — 0.084 0.203 3 1227250818 1202518 8362 GTCCTTGGGCRTGCGTTTTTA 0.214 — — — — — 0.108 0.2233 1 227258633 1202525 4971 TGAGGCTGCASGGAGGCCAGA 0.179 0.010 — — — —0.033 0.148 3 1 227265670 1202534 4972 GTTTTCCCCCRAAAAGGTTGG 0.143 0.1880.896 — — — 0.137 0.174 3 1 227270465 — 4973 ATGGCCCCGTRAGGTTAGCGG 0.5541.653 0.953 0.754 — — 0.347 0.571 3 1 227281064 6673201 4974AGGTGTCGCARAGATTAAGGC — — — — — — — — 3 1 227291324 16852841 4975TGCTCAGCTCYAAAAACTCCA 3.105 2.010 1.278 1.076 0.274 — 3.684 3.192 3 1227301328 1999903 4976 CTCTGAGGAAYGAAGACTTAG — — — — — — — — 3 1227307747 16852927 4977 ACCGGCTCCTYTCCTTTTGCT 0.910 2.235 1.429 0.465 —— 0.549 0.929 3 1 227315639 1202589 8363 TGCTTTAACCRAGTCAAACTG 0.0850.660 1.550 — — — 0.079 0.103 3 1 227320088 1202594 4978TTTTTCCAATKGTAGAGAGAG 0.051 0.527 — — — — 0.046 0.035 3 1 2273279151202603 8364 TTTCCTACCTKTTTCCTCTGT — — — — — — — — 3 1 227334767 67045274980 TTACTTCCTGKTAAGAGGTCT 1.087 — — — — — 0.690 1.089 4 1 2363002111110615 5016 TCTGAAAATCRCCATCTGTAA 0.604 — — — — — 0.314 0.647 4 1236311513 6429161 5017 TCTGCATAGAWATCTAATTCA — — — — — — — — 4 1236324113 477507 5018 CACCAACTAGRTTGAAAATTG — — — — — — — — 4 1236339319 613228 5019 TCCTTTTAATWTAAATATGTT — — — — — — — — 4 1236356440 682355 8365 CCAGACAGCCRTCTTCTTTAG 0.473 0.489 — — — — 0.2050.473 4 1 236365881 17598757 5020 ACTCTCTCTAYGTCTCTATAT 0.519 0.2700.224 — — — 0.376 0.533 4 1 236375371 672548 8366 TCTGTTAAAAYATTTAATACC0.520 0.423 0.444 0.318 — — 1.087 0.556 4 1 236381514 10495448 5022TTTTAATCCCRTAGAGCCAGA 0.049 0.078 0.514 0.295 — — 0.044 0.041 4 1236391685 10926012 5023 TCGTCTCAAARTAGTCAAGTA 0.067 0.149 0.012 — — —0.304 0.066 4 1 236403350 4659554 5024 AGGGGCTCACRTCTCAGTTGC 0.189 0.004— — — — 0.094 0.187 4 1 236410617 1953666 8367 CTAATCTCAASTGTTAGGAGG0.165 — — — — — 0.302 0.140 5 2 21157019 3791981 5075TGATCTCTCCRGAGCTATTGT 0.293 — — — — — 0.264 0.280 5 2 21162692 5708775076 CAAAATGTCTKGATTTCATTG 0.319 0.013 — — — — 0.449 0.309 5 2 211680391864423 8368 CATCTTCTTAKTACCTGGAAG — — — — — — — — 5 2 21171264 5203548369 TTGTGCAGAARAGAACAGAAG 0.175 0.068 0.068 — — — 0.071 0.203 5 221175552 1367117 8370 CTCTTTCAGGRTGCACTGGCT 0.181 0.172 0.134 0.028 — —0.133 0.187 5 2 21179434 512535 8371 CGTTTCCTTCYCTTCTAGGCA 0.410 0.2390.051 0.072 0.035 — 0.230 0.440 5 2 21185142 7575840 8372AGCCAGGAATKGTCAGTACTG 0.011 0.086 0.080 0.028 — — 0.005 0.020 5 221189574 6548010 8373 ACACATATTCRTAAGTTAAAC 0.056 0.001 0.021 — — —0.014 0.036 5 2 21194554 7590135 8374 CTCCAAGCCCRGCTGCTAAAA 0.049 0.035— — — — 0.014 0.036 5 2 21199203 — 5080 GCCGTCCATCYATCCGTTACT — — — — —— — — 5 2 21204658 594677 5081 ACTGAGTAATYGTCTAATGAA 0.248 — — — — —0.222 0.263 6 2 49991254 10495984 5116 AATGATTTATSTGTTCCTATG — — — — — —— — 6 2 50004404 17490406 5118 GTTTATGTGAYTGTGACATTC 2.251 — — — — —1.910 2.269 6 2 50010410 1981797 5119 AATTTTTCTCYTTAATATATC 0.754 1.531— — — — 1.024 0.720 6 2 50029185 12998574 8375 AGTTAACAAARGGGTTTATGT2.157 1.822 1.063 — — — 2.915 2.113 6 2 50037491 1156742 5120AATGGTATAARAATATTAGGG 2.751 1.768 1.604 1.406 — — 2.900 2.666 6 250048374 17039425 5121 CTATTTGCCTRTGATTTTTAA — — — — — — — — 6 250055972 12465974 5122 ATCAAAGATTYCAATTCTTGG 2.415 2.041 1.806 1.2571.048 — 1.820 2.414 6 2 50064931 10495987 5123 TTGGAAGCTAMTGATTATTCT3.062 2.226 2.193 1.645 0.992 — 2.424 3.061 6 2 50077180 6758043 5124ATCATTGCTTRTGTAATCTGA 0.891 2.615 1.518 1.155 — — 1.181 0.893 6 250087864 10166360 8376 AATAAAGGCTSTAAAACTTAA 0.060 0.770 1.283 — — —1.485 0.048 6 2 50095750 6713560 5127 AGCTTTTAACRGTTCTCAAAG 1.394 0.601— — — — 1.237 1.392 6 2 50101389 17039577 8377 TGACCTTTCAYATTTCCCATG1.391 — — — — — 1.438 1.378 7 2 51025393 10490172 5237TTGCTGCTTASAAACAAATTC 0.100 — — — — — 0.118 0.105 7 2 51032119 170410915238 GGACAGACAAKTATTTTGTAG — — — — — — — — 7 2 51039440 17041095 5239TAGGGCTCTTMAATAGTAGGA 1.289 1.369 — — — — 0.865 1.314 7 2 510469921160595 5240 TGTTTCATGTYTTAAAGTGGG 1.748 0.763 0.579 — — — 1.595 1.789 72 51052993 1003017 5241 TGGACATACAMTAATGTATCT 1.419 1.044 0.219 0.880 —— 1.404 1.462 7 2 51058686 741421 5242 CTGTCATTCAKTCTAACTTGC — — — — — —— — 7 2 51076203 1541602 5244 CTATCAGTAGYATGGGAATGA 0.990 0.593 0.6340.307 0.654 — 0.746 1.047 7 2 51083805 7579976 5245CATTCTAACAYCTACAAAGAG 0.741 0.654 0.450 0.554 — — 0.834 0.786 7 251096961 10205578 5247 TGGAAAGATTKTAATCAGAAA 0.979 0.530 0.319 — — —0.817 1.020 7 2 51107252 10174398 5249 TCTGTACCCAYGCTCTCTCCA 0.723 0.577— — — — 0.577 0.764 7 2 51126792 17041161 5251 AGAAAATCCAYAAGTCTAAAT — —— — — — — — 7 2 51140357 10195460 8378 TTAAGCATACRTTTTTCCACC 0.999 — — —— — 1.018 1.048 8 2 121763582 2580344 8379 TATTTCTCCCYCAAGTTCCAG 1.115 —— — — — 0.740 1.142 8 2 121795575 2713226 8380 ACGAGCAGGTKGTCACCCACC0.843 0.519 — — — — 0.495 0.821 8 2 121809417 2713250 8381GGAGCACTGCYGGACCAAATG 0.597 0.556 0.452 — — — 0.328 0.596 8 2 12183396812479320 5309 TGGTGCCAATRTCTCTAAAAG 1.026 0.669 0.408 0.176 — — 0.6221.064 8 2 121870433 10496566 5312 ATATAGAATAWGTTTCAGCCC 0.994 0.6810.499 0.416 0.129 — 0.646 1.101 8 2 121886006 10191223 5313ACCACAGAAAKGGAGAAAAAT 0.162 0.691 1.152 0.591 0.336 0.122 0.136 0.186 82 121905688 13032411 8382 AATATTTTATKTGAAAAGTCA 0.187 1.531 0.876 0.8210.634 — 0.136 0.186 8 2 121923235 1975305 8383 CATCTAAGACYATACCTAGAA1.780 0.871 0.792 0.830 — — 1.309 1.866 8 2 121940587 7587659 8384CATGTTGCTTKTAAGAACAAA 0.652 0.928 0.846 — — — 1.196 0.662 8 2 1219821546704587 8385 GAGCAACTGCRAATAATTCCC 0.493 0.429 — — — — 0.214 0.480 8 2121995577 10221922 8386 CACACTGGCTKTAGGGAAATG — — — — — — — — 8 2122017375 2164797 5325 AGCCCCCAGCSCGCCAACAGG 0.423 — — — — — 0.212 0.4099 2 127668493 13006847 5367 GCTGAACAGGYCAAACTATTT 0.844 — — — — — 0.4440.818 9 2 127692604 6710496 5368 AAGCCCCCATMCCAGAAATAA 1.163 0.474 — — —— 0.887 1.149 9 2 127723916 6430936 5369 CCCGAACAGGMTTTTGTTCAC 1.1740.407 0.234 — — — 0.873 1.188 9 2 127729734 4662717 5370GTTCCTGGGGYTTGCACTGAT 1.263 1.012 0.831 0.313 — — 0.825 1.298 9 2127749557 4150474 5371 ACAAACCCACMAAGAAAACAG 1.562 1.586 1.193 0.684 — —1.194 1.572 9 2 127754774 4150454 5372 CCATCACTTTYAGACCTGTCC 2.194 1.6191.471 — — — 1.661 2.272 9 2 127776393 4233584 5373 CCTGGATTTCYTACTCACTGT— — — — — — — — 9 2 127802584 12613413 5374 TTTTGATGGTYCACATGCCAA 0.1222.011 — — — — 0.150 0.133 9 2 127828431 6714840 5376GCAAAGGATCWGTTTCCAAGT — — — — — — — — 9 2 127853791 4662724 8387GCCTAGAACAYAGAAAATTAC 0.112 — — — — — 0.150 0.133 10 2 162628815 19138078388 GAAAAAATAAYCCATGGAGAA 0.084 — — — — — 0.050 0.118 10 2 1626441284500960 5442 TCCAGCAGCAYGTTACTGTCT 1.285 1.337 — — — — 0.878 1.321 10 2162653508 4664442 5443 CTTTTAATGGRTCCTATGTAA 1.592 2.139 1.964 — — —1.104 1.601 10 2 162657263 4295021 5444 CCTGTTGATTKTTTAGCTGAA 2.4022.135 1.590 2.753 — — 1.935 2.486 10 2 162671072 1861979 5445TTAAAGCCTGYAAGCACCAAA 2.620 1.464 2.590 3.182 2.397 — 2.079 2.610 10 2162680415 2287509 5446 CCTCCTTGTTKCTCTCCAAAT 2.467 2.729 3.236 2.7861.484 — 2.171 2.527 10 2 162694710 1014445 5448 TCTGTAAAGCRCTCTCATTTC2.677 4.279 3.467 1.629 — — 2.160 2.676 10 2 162709469 2300755 8389CCCCTGTCCTRTATCAGTGGT 3.325 2.591 2.307 — — — 2.922 3.347 10 2 16272027310930040 8390 TAACTATTTTRAAAGCTTATA 2.463 1.679 — — — — 2.034 2.509 10 2162749424 12469968 5453 CTCTACCTCARTTATACATCC 0.898 — — — — — 0.5680.914 10 2 162762494 7593348 8391 TCTCACTGGGKTTTGCCAGAG — — — — — — — —12 3 7122158 17046783 5601 TCATGAATTTYGGCATTTTTT — — — — — — — — 12 37128652 6778030 5602 TTGACTATCTRTGAAAACTGT 0.125 — — — — — 0.057 0.14312 3 7136316 17234935 5603 TCATAATCTASGACTGGATAT 0.774 2.621 — — — —0.421 0.782 12 3 7148002 1878164 5605 ATGACACTTCRTCTACTTGAA 0.757 1.7272.848 — — — 0.447 0.792 12 3 7155162 6804466 5606 ACTTTATAGGYATACTGGTAG0.765 1.857 1.801 2.675 — — 0.511 0.811 12 3 7162872 17234969 5607ACAACCATGAYCCTGACCTTG 2.545 1.710 1.752 1.496 2.027 — 1.960 2.598 12 37168821 7623514 5608 TCCTCAAGAAMGACTTGCATT 0.188 1.610 1.593 1.407 1.277— 0.106 0.204 12 3 7174495 6443093 5609 AATGTAGAAGYGGCAGAAGAC 0.4170.298 1.135 1.219 — — 0.287 0.422 12 3 7187512 17235018 5610AAACCTACATSATTCTGTATG — — — — — — — — 12 3 7193175 1400166 5611TCATCCTTTCRTCTTTATTCA 0.291 0.206 0.184 — — — 0.116 0.335 12 3 719873913082571 5612 GGCTAAAGAAYAGTACAAACC 0.000 0.356 — — — — 0.035 0.000 12 37204619 11708019 5613 CAGTTGTCTCRATGCCTAGTA 0.000 — — — — — 0.035 0.00013 3 33009602 4075736 5725 TTTCATAAAGRAGAGAAATAA 0.437 — — — — — 0.4010.442 13 3 33046631 6762132 5728 CAGGAGGTATRAACAGTGCTG 1.158 0.828 — — —— 0.765 1.173 13 3 33062204 6780220 5729 CTGCCTTAATMGGGGCTATAG 1.0792.419 1.095 — — — 0.916 1.093 13 3 33074192 4438612 5730GTGTCCTGCCRAGGTGGGAGG 2.594 1.997 1.144 1.432 — — 2.137 2.651 13 333104989 9858362 8392 TTCTCTATACMCTAAAAATGA 1.953 1.070 1.537 1.3161.836 — 1.419 1.923 13 3 33121193 4578976 5732 TCCAGAGATAYGAGTTGGGAC0.535 0.770 0.365 1.623 1.983 0.880 0.279 0.582 13 3 33133916 46786865733 TGCAGCCTCARGTGCATCATA 1.299 0.612 1.108 1.210 1.924 — 0.930 1.30813 3 33151828 7652193 5734 ACTGTGGTTTRCAAAGTATAG 0.859 1.157 1.526 1.003— — 0.583 0.891 13 3 33179004 4678490 5735 TTCTTTGAACWGACGAGTAAG 0.0491.195 0.992 — — — 0.101 0.051 13 3 33191738 4465894 5736CTGGCAGAATRCCTGGAATAG 0.576 0.168 — — — — 0.481 0.601 13 3 332173464678763 8393 TTATGGAAGGYAGAACTTGCA 0.123 — — — — — 0.198 0.150 14 340246701 1317217 5803 ATGATTAATAKCTGGAGAAAG 2.772 — — — — — 2.185 2.83714 3 40284229 9861194 8394 CTGATTCAATRTGTAAAGGGC 3.148 1.703 — — — —2.337 3.157 14 3 40314657 4974047 8395 TCGAATGGTAKGCTTAACCTC 3.104 1.4671.179 — — — 3.310 3.035 14 3 40349555 4974067 5811 AAGTCTAACAYGCAGCTCTGA3.097 2.030 1.870 1.540 — — 2.697 3.130 14 3 40379774 10510708 5815TGAATCAAGTYGGTCTACAAT 2.631 2.530 2.299 1.508 1.147 — 2.493 2.661 14 340398626 9854493 5816 CTCTAGTCTAYAGTTAGCTAT 3.587 3.052 1.764 1.819 — —3.110 3.624 14 3 40404187 2305521 5817 ATGTTCTGAARAAAAGCCCCG 2.481 2.4712.174 — — — 2.255 2.496 14 3 40417584 — 5818 CTGGAGAGGTSCTGAGATGTT — — —— — — — — 14 3 40424355 6783755 5819 TCTAAGAAGTYAGACAGACAT — — — — — — —— 14 3 40431030 7645864 5820 GCAAAAGGGAYTGTGGTGTAC — — — — — — — — 14 340468222 6801859 8396 AAAAGATCTAKAACTTCCCCA 2.353 2.675 — — — — 1.9642.381 14 3 40496977 4571217 8397 AATCTTTACARTGATGACTGC 3.418 — — — — —3.016 3.499 15 4 77256496 10031733 8398 TATGACCTTAYGAGGCATTCT 0.078 — —— — — 0.071 0.073 15 4 77291919 867562 5870 CTGTGTATTTRAGTTGTTTCC 1.0840.594 — — — — 0.648 1.038 15 4 77320175 7684461 8399GAGAGGCAGAKAGATAACTGT 0.130 0.914 1.015 — — — 0.098 0.155 15 4 773526424304003 5874 GACTCCTGACRAGGTACCTGA 0.091 1.438 1.131 0.923 — — 0.0980.102 15 4 77384429 10028141 5878 AAATATTATTRACACTTCAGA — — — — — — — —15 4 77402183 11733489 5880 TATATCTACGRTGATGCCTCT 1.838 1.256 1.2701.441 — — 1.441 1.949 15 4 77430723 6824251 5882 CACATTCCAAMATAACAGAAC1.919 0.907 1.838 — — — 1.443 1.932 15 4 77456563 17001573 5885TTATTTGGGCRTGTAGCTATG 0.016 1.718 — — — — 0.006 0.033 15 4 7748824817001659 5889 GCCCTGACTGYGGCCAGTTCT 1.662 — — — — — 1.130 1.605 15 477516879 1441922 8400 TCAAGAAGTARGCAGGTAAAG — — — — — — — — 15 477550724 1441911 5893 AGATTTAAGTKCCTAAGTTGG — — — — — — — — 16 4101082500 10031644 5916 AGTAGGGAATKTATTTGCACA 0.147 — — — — — 0.6190.124 16 4 101095575 2282588 5917 GAAAACAAGTWGGTTGAGAAA 2.159 0.939 — —— — 1.688 2.164 16 4 101103675 10031708 5918 CAGAGCTTCTYGCATCATTTC 0.0001.703 1.067 — — — 0.103 0.029 16 4 101110330 2162386 5919AACAGGTTATMTTTCAAGGCC — — — — — — — — 16 4 101123160 4699387 5921TTTGACGACASCTACCGCTAT 0.550 0.270 1.681 1.127 — — 0.263 0.563 16 4101129781 4699767 5922 GCACCTTATCYGGGCCCAAGT 0.551 0.359 0.371 1.3631.263 — 0.263 0.563 16 4 101130928 11935615 5923 CAGCTTGTTCRGGTCATGTTC0.445 0.575 0.662 1.549 1.591 1.034 0.234 0.443 16 4 101149661 68299125924 GCTGAGCCTAYGGTCTGCTCT 0.515 0.743 1.170 0.967 1.402 1.660 0.3280.567 16 4 101158451 11723286 5925 TTTATTTACARAGGCTATTTT 0.842 1.0021.180 0.783 1.190 — 0.510 0.831 16 4 101183482 17029641 5928TCTTACACATRAAGTATATGG 1.318 1.174 0.826 1.559 — — 1.385 1.299 16 4101209057 17613664 5930 AGTGTGTTCTYCAAAAAGATT 0.064 0.986 1.268 — — —0.391 0.067 16 4 101233826 11736218 8401 GCTAAGAAGAYTAAACAGGGA 0.6581.841 — — — — 0.359 0.661 10 4 101258022 7689566 5933AGAGCTATCARTTTATAGAGC 2.484 — — — — — 1.955 2.505 17 4 126670525 15092928402 GTTCCCACACMAAAACTAGGA — — — — — — — — 17 4 126681071 12501179 8403TATTTTCATGRGTCTGAATTG 1.184 — — — — — 0.960 1.200 17 4 126691002 46230228404 TGGCCAAAGTYACTTTTTAGA 0.380 0.459 — — — — 0.523 0.388 17 4126705065 6842220 5973 TAATGAAAATYGCTTGAAAAC 0.364 0.347 0.737 — — —0.472 0.365 17 4 126717061 1395241 8405 TTTCTAGTGCRTGTTAAAGAT 0.9850.833 0.815 0.894 — — 0.861 1.012 17 4 126726483 13108706 5976CCTTATGGCARCTTTTCTGCA — — — — — — — — 17 4 126738202 17009708 5978GAAAGTCTGCRGATACCATGG — — — — — — — — 17 4 126752525 12506486 5980GGACATGGGAKCAAGAGGTGA 1.623 0.944 1.353 0.549 0.213 — 1.218 1.693 17 4126766922 7686537 8406 TGAAAGTGTAYGAAAGGTTAA 0.461 1.111 0.697 0.435 — —0.460 0.465 17 4 126781219 1395228 8407 TTTTCCATTAMTACCTAACAC 1.6390.832 0.746 — — — 1.113 1.642 17 4 126796200 10034622 8408TCATACAACARGTGTGTGTTT 0.814 1.120 — — — — 0.569 0.785 17 4 12680472017009923 5988 TGGGGAAAAGRGATTAAAAAT 0.372 — — — — — 0.143 0.391 18 4143639478 1497397 6062 ATAATAGTCAMGATGATGGTT 0.283 — — — — — 0.194 0.30718 4 143657608 6828156 8409 GAGGTCCCACKACCCTTGTTG 0.736 0.184 — — — —0.443 0.790 18 4 143679095 1497401 8410 ATTTAAACTAYCTTAAAACAC 0.0590.063 0.149 — — — 0.253 0.055 18 4 143700188 3775711 8411TCTAGCACAGYCCCAGAGGCA 0.425 0.640 0.786 0.617 — — 0.203 0.485 18 4143718806 931637 6070 CTGTCAGAACRTAAATTTGAT 2.137 1.465 1.318 0.9240.654 — 1.832 2.178 18 4 143739539 17016163 6071 GATGCCCTTTSCAACACATAT2.923 2.092 1.419 1.495 — — 2.319 2.946 18 4 143764438 3844178 6073TTAAGAAATARGGAACTGAAC 3.342 2.535 1.754 — — — 2.797 3.350 18 4 1437854792322795 8412 GAGCTAGAAASATGGGGAGAT 3.352 2.057 — — — — 2.875 3.369 18 4143808180 975136 8413 GCAGATAAGAYAATTCAGGTA 0.194 — — — — — 0.232 0.20019 6 27117491 7775041 6096 TTGACTATATWCATGCATTCT 0.400 — — — — — 0.1790.416 19 6 27131673 10946899 8414 GGAGTAAAATWACTTTCCTAA 0.809 0.603 — —— — 0.760 0.865 19 6 27138903 3922717 8415 CTTTATATCTRTGCAAGAACA 1.2510.931 1.457 — — — 1.325 1.311 19 6 27150237 6916301 8416AATCCTTCTAYTTTCTAGTCC 0.015 1.108 1.332 1.013 — — 0.142 0.041 19 627155692 994691 8417 GTATAAAGAAWGCCTTGAGAA 1.927 1.469 1.051 1.086 0.969— 1.416 1.965 19 6 27162476 2142685 6098 TTTATGTTCTYTTGAAGATGA 0.8751.410 1.048 0.977 0.924 0.929 0.510 0.902 19 6 27172468 — 6099GTATTCACTCRAGATGTTAGT — — — — — — — — 19 6 27183462 9348752 6100AGAATGTTCAYAGATATTTCT 0.594 0.764 1.718 1.106 0.762 — 0.330 0.629 19 627193204 12209456 8418 CTAAGTCATGYCCAGACTCCT 0.963 1.028 1.272 1.222 — —0.603 1.016 19 6 27201800 6456768 6101 TGGAGGATGGSATAGTAAAGA 1.332 0.8150.662 — — — 0.890 1.372 19 6 27211559 2022272 6102 ACAATTAAATRTACAGTGGTT1.161 0.668 — — — — 0.793 1.241 19 6 27222031 9393790 6103TACTCAGTGTKATAAGATCAT 1.039 — — — — — 0.649 1.058 20 6 152767168 69053396172 AACAATGTGAYACAAACTGTA 0.984 — — — — — 0.963 1.023 20 6 152789820214955 6175 GAAAAGGTGAYGCAAAAATTT 1.050 1.247 — — — — 0.662 1.093 20 6152810619 549981 6176 TCCTCAGGCCYTTTCTTTTCC 2.519 1.758 1.509 — — —1.935 2.545 20 6 152838247 6909684 6179 TATATTAAAAYGAAATGGACT 2.0792.393 0.958 0.811 — — 1.750 2.121 20 6 152861412 17082700 6182GTATCCCATAYAGCAAGAAGT 0.217 1.413 1.564 0.969 1.005 — 0.131 0.207 20 6152868292 214970 6183 GTGACACAGTRTCTATACTAT 1.682 1.192 1.408 1.5060.629 0.721 1.188 1.732 20 6 152875047 579464 6184 CTTAACTTCCRGATTCCACAG1.683 1.719 1.232 0.782 0.964 0.559 1.188 1.732 20 6 152887970 77554376185 ATTCATAATTMCTTATATTGA 1.696 1.534 0.964 0.668 0.906 — 1.188 1.73220 6 152898003 9397512 6186 TGTTGCCATAYCACTGTGTAG 1.600 1.007 0.8421.625 — — 1.092 1.634 20 6 152913124 9479327 6187 AATTTATTTTYTGGATGATTA— — — — — — — — 20 6 152925152 11155857 6188 CCAACAAAATRGACTTACATG 0.4980.856 1.639 — — — 0.511 0.532 20 6 152934088 17699371 6189CTGTTAGACAYGACACAGTCT 0.051 0.435 — — — — 0.476 0.072 20 6 15294082811155858 6190 AGCCATATACRGTCATTAAAA 0.797 — — — — — 0.479 0.845 21 730540960 255160 6249 ATGAAAGCTGYCTTGCCTAAG — — — — — — — — 21 7 305650884723010 6251 CTACAGCTTCRATGGCAGCCC — — — — — — — — 21 7 30579153 39018486252 TGTCCTTCCARTGTAAAGTCC 0.985 — — — — — 0.591 0.977 21 7 3060227911768076 6254 TGTGGCCTTTRAGTACCTTGG 0.000 1.691 — — — — 0.492 0.007 21 730610787 7803974 6255 AGCAGGGCTAYGTTCTGAGAA 1.896 2.000 1.618 — — —1.478 1.861 21 7 30618224 1990011 6256 TGGATATGTCSAGTGGAGCTT 0.000 2.4931.570 0.754 — — 0.612 0.009 21 7 30629046 10237363 6257CCCGGGTCTCRTATGCCAAAT 2.753 2.182 1.118 1.180 2.561 — 2.281 2.819 21 730641902 17159487 6258 GTAAGTTAACKAAGGTCTCCA 1.232 1.492 1.742 2.3832.564 — 0.814 1.222 21 7 30649882 10229281 6259 TGGGAATAGARCTGCTTCATC0.450 1.687 2.864 3.310 — — 0.399 0.463 21 7 30671937 17159526 6260TCAGGTTGTTSCAATGTCCCA 3.299 3.169 2.385 — — — 2.786 3.305 21 7 3068286617159567 6261 CAAAGTTATAMATCTCTTAAA — — — — — — — — 21 7 3068850310216063 6262 GCATTACAACRTGTGCCAGGT 3.574 3.298 — — — — 2.875 3.586 21 730710418 1000597 6264 AGGACAGTTTYGTAATCAGGA 0.322 — — — — — 0.127 0.34522 7 33466478 7811071 6364 CAGCATTTGTRAGGCCAGGTG 0.368 — — — — — 0.2690.376 22 7 33474415 2034513 8419 CCATGATTAAYGTACAACATA 0.206 0.483 — — —— 0.140 0.218 22 7 33478828 7796551 8420 TTTGAAATATYTAATTGATGA — — — — —— — — 22 7 33484293 4723309 6366 TATCAGTTTTRTAAGTAAGCA 0.000 0.243 0.364— — — 0.091 0.014 22 7 33491746 6966497 6367 CTTGTGGTGAYCAGCACTACT 0.0000.670 0.275 0.345 — — 0.082 0.009 22 7 33501439 1545449 8421TTTTTCTTCGYAAGTTCTTAA 0.285 0.638 0.639 0.175 0.082 — 0.369 0.299 22 733505602 1376350 6369 AACTGAGAGCRATTGTGGCAA — — — — — — — — 22 733511585 921413 8422 TCTGACAGAARAATGTGGGGT 0.468 0.548 0.423 0.294 0.055— 0.305 0.503 22 7 33516650 7796839 8423 CTAGTGCAATYTCATTTTGAC 0.5090.181 0.401 0.168 — — 0.341 0.494 22 7 33524601 17810887 8424CTGCAAAGCTRGGAACCAGGG 0.365 0.380 0.216 — — — 0.225 0.384 22 7 335302356943676 8425 GTTGCTGAGGSACTTTACTCC 0.790 0.439 — — — — 0.880 0.812 22 733537029 3779238 8426 GCCATTGCTAYGATTTCTCCC 1.052 — — — — — 0.803 1.07423 7 127442525 2060736 6407 TAACCGCGCCSTTCATTCTGT 0.393 — — — — — 0.4460.386 23 7 127446016 11981584 8427 GAAGTTGCCARCCCCACATCA 0.261 0.039 — —— — 0.116 0.303 23 7 127451842 2167289 8428 GTGGTATCCTKACATGCTGTA 0.6080.878 0.619 — — — 0.365 0.641 23 7 127456753 791595 8429GGGCAGCTGTRGCCACAGAAT 1.522 1.537 0.728 0.868 — — 1.143 1.594 23 7127459767 791600 8430 ATGCCAACTCRGGCCACATGC — — — — — — — — 23 7127464579 1376349 6409 TGGTTTAAGCYTTTGAGCTCT 2.033 1.803 1.812 0.4150.688 — 1.709 2.125 23 7 127469340 4731424 8431 CCCAGGGGCTKGCATTCTCGG1.638 1.273 1.175 1.213 0.752 — 1.411 1.725 23 7 127475802 2278815 8432AATGAGAGGGRCTGTGTAAGG 0.147 0.538 0.470 1.698 — — 0.034 0.155 23 7127478340 4731427 8433 GAGATCAGATYTTTCTGATGA — — — — — — — — 23 7127484013 11763517 6411 GTGGCCATTAYTTGAGAGTGA 0.683 0.487 0.325 — — —0.392 0.692 23 7 127488256 3828942 8434 TCACCTGGGTRCAGGATACAA 0.0840.501 — — — — 0.018 0.106 23 7 127491020 11761556 8435GACAGGAGGGMAAGGGCCATG 0.388 — — — — — 0.196 0.389 24 7 147429773 96485238436 AGAAATCACAMTTTTTGCAAA 0.033 — — — — — 0.023 0.019 24 7 14743310813244714 8437 AGGGTTGAAARTGCTTCTAGG 0.421 1.613 — — — — 0.740 0.419 24 7147437045 4726947 6676 TTTAGATGTASATTAAGACCC 1.295 1.015 1.009 — — —0.946 1.311 24 7 147440758 2373341 8438 AAGAATGAACRGGAATCACAT — — — — —— — — 24 7 147450906 11514853 8439 AATCTACATARAGCAACTGGG — — — — — — — —24 7 147453963 4726950 8440 CTGTAATTAARTACTTTTCCC 0.614 0.580 0.9530.926 — — 0.564 0.614 24 7 147458148 1918298 8441 CGTATTTTGTKCATGATTCTA0.091 0.603 0.410 1.651 1.669 — 0.365 0.107 24 7 147461850 1918296 8442CAGATTACACRAAGGAGTGAA 0.180 0.686 2.503 0.985 1.230 1.922 0.049 0.196 247 147468476 7779793 8443 AGATTCACTGYAGTCAGAAGA 0.425 1.819 2.190 1.8870.986 0.934 0.266 0.489 24 7 147473270 10281587 6678CTATCTCTTAYATTGCCCAGT — — — — — — — — 24 7 147478836 11767934 6679TTGTCAGATGSTCTTCACACA 2.329 1.847 1.343 1.667 1.114 0.935 1.776 2.367 247 147485200 2037869 6680 TGAGGTCATGYGTAGTAAACA 0.740 1.733 1.341 0.8421.698 — 2.078 0.762 24 7 147491445 — 6681 TTTTAGAAGTRTGCTCATTTA — — — —— — — — 24 7 147497490 1177942 8444 AAACAGTCTAYGCTTTGGCAA 0.845 0.7251.064 1.361 — — 2.150 0.867 24 7 147502534 1637864 6682ACATGTCACCRAGAGAAAATG 0.000 0.665 0.736 — — — 1.080 0.012 24 7 1475084981730397 8445 GGCTATTACCYCAATCTGAAA 0.000 0.000 — — — — 1.080 0.012 24 7147514126 1637854 8446 AAAAGAAGAARTCTCCTTTGA 0.000 — — — — — 1.080 0.01225 7 149524163 4725887 8447 TGGCAGCGTGRTGTGTGGACA 2.923 — — — — — 2.8072.975 25 7 149556724 2888635 6721 TGCATCGATCKGGTGAGACAC 1.117 1.686 — —— — 1.374 1.163 25 7 149591461 7810209 6723 TTATAGTGGGYGATGTGTAAA 0.4980.540 1.212 — — — 0.214 0.463 25 7 149624893 2222524 6725ACAATGGGTGRCTTTATTTAT — — — — — — — — 25 7 149649666 7810752 6726TGAAAATTCARCTTGTTCAAG — — — — — — — — 25 7 149690467 4725919 8448GGATCCCACTYGTTCTTCCCC 0.228 0.978 0.568 0.724 — — 0.082 0.265 25 7149706735 13242186 6728 ATCTCTGAGTSCAGCAGTAAC 1.949 1.195 0.652 0.5070.526 — 2.052 1.980 25 7 149738096 4367453 6730 TTAAAAAGGTYATTCCTACCC —— — — — — — — 25 7 149749542 — 6731 TCTGTTTTTCYCCAGCAGAAG 0.000 1.1250.897 0.791 — — 0.848 0.006 25 7 149756449 10253121 6732ATGTGACAGTYTCTAGTTCCC 0.713 0.457 1.178 — — — 1.000 0.732 25 7 14976383910272462 6733 AGGAATTTGARTGAATGGGCA 0.734 1.318 — — — — 1.000 0.732 25 7149768593 6946579 6734 TTGCCTAAGTRTTTAGAAAGT 0.683 — — — — — 0.400 0.69926 7 150246812 6951528 6762 CCTGGGCTGAMCCAATGAGCA 1.798 — — — — — 3.6171.856 26 7 150260307 6464131 6763 GGTGCCCGCTSCTTCCCGTTC — — — — — — — —26 7 150269456 6979622 6764 TGTGCCCTAAYGCAGCCCTCG — — — — — — — — 26 7150286812 6464132 6765 GCTTCGTGAARGTAGGTATTC 1.388 0.812 — — — — 0.8831.380 26 7 150295919 4725395 6766 CTTCTTTCCARTTAAAATATT 0.817 0.7640.577 — — — 0.534 0.820 26 7 150312660 4726005 6767ACACAGAAGTYATCACTCACA 2.171 0.995 0.638 0.657 — — 1.705 2.196 26 7150320869 2257069 6768 GCAATTTCCCRATGCGGGCCA — — — — — — — — 26 7150339257 310583 8449 GTGAGCTTGARATCCTGCAGC — — — — — — — — 26 7150348102 2608288 6769 AGTAAAGGGASAAAGTTCTTC 0.305 0.847 0.942 — — —0.116 0.319 26 7 150355235 7784344 6770 GGCTATATTAWAAACTAATAC 0.2920.090 — — — — 1.019 0.294 26 7 150373700 1870238 6772AATGACCACASGGCTGCTGTC 0.419 — — — — — 0.198 0.412 28 9 26960384 78707878450 ATGACTTTGGWCTGTTCCAAA 0.378 — — — — — 0.166 0.413 28 9 269690277849248 8451 CAGAGTTTTTYGTTTTCTGTA 0.377 0.421 — — — — 0.166 0.413 28 926986170 7018585 8452 GTTTGGAAGASCAATATTTTG 0.093 0.628 0.507 — — —0.040 0.090 28 9 26996575 10967657 8453 AGTCTGGGTGRCAAGTGGTAC 0.3810.382 0.483 1.087 — — 0.173 0.417 28 9 27003594 7040505 8454GTTTTAGCTAKTTTGATAGGT 0.011 0.595 0.939 1.176 — — 0.075 0.033 28 927008020 12337896 6914 AATCATTTCARAAAGGTATTA 0.019 1.648 1.221 — — —0.061 0.017 28 9 27018947 7390085 6915 TGGAAAAGATRTCTAACCTCC 2.317 1.548— — — — 1.828 2.352 28 9 27026804 10967677 8455 GAAAAGTGGCRTGTGATTTAC —— — — — — — — 28 9 27031315 7045747 6916 ACATTCCTGTYAGAGCACTTA 0.017 — —— — — 0.001 0.024 28 9 27037246 10812520 8456 TCAGCAGTAAYCTCTCTTATG — —— — — — — — 29 9 70416653 10435958 8457 AAATTCCTTAWGTTCAAATGT 0.424 — —— — — 0.349 0.449 29 9 70433985 7865858 8458 AGCACATTTARTCTCTGAGGA 0.7620.072 — — — — 1.065 0.810 29 9 70450716 7862783 8459GAGGCCCAATRAATGCTGGCT — — — — — — — — 29 9 70470740 7024154 8460CTGAGTGCATMGTGAATTCCA 0.030 0.207 0.358 — — — 0.009 0.036 29 9 704875224297092 8461 TACTTGGAGTWAATTTATTAG 0.061 1.364 0.713 0.264 — — 0.0370.080 29 9 70497790 11142532 6979 ATGGAGAGAAYAGAGGAATGT 2.303 1.5580.588 0.351 — — 1.695 2.337 29 9 70509554 11142536 6980TGGCTGACAGYGCCTCAGAAA 0.790 1.049 0.668 — — — 0.431 0.799 29 9 7052980910868873 8462 ATTCCTCTCTMTGTTTCACTA — — — — — — — — 29 9 7054532611142556 8463 AATGTTAAACRTGCTAAATAT 0.738 0.170 — — — — 0.451 0.765 29 970587221 11142561 8464 GTAACAGTTGYCCTGCCTACC 0.813 — — — — — 0.506 0.86730 9 93389779 10119086 8465 CTAAACACTAWCTCTGTTATA 1.784 — — — — — 1.4351.783 30 9 93410054 6479494 8466 CCAACTTAGCRTCATTTGATG 0.071 1.213 — — —— 0.151 0.070 30 9 93432674 2150752 8467 TGTGGTGGTGKTTTCTGACTC 2.5660.990 0.939 — — — 1.984 2.574 30 9 93459352 12551314 7054TCCCAAGAGGMAATTGTGGGG 0.000 2.013 0.720 0.435 — — 0.000 0.016 30 993475886 1806458 7055 GGTGTTCCTCRCAGGTGGTGA — — — — — — — — 30 993485419 6479499 7056 AGCCCTGTAGWTCCTTGAAGT 3.561 2.215 1.193 0.4430.288 — 2.916 3.619 30 9 93491981 — 7057 ACCAACACGGYACACTCACAC — — — — —— — — 30 9 93498640 10992831 7058 CTTCCGATTGRACTGTCCCTT 2.673 2.6101.249 1.060 — — 2.490 2.723 30 9 93527276 7024542 7059TACCTTCCAGYGCCATCAGGA 0.827 2.222 1.902 — — — 0.469 0.854 30 9 935486304744272 8468 TGTCATCTGTRTTCTATGATC 0.060 1.674 — — — — 0.683 0.073 30 993563775 2398871 7061 CTACACGAGAYAACTGACATA 1.860 — — — — — 1.439 1.85331 9 110502598 4132110 8469 AAACACATATYCCCCAACTTT — — — — — — — — 31 9110521910 3001140 8470 CTAGTAACTAYTTCAGAGAAA 0.000 — — — — — 0.161 0.00231 9 110542563 4524871 8471 TCAATGAAATSTGGTGCTGGA 0.228 0.246 — — — —0.115 0.199 31 9 110565516 4336672 8472 TCCCCAAAACMCAGGCAAAAA 1.0641.026 0.657 — — — 0.687 1.098 31 9 110586553 4144418 7103ACAGCTAACTYAGAAAGACAG 0.950 1.793 0.854 0.790 — — 1.457 0.988 31 9110612005 10980564 7106 TACGAATTTCRCAGGTTTTCA 0.970 1.939 1.094 0.969 —— 1.181 0.966 31 9 110631784 2254097 8473 CATGAGGTTGRGTAAGACACC 1.4630.682 0.982 — — — 3.044 1.520 31 9 110648651 1409686 7108TGCTCTGGTTMGTATTGGTAG 1.991 0.943 — — — — 1.726 2.021 31 9 1106759824481678 8474 TATAATTTCTYAAGCAGATAC 0.080 — — — — — 0.101 0.063 31 9110688444 512483 8475 CCTCCTCTCASAGAAAAAGAG — — — — — — — — 32 1020313589 1111367 7179 GGACCTCAGAYAGTATAAGTT — — — — — — — — 32 1020324955 7079519 8476 AAGAGGATGGRGTGTCTTATA 0.515 — — — — — 0.441 0.56932 10 20334358 7913119 8477 CACTAGTTGCRGAAACAAAAA 0.838 0.263 — — — —0.470 0.831 32 10 20347228 2884508 8478 TACCTGTTTTSCCCATACTAT 0.1190.335 0.061 — — — 0.122 0.132 32 10 20359198 7907793 8479TATACAAATGRCCATGAATTC 0.945 0.112 0.411 0.269 — — 1.367 0.966 32 1020370658 7070397 8480 AACAGAAATAYTTTTTCTCCC 0.512 0.488 0.109 1.0530.289 — 1.095 0.531 32 10 20382322 7923873 8481 AATTGGTATGRGTTCCTACCA0.022 0.222 0.710 0.283 — — 0.202 0.025 32 10 20395173 6482080 8482CCCTTTGAAGWTCTATTTGTC 0.476 0.181 0.212 — — — 0.312 0.505 32 10 2040643112250505 7189 ATGGGGTTACRATGGGGTTAG — — — — — — — — 32 10 2041822510827961 8483 GTATGAGTCAYGAATGTATTT 0.231 0.303 — — — — 0.046 0.192 3210 20430199 7090580 8484 AGCTCAGCCAKTATCAGAAAG 0.282 — — — — — 0.6430.260 33 10 100843202 2902259 8485 ACTGTTTTGCRCTCCCATGAC 0.568 — — — — —0.352 0.596 33 10 100856514 6584235 8486 TTATAGCTAAKAAAGTTGGGG — — — — —— — — 33 10 100873537 1954295 8487 AGCAGTCCTARTCACAGGCAA 0.884 0.384 — —— — 0.943 0.887 33 10 100889285 10786510 8488 AATTACACTAYAAATCCCATC0.056 1.031 0.527 — — — 0.304 0.061 33 10 100898963 1010357 8489ACTTTTTTAASAACAGAAGCT 0.675 0.544 0.531 0.430 — — 0.367 0.690 33 10100912517 10883285 8490 TCCACTTTGGYTTCAAAATGT — — — — — — — — 33 10100932439 2796759 8491 GATTAACCATYCTATCTTGGA 0.424 0.348 0.633 0.4380.593 — 0.200 0.416 33 10 100947920 4456193 8492 GCAAAGGCCCRGTTTCAAATC0.352 0.156 0.281 0.712 — — 0.175 0.349 33 10 100965767 10786522 8493TCTGATAATGYTGCTGATATT 0.341 0.233 0.397 — — — 0.198 0.374 33 10100978209 2902263 8494 AAAAACATGCWTACAAATAAA 0.389 0.631 — — — — 0.2240.400 33 10 100989074 17094130 7334 GTAAGAAAACRTGAAGATTAA 0.483 — — — —— 0.309 0.481 34 11 11861860 4597067 8495 TCATCCACCARCTAGAGTATA 4.597 —— — — — 3.888 4.627 34 11 11875274 4360694 8496 GGTAACTGGTYCTGTATAGTC —— — — — — — — 34 11 11887874 4910429 7378 TAATTTCATAYAGTGCCTTTG 4.5994.472 — — — — 3.952 4.644 34 11 11896387 12225231 8497AAGTGGCCTCYGTTCTTCAAA — — — — — — — — 34 11 11911794 6485303 7379TGAGACATAAYTGGTGTCAAT 0.101 4.496 3.775 — — — 0.155 0.116 34 11 1193055516910238 7380 ATAGGAGCCARTAGAGAAACA — — — — — — — — 34 11 119362501979687 7381 ACTTGCTATCRCACAAAAGAG 4.444 4.422 2.992 2.498 — — 3.7714.470 34 11 11944883 7480000 7382 AGCATCAACASGGGAAAAAAA 0.961 3.3612.452 2.456 1.793 — 0.871 0.954 34 11 11959842 7479744 8498AAAGGCCTGTYTCATATCCCT 0.405 0.810 3.168 1.918 — — 0.410 0.395 34 1111973956 11601753 8499 CATCAGCCGAYGTGCTCAGGT 0.113 0.178 0.172 — — —0.160 0.117 34 11 11990200 4757949 8500 CTTTTTTCTTRTATCTGTTCT 0.0000.004 — — — — 0.017 0.016 34 11 11998072 4500466 7389TTCCTCTATCRTTGTCATGCT 0.141 — — — — — 0.143 0.160 35 11 1961443316937060 7436 TACAAAGCGGMTCTTGGTCTG — — — — — — — — 35 11 196302481559667 7437 CCAATTAAATRAAGCCTATTA 1.521 — — — — — 1.113 1.570 35 1119638525 16937087 7438 CTGGACATTAKGGTTCATACG — — — — — — — — 35 1119647977 10833153 7439 TCCTTCCTCCYTTGTCCTGGG 0.627 0.954 — — — — 1.0090.628 35 11 19653940 2216997 7440 TACAGCAATTKGTAATTCACG 1.664 0.9361.279 — — — 1.042 1.549 35 11 19659944 7113050 7441AGACCTGTGGRCACATCTGAA 1.201 1.729 1.375 0.961 — — 0.789 1.243 35 1119663584 752459 7442 AGGTATGGCCRGGCTGAGGAG 3.303 1.588 1.083 — — — 2.6313.323 35 11 19679410 1364790 8501 GCAAGGAAAGYGCTCAAATGG 2.817 1.779 — —— — 2.233 2.878 35 11 19687211 890136 7443 CTTATAGGCCRTTTTTATAAT 2.678 —— — — — 2.452 2.617 35 11 19696994 — 7444 CACAAATATAYACACATGGAA — — — —— — — — 35 11 19707031 1559665 7445 CAAGGAACGTRTGCAGAGAAA — — — — — — —— 35 11 19721589 1346028 8502 TCCTGGTGCTYTCTCCCTCCT — — — — — — — — 3511 19731509 1834323 8503 CATACATTTTYTTATATTTTG — — — — — — — — 36 1195500304 7951852 7557 TTCCATCAGCRCCAAATAATT 0.197 — — — — — 0.346 0.22336 11 95507337 7951538 8504 ATATTTGAAGSCAGTCAAACT 0.793 0.219 — — — —0.472 0.798 36 11 95516314 544552 8505 AGGGATTTCCRATTTCCATAA 0.122 0.6150.629 — — — 0.974 0.130 36 11 95524215 515200 8506 TCTTGACAGCWAGTAATCCGA0.653 1.180 0.666 0.539 — — 0.949 0.721 36 11 95532144 1262181 8507TTTCCAGGCGRATTTGTTGTC 0.631 1.248 0.751 0.455 0.230 — 0.364 0.663 36 1195541663 2155001 7561 GTGCTGGCTGSTGTGGGGATT 0.188 0.415 1.077 0.3330.203 0.056 0.434 0.198 36 11 95550186 7111187 8508GGCATGTCACMGGGAAAGAAG 0.686 0.075 0.098 0.419 0.151 — 0.405 0.727 36 1195558601 495494 8509 CCTCTTTTGGKCCCTCCAGTC 0.357 0.080 0.005 0.006 — —0.147 0.390 36 11 95567591 573424 8510 GGAAGAAAAGRCACCACCGAA 0.354 0.1960.021 — — — 0.572 0.342 36 11 95576367 505879 8511 GGAGCAAGCCWTTACAAAAAA0.509 0.055 — — — — 0.623 0.510 36 11 95585236 575232 8512GGATCCAGCARAGAAGGAGCA 0.588 — — — — — 0.302 0.602 37 12 56202309 11485578513 AGATGGTCTCYCCTGGCCTAA 0.561 — — — — — 0.312 0.603 37 12 562263381669297 8514 TGACAGCGGARACAATTGGGA 0.576 1.709 — — — — 0.312 0.603 37 1256254982 1678542 7613 TGAAATGCCGSTGATAGAGAG 1.975 1.178 0.584 — — —1.576 2.014 37 12 56271471 11537654 8515 TCCGGGCGGCYGACCCGCTGG 0.8440.725 0.089 0.408 — — 0.519 0.889 37 12 56299442 2277324 7617TCCAAGCCGCRGCTCTCGCCA 0.894 0.150 0.564 0.310 0.238 — 0.523 0.922 37 1256307200 10083154 7618 ATATACTTGTRCTTTGAAAAT 0.993 0.299 0.167 0.5550.381 0.414 0.595 0.994 37 12 56335586 1678520 8516CCTTCCTCCCSCTATTTTTAA 1.191 0.551 0.423 0.164 0.788 — 0.784 1.237 37 1256351715 2640629 7619 GTGTAATAAAYAAAAGGCAAC 1.216 0.716 0.344 0.182 — —0.819 1.276 37 12 56376989 7309600 7620 GCATCCCTGCRAACAGCTTCT 0.1930.546 0.381 — — — 0.133 0.211 37 12 56393103 701006 7621AGGGCAATATRTAGGTTTGTT 1.414 0.646 — — — — 1.027 1.453 37 12 564309322069502 8517 CACTCTCCTAYTCCCAACCAG 0.940 — — — — — 0.683 0.987 39 1449806475 2153553 7751 TGAGCAATTAYCGTGTTACTC 1.031 — — — — — 0.629 1.02039 14 49822992 11849603 8518 GGAAAAAAAASAAGTGACCCC 1.140 0.898 — — — —0.747 1.167 39 14 49839467 2297995 7754 CGCCACCAACRATGACTATAT 1.1690.881 0.261 — — — 0.777 1.206 39 14 49857963 2275592 7756CAAATGATGCYGTTTGGAAAA 1.059 0.478 0.323 0.199 — — 0.683 1.088 39 1449868493 4901016 7757 CATGCAGGCTRGAAACCCTGG — — — — — — — — 39 1449880775 1465160 7758 CTGCAACTGAYATACTGCAGC 0.817 0.222 0.303 0.4620.263 — 0.446 0.837 39 14 49894783 11570816 8519 CCGGATTGCTYCCAGTGAAAT0.215 0.089 0.350 0.306 0.397 — 1.043 0.235 39 14 49904329 9635166 7761GACTCAGATTKACTTTCTCTT 0.709 0.982 0.135 0.463 — — 0.575 0.717 39 1449917611 6572667 8520 GACCCAGGACRAGAGGGAACT 0.650 0.458 0.498 — — —0.773 0.604 39 14 49927723 1538904 8521 ACCTGAGTTAYGGAAAGGAGT 0.2400.407 — — — — 0.098 0.236 39 14 49937222 10149335 8522TGTGTAGGACRTTGAGAGCTG 0.112 — — — — — 0.372 0.110 40 14 80648058 28880497822 GAAGTGGTCARTTAAACTACC 0.410 — — — — — 1.212 0.438 40 14 806615077158881 7823 GTAAAGATGGYCCCTGTGGAA 0.490 1.018 — — — — 0.712 0.508 40 1480668665 17111530 7824 GTTACTTTCAYTAGACACTTA 0.955 0.436 2.438 — — —0.729 0.949 40 14 80677583 2300541 7825 TGCCTGTATGKTAGTGTCCAT 0.0180.953 0.653 2.815 — — 0.229 0.032 40 14 80690636 28441485 7826CTGTTAGGGGKAAGTTTAATG 1.473 0.538 1.185 1.265 2.952 — 0.982 1.484 40 1480699947 12372876 7827 AATCAAAACAYGGCAAAGGGA 1.456 1.777 0.818 0.8321.696 2.860 0.981 1.485 40 14 80709737 1957546 7828AGTCACTTCAYCAAGACTGTA 2.667 1.873 1.327 1.028 1.593 1.970 2.046 2.718 4014 80713553 4899786 7829 TGGCCACCAAKCAAGAGTTTG 2.847 2.260 1.879 1.9241.539 1.998 2.180 2.865 40 14 80727089 7149672 7830ATGTTACTTCRAGAAGCACTG 2.147 1.951 2.136 2.643 2.096 — 1.520 2.161 40 1480751275 6574632 8523 GGAATATCAAWCAATCAAATT 0.830 1.916 1.956 1.617 — —0.634 0.868 40 14 80774435 8021385 8524 AAACTTCAAARGATTTAAATT 1.2361.617 1.196 — — — 0.790 1.242 40 14 80797377 7149917 8525GCTTCAGTCAYGGTGTGGGGC — — — — — — — — 40 14 80819977 7148483 8526AAGTCTGTCARCACCATCTTT 1.699 1.546 — — — — 1.565 1.713 40 14 808337081885604 7840 GCATGCTATAYGATTTAAATT 3.552 — — — — — 3.054 3.432 42 1673722875 7206259 7908 AGAAGCATCAYGTTGCTAACA 0.032 — — — — — 0.011 0.03242 16 73735820 7198880 8527 CATATCACCASAATTTATTTT — — — — — — — — 42 1673761944 12935567 7909 ATATATCCAGYTGTAAATAGC 0.633 0.484 — — — — 0.2770.594 42 16 73762714 17673793 7910 CAGAGACTTTRGAGGAAAAAT 0.270 1.3560.374 — — — 0.183 0.245 42 16 73787731 1559362 7911TGGTTTTGCCYGTGTGACCAA 0.370 0.174 0.881 0.616 — — 0.695 0.406 42 1673789787 10514392 7912 TCTTGCGACTRCTCCAGACGT 0.108 0.744 0.980 1.7050.943 — 0.089 0.141 42 16 73812718 8051363 7913 TTCTGCTTCCRATGTGCCATT0.759 0.490 1.465 1.170 — — 0.409 0.743 42 16 73823220 8062565 7914TCTGAAAACAMGGCGATCCCA — — — — — — — — 42 16 73837879 28439846 7915GGTCAGAGACRGCCTCCCCAA 2.033 1.989 0.829 — — — 1.306 1.941 42 16 738517124261573 7916 TTTGATTCGAYTGATGGCTAC 0.029 0.835 — — — — 0.882 0.045 42 1673878532 4243112 7918 CTGAGCAACCYTTCAAAGGTG 0.613 — — — — — 0.331 0.63842 16 73890482 11149814 7920 CACCAAGAAAYCTGTGTAATG — — — — — — — — 43 1676574323 402904 7956 GCCATTTGGGRTATAGGAAAC 1.514 — — — — — 1.289 1.54243 16 76594303 8058458 7959 CAATCCTATCKGTGAATTTCT 1.035 0.815 — — — —0.689 1.037 43 16 76612446 11643023 7962 CCACTGCCCTYCATTTGCACC 0.4100.690 1.089 — — — 0.248 0.398 43 16 76634316 4888726 7963ATTGGGGCCAYCCTGTGCATT 0.646 0.695 1.265 0.525 — — 1.037 0.635 43 1676660674 6564507 7965 CTTGTCATTCRATCTTGTCTA 1.256 0.875 0.894 0.646 — —0.904 1.278 43 16 76673175 16947096 7966 TCAGGAACGAYGATTTATAAC 0.5230.251 0.124 — — — 0.347 0.537 43 16 76680185 — 7967GGGCCTATGCRTAGAGTCAGT — — — — — — — — 43 16 76685815 1073111 7968CTCCTTGCTARTAATCAATGT 0.386 0.038 — — — — 0.368 0.414 43 16 766986078045088 7969 CCCTTCGAATRCCTCTTGTAG — — — — — — — — 43 16 767179638057015 8528 CATGTGCTATMATTTAATTTT 0.111 — — — — — 0.146 0.142 44 1822743410 162632 8147 TTAATTCTGAYACAGGTCGTC 0.887 — — — — — 0.684 0.90244 18 22768586 163057 8529 AACCCGAAGAKCCTGGAGAGA 2.039 0.584 — — — —1.828 2.089 44 18 22793630 12326470 8530 AAATGGTTGCRCTGAACTTAA 0.2550.835 0.233 — — — 0.087 0.274 44 18 22821237 1175739 8531CTCTTTGCACRTGTCTGCTTC 0.000 0.130 0.655 1.250 — — 0.462 0.013 44 1822845245 1185795 8532 TCTTGGTGAAKGCAGGAATTA 0.039 0.075 2.500 1.4431.174 — 0.336 0.042 44 18 22868217 1941114 8533 TCTCTGTTTTYGTATCCTTCC0.092 3.554 1.977 1.771 1.436 1.068 0.367 0.107 44 18 22898135 65084678167 AAACCCTGGGMAAGTGATAAT 4.804 3.408 2.199 1.858 1.305 — 4.259 4.80744 18 22917032 1385753 8534 TGTGAAGAGAYAGCCGTAAGG 0.332 3.064 2.7271.660 — — 0.343 0.387 44 18 22940785 4800789 8535 TAATCTACCTRCTTACTACCC0.087 0.238 2.271 — — — 0.185 0.103 44 18 22964271 2114471 8536TGACCTAGTARTTATTTTAAT 0.187 0.095 — — — — 0.195 0.222 44 18 229817454800791 8537 TCCTCTGTAARCCCCTACAGT 0.430 — — — — — 0.216 0.470 45 1861556288 7226378 8227 AAGCCCTTAARGAAATATAGA 0.454 — — — — — 2.031 0.47845 18 61577193 12963820 8538 AATGAGTTGCWCAATTATCAA 0.770 0.814 — — — —2.550 0.772 45 18 61595696 1484725 8231 CTCCAAACGCRAAGCAAATTT 1.1061.803 0.806 — — — 2.741 1.114 45 18 61621115 4334398 8235TCGGTGAACAYGAAGCTCTAA 0.867 1.883 1.139 0.598 — — 0.548 0.875 45 1861627292 11659738 8236 TTGAAGTCCCRTGCATGTATT 0.895 1.364 0.641 0.8580.972 — 0.538 0.911 45 18 61634277 4144764 8237 CCTATGGAGAYAAAGCCATAC —— — — — — — — 45 18 61635487 6566179 8238 TAAGAGGTATYGTCATAACAA — — — —— — — — 45 18 61655933 9963814 8539 AAACATAAGGYCTTCTTGGTC 1.401 1.2711.106 0.898 — — 0.889 1.418 45 18 61672858 1484711 8239AGTTCAAGGCYTTTTAGCTTT 2.119 1.231 0.841 — — — 1.603 2.175 45 18 616869972587410 8241 GGAAACTTTTYTTCTATATTA 2.107 1.930 — — — — 1.603 2.175 45 1861704351 1843184 8540 TTATAGCTTCRTGATTAAAAT 0.684 — — — — — 0.443 0.65945 18 61717080 7407658 8541 ACAAGGAAAAKATCTTTAAAC — — — — — — — — 47 X80276252 1538351 8542 GTGACAGAGASAGAACATGAA 0.129 — — — — — — — 47 X80303261 12848561 8543 CTGTCACTCARTGAAGCTCAT 0.087 1.088 — — — — — — 47X 80325649 5959088 8336 CTGTGTGCATRCTGCCTGTGT 0.302 0.624 0.986 — — — —— 47 X 80348471 4826212 8338 CTGTTTGATTRGATGACTCAT 1.139 0.582 0.4250.578 — — — — 47 X 80387275 5959099 8340 TTTTAATCTARCTCTTTATTC 1.1150.602 0.115 0.707 0.977 — — — 47 X 80411297 5912496 8342AAGGATGGATRTAACGGAGAG 0.879 0.455 0.602 0.582 0.674 — — — 47 X 804353146616745 8345 TTGAAGGACAYAGATGGATAC 0.989 0.670 0.644 0.636 — — — — 47 X80466657 — 8348 GCTGTTATTTWTTTCCCTAGC — — — — — — — — 47 X 804923532602598 8350 GTAGTGTGCTRGATAACAGAG 1.787 1.441 0.709 — — — — — 47 X80525324 1597965 8544 CAAGAGTGATRTTAGCTATGT 1.787 1.437 — — — — — — 47 X80552137 5913497 8545 CACACTTATTRTCAGTCTCAC 1.713 — — — — — — —

TABLE 4 List of longevity candidate genes from the regions identifiedfrom the genome wide and fine mapping association analysis. The firstcolumn corresponds to the region identifier provided in Table 1. Thesecond and third columns correspond to the chromosome and cytogeneticband, respectively. The fourth and fifth columns corresponds to thechromosomal start coordinates of the NCBI genome assembly derived frombuild 35 (B35) and the end coordinates (the start and end positionrelate to the + orientation of the NCBI assembly and don't necessarilycorrespond to the orientation of the gene). The sixth and seventhcolumns correspond to the official gene symbol and gene name,respectively, and were obtained from the NCBI Entre2 Gene database. Theeighth column corresponds to the NCBI Entrez Gene Identifier (Gene ID).The ninth and tenth columns correspond to the Sequence IDs fromnucleotide (cDNA) and protein entries in the Sequence Listing. Start EndPosition position Entrez Region ID Chromosome Cytogenetic Band B35 B35Gene Symbol Gene Name Gene ID Nucleotide Seq ID Protein Seq ID 1 11q25.2 172931977 172932623 LOC391140 similar to ribosomal protein L13391140 — — 1 1 1q23-q25 173163964 173543629 PAPPA2 pappalysin 2 60676 1,3 2, 4 1 1 1q25.2 173561861 173865681 ASTN astrotactin 460 5, 7 6, 8 2 11q42-q43 225713614 225747253 RAB4A RAB4A, member RAS oncogene 5867  9 10 family 2 1 1q42.11-q42.3 225747016 225747983 SPHAR S-phase response(cyclin- 10638  11  12 related) 2 1 1q42.13 225766950 225784999 C1orf96chromosome 1 open rending 126731  13  14 frame 96 2 1 1q42.13-q42.2225873730 225876578 ACTA1 actin alpha 1, skeletal muscle 58  15  16 2 11q42.13 225883779 225950823 NUP133 nucleoporin 133 kDa 55746  17  18 2 11q42 225959065 226001177 ABCB10 ATP-binding cassette, sub-family 23456 19  20 B (MDR/TAP), member 10 3 1 1q42.2 227084993 227136463 COG2component of oligomeric golgi 22796  21  22 complex 2 3 1 1q42-q43227145020 227156602 AGT angiotensinogen (serpin 183  23  24 peptidaseinhibitor, clade A, member 8) 3 1 1q42.11-q42.3 227189865 227244243CAPN9 calpain 9 10753 25, 27 26, 28 3 1 1q42.13-q43 227279601 227311012C1orf198 chromosome 1 open reading 84886  29  30 frame 198 3 1 1q42.2227348725 227421319 TTC13 tetratricopeptide repeat domain 79573  31  3213 3 1 1q42.2 227421558 227443214 ARV1 ARV1 homolog (yeast) 64801  33 34 3 1 1q42.2 227461670 227482720 FAM89A family with sequencesimilarity 375061  35  36 89, member A 3 1 1q42.2 227483511 227605268LOC441924 similar to peptidylprolyl 441924 — — isomerase A isoform 1 4 11q41-q44 236396793 236398565 CHRM3 cholinergic receptor, muscarinic 31131  37  38 4 1 1q43 236460221 236463588 LOC128136 similar toHydroxymethylglutaryl- 128136 — — CoA synthase, cytoplasmic (HMG-CoAsynthase) (3-hydroxy- 3-methylglutaryl coenzyme A synthase) 4 1 1q43236502010 236964518 FMN2 formin 2 56776  39  40 4 1 1q43 236631871236634716 LOC266783 proteasome 26S non-ATPase 266783 — — subunit 2pseudogene 5 2 2p24.1 20806446 20922853 FLJ21820 hypothetical proteinFLJ21820 60526  41  42 5 2 2p24-p23 21135953 21178597 APOBapolipoprotein B (including Ag(x) 338  43  44 antigen) 6 2 2p16.350017421 50018157 LOC130728 similar to 60S ribosomal protein 130728 — —L7 6 2 2p16.3 50059139 51167254 NRXN1 neurexin 1 9378 45, 47 46, 48 7 22p16.3 50059139 51167254 NRXN1 neurexin 1 9378 45, 47 46, 48 8 2 2q14121694764 121759005 TFCP2L1 transcription factor CP2-like 1 29842  49 50 8 2 2q14.2-q14.3 121811585 122123282 CLASP1 cytoplasmic linkerassociated 23332  51  52 protein 1 8 2 2q14.3 122182315 122186434LOC440902 similar to Nucleophosmin (NPM) 440902  53  54 (Nucleolarphosphoprotein B23) (Numatrin) (Nucleolar protein NO38) 8 2 2q14.3122200784 122210698 MKI67IP MKI67 (FHA domain) interacting 84365  55  56nucleolar phosphoprotein 8 2 2q21.1 122229351 122241659 TSN translin7247  57  58 9 2 2q14 127521837 127581094 BIN1 bridging integrator 1 27459, 61, 63, 65, 67, 69, 71, 73, 60, 62, 64, 66, 68, 70, 75, 77 72, 74,76, 78 9 2 2q14.3 127657638 127700162 FLJ16008 FLJ16008 protein 339761 79  80 9 2 2q21 127731096 127767982 ERCC3 excision repair cross- 2071 81  82 complementing rodent repair deficiency, complementation group 3(xeroderma pigmentosum group B complementing) 9 2 2q14.3 127780008127817035 MAP3K2 mitogen-activated protein kinase 10746  83  84 kinasekinase 2 9 2 2q13-q14 127892247 127903048 PROC protein C (inactivator of5624  85  86 coagulation factors Va and VIIIa) 9 2 2q14.3 127954620128000274 IWS1 hypothetical protein LOC55677 55677  87  88 10 2 2q24.2161990447 162093433 PSMD14 proteasome (prosome, 10213  89  90 macropain)26S subunit, non- ATPase, 14 10 2 2q24 162098127 162107080 TBR1 T-box,brain, 1 10716  91  92 10 2 2q24.2 162182642 162189817 AHCTF1P AT hookcontaining transcription 285118 — — factor 1 pseudogene 10 2 2q24.2162262089 162263367 LOC391458 similar to keratin complex 1, 391458 — —acidic, gene 18 10 2 2q23-q24 162306453 162665750 SLC4A10 solute carrierfamily 4, sodium 57282  93  94 bicarbonate transporter-like, member 1010 2 2q24.3 162674264 162756559 DPP4 dipeptidyl-peptidase 4 (CD26, 1803 95  96 adenosine deaminase complexing protein 2) 10 2 2q24.2 162759374162759688 DDPP deafness dystonia pseudogene 399520 — — 10 2 2q36-q37162824895 162834264 GCG glucagon 2641 97 98 10 2 2q23 162852709162925552 FAP fibroblast activation protein, 2191  99 100 alpha 10 22p24.3-q24.3 162949097 163000546 IFIH1 interferon induced with helicase64135 101 102 C domain 1 11 2 2q33 198773985 198838554 PLCL1phospholipase C-like 1 5334 103 104 12 3 3p25.3 6789724 6790033 MRPS36P1mitochondrial ribosomal protein 347705 — — S36 pseudogene 1 12 33p26.1-p25.1 6877927 7758217 GRM7 glutamate receptor, metabotropic 72917 105, 107, 109 106, 108, 110 13 3 3p22.3 32701702 32790358 CNOT10CCR4-NOT transcription 25904 111 112 complex, subunit 10 13 3 3p22.332799068 32806379 LOC389101 similar to 60S ribosomal protein 389101 — —L23a 13 3 3p22.3 32832845 32833862 UNQ6490 similar to YPLR6490 389102113 — 13 3 3p22.3 32834892 32908307 LIN41 abnormal cell LINeage LIN-41131405 114 115 13 3 3p24 32968056 32971404 CCR4 chemokine (C-C motif)receptor 4 1233 116 117 13 3 3p21.33 33013236 33113635 GLB1galactosidase, beta 1 2720 118 119 13 3 3p22.3 33130571 33160004 CRTAPcartilage associated protein 10491 120 121 13 3 3p22.3 33166541 33246551SUSD5 sushi domain containing 5 26032 122 123 13 3 3p22.3 3329393833403129 FBXL2 F-box and leucine-rich repeat 25827 124 125 protein 2 143 3p22.1 39826307 40276816 MYRIP myosin VIIA and Rab interacting 25924126 127 protein 14 3 3p22.1 40326194 40328902 EIF1B eukaryotictranslation initiation 10289 128 129 factor 1B 14 3 3p21.3 4040369440445114 ENTPD3 ectonucleoside triphosphate 956 130 131diphosphohydrolase 3 14 3 3p22-p21.2 40473819 40478863 RPL14 ribosomalprotein L14 9045 132, 134 133, 135 14 3 3p22.1 40493641 40504881 ZNF619zinc finger protein 619 285267 136 137 14 3 3p22.1 40522487 40534204ZNF620 zinc finger protein 620 253639 138 139 14 3 3p22.1 4054137340550438 ZNF621 zinc finger protein 621 285268 140 141 14 3 3p22.140612702 40632425 LOC442079 similar to Elongation factor 1- 442079 — —gamma (EF-1-gamma) (eEF-1B gamma) 15 4 4q21.1 77007008 77092543 VDPvesicle docking protein p115 8615 142 143 15 4 4q21.1 77138207 77180860PPEF2 protein phosphatase, EF-hand 5470 144, 146, 148 145, 147, 149calcium binding domain 2 15 4 4q21.1 77195804 77219333 ASAHLN-acylsphingosine 27163 150 151 amidohydrolase (acid ceramidase)-like 154 4q21.1 77228248 77260314 SDAD1 SDA1 domain containing 1 55153 152 15315 4 4q21 77279802 77285820 CXCL9 chemokine (C—X—C motif) ligand 9 4283154 155 15 4 4q21 77299452 77301829 CXCL10 chemokine (C—X—C motif)ligand 3627 156 157 10 15 4 4q21.2 77312021 77314412 CXCL11 chemokine(C—X—C motif) ligand 6373 158 159 11 15 44p15.1-p14|4p15.1-p14|4p15.1-p14 77353042 77391120 ART3ADP-ribosyltransferase 3 419 160 161 15 4 4q21.1 77392997 77426834 NUP54nucleoporin 54 kDa 53371 162 163 15 4 4q21.1 77437073 77492214 SCARB2scavenger receptor class B, 950 164 165 member 2 16 4 4q24 100853159100901804 MTTP microsomal triglyceride transfer 4547 166 167 protein 164 4q23 100914872 100933921 LOC285556 hypothetical protein LOC285556285556 168 169 16 4 4q25-q27 101095168 101148489 DAPP1 dual adaptor ofphosphotyrosine 27071 170 171 and 3-phosphoinositides 16 4 4q23101159543 101172881 MAP2K1IP1 mitogen-activated protein kinase 8649 172173 kinase 1 interacting protein 1 16 4 4q23 101177747 101205426 DNAJB14DnaJ (Hsp40) homolog, 79982 174, 176 175, 177 subfamily B, member 14 164 4q24 101226421 101228611 H2AFZ H2A histone family, member Z 3015 178179 17 4 4q28.1 126595172 126600361 LOC339951 similar toCadherin-related tumor 339951 180 181 suppressor precursor (Fat protein)17 4 4q28.1 126672696 126770528 FAT4 FAT tumor suppressor homolog 79633182 183 4 (Drosophila) 18 4 4q31.21 143307499 143710137 INPP4B inositolpolyphosphate-4- 8821 184 185 phosphatase, type II, 105 kDa 19 6 6p22.127208074 27208554 HIST1H2BJ histone 1, H2bj 8970 186 187 19 6 6p22.127208800 27211050 HIST1H2AG histone 1, H2ag 8969 188 189 19 6 6p21.3327214052 27222598 HIST1H2BK histone 1, H2bk 85236 190 191 19 6 6p21.3327215067 27215436 HIST1H4I histone 1, H4i 8294 192 193 19 6 6p21.3327222887 27223373 HIST1H2AH histone 1, H2ah 85235 194 195 19 6 6p22.127287002 27287670 LOC442171 similar to ribosomal protein L10 442171 — —19 6 6p21 27323487 27332229 PRSS16 protease, serine, 16 (thymus) 10279196 197 19 6 6p22.1 27343934 27344990 LOC442172 similar to cell divisioncycle 442172 — — associated 7 19 6 6p22.1 27361661 27388776 LOC441135similar to Nuclear envelope pore 441135 198 199 membrane protein POM 121(Pore membrane protein of 121 kDa) (P145) 19 6 6p22.1 27400557 27401721FKSG83 FKSG83 83954 200 201 19 6 6p21.3 27433582 27447283 ZNF204 zincfinger protein 204 7754 202 — 20 6 6q25.1 152220800 152516520 ESR1estrogen receptor 1 2099 203 204 20 6 6q25 152534937 153050648 SYNE1spectrin repeat containing, 23345 205, 207, 209, 211 206, 208, 210, 212nuclear envelope 1 20 6 6q25 152959863 152960263 NANOGP11 NANOG homeoboxpseudogene 414135 — — 11 20 6 6q25.2 153111152 153136877 MYCT1 myctarget 1 80177 213 214 20 6 6q25 153164047 153173014 VIP vasoactiveintestinal peptide 7432 215, 217 216, 218 21 7 7p15.1 30370952 30385944LOC401320 hypothetical LOC401320 401320 219 220 21 7 7p15 3040742130446884 GARS glycyl-tRNA synthetase 2617 221 222 21 7 7p15.1 3046544030495136 CRHR2 corticotropin releasing hormone 1395 223 224 receptor 221 7 7p15.3-p15.2 30564991 30570462 INMT indolethylamino N- 11185 225226 methyltransferase 21 7 7p15.1 30584280 30705242 FLJ22374hypothetical protein FLJ22374 84182 227 228 21 7 7p14 30724708 30738371AQP1 aquaporin 1 (Colton blood group) 358 229 230 21 7 7p14 3077687630792383 GHRHR growth hormone releasing 2692 231, 233 232, 234 hormonereceptor 21 7 7p14 30865382 30919551 ADCYAP1R1 adenylate cyclaseactivating 117 235 236 polypeptide 1 (pituitary) receptor type I 22 77p14.3 32681024 32704612 KBTBD2 Kelch repeat and BTB (POZ) 25948 237 238domain containing 2 22 7 7p14.3 32730196 32755873 LOC441212 PNAS-13441212 239 240 22 7 7p11.1 32770292 32819782 FKBP9 FK506 binding protein9, 63 kDa 11328 241 242 22 7 7p14.3 32826991 32853758 NT5C35-nucleotidase, cytosolic III 51251 243, 245, 247 244, 246, 248 22 77p14.3 32907652 32922242 RP9 retinitis pigmentosa 9 (autosomal 6100 249250 dominant) 22 7 7p14 32942414 33418920 PTHB1 parathyroidhormone-responsive 27241 251, 253, 255, 257 252, 254, 256, 258 B1 23 77q31.3 126886185 127326610 SND1 staphylococcal nuclease domain 27044 259260 containing 1 23 7 7q31.3 127261077 127264953 LRRC4 leucine richrepeat containing 4 64101 261 262 23 7 7q31.3 127485995 127491632 LEPleptin (obesity homolog, mouse) 3952 263 264 23 7 7q32.1 127544388127577913 RBM28 RNA binding motif protein 28 55131 265 266 23 7 7q32.1127584861 127595686 LOC401399 hypothetical gene supported by 401399 267,269 268, 270 BC063892 23 7 7q31.3-q32 127626283 127644257 IMPDH1 IMP(inosine monophosphate) 3614 271, 273 272, 274 dehydrogenase 1 23 77q32.1 127689896 127692421 HIG2 hypoxia-inducible protein 2 29923 275276 23 7 7q32.1 127710769 127736536 METTL2B methyltransferase like 2B55798 277 278 24 7 7q35-q36 145251477 147555734 CNTNAP2 contactinassociated protein-like 2 26047 279 280 24 7 7q36.1 147569287 147580921LOC392145 similar to Mtr3 (mRNA transport 392145 281 282 regulator3)-homolog 24 7 7q36.1 147725305 147750600 C7orf33 chromosome 7 openreading 202865 283 284 frame 33 25 7 7q31-q35 149458253 149472887 LRRC61leucine rich repeat containing 61 65999 285 286 25 7 7q36.1 149465049149467458 C7orf29 chromosome 7 open reading 113763 287 288 frame 29 25 77q36.1 149473067 149476354 RARRES2 retinoic acid receptor responder 5919289 290 (tazarotene induced) 2 25 7 7q36.1 149505509 149508776 REPIN1replication initiator 1 29803 291, 293 292, 294 25 7 7q36.1 149514098149533366 MGC33584 hypothetical protein MGC33584 285971 295 296 25 77q36.1 149585772 149614129 GIMAP8 GTPase, IMAP family member 8 155038297 298 25 7 7q36.1 149649611 149655809 GIMAP7 GTPase, IMAP familymember 7 168537 209 300 25 7 7q36 149676368 149676891 ATQL3antiquitin-like 3 543 — — 25 7 7q36.1 149702106 149708689 GIMAP4 GTPase,IMAP family member 4 55303 301 302 25 7 7q36.1 149820442 149828373GIMAP2 GTPase, IMAP family member 2 26157 303 304 25 7 7q36.1 149851346149855901 GIMAP1 GTPase, IMAP family member 1 170575 305 306 25 7 7q36.1149872148 149878384 GIMAP5 GTPase, IMAP family member 5 55340 307 308 257 7q36.1 149926038 149936011 LR8 LR8 protein 28959 309 310 25 7 7q36.1149935502 149939856 HCA112 hepatocellular carcinoma- 55365 311 312associated antigen 112 26 7 7q35-q36 150079698 150112662 KCNH2 potassiumvoltage-gated 3757 313, 315, 317 314, 316, 318 channel, subfamily H(eag- related), member 2 26 7 7q36 150125796 150149324 NOS3 nitric oxidesynthase 3 4846 319 320 (endothelial cell) 26 7 7q36 150163185 150180300ABCB8 ATP-binding cassette, sub-family 11194 321 322 B (MDR/TAP), member8 26 7 7q35 150183600 150187489 ACCN3 amiloride-sensitive cation 9311323, 325, 327 324, 326, 328 channel 3 26 7 7q36 150188547 150192644 CDK5cyclin-dependent kinase 5 1020 329 330 26 7 7q35-q36 150194387 150211258SLC4A2 solute carrier family 4, anion 6522 331 332 exchanger, member 2(erythrocyte membrane protein band 3-like 1) 26 7 7q35 150211356150215599 FASTK Fas-activated serine/threonine 10922 333, 335 334, 336kinase 26 7 7q36.1 150215821 150217736 C7orf21 chromosome 7 open reading83590 337 338 frame 21 26 7 7q36.1 150221474 150279171 CENTG3 centaurin,gamma 3 116988 339 340 26 7 7q36.1 150310433 150322126 ASB10 ankyrinrepeat and SOCS box- 136371 341 342 containing 10 26 7 7q36.1 150325611150340230 LOC346545 similar to IQ motif containing with 346545 343 344AAA domain 26 7 7q36 150342571 150361965 ABCF2 ATP-binding cassette,sub-family 10061 345, 347 346, 348 F (GCN20), member 2 26 7 7q36.1150367223 150373553 CSGlcA-T chondroitin sulfate 54480 349 350glucuronyltransferase 26 7 7q35-q36 150373707 150409988 SMARCD3 SWI/SNFrelated, matrix 6604 351, 353, 355 352, 354, 356 associated, actindependent regulator of chromatin, subfamily d, member 3 26 7 7q36150476506 150513183 NYREN18 NEDD8 ultimate buster-1 51667 357 358 26 77q36.1 150515862 150545388 LOC349136 hypothetical protein LOC349136349136 359 360 27 7 7q36.3 156629186 156709609 DNAJB6 DnaJ (Hsp40)homolog, 10049 361, 363 362, 364 subfamily B, member 6 27 7 7q36156831231 157879894 PTPRN2 protein tyrosine phosphatase, 5799 365, 367,369 366, 368, 370 receptor type, N polypeptide 2 27 7 7q36.3 157912773157915710 LOC285872 THAP5 pseudogene 285872 — — 27 7 7q36.3 157936457157996965 LUZP5 leucine zipper protein 5 54892 371 372 27 7 7q36.3158023165 158121795 FAM62B family with sequence similarity 62 57488 373374 (C2 domain containing) member B 28 9 9p21.2 26831252 26882800C9orf82 chromosome 9 open reading 79886 375 376 frame 82 28 9 9p2126894518 26925207 PLAA phospholipase A2-activating 9373 377, 379 378,380 protein 28 9 9p21.2 26946410 27052802 IFT74 intraflagellar transport74 80173 381 382 homolog (Chlamydomonas) 28 9 9p21.2 26983588 26995670LRRC19 leucine rich repeat containing 19 64922 383 384 28 9 9p2127099441 27220165 TEK TEK tyrosine kinase, endothelial 7010 385 386(venous malformations, multiple cutaneous and mucosal) 28 9 9p2127235682 27272791 C9orf14 chromosome 9 open reading 158035 387 388 frame14 28 9 9p21 27274667 27287137 C9orf11 chromosome 9 open reading 54586389 390 frame 11 29 9 9q21.11 70103452 70196890 SMC5L1 SMC5 structuralmaintenance of 23137 391 392 chromosomes 5-like 1 (yeast) 29 9 9q1370229379 70259094 KLF9 Kruppel-like factor 9 687 393 394 29 99q21.11-q21.12 70380423 70713500 TRPM3 transient receptor potentialcation 80036 395, 397, 399, 401, 403, 405, 396, 398, 400, 402, 404,channel, subfamily M, member 3 407, 409, 411 406, 408, 410, 412 30 99q22.31 93288338 93295429 C9orf10OS chromosome 9 open reading 158293 413414 frame 10 opposite strand 30 9 9q22.31 93293728 93407949 C9orf10chromosome 9 open reading 23196 415 416 frame 10 30 9 9q22.31 9341858593521421 PHF2 PHD finger protein 2 5253 417, 419 418, 420 31 99q31.3-q32 110510694 110642833 MUSK muscle, skeletal, receptor 4593 421422 tyrosine kinase 31 9 9q31.3 110715611 110879920 EDG2 endothelialdifferentiation, 1902 423, 425 424, 426 lysophosphatidic acid G-protein-coupled receptor, 2 32 10 10p12.33-p12.32 19432550 20145269 C10orf112chromosome 10 open reading 340895 427 428 frame 112 32 1010p12.32-p12.31 20145378 20609121 PLXDC2 plexin domain containing 284898 429 430 33 10 10q24.2 99905840 99994644 C10orf28 chromosome 10open reading 27291 431 432 frame 28 33 10 10q24 99997440 100017997 LOXL4lysyl oxidase-like 4 84171 433 434 33 10 10q24.2 100133314 100164931C10orf33 chromosome 10 open reading 84795 435 436 frame 33 33 1010q23.1-q23.3 100165646 100196694 HPS1 Hermansky-Pudlak syndrome 1 3257437, 439, 441, 443 438, 440, 442, 444 33 10 10q23-q24 100208865100985609 HPSE2 heparanase-2 60495 445 446 33 10 10q24.2 101080023101144077 CNNM1 cyclin M1 26507 447 448 33 10 10q24.1-q25.1 101146618101180336 GOT1 glutamic-oxaloacetic 2805 449 450 transaminase 1, soluble(aspartate aminotransferase 1) 34 11 11p15.3 11820141 11934691 USP47ubiquitin specific peptidase 47 55031 451 452 34 11 11p15.2 1194122911987493 DKK3 dickkopf homolog 3 (Xenopus 27122 453, 455, 457 454, 456,458 laevis) 34 11 11p15.3 12088714 12241908 MICAL2 microtubuleassociated 9645 459 460 monoxygenase, calponin and LIM domain containing2 35 11 11p15.1 19691488 20009720 NAV2 neuron navigator 2 89797 461, 463462, 464 35 11 11p15.1 20134336 20138446 DBX1 developing brain homeobox1 120237 465 466 36 11 11q21 95141766 95162429 FAM76B family withsequence similarity 143684 467 468 76, member B 36 11 11q21 9516329095204359 CEP57 centrosomal protein 57 kDa 9702 469 470 36 11 11q2295205694 95296920 MTMR2 myotubularin related protein 2 8898 471, 473,475 472, 474, 476 36 11 11q21 95351088 95715992 MAML2 mastermind-like 2(Drosophila) 84441 477 478 36 11 11q21 95725590 95762709 CCDC82hypothetical protein FLJ23518 79780 479 480 36 11 11q21 9576346295766375 JRKL jerky homolog-like (mouse) 8690 481 482 37 12 12q13.156114810 56130876 INHBC inhibin, beta C 3626 483 484 37 12 12q13.356135363 56138058 INHBE inhibin, beta E 83729 485 486 37 1212q13.2-q13.3 56140201 56152312 GLI1 glioma-associated oncogene 2735 487488 homolog 1 (zinc finger protein) 37 12 12q14 56152314 56168864ARHGAP9 Rho GTPase activating protein 9 64333 489 490 37 12 12q13.256168118 56196700 MARS methionine-tRNA synthetase 4141 491 492 37 1212q13.1-q13.2 56196643 56200567 DDIT3 DNA-damage-inducible transcript 31649 493 464 37 12 12q13.1-q13.2 56202926 56210198 MBD6 methyl-CpGbinding domain 114785 495 496 protein 6 37 12 12q13.2-q13.3 5621036156227245 DCTN2 dynactin 2 (p50) 10540 497 498 37 12 12q13.13 5623011456264821 KIF5A kinesin family member 5A 3798 499 500 37 12 12q13.356271324 56283298 PIP5K2C phosphatidylinositol-4-phosphate 79837 501 5025-kinase, type II, gamma 37 12 12q13.3 56284871 56289850 DTX3 deltex 3homolog (Drosophila) 196403 503 504 37 12 12q13.3 56291485 56297293 GEFTRAC/CDC42 exchange factor 115557 505, 507 506, 508 37 12 12q13 5630113556306201 SLC26A10 solute carrier family 26, member 65012 509, 511 510,512 10 37 12 12q13.3 56305945 56313251 B4GALNT1beta-1,4-N-acetyl-galactosaminyl 2583 513 514 transferase 1 37 1212q14.1 56312918 56355300 LOC441641 similar to ribosomal protein L13A441641 — — 37 12 12q13 56374187 56401606 OS9 amplified in osteosarcoma10956 515, 517, 519, 521 516, 518, 520, 522 37 12 12q14.1 5640526156422207 CENTG1 centaurin, gamma 1 116986 523 524 37 12 12q13.3 5642505156428293 TSPAN31 tetraspanin 31 6302 525 526 37 12 12q14 5642827056432431 CDK4 cyclin-dependent kinase 4 1019 527 528 37 12 12q14.156435167 56439466 MARCH9 membrane-associated ring finger 92979 529 530(C3HC4) 9 37 12 12q13.1-q13.3 56442386 56447151 CYP27B1 cytochrome P450,family 27, 1594 531 532 subfamily B, polypeptide 1 37 12 12q13 5644852356452522 METTL1 methyltransferase like 1 4234 533, 535, 537 534, 536,538 37 12 12q14.1 56452650 56462591 DKFZP586D0919hepatocellularcarcinoma- 25895 539, 541 540, 542 associated antigenHCA557a 37 12 12q13-q14 56462826 56476784 TSFM Ts translation elongationfactor, 10102 543 544 mitochondrial 37 12 12q14.1 56477710 56496119 AVILadvillin 10677 545 546 37 12 12q13-q15 56499977 56526789 CTDSP2 CTD(carboxy-terminal domain, 10106 547 548 RNA polymerase II, polypeptideA) small phosphatase 2 37 12 12q14.1 56621711 56637319 XRCC6BP1 XRCC6binding protein 1 91419 549 550 38 12 12q21.31 81254792 81375413C12orf26 chromosome 12 open reading 84190 551 552 frame 26 38 1212q21.31 81583402 82030533 TMTC2 transmembrane and 160335 553 554tetratricopeptide repeat containing 2 38 12 12q21.31 82047578 82048437LOC401725 similar to 60S ribosomal protein 401725 — — L6 (TAX-responsiveenhancer element binding protein 107) (TAXREB107) (Neoplasm-relatedprotein C140) 39 14 14q22.1 49620119 49629111 LOC196913 hypotheticalprotein LOC196913 196913 555 556 39 14 14q22.1 49645106 49653047C14orf138 chromosome 14 open reading 79609 557 558 frame 138 39 14 14q2149654812 49767751 SOS2 son of sevenless homolog 2 6655 559 560(Drosophila) 39 14 14q22.1 49782933 49848697 L2HGDH L-2-hydroxyglutarate79944 561 562 dehydrogenase 39 14 14q22.1 49848852 49859363 ATP5S ATPsynthase, H+ transporting, 27109 563, 565, 567 564, 566, 568mitochondrial F0 complex, subunit s (factor B) 39 14 14q22.1 4986647049932367 CDKL1 cyclin-dependent kinase-like 1 8814 569 570 (CDC2-relatedkinase) 39 14 14q11.2-q21 49954993 50069126 MAP4K5 mitogen-activatedprotein kinase 11183 571, 573 572, 574 kinase kinase kinane 5 39 1414q22.1 50096514 50169536 SPG3A spastic paraplegia 3A (autosomal 51062575, 577 576, 578 dominant) 39 14 14q22.1 50108247 50108634 SNRPGP smallnuclear ribonucleoprotein 326272 — — polypeptide G pseudogene 39 1414q13-q23 50170110 50204773 SAV1 salvador homolog 1 (Drosophila) 60485579 580 40 14 14q31.1 80358603 80359699 HMGN2P2 high-mobility groupnucleosomal 317726 — — binding domain 2 pseudogene 2 40 14 14q3180491679 80680525 TSHR thyroid stimulating hormone 7253 581, 583 582,584 receptor 40 14 14q31.1 80537231 80537914 RPL17P3 ribosomal proteinL17 326286 — — pseudogene 3 40 14 14q31.1 80568571 80569637 NMNATPnicotinamide nucleotide 326607 — — adenylyltransferase pseudogene 40 1414q31.1 80716147 80757328 GTF2A1 general transcription factor IIA, 1,2957 585, 587 586, 588 19/37 kDa 40 14 14q31.1 80782113 80782751LOC246720 dynein, cytoplasmic, light 246720 — — polypeptide pseudogene40 14 14q31.1 80794017 80796268 UNGP3 uracil-DNA glycosylase 319122 — —pseudogene 3 40 14 14q31.1 80806662 80934680 STON2 stonin 2 85439 589590 40 14 14q31.1 80868934 80869490 RPS24P3 ribosomal protein S24 326322— — pseudogene 3 41 16 16q12.1 46674385 46738182 ABCC12 ATP-bindingcassette, sub-family 94160 591 592 C (CFTR/MRP), member 12 41 16 16q12.146758323 46826589 ABCC11 ATP-binding cassette, sub-family 85320 593,595, 597 594, 596, 598 C (CFTR/MRP), member 11 41 16 16q12.1 4683571246944908 LONPL peroxisomal LON protease like 83752 599 600 41 16 16q1246951950 46954901 SIAH1 seven in absentia homolog 1 6477 601, 603 602,604 (Drosophila) 42 16 16q23.1 73590416 73702393 ZNRF1 zinc and ringfinger 1 84937 605 606 42 16 16q23.1 73703260 73708166 LDHD lactatedehydrogenase D 197257 607, 609 608, 610 42 16 16q23.1 73739926 73763486ZFP1 zinc finger protein 1 homolog 162239 611 612 (mouse) 42 16 16q23.173783375 73784646 LOC441774 similar to 40S ribosomal protein 441774 — —S4, Y isoform 1 42 16 16q23.1 73795499 73798582 CTRB2 chymotrypsinogenB2 440387 613 614 42 16 16q23-q24.1 73810399 73816322 CTRB1chymotrypsinogen B1 1504 615 616 42 16 16q22-q23 73820430 73843004 BCAR1breast cancer anti-estrogen 9564 617 618 resistance 1 42 1616q22.2-q22.3 73885116 74024860 CFDP1 craniofacial development protein 110428 619 620 42 16 16q23.1 74038426 74056085 LOC124491 LOC124491 124491621 622 42 16 16q22 74064526 74086428 CHST6 carbohydrate (N- 4166 623624 acetylglucosamine 6-O) sulfotransferase 6 43 16 16q23.1 7637998476571505 KIAA1576 KIAA1576 protein 57687 625 626 43 16 16q23 7661394676622239 CLEC3A C-type lectin domain family 3, 10143 627 628 member A 4316 16q23.1 76639896 76649857 LOC342419 similar to Keratin, type II342419 — — cytoskeletal 8 (Cytokeratin-8) (CK-8) (Keraton-8) (K8)(Cytokeratin endo A) 43 16 16q23.3-q24.1 76691052 77804065 WWOX WWdomain containing 51741 629, 631, 633 630, 632, 634 oxidoreductase 44 1818q11.2 22665430 22672683 LOC440489 ubiquitin fusion degradation 1-440489 — — like pseudogene 44 18 18q11.2-q12.1 22686008 22699699 AQP4aquaporin 4 361 635, 637 636, 638 44 18 18q11.2 22699270 22769908C18orf16 chromosome 18 open reading 147429 639 640 frame 16 44 1818q11.2 22749595 23019177 CHST9 carbohydrate (N- 83539 641 642acetylgalactosamine 4-0) sulfotransferase 9 44 18 18q11.2-q12.1 2317034123429126 FLJ45994 hypothetical gene supported by 400645 643 644 AK12788845 18 18q22-q23 61568468 61699155 CDH7 cadherin 7, type 2 1005 645, 647646, 648 46 22 22pter-q11.2|22q11.1 16449479 16486089 ATP6V1E1 ATPase,H+ transporting, 529 649, 651, 653 650, 652, 654 lysosomal 31 kDa, V1subunit E1 46 22 22q11 16496039 16586545 BCL2L13 BCL2-like 13 (apoptosis23786 655 656 facilitator) 46 22 22q11.1 16591460 16631812 BID BH3interacting domain death 637 657, 659, 661 658, 660, 662 agonist 46 2222q11.21 16697271 16764155 MICAL3 microtubule associated 57553 663, 665,667, 669, 671, 673 664, 666, 668, 670, 672, monoxygenase, calponin and674 LIM domain containing 3 46 22 22q11.21 16935243 16948790 PEX26peroxisome biogenesis factor 26 55670 675 676 46 22 22q11.1 1696811316989052 TUBA8 tubulin, alpha 8 51807 677 678 47 X Xq13.3 8017534580183261 NSBP1 nucleosomal binding protein 1 79366 679 680 47 X Xq13.380263767 80360191 SH3BGRL SH3 domain binding glutamic 6451 681 682acid-rich protein like

TABLE 5 List of additional longevity candidate genes from the genomewide and fine mapping association analyses derived from B36. In order toidentify genes not placed in the regions from Table 1 according to Build35, the region coordinates were converted to Build 36 using the UCSC(University of California Santa Cruz) online program LiftOver. Only newgenes that were mapped to this version of the genome asembly areincluded in this table. The first column corresponds to the regionidentifier provided in Table 1. The second and third columns correspondto the chromosome and cytogenetic band, respectively. The fourth andfifth columns correspond to the chromosomal start and end coordinates ofthe NCBI genome assembly derived from build 36 (the start and endposition relate to the + orientation of the NCBI assembly and do notnecessarily correspond to the orientation of the gene). The sixth andseventh columns correspond to the official gene symbol and gene name,respectively, and were obtained from the NCBI Entrez Gene database. Theeighth colums corresponds to the NCBI Entrez Gene Identifier (Gene ID).The ninth and tenth columns correspond to the Sequence IDs fromnucleotide (cDNA) and protein entries in the Sequence Listing. Start EndCytogenetic Position position Gene Entrez Region ID Chromosome Band B36B36 Symbol Gene Name Gene ID Nucleotide Seq ID Protein Seq ID 2 1 1q42227473294 227473969 HRES1 HTLV-1 related endogenous sequence 3272 683684 3 1 1q42.2 229223834 229226580 LOC644006 similar to RING fingerprotein 4 644006 685 686 4 1 1q43 238218704 238243181 LOC645884 similarto 40S ribosomal protein S7 (S8) 645884 — — 8 2 2q14.3 122156781122160491 LOC646151 hypothetical protein LOC646151 646151 — — 8 2 2q14.3122264517 122270396 LOC646179 hypothetical protein LOC646179 646179 687,689 688, 690 9 2 2q21.1 128033768 128111767 MYO7B myosin VIIB 4648 691692 12 3 3p26.1 7969498 8032988 LOC643291 hypothetical protein LOC643291643291 693, 695 694, 696 13 3 3p22.3 33106918 33113296 FLJ45032 similarto F40B5.2b 643853 697 698 14 3 3p22.1 40603030 40605031 LOC645807similar to ribosomal protein L5 645807 — — 15 4 4q21.1 77176927 77177241LOC643205 similar to ribosomal protein L36 643205 699 700 15 4 4q21.177189641 77210116 LOC643214 hypothetical protein LOC643214 643214 — — 164 4q23 101130917 101131660 LOC644721 similar to ribosomal proteinL7-like 1 644721 — — 17 4 4q28.1 126863872 126869392 LOC645841hypothetical protein LOC645841 645841 — — 19 6 6p22.1 27121856 27122077LOC645845 hypothetical protein LOC645845 645845 701 702 19 6 6p22.127358487 27374104 LOC645898 similar to SET domain and mariner 645898 — —transposase fusion gene 22 7 7p14.3 33732124 33734587 FLJ20712hypothetical protein FLJ20712 55025 703 704 23 7 7q32.1 127592525127595886 LOC646893 hypothetical protein LOC646893 646893 705, 707 706,708 23 7 7q32.1 127731406 127735094 MGC27345 hypothetical proteinMGC27345 157247 709 — 24 7 7q36.1 147908426 147908824 LOC402716 similarto 60S ribosomal protein L32 402716 710 711 24 7 7q36.1 147965225147965617 tcag7.1239 Rho guanine nucleotide exchange factor 643438 712,714 713, 715 (GEF) 5 25 7 7q36.1 149575240 149651741 LOC653722 similarto actin-related protein 3-beta 653722 716, 718 717, 719 25 7 7q36.1149941107 149942798 LOC643852 similar to transient receptor protein 6643852 — — 25 7 7q36.1 149953396 149960374 GIMAP6 GTPase, IMAP familymember 6 474344 720, 722 721, 723 25 7 7q36.1 150074898 150075786GIMAP3P GTPase, IMAP family member 3 474345 — — pseudogene 26 7 7q36.1150340233 150352519 ATG9B ATG9 autophagy related 9 homolog B 285973 724725 (S. cerevisiae) 26 7 7q36.1 150476609 150495800 LOC442747hypothetical LOC442747 442747 — — 26 7 7q36.1 150518899 150523589LOC392843 similar to IQ motif containing with AAA 392843 726, 728 727,729 domain 27 7 7q36.3 156926773 156956238 LOC393078 similar topolycystic kidney disease 1 like 3 393078 730 731 27 7 7q36.3 157346084157347702 LOC642620 hypothetical protein LOC642620 642620 732, 734 733,735 27 7 7q36.3 158205200 158205622 LOC645373 similar 60S ribosomalprotein L21 645373 736, 738 737, 739 31 9 9q32 112168283 112381975 SVEP1sushi, von Willebrand factor type A, EGF 79987 740, 742, 744, 746, 748,750 741, 743, 745, 747, 749, 751 and pentraxin domain containing 1 31 99q31.3 112774287 112840804 LOC644923 hypothetical protein LOC644923644923 752, 754 753, 755 34 11 11p15.1 11938953 11939310 RIG regulatedin glioma 10530 756 757 34 11 11p15.3 11939324 11939651 LOC644887hypothetical protein LOC644887 644887 758 759 36 11 11q21 9531193995336379 LOC643259 similar to Periphilin 1 (Gastric cancer 643259 — —antigen Ga50) 36 11 11q21 95627227 95630536 LOC645238 hypotheticalprotein LOC645238 645238 760, 762 761, 763 40 14 14q31.1 8003257780475637 C14orf145 chromosome 14 open reading frame 145 145508 764 76542 16 16q23.1 74086403 74105239 LOC645799 similar to carbohydrate (N-645799 766, 768 767, 769 acetylglucosamine 6-O) sulfotransferase 5 43 1616q23.1 76946927 76947232 LOC645947 similar to LSM3 homolog, U6 small645947 770 771 nuclear RNA associated 43 16 16q23.1 77416205 77417404LOC645957 similar to 40S ribosomal protein S3 645957 — — 45 18 18q22.161490076 61491640 LOC643448 hypothetical protein LOC643448 643448 772,774 773, 775 46 22 22q11.21 16697107 16864261 LOC642566 similar toProtein MICAL-3 642566 — —

TABLE 6 List of candidate genes based on EST clustering from the regionsidentified from the various Fine Mapping analyses. The first columncorresponds to the region identifier provided in Table 1. The secondcolumn corresponds to the chromosome number. The third and fourthcolumns correspond to the chromosomal start and end coordinates of theNCBI genome assemblies derived from build 35 (B35). The fifth columncorresponds to the ECGene Identifier, corresponding to the ECGene trackof UCSC. These ECGene entries were determined by their overlap with theregions from Table 1, based on the start and end coordinates of bothRegion and ECGene identifiers. The sixth and seventh columns correspondto the Sequence IDs from nucleotide and protein entries in the SequenceListing. Region ID Chromosome Start Position Build35 End PositionBuild35 Name Nucleotide Seq ID Protein Seq ID 1 1 172908440 172930123H1C20934.1 776 777 1 1 173163963 173256767 H1C20945.1 778 779 1 1173163963 173391998 H1C20945.2 780 781 1 1 173163963 173543625H1C20945.3 782 783 1 1 173254289 173258062 H1C20945.4 784 785 1 1173544405 173546396 H1C21012.1 786 787 1 1 173558095 173865681H1C21015.1 788 789 1 1 173561725 173882650 H1C21015.2 790 791 1 1173561856 173865681 H1C21015.3 792 793 1 1 173561856 173882650H1C21015.4 794 795 1 1 173569636 173865681 H1C21015.5 796 797 1 1173676847 173733572 H1C21015.6 798 799 2 1 225465254 225480756H1C26521.1 800 801 2 1 225535152 225562963 H1C26524.1 802 803 2 1225544148 225686077 H1C26525.1 804 805 2 1 225665942 225670044H1C26533.1 806 807 2 1 225711293 225713506 H1C26538.1 808 809 2 1225713543 225746488 H1C26539.1 810 811 2 1 225713543 225746889H1C26539.2 812 813 2 1 225713543 225747252 H1C26539.3 814 815 2 1225713543 225747986 H1C26539.4 816 817 2 1 225713556 225731364H1C26539.5 818 819 2 1 225713613 225746889 H1C26539.6 820 821 2 1225713613 225747986 H1C26539.7 822 823 2 1 225713791 225746488H1C26539.8 824 825 2 1 225714835 225716387 H1C26540.1 826 827 2 1225746082 225766672 H1C26553.1 828 829 2 1 225747015 225747983H1C26554.1 830 831 2 1 225761884 225765554 H1C26553.2 832 833 2 1225763487 225765652 H1C26557.1 834 835 2 1 225763487 225783115H1C26558.1 836 837 2 1 225763487 225785035 H1C26558.2 838 839 2 1225766021 225785776 H1C26558.3 840 841 2 1 225766949 225785776H1C26558.4 842 843 2 1 225873726 225874322 H1C26573.1 844 845 2 1225873726 225874662 H1C26574.1 846 847 2 1 225873726 225875353H1C26574.2 848 849 2 1 225873726 225875353 H1C26574.3 850 851 2 1225873726 225875364 H1C26574.4 852 853 2 1 225873726 225876576H1C26574.5 854 855 2 1 225873726 225876576 H1C26574.6 856 857 2 1225873726 225876580 H1C26574.7 858 859 2 1 225874360 225874800H1C26574.8 860 861 2 1 225874473 225874813 H1C26574.9 862 863 2 1225874473 225875364 H1C26574.10 864 865 2 1 225874825 225876579H1C26574.11 866 867 2 1 225882231 225883497 H1C26578.1 868 869 2 1225882231 225893233 H1C26579.1 870 871 2 1 225882231 225903212H1C26579.2 872 873 2 1 225882231 225950843 H1C26579.3 874 875 2 1225882231 225950843 H1C26579.4 876 877 2 1 225883770 225893233H1C26579.5 878 879 2 1 225883770 225903212 H1C26579.6 880 881 2 1225883770 225950843 H1C26579.7 882 883 2 1 225884183 225893233H1C26579.8 884 885 2 1 225884183 225903212 H1C26579.9 886 887 2 1225884222 225893233 H1C26579.10 888 889 2 1 225884222 225903212H1C26579.11 890 891 2 1 225884222 225950843 H1C26579.12 892 893 2 1225891416 225893552 H1C26579.13 894 895 2 1 225941635 225943988H1C26579.14 896 897 2 1 225950873 225956752 H1C26595.1 898 899 2 1225950965 225956746 H1C26595.2 900 901 2 1 225958993 225961481H1C26596.1 902 903 2 1 225958993 226001177 H1C26596.2 904 905 2 1225959064 225961481 H1C26596.3 906 907 2 1 225959064 226001177H1C26596.4 908 909 2 1 225959325 226001172 H1C26596.5 910 911 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32790208 H3C3250.2 1499 1500 13 3 32701683 32790371 H3C3250.31501 1502 13 3 32769496 32790208 H3C3250.5 1503 1504 13 3 3276949632790371 H3C3250.6 1505 1506 13 3 32769635 32777990 H3C3257.1 1507 150813 3 32780716 32790208 H3C3250.7 1509 1510 13 3 32780716 32790371H3C3250.8 1511 1512 13 3 32780808 32790370 H3C3263.1 1513 1514 13 332781038 32790208 H3C3250.9 1515 1516 13 3 32781038 32790371 H3C3250.101517 1518 13 3 32783184 32783942 H3C3265.1 1519 1520 13 3 3278603632790208 H3C3250.11 1521 1522 13 3 32786036 32790370 H3C3250.12 15231524 13 3 32788297 32790056 H3C3269.1 1525 1526 13 3 32797820 32802664H3C3273.1 1527 1528 13 3 32832844 32833862 H3C3276.1 1529 1530 13 332858710 32859454 H3C3278.1 1531 1532 13 3 32902381 32908307 H3C3284.11533 1534 13 3 32968043 32971404 H3C3289.1 1535 1536 13 3 3297760632991184 H3C3292.1 1537 1538 13 3 33013109 33113685 H3C3295.1 1539 154013 3 33013109 33113725 H3C3295.2 1541 1542 13 3 33013222 33113685H3C3295.3 1543 1544 13 3 33013222 33113725 H3C3295.4 1545 1546 13 333047194 33051082 H3C3295.5 1547 1548 13 3 33061808 33074768 H3C3295.61549 1550 13 3 33068471 33113725 H3C3295.7 1551 1552 13 3 3310691233108547 H3C3314.1 1553 1554 13 3 33106912 33113297 H3C3295.8 1555 155613 3 33106912 33113319 H3C3295.9 1557 1558 13 3 33123250 33125865H3C3315.1 1559 1560 13 3 33130452 33137102 H3C3316.1 1561 1562 13 333130452 33159686 H3C3316.2 1563 1564 13 3 33130452 33159686 H3C3316.31565 1566 13 3 33130452 33159686 H3C3316.4 1567 1568 13 3 3313045233160003 H3C3316.5 1569 1570 13 3 33130452 33160003 H3C3316.6 1571 157213 3 33130452 33160003 H3C3316.7 1573 1574 13 3 33130452 33160475H3C3316.10 1575 1576 13 3 33130452 33160475 H3C3316.8 1577 1578 13 333130452 33160475 H3C3316.9 1579 1580 13 3 33130517 33161533 H3C3317.11581 1582 13 3 33151243 33161533 H3C3322.1 1583 1584 13 3 3315888833159662 H3C3324.1 1585 1586 13 3 33158888 33159662 H3C3325.1 1587 158813 3 33158888 33160475 H3C3323.1 1589 1590 13 3 33158888 33160475H3C3324.2 1591 1592 13 3 33158888 33161533 H3C3326.1 1593 1594 13 333158888 33164269 H3C3326.2 1595 1596 13 3 33160599 33164266 H3C3328.11597 1598 13 3 33166537 33236511 H3C3331.1 1599 1600 13 3 3329393433403131 H3C3334.1 1601 1602 13 3 33293934 33403760 H3C3334.2 1603 160413 3 33294587 33295381 H3C3335.1 1605 1606 13 3 33328570 33348128H3C3336.1 1607 1608 14 3 39826154 40276813 H3C4013.1 1609 1610 14 339826154 40276813 H3C4013.2 1611 1612 14 3 39924391 39928519 H3C4017.11613 1614 14 3 40087615 40276813 H3C4013.3 1615 1616 14 3 4012187040316587 H3C4035.1 1617 1618 14 3 40183419 40208292 H3C4013.4 1619 162014 3 40189636 40326422 H3C4038.1 1621 1622 14 3 40242099 40325986H3C4038.2 1623 1624 14 3 40260923 40325973 H3C4038.3 1625 1626 14 340305621 40326422 H3C4038.4 1627 1628 14 3 40326178 40328894 H3C4055.11629 1630 14 3 40326178 40328919 H3C4055.2 1631 1632 14 3 4032618540328894 H3C4055.3 1633 1634 14 3 40327092 40328155 H3C4057.1 1635 163614 3 40403676 40445121 H3C4063.1 1637 1638 14 3 40407445 40469800H3C4065.1 1639 1640 14 3 40408205 40469675 H3C4065.2 1641 1642 14 340462026 40469824 H3C4065.3 1643 1644 14 3 40473818 40478867 H3C4077.11645 1646 14 3 40476386 40477767 H3C4080.1 1647 1648 14 3 4047786440478778 H3C4077.2 1649 1650 14 3 40477974 40478778 H3C4077.3 1651 165214 3 40477974 40478867 H3C4077.4 1653 1654 14 3 40478101 40478778H3C4077.5 1655 1656 14 3 40478101 40478867 H3C4077.6 1657 1658 14 340478444 40478778 H3C4082.1 1659 1660 14 3 40478444 40478863 H3C4082.21661 1662 14 3 40478870 40483649 H3C4083.1 1663 1664 14 3 4049364040504949 H3C4086.1 1665 1666 14 3 40498393 40523951 H3C4087.1 1667 166814 3 40498397 40503589 H3C4086.2 1669 1670 14 3 40505070 40506732H3C4088.1 1671 1672 14 3 40507326 40508017 H3C4089.1 1673 1674 14 340522486 40534051 H3C4087.2 1675 1676 14 3 40522534 40534201 H3C4087.31677 1678 14 3 40541372 40550438 H3C4094.1 1679 1680 14 3 4054139240549344 H3C4094.2 1681 1682 14 3 40541392 40549344 H3C4094.3 1683 168414 3 40541392 40556021 H3C4094.4 1685 1686 14 3 40541392 40591182H3C4094.5 1687 1688 14 3 40541500 40549344 H3C4094.6 1689 1690 14 340601956 40632304 H3C4106.1 1691 1692 14 3 40619627 40621922 H3C4107.11693 1694 15 4 77006937 77079451 H4C6331.1 1695 1696 15 4 7700693777092543 H4C6331.2 1697 1698 15 4 77006937 77092620 H4C6331.3 1699 170015 4 77006937 77092620 H4C6331.4 1701 1702 15 4 77101353 77103878H4C6353.1 1703 1704 15 4 77102701 77103878 H4C6353.2 1705 1706 15 477137568 77138968 H4C6355.1 1707 1708 15 4 77138195 77148180 H4C6356.11709 1710 15 4 77138197 77144325 H4C6357.1 1711 1712 15 4 7713819777180903 H4C6358.1 1713 1714 15 4 77138197 77180903 H4C6358.2 1715 171615 4 77138197 77180903 H4C6358.3 1717 1718 15 4 77161280 77161849H4C6358.4 1719 1720 15 4 77166632 77168374 H4C6358.5 1721 1722 15 477189594 77199073 H4C6366.1 1723 1724 15 4 77195814 77219345 H4C6365.11725 1726 15 4 77228243 77269292 H4C6374.1 1727 1728 15 4 7725176777252730 H4C6383.1 1729 1730 15 4 77259121 77284271 H4C6387.1 1731 173215 4 77279674 77285841 H4C6391.1 1733 1734 15 4 77279674 77289473H4C6391.2 1735 1736 15 4 77279801 77285841 H4C6391.3 1737 1738 15 477279801 77289473 H4C6391.4 1739 1740 15 4 77289531 77391124 H4C6393.11741 1742 15 4 77299447 77301868 H4C6395.1 1743 1744 15 4 7731202077314529 H4C6397.1 1745 1746 15 4 77312020 77319747 H4C6397.2 1747 174815 4 77312129 77319747 H4C6397.3 1749 1750 15 4 77353024 77391071H4C6393.2 1751 1752 15 4 77353024 77391124 H4C6393.3 1753 1754 15 477353041 77357441 H4C6393.4 1755 1756 15 4 77353079 77391124 H4C6393.51757 1758 15 4 77385547 77391124 H4C6393.6 1759 1760 15 4 7739286577396107 H4C6409.1 1761 1762 15 4 77392865 77396774 H4C6409.2 1763 176415 4 77392865 77426834 H4C6409.3 1765 1766 15 4 77392992 77396774H4C6409.4 1767 1768 15 4 77392992 77409744 H4C6409.5 1769 1770 15 477392992 77426778 H4C6409.6 1771 1772 15 4 77392992 77426834 H4C6409.71773 1774 15 4 77393159 77426834 H4C6409.8 1775 1776 15 4 7739320077396107 H4C6409.9 1777 1778 15 4 77393200 77396774 H4C6409.10 1779 178015 4 77437059 77439737 H4C6422.1 1781 1782 15 4 77437059 77492258H4C6423.1 1783 1784 15 4 77439785 77492258 H4C6423.2 1785 1786 15 477440597 77441508 H4C6425.1 1787 1788 15 4 77457228 77462054 H4C6437.11789 1790 15 4 77457699 77492258 H4C6423.3 1791 1792 15 4 7745792077492258 H4C6423.4 1793 1794 15 4 77461862 77462675 H4C6439.1 1795 179615 4 77492371 77562112 H4C6443.1 1797 1798 15 4 77530052 77589462H4C6443.2 1799 1800 15 4 77546991 77550268 H4C6443.3 1801 1802 15 477557517 77562106 H4C6443.4 1803 1804 16 4 100853158 100862526 H4C8545.11805 1806 16 4 100853158 100902332 H4C8545.2 1807 1808 16 4 100914864100933921 H4C8552.1 1809 1810 16 4 101095161 101139960 H4C8556.1 18111812 16 4 101095161 101147036 H4C8556.2 1813 1814 16 4 101095161101147945 H4C8556.3 1815 1816 16 4 101095161 101148522 H4C8556.4 18171818 16 4 101095161 101148522 H4C8556.5 1819 1820 16 4 101142598101148522 H4C8556.6 1821 1822 16 4 101156672 101172881 H4C8566.1 18231824 16 4 101156672 101172881 H4C8566.2 1825 1826 16 4 101156724101172881 H4C8566.3 1827 1828 16 4 101156724 101172881 H4C8566.4 18291830 16 4 101159540 101172881 H4C8566.5 1831 1832 16 4 101159540101172881 H4C8566.6 1833 1834 16 4 101174582 101177015 H4C8571.1 18351836 16 4 101174582 101184927 H4C8572.1 1837 1838 16 4 101174582101205426 H4C8572.2 1839 1840 16 4 101174582 101224923 H4C8572.3 18411842 16 4 101174582 101224968 H4C8572.4 1843 1844 16 4 101177881101184927 H4C8572.5 1845 1846 16 4 101178857 101224968 H4C8572.6 18471848 16 4 101200817 101224968 H4C8572.7 1849 1850 16 4 101201588101224968 H4C8572.8 1851 1852 16 4 101202408 101224921 H4C8572.9 18531854 16 4 101226393 101228723 H4C8588.1 1855 1856 16 4 101226408101228094 H4C8588.2 1857 1858 16 4 101226408 101228592 H4C8588.3 18591860 16 4 101226408 101228634 H4C8588.4 1861 1862 16 4 101226408101228634 H4C8588.5 1863 1864 16 4 101226408 101228690 H4C8588.6 18651866 16 4 101226408 101228716 H4C8588.7 1867 1868 16 4 101226419101228690 H4C8588.8 1869 1870 16 4 101227201 101228690 H4C8588.9 18711872 16 4 101228340 101244660 H4C8590.1 1873 1874 16 4 101228828101316148 H4C8593.1 1875 1876 17 4 126595171 126601376 H4C10553.1 18771878 17 4 126731474 126771691 H4C10567.1 1879 1880 17 4 126768932126771686 H4C10570.1 1881 1882 17 4 126955477 127031553 H4C10572.1 18831884 18 4 143305522 143752172 H4C11432.1 1885 1886 18 4 143305522143753027 H4C11432.2 1887 1888 18 4 143305522 143753166 H4C11432.3 18891890 18 4 143305522 143793029 H4C11432.4 1891 1892 18 4 143305522143839425 H4C11432.5 1893 1894 18 4 143305522 144004658 H4C11432.6 18951896 18 4 143307483 143752172 H4C11432.7 1897 1898 18 4 143307483143753027 H4C11432.8 1899 1900 18 4 143307483 143753166 H4C11432.9 19011902 18 4 143307483 143793029 H4C11432.10 1903 1904 18 4 143307483143839425 H4C11432.11 1905 1906 18 4 143307483 144004658 H4C11432.121907 1908 18 4 143471856 144004661 H4C11432.13 1909 1910 18 4 143676169143684210 H4C11432.14 1911 1912 18 4 143705240 143710242 H4C11432.151913 1914 18 4 143705240 143753166 H4C11432.16 1915 1916 18 4 143793203143797745 H4C11496.1 1917 1918 18 4 143844774 143939708 H4C11503.1 19191920 18 4 143933269 144045577 H4C11432.17 1921 1922 19 6 2703274927050124 H6C3386.1 1923 1924 19 6 27095691 27099155 H6C3392.1 1925 192619 6 27096039 27099682 H6C3392.2 1927 1928 19 6 27167207 27202211H6C3396.1 1929 1930 19 6 27199620 27208551 H6C3401.1 1931 1932 19 627201837 27208519 H6C3401.2 1933 1934 19 6 27201837 27208519 H6C3401.31935 1936 19 6 27201837 27208520 H6C3401.4 1937 1938 19 6 2720183727208534 H6C3401.5 1939 1940 19 6 27203224 27208520 H6C3401.6 1941 194219 6 27207746 27208554 H6C3406.1 1943 1944 19 6 27208799 27211049H6C3407.1 1945 1946 19 6 27213342 27222611 H6C3409.1 1947 1948 19 627214051 27222611 H6C3409.2 1949 1950 19 6 27215039 27216264 H6C3411.11951 1952 19 6 27217851 27221754 H6C3413.1 1953 1954 19 6 2722286327223325 H6C3415.1 1955 1956 19 6 27323458 27332228 H6C3422.1 1957 195819 6 27323458 27332377 H6C3422.2 1959 1960 19 6 27323486 27332228H6C3422.3 1961 1962 19 6 27323486 27332377 H6C3422.4 1963 1964 19 627326828 27332228 H6C3422.5 1965 1966 19 6 27326828 27332377 H6C3422.61967 1968 19 6 27327079 27332228 H6C3422.7 1969 1970 19 6 2732707927332377 H6C3422.8 1971 1972 19 6 27327389 27331298 H6C3422.9 1973 197419 6 27327389 27332228 H6C3422.10 1975 1976 19 6 27327389 27332377H6C3422.11 1977 1978 19 6 27328640 27332228 H6C3422.12 1979 1980 19 627328640 27332382 H6C3422.13 1981 1982 19 6 27361660 27387070 H6C3426.11983 1984 19 6 27400518 27401721 H6C3429.1 1985 1986 19 6 2743357727439398 H6C3430.1 1987 1988 19 6 27433577 27447283 H6C3430.2 1989 199019 6 27433581 27435982 H6C3431.1 1991 1992 19 6 27438562 27446616H6C3430.3 1993 1994 19 6 27450392 27477326 H6C3436.1 1995 1996 20 6152220799 152517103 H6C15161.1 1997 1998 20 6 152220927 152512543H6C15161.3 1999 2000 20 6 152220927 152512543 H6C15161.4 2001 2002 20 6152220927 152512543 H6C15161.5 2003 2004 20 6 152222355 152542850H6C15161.7 2005 2006 20 6 152534932 152545465 H6C15198.1 2007 2008 20 6152534932 152561012 H6C15199.1 2009 2010 20 6 152534932 152581536H6C15199.2 2011 2012 20 6 152534932 152592715 H6C15199.3 2013 2014 20 6152534932 152724416 H6C15199.4 2015 2016 20 6 152534932 152731593H6C15199.5 2017 2018 20 6 152534932 152731628 H6C15199.6 2019 2020 20 6152534932 153050100 H6C15199.7 2021 2022 20 6 152534932 153050648H6C15199.8 2023 2024 20 6 152535006 152581536 H6C15199.9 2025 2026 20 6152535006 152592715 H6C15199.10 2027 2028 20 6 152535006 152637892H6C15199.11 2029 2030 20 6 152535006 152724416 H6C15199.12 2031 2032 206 152535006 152731593 H6C15199.13 2033 2034 20 6 152535006 153050648H6C15199.14 2035 2036 20 6 152691351 152695777 H6C15199.15 2037 2038 206 152728470 152747437 H6C15199.16 2039 2040 20 6 152748527 152794613H6C15199.17 2041 2042 20 6 152780241 152794613 H6C15199.18 2043 2044 206 152793778 152794959 H6C15254.1 2045 2046 20 6 152815646 152818211H6C15256.1 2047 2048 20 6 152959180 152960210 H6C15265.1 2049 2050 20 6153096548 153102408 H6C15273.1 2051 2052 20 6 153111144 153137827H6C15274.1 2053 2054 20 6 153111144 153137967 H6C15274.2 2055 2056 20 6153111149 153137827 H6C15274.3 2057 2058 20 6 153111194 153137827H6C15274.4 2059 2060 20 6 153111194 153137967 H6C15274.5 2061 2062 20 6153126913 153135896 H6C15274.6 2063 2064 20 6 153164045 153173012H6C15280.1 2065 2066 20 6 153207744 153245162 H6C15281.1 2067 2068 20 6153218001 153218372 H6C15281.2 2069 2070 20 6 153276832 153281697H6C15285.1 2071 2072 21 7 30327977 30339064 H7C3483.1 2073 2074 21 730329167 30375681 H7C3483.2 2075 2076 21 7 30338586 30346469 H7C3490.12077 2078 21 7 30362655 30364587 H7C3501.1 2079 2080 21 7 3036317530365076 H7C3483.3 2081 2082 21 7 30363175 30390615 H7C3483.4 2083 208421 7 30370951 30385944 H7C3483.5 2085 2086 21 7 30372848 30377751H7C3483.6 2087 2088 21 7 30374264 30376806 H7C3507.1 2089 2090 21 730379259 30398671 H7C3483.7 2091 2092 21 7 30381343 30387531 H7C3483.82093 2094 21 7 30387163 30398671 H7C3483.9 2095 2096 21 7 3038750430390635 H7C3483.10 2097 2098 21 7 30387559 30407664 H7C3483.11 20992100 21 7 30390378 30398828 H7C3483.12 2101 2102 21 7 30407420 30446889H7C3515.1 2103 2104 21 7 30407703 30446889 H7C3515.2 2105 2106 21 730427111 30434534 H7C3515.3 2107 2108 21 7 30443369 30446889 H7C3515.42109 2110 21 7 30444307 30446889 H7C3515.5 2111 2112 21 7 3046543930495298 H7C3529.1 2113 2114 21 7 30466102 30512959 H7C3529.2 2115 211621 7 30495075 30512901 H7C3529.3 2117 2118 21 7 30510913 30558570H7C3543.1 2119 2120 21 7 30564990 30570458 H7C3546.1 2121 2122 21 730584272 30652169 H7C3546.2 2123 2124 21 7 30584276 30705241 H7C3546.32125 2126 21 7 30592389 30599511 H7C3546.4 2127 2128 21 7 3060954630616266 H7C3552.1 2129 2130 21 7 30665897 30685633 H7C3546.5 2131 213221 7 30669774 30705241 H7C3546.6 2133 2134 21 7 30669774 30705647H7C3546.7 2135 2136 21 7 30724707 30738371 H7C3564.1 2137 2138 21 730724707 30738373 H7C3564.2 2139 2140 21 7 30724707 30738373 H7C3564.32141 2142 21 7 30776875 30792381 H7C3568.1 2143 2144 21 7 3078238730792374 H7C3568.2 2145 2146 21 7 30782387 30792374 H7C3568.3 2147 214821 7 30782387 30792374 H7C3568.4 2149 2150 21 7 30782387 30792374H7C3568.5 2151 2152 21 7 30784823 30798071 H7C3568.6 2153 2154 21 730784883 30788813 H7C3568.7 2155 2156 21 7 30865381 30920152 H7C3574.12157 2158 21 7 30921868 30924333 H7C3574.2 2159 2160 21 7 3092186830924569 H7C3574.3 2161 2162 22 7 32308387 32852274 H7C3646.3 2163 216422 7 32680443 32704646 H7C3724.2 2165 2166 22 7 32681014 32704646H7C3724.4 2167 2168 22 7 32681851 32704646 H7C3724.6 2169 2170 22 732694934 32699868 H7C3730.1 2171 2172 22 7 32698777 32701069 H7C3733.12173 2174 22 7 32725706 32726984 H7C3737.1 2175 2176 22 7 3272846832735044 H7C3739.1 2177 2178 22 7 32728468 32755815 H7C3739.2 2179 218022 7 32728468 32755858 H7C3739.3 2181 2182 22 7 32728468 32755910H7C3739.4 2183 2184 22 7 32728468 32756016 H7C3739.5 2185 2186 22 732728468 32756020 H7C3739.6 2187 2188 22 7 32728468 32756028 H7C3739.72189 2190 22 7 32728468 32756036 H7C3739.8 2191 2192 22 7 3272846832756289 H7C3739.9 2193 2194 22 7 32729662 32735044 H7C3739.10 2195 219622 7 32729662 32755815 H7C3739.11 2197 2198 22 7 32729662 32755910H7C3739.12 2199 2200 22 7 32729662 32756016 H7C3739.13 2201 2202 22 732729662 32756020 H7C3739.14 2203 2204 22 7 32729662 32756028 H7C3739.152205 2206 22 7 32729662 32756036 H7C3739.16 2207 2208 22 7 3272966232756289 H7C3739.17 2209 2210 22 7 32729666 32755858 H7C3739.18 22112212 22 7 32734189 32756036 H7C3739.19 2213 2214 22 7 32770264 32819783H7C3747.1 2215 2216 22 7 32791314 32792420 H7C3748.1 2217 2218 22 732818036 32819779 H7C3762.1 2219 2220 22 7 32818081 32819780 H7C3763.12221 — 22 7 32826965 32830487 H7C3766.1 2222 2223 22 7 32826965 32853758H7C3766.2 2224 2225 22 7 32826965 32875646 H7C3766.3 2226 2227 22 732826965 32875646 H7C3766.4 2228 2229 22 7 32830290 32834064 H7C3770.12230 2231 22 7 32876051 32908369 H7C3788.1 2232 2233 22 7 3290764832926257 H7C3791.1 2234 2235 22 7 32907650 32910458 H7C3791.2 2236 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22658070 H18C2360.1 4489 4490 44 1822686000 22687520 H18C2365.1 4491 4492 44 18 22686000 22696135H18C2366.1 4493 4494 44 18 22686000 22696810 H18C2366.2 4495 4496 44 1822686000 22696810 H18C2366.3 4497 4498 44 18 22686000 22699747H18C2366.4 4499 4500 44 18 22686000 22699747 H18C2366.5 4501 4502 44 1822687947 22696135 H18C2366.6 4503 4504 44 18 22687947 22696810H18C2366.7 4505 4506 44 18 22687947 22699747 H18C2366.8 4507 4508 44 1822687947 22699747 H18C2366.9 4509 4510 44 18 22699266 23024648H18C2369.1 4511 4512 44 18 22699269 22769908 H18C2369.2 4513 4514 44 1822749592 22976969 H18C2374.1 4515 4516 44 18 22749592 23019305H18C2374.2 4517 4518 44 18 22749592 23019305 H18C2374.3 4519 4520 44 1822749736 22976969 H18C2374.4 4521 4522 44 18 22749736 23019305H18C2374.5 4523 4524 44 18 22749736 23019305 H18C2374.6 4525 4526 44 1822750122 23019305 H18C2374.7 4527 4528 44 18 22750122 23019305H18C2374.8 4529 4530 44 18 23170340 23429126 H18C2403.1 4531 4532 44 1823170340 23429126 H18C2403.2 4533 4534 44 18 23170701 23226829H18C2403.3 4535 4536 44 18 23231913 23235865 H18C2406.1 4537 4538 45 1861568467 61699155 H18C5401.1 4539 4540 45 18 61568867 61682340H18C5401.2 4541 4542 45 18 61568867 61699154 H18C5401.3 4543 4544 45 1861735535 61740532 H18C5415.1 4545 4546 46 22 16449458 16486067 H22C214.14547 4548 46 22 16449458 16486127 H22C214.2 4549 4550 46 22 1644945816486142 H22C214.3 4551 4552 46 22 16449478 16452050 H22C214.4 4553 455446 22 16449478 16486142 H22C214.5 4555 4556 46 22 16449479 16486067H22C214.6 4557 4558 46 22 16449479 16486127 H22C214.7 4559 4560 46 2216449479 16486142 H22C214.8 4561 4562 46 22 16449479 16486142 H22C214.94563 4564 46 22 16449785 16486067 H22C214.10 4565 4566 46 22 1644978516486127 H22C214.11 4567 4568 46 22 16449785 16486142 H22C214.12 45694570 46 22 16451878 16486127 H22C214.13 4571 4572 46 22 1645812816486127 H22C214.14 4573 4574 46 22 16486235 16559740 H22C222.1 45754576 46 22 16495909 16561098 H22C222.2 4577 4578 46 22 16495909 16561098H22C222.3 4579 4580 46 22 16495909 16586456 H22C222.4 4581 4582 46 2216495909 16586456 H22C222.5 4583 4584 46 22 16495909 16586456 H22C222.64585 4586 46 22 16495909 16586456 H22C222.7 4587 4588 46 22 1649590916586546 H22C222.10 4589 4590 46 22 16495909 16586546 H22C222.11 45914592 46 22 16495909 16586546 H22C222.8 4593 4594 46 22 16495909 16586546H22C222.9 4595 4596 46 22 16495909 16587940 H22C222.12 4597 4598 46 2216495909 16587940 H22C222.13 4599 4600 46 22 16495909 16587940H22C222.14 4601 4602 46 22 16495909 16587940 H22C222.15 4603 4604 46 2216495909 16588231 H22C222.16 4605 4606 46 22 16495909 16588231H22C222.17 4607 4608 46 22 16495909 16588231 H22C222.18 4609 4610 46 2216495909 16588231 H22C222.19 4611 4612 46 22 16496095 16586456H22C222.20 4613 4614 46 22 16496095 16586546 H22C222.21 4615 4616 46 2216496095 16587940 H22C222.22 4617 4618 46 22 16496095 16588231H22C222.23 4619 4620 46 22 16564029 16586456 H22C222.24 4621 4622 46 2216564029 16586546 H22C222.25 4623 4624 46 22 16564029 16587940H22C222.26 4625 4626 46 22 16564029 16588231 H22C222.27 4627 4628 46 2216570932 16574491 H22C238.1 4629 4630 46 22 16583995 16588231 H22C242.14631 4632 46 22 16591336 16592839 H22C246.1 4633 4634 46 22 1659140516595471 H22C247.1 4635 4636 46 22 16591405 16631303 H22C247.2 4637 463846 22 16591405 16631787 H22C247.3 4639 4640 46 22 16591405 16631806H22C247.4 4641 4642 46 22 16591405 16631815 H22C247.5 4643 4644 46 2216591405 16631815 H22C247.6 4645 4646 46 22 16591405 16631815 H22C247.74647 4648 46 22 16591458 16595471 H22C247.8 4649 4650 46 22 1659145816631333 H22C247.9 4651 4652 46 22 16591458 16631787 H22C247.10 46534654 46 22 16591458 16631806 H22C247.11 4655 4656 46 22 1659145816631815 H22C247.12 4657 4658 46 22 16591458 16631815 H22C247.13 46594660 46 22 16591458 16631815 H22C247.14 4661 4662 46 22 1659146016607495 H22C247.15 4663 4664 46 22 16591460 16631815 H22C247.16 46654666 46 22 16592531 16598514 H22C247.17 4667 4668 46 22 1659253116631303 H22C247.18 4669 4670 46 22 16592531 16631815 H22C247.19 46714672 46 22 16592531 16631815 H22C247.20 4673 4674 46 22 1659259016631815 H22C247.21 4675 4676 46 22 16592693 16631303 H22C247.22 46774678 46 22 16592693 16631787 H22C247.23 4679 4680 46 22 1659269316631815 H22C247.24 4681 4682 46 22 16592693 16631815 H22C247.25 46834684 46 22 16592696 16601297 H22C247.26 4685 4686 46 22 1659269616631815 H22C247.27 4687 4688 46 22 16634681 16636800 H22C261.1 46894690 46 22 16637180 16637883 H22C262.1 4691 4692 46 22 16644967 16669095H22C263.1 4693 4694 46 22 16644967 16685103 H22C263.2 4695 4696 46 2216644968 16646266 H22C264.1 4697 4698 46 22 16644968 16647610 H22C266.14699 4700 46 22 16648351 16675316 H22C263.3 4701 4702 46 22 1665599316657308 H22C272.1 4703 4704 46 22 16656011 16657390 H22C273.1 4705 470646 22 16676183 16689247 H22C263.4 4707 4708 46 22 16678762 16679688H22C263.5 4709 4710 46 22 16691654 16764155 H22C292.1 4711 4712 46 2216691657 16764155 H22C292.2 4713 4714 46 22 16696299 16858821 H22C292.34715 4716 46 22 16709476 16720104 H22C298.1 4717 4718 46 22 1675256016754105 H22C292.4 4719 4720 46 22 16756401 16763982 H22C292.5 4721 472246 22 16861604 16864628 H22C349.1 4723 4724 46 22 16886704 16895288H22C352.1 4725 4726 46 22 16933846 16935007 H22C354.1 4727 4728 46 2216933846 16935117 H22C353.1 4729 4730 46 22 16935239 16948350 H22C356.14731 4732 46 22 16935242 16948790 H22C356.2 4733 4734 46 22 1693525816945781 H22C356.3 4735 4736 46 22 16935313 16945779 H22C356.4 4737 473846 22 16940952 16948350 H22C357.1 4739 4740 46 22 16940952 16948759H22C357.2 4741 4742 46 22 16942483 16945936 H22C356.5 4743 4744 46 2216945214 16948350 H22C359.1 4745 4746 46 22 16945214 16948759 H22C359.24747 4748 46 22 16945533 16950882 H22C360.1 4749 4750 46 22 1694628916950882 H22C361.1 4751 4752 46 22 16946470 16948759 H22C362.1 4753 475446 22 16947053 16948759 H22C357.3 4755 4756 46 22 16947053 16948759H22C364.1 4757 4758 46 22 16947109 16948350 H22C357.4 4759 4760 46 2216967795 16988476 H22C371.1 4761 4762 46 22 16967795 16989052 H22C371.24763 4764 46 22 16978427 16988428 H22C371.3 4765 4766 47 X 8017534480263530 HXC4994.1 4767 4768 47 X 80175344 80263557 HXC4994.2 4769 477047 X 80175344 80263586 HXC4994.3 4771 4772 47 X 80175647 80180122HXC4994.4 4773 4774 47 X 80175709 80180122 HXC4994.5 4775 4776 47 X80175709 80263586 HXC4994.6 4777 4778 47 X 80263587 80360191 HXC4997.14779 4780 47 X 80263621 80359238 HXC4997.2 4781 4782 47 X 8026362180360191 HXC4997.3 4783 4784 47 X 80263661 80360191 HXC4997.4 4785 478647 X 80263715 80359238 HXC4997.5 4787 4788 47 X 80263862 80359238HXC4997.6 4789 4790 47 X 80263862 80360191 HXC4997.7 4791 4792 47 X80264244 80359238 HXC4997.8 4793 4794 47 X 80264244 80360191 HXC4997.94795 4796 47 X 80273145 80359238 HXC4997.10 4797 4798 47 X 8027314580360191 HXC4997.11 4799 4800 47 X 80317137 80360191 HXC4997.12 48014802 47 X 80356945 80358119 HXC5008.1 4803 4804

TABLE 7 List of significantly associated haplotypes based on thelongevity fine mapping results using the Quebec Founder Population(QFP). Individual haplotypes with associated relative risks arepresented in each row of the table; these values were extracted from theassociated marker haplotype window with the most significant p value foreach SNP in Table 3. The first column lists the region ID as presentedin Table 1. The Haplotype column lists the specific nucleotides for theindividual SNP alleles contributing to the haplotype reported. The Caseand Control columns correspond to the numbers of cases and controls,respectively, containing the haplotype variant noted in the Haplotypecolumn. The Total Case and Total Control columns list the total numbersof cases and controls for which genotype data was available for thehaplotype in question, The RR column gives the relative risk for eachparticular haplotype. The remainder of the columns lists the SeqIDs forthe SNPs contributing to the haplotype and their relative location withrespect to the central marker. The Central marker (0) column lists theSeqID for the central marker on which the haplotype is based. Flankingmarkers are identified by minus (−) or plus (+) signs to indicatelocation of flanking SNPs. Re- Total % Central Central Central CentralCentral Central Central Central Central Central Central gion Con- TotalCon- % Con- marker marker marker marker marker marker marker markermarker marker marker ID Haplotype Case trol Case trol Case trol RR (−5)(−4) (−3) (−2) (−1) (0) (+1) (+2) (+3) (+4) (+5) 1 CTTG 51 81 1258 12620.04 0.06 0.62 8357 4850 4853 8358 4858 1 A 820 767 1258 1262 0.65 0.611.21 4858 1 G 438 495 1258 1262 0.35 0.39 0.83 4858 1 GAT 125 166 12581262 0.10 0.13 0.73 4858 4863 8359 2 A 855 799 1272 1266 0.67 0.63 1.204917 2 T 417 467 1272 1266 0.33 0.37 0.83 4917 2 TCG 2 11 1272 1266 0.000.01 0.18 4917 8360 8361 3 ACCGG 27 11 1270 1266 0.02 0.01 2.48 83624971 4972 4973 4975 3 CGG 30 11 1270 1266 0.02 0.01 2.76 4972 4973 49753 A 1178 1214 1270 1266 0.93 0.96 0.55 4975 3 G 92 52 1270 1266 0.070.04 1.82 4975 3 GGG 91 52 1270 1266 0.07 0.04 1.80 4975 4977 8363 3AAGTT 107 137 1270 1266 0.08 0.11 0.76 4975 4977 8363 4978 4980 3 GGGTG89 51 1270 1266 0.07 0.04 1.80 4975 4977 8363 4978 4980 6 T 871 930 12661260 0.69 0.74 0.78 5118 6 C 395 330 1266 1260 0.31 0.26 1.28 5118 6 CAG382 315 1266 1260 0.30 0.25 1.30 5118 5119 8375 6 A 553 617 1266 12600.44 0.49 0.81 8375 6 G 713 643 1266 1260 0.56 0.51 1.24 8375 6 T 609683 1266 1260 0.48 0.54 0.78 5120 6 C 657 577 1266 1260 0.52 0.46 1.285120 6 C 403 470 1266 1260 0.32 0.37 0.78 5122 6 T 863 790 1266 12600.68 0.63 1.27 5122 6 C 385 462 1266 1260 0.30 0.37 0.75 5123 6 A 881798 1266 1260 0.70 0.63 1.32 5123 6 AAG 612 544 1266 1260 0.48 0.43 1.235123 5124 8376 6 CAG 200 247 1266 1260 0.16 0.20 0.77 5123 5124 8376 6AAGCT 490 429 1266 1260 0.39 0.34 1.22 5123 5124 8376 5127 8377 6 CAGTC123 162 1266 1260 0.10 0.13 0.73 5123 5124 8376 5127 8377 6 T 419 4661266 1260 0.33 0.37 0.84 5127 6 C 847 794 1266 1260 0.67 0.63 1.19 51276 C 435 482 1266 1260 0.34 0.38 0.84 8377 6 T 831 778 1266 1260 0.660.62 1.18 8377 7 T 1112 1146 1268 1264 0.88 0.91 0.73 5240 7 C 156 1181268 1264 0.12 0.09 1.36 5240 7 A 1099 1130 1268 1264 0.87 0.89 0.775241 7 C 169 134 1268 1264 0.13 0.11 1.30 5241 8 CTAGA 257 308 1262 12600.20 0.24 0.79 5309 5312 5313 8382 8383 8 AGG 863 803 1262 1260 0.680.64 1.23 5313 8382 8383 8 AGA 257 308 1262 1260 0.20 0.24 0.79 53138382 8383 8 G 863 803 1262 1260 0.68 0.64 1.23 8383 8 A 399 457 12621260 0.32 0.36 0.81 8383 8 AAC 221 265 1262 1260 0.18 0.21 0.80 83838384 8385 9 GGA 336 287 1270 1262 0.26 0.23 1.22 5370 5371 5372 9 ATG407 470 1270 1262 0.32 0.37 0.79 5370 5371 5372 9 A 861 789 1270 12620.68 0.63 1.26 5372 9 G 409 473 1270 1262 0.32 0.37 0.79 5372 9 ATA 489426 1270 1262 0.39 0.34 1.23 5372 5374 8387 9 GTA 408 473 1270 1262 0.320.37 0.79 5372 5374 8387 10 CGC 368 421 1266 1254 0.29 0.34 0.81 83885442 5443 10 CAT 538 478 1266 1254 0.42 0.38 1.20 8388 5442 5443 10 CGC714 756 1266 1254 0.56 0.60 0.85 5442 5443 5444 10 ATT 511 435 1266 12540.40 0.35 1.27 5442 5443 5444 10 ATG 27 43 1266 1254 0.02 0.03 0.61 54425443 5444 10 CGCGGAC 365 419 1266 1254 0.29 0.33 0.81 8388 5442 54435444 5445 5446 5448 10 CATTACT 472 387 1266 1254 0.37 0.31 1.33 83885442 5443 5444 5445 5446 5448 10 CATGGAC 27 43 1266 1254 0.02 0.03 0.618388 5442 5443 5444 5445 5446 5448 10 GCGGACT 390 455 1266 1254 0.310.36 0.78 5442 5443 5444 5445 5446 5448 8389 10 ATTACTC 472 387 12661254 0.37 0.31 1.33 5442 5443 5444 5445 5446 5448 8389 10 ATGGACT 26 431266 1254 0.02 0.03 0.59 5442 5443 5444 5445 5446 5448 8389 10 GGACT 416498 1266 1254 0.33 0.40 0.74 5444 5445 5446 5448 8389 10 TACTC 472 3871266 1254 0.37 0.31 1.33 5444 5445 5446 5448 8389 10 ACT 441 522 12661254 0.35 0.42 0.75 5446 5448 8389 10 CTC 477 389 1266 1254 0.38 0.311.34 5446 5448 8389 10 ATC 7 17 1266 1254 0.01 0.01 0.40 5446 5448 838910 C 825 732 1266 1254 0.65 0.58 1.33 8389 10 T 441 522 1266 1254 0.350.42 0.75 8389 10 T 879 801 1266 1254 0.69 0.64 1.28 8390 10 C 387 4531266 1254 0.31 0.36 0.78 8390 12 GGG 31 61 1264 1264 0.02 0.05 0.50 56025603 5605 12 GCATT 294 245 1264 1264 0.23 0.19 1.26 5602 5603 5605 56065607 12 GGGCC 28 52 1264 1264 0.02 0.04 0.53 5602 5603 5605 5606 5607 12GCATTAG 294 245 1264 1264 0.23 0.19 1.26 5602 5603 5605 5606 5607 56085609 12 GGGCCAG 23 47 1264 1264 0.02 0.04 0.48 5602 5603 5605 5606 56075608 5609 12 T 422 352 1264 1264 0.33 0.28 1.30 5607 12 C 842 912 12641264 0.67 0.72 0.77 5607 12 TAG 419 348 1264 1264 0.33 0.28 1.31 56075608 5609 12 CAG 581 638 1264 1264 0.45 0.50 0.83 5607 5608 5609 12TTAGTCG 208 155 1264 1264 0.16 0.12 1.41 5606 5607 5608 5609 5611 56125613 12 TCAGTCG 8 20 1264 1264 0.01 0.02 0.40 5606 5607 5608 5609 56115612 5613 13 TAA 842 766 1258 1236 0.67 0.62 1.24 5728 5729 5730 13 CCG162 195 1258 1236 0.13 0.16 0.79 5728 5729 5730 13 A 860 773 1258 12360.68 0.63 1.29 5730 13 G 398 463 1258 1236 0.32 0.37 0.77 5730 13 C 940868 1258 1236 0.75 0.70 1.25 8392 13 A 318 368 1258 1236 0.25 0.30 0.808392 13 TAACCTCTT 28 10 1258 1236 0.02 0.01 2.79 5728 5729 5730 83925732 5733 5734 5735 5736 13 AACCTCTTC 27 10 1258 1236 0.02 0.01 2.695729 5730 8392 5732 5733 5734 5735 5736 8393 13 CTCTT 30 12 1258 12360.02 0.01 2.49 5732 5733 5734 5735 5736 13 TTCAT 82 58 1258 1236 0.070.05 1.42 5732 5733 5734 5735 5736 13 CTT 36 16 1258 1236 0.03 0.01 2.255734 5735 5736 14 T 804 869 1270 1254 0.63 0.69 0.76 5803 14 G 466 3851270 1254 0.37 0.31 1.31 5803 14 C 833 901 1270 1254 0.66 0.72 0.75 839414 T 437 353 1270 1254 0.34 0.28 1.34 8394 14 C 804 872 1270 1254 0.630.70 0.76 8395 14 A 466 382 1270 1254 0.37 0.30 1.32 8395 14 C 884 9481270 1254 0.70 0.76 0.74 5811 14 T 386 306 1270 1254 0.30 0.24 1.35 581114 T 874 932 1270 1254 0.69 0.74 0.76 5815 14 C 396 322 1270 1254 0.310.26 1.31 5815 14 A 901 970 1270 1254 0.71 0.77 0.71 5816 14 G 369 2841270 1254 0.29 0.23 1.40 5816 14 A 881 936 1270 1254 0.69 0.75 0.77 581714 G 389 318 1270 1254 0.31 0.25 1.30 5817 14 AAT 876 930 1270 1254 0.690.74 0.77 5817 8396 8397 14 GCC 364 281 1270 1254 0.29 0.22 1.39 58178396 8397 14 T 906 973 1270 1254 0.71 0.78 0.72 8397 14 C 364 281 12701254 0.29 0.22 1.39 8397 15 CTCTA 157 116 1272 1256 0.12 0.09 1.38 83985870 8399 5874 5880 15 CTA 191 149 1272 1256 0.15 0.12 1.31 8399 58745880 15 G 1076 1106 1272 1256 0.85 0.88 0.74 5880 15 A 196 150 1272 12560.15 0.12 1.34 5880 15 A 1074 1104 1272 1256 0.84 0.88 0.75 5882 15 C198 152 1272 1256 0.16 0.12 1.34 5882 15 CTA 185 144 1272 1256 0.15 0.111.31 5882 5885 5889 15 ATG 48 73 1272 1256 0.04 0.06 0.64 5882 5885 588915 A 1188 1143 1272 1256 0.93 0.91 1.40 5889 15 G 84 113 1272 1256 0.070.09 0.72 5889 16 T 654 587 1264 1266 0.52 0.46 1.24 5917 16 A 610 6791264 1266 0.48 0.54 0.81 5917 16 TAC 642 579 1264 1266 0.51 0.46 1.225917 5918 5921 16 AAC 446 525 1264 1266 0.35 0.41 0.77 5917 5918 5921 16TACTT 636 575 1264 1266 0.50 0.45 1.22 5917 5918 5921 5922 5923 16 AACTT163 220 1264 1266 0.13 0.17 0.70 5917 5918 5921 5922 5923 16 TACTTGA 635573 1264 1266 0.50 0.45 1.22 5917 5918 5921 5922 5923 5924 5925 16AACTTGA 163 218 1264 1266 0.13 0.17 0.71 5917 5918 5921 5922 5923 59245925 16 TACTTGATG 565 509 1264 1266 0.45 0.40 1.20 5917 5918 5921 59225923 5924 5925 5928 5930 16 AACTTGACG 15 38 1264 1266 0.01 0.03 0.395917 5918 5921 5922 5923 5924 5925 5928 5930 16 TACTTGATGTC 537 486 12641266 0.42 0.38 1.19 5917 5918 5921 5922 5923 5924 5925 5928 5930 84015933 16 AACTTGATGTT 94 126 1264 1266 0.07 0.10 0.73 5917 5918 5921 59225923 5924 5925 5928 5930 8401 5933 16 AACTTGACGTT 15 36 1264 1266 0.010.03 0.41 5917 5918 5921 5922 5923 5924 5925 5928 5930 8401 5933 16CTTGATGTC 589 534 1264 1266 0.47 0.42 1.20 5921 5922 5923 5924 5925 59285930 8401 5933 16 TGATGTC 701 630 1264 1266 0.55 0.50 1.26 5923 59245925 5928 5930 8401 5933 16 ATGTC 706 632 1264 1266 0.56 0.50 1.27 59255928 5930 8401 5933 16 GTC 706 634 1264 1266 0.56 0.50 1.26 5930 84015933 16 GTT 192 241 1264 1266 0.15 0.19 0.76 5930 8401 5933 16 C 779 7071264 1266 0.62 0.56 1.27 5933 16 T 485 559 1264 1266 0.38 0.44 0.79 593317 GGTCT 259 205 1270 1264 0.20 0.16 1.32 8403 8404 5973 8405 5980 17 G962 1006 1270 1264 0.76 0.80 0.80 5980 17 T 308 258 1270 1264 0.24 0.201.25 5980 17 TGC 240 188 1270 1264 0.19 0.15 1.33 5980 8406 8407 17 A930 975 1270 1264 0.73 0.77 0.81 8407 17 C 340 289 1270 1264 0.27 0.231.23 8407 18 ACGGA 49 71 1270 1262 0.04 0.06 0.67 6062 8409 8410 84116070 18 CGGAG 45 70 1270 1262 0.04 0.06 0.63 8409 8410 8411 6070 6071 18G 1200 1158 1270 1262 0.94 0.92 1.54 6070 18 A 70 104 1270 1262 0.060.08 0.65 6070 18 C 1111 1046 1270 1262 0.87 0.83 1.44 6071 18 G 159 2161270 1262 0.13 0.17 0.69 6071 18 A 1079 1005 1270 1262 0.85 0.80 1.446073 18 G 191 257 1270 1262 0.15 0.20 0.69 6073 18 G 1081 1007 1270 12620.85 0.80 1.45 8412 18 C 189 255 1270 1262 0.15 0.20 0.69 8412 19 AAACA622 677 1264 1264 0.49 0.54 0.84 6096 8414 8415 8416 8417 19 TAACA 2 101264 1264 0.00 0.01 0.20 6096 8414 8415 8416 8417 19 AACAC 622 680 12641264 0.49 0.54 0.83 8414 8415 8416 8417 6098 19 TGCTT 244 201 1264 12640.19 0.16 1.27 8414 8415 8416 8417 6098 19 A 624 688 1264 1264 0.49 0.540.82 8417 19 T 640 576 1264 1264 0.51 0.46 1.23 8417 19 ACA 622 681 12641264 0.49 0.54 0.83 8417 6098 6100 19 ACACC 618 670 1264 1264 0.49 0.530.85 8417 6098 6100 8418 6101 19 CACCA 619 672 1264 1264 0.49 0.53 0.856098 6100 8418 6101 6102 19 C 621 672 1264 1264 0.49 0.53 0.85 6101 19 G643 592 1264 1264 0.51 0.47 1.18 6101 20 CGG 521 452 1270 1262 0.41 0.361.25 6172 6175 6176 20 G 855 778 1270 1262 0.67 0.62 1.28 6176 20 A 415484 1270 1262 0.33 0.38 0.78 6176 20 GAT 809 740 1270 1262 0.64 0.591.24 6176 6179 6182 20 AGT 124 167 1270 1262 0.10 0.13 0.71 6176 61796182 20 AAC 13 27 1270 1262 0.01 0.02 0.47 6176 6179 6182 20 GATTC 739668 1270 1262 0.58 0.53 1.24 6176 6179 6182 6183 6184 20 AGTCT 106 1441270 1262 0.08 0.11 0.71 6176 6179 6182 6183 6184 20 AACCT 13 27 12701262 0.01 0.02 0.47 6176 6179 6182 6183 6184 20 T 778 715 1270 1262 0.610.57 1.21 6183 20 C 492 547 1270 1262 0.39 0.43 0.83 6183 20 TCT 778 7151270 1262 0.61 0.57 1.21 6183 6184 6185 20 CTG 492 547 1270 1262 0.390.43 0.83 6183 6184 6185 20 T 778 715 1270 1262 0.61 0.57 1.21 6185 20 G492 547 1270 1262 0.39 0.43 0.83 6185 20 TCTGCCG 523 440 1270 1262 0.410.35 1.31 6183 6184 6185 6186 6188 6189 6190 20 TGCCG 523 440 1270 12620.41 0.35 1.31 6185 6186 6188 6189 6190 21 TCG 111 163 1268 1266 0.090.13 0.65 6252 6254 6255 21 CAC 686 624 1268 1266 0.54 0.49 1.21 62546255 6256 21 CGC 217 278 1268 1266 0.17 0.22 0.73 6254 6255 6256 21 ACT629 564 1268 1266 0.50 0.45 1.23 6255 6256 6257 21 GCC 216 278 1268 12660.17 0.22 0.73 6255 6256 6257 21 T 994 924 1268 1266 0.78 0.73 1.34 625721 C 274 342 1268 1266 0.22 0.27 0.74 6257 21 CACTTGGAT 558 497 12681266 0.44 0.39 1.22 6254 6255 6256 6257 6258 6259 6260 6262 6264 21CGCCTACGT 43 64 1268 1266 0.03 0.05 0.66 6254 6255 6256 6257 6258 62596260 6262 6264 21 CACTTGGGC 14 34 1268 1266 0.01 0.03 0.40 6254 62556256 6257 6258 6259 6260 6262 6264 21 CACTTGGAC 8 1 1268 1266 0.01 0.008.03 6254 6255 6256 6257 6258 6259 6260 6262 6264 21 CTTGGAT 560 4971268 1266 0.44 0.39 1.22 6256 6257 6258 6259 6260 6262 6264 21 CCGGCGT33 52 1268 1266 0.03 0.04 0.62 6256 6257 6258 6259 6260 6262 6264 21CCTACGT 43 65 1268 1266 0.03 0.05 0.65 6256 6257 6258 6259 6260 62626264 21 CTTGGGC 14 34 1268 1266 0.01 0.03 0.40 6256 6257 6258 6259 62606262 6264 21 CTTGGAC 8 1 1268 1266 0.01 0.00 8.03 6256 6257 6258 62596260 6262 6264 21 G 1154 1097 1268 1266 0.91 0.87 1.56 6260 21 C 114 1691268 1266 0.09 0.13 0.64 6260 21 A 1085 1014 1268 1266 0.86 0.80 1.476262 21 G 183 252 1268 1266 0.14 0.20 0.68 6262 23 GCG 93 132 1270 12620.07 0.10 0.68 8428 8429 6409 23 GTA 191 152 1270 1262 0.15 0.12 1.298428 8429 6409 23 A 1167 1120 1270 1262 0.92 0.89 1.44 6409 23 G 103 1421270 1262 0.08 0.11 0.70 6409 23 C 1158 1115 1270 1262 0.91 0.88 1.368431 23 A 112 147 1270 1262 0.09 0.12 0.73 8431 23 CGAGACC 96 135 12701262 0.08 0.11 0.68 8429 6409 8431 8432 6411 8434 8435 23 TACGGCC 178134 1270 1262 0.14 0.11 1.37 8429 6409 8431 8432 6411 8434 8435 23 ACC111 149 1270 1262 0.09 0.12 0.72 6411 8434 8435 24 CAG 416 477 1266 12640.33 0.38 0.81 8436 8437 6676 24 CAC 367 317 1266 1264 0.29 0.25 1.228436 8437 6676 24 G 833 878 1266 1264 0.66 0.69 0.85 6676 24 C 433 3861266 1264 0.34 0.31 1.18 6676 24 CAGTTTTGA 27 47 1266 1264 0.02 0.040.56 8436 8437 6676 8440 8441 8442 8443 6679 6680 24 CAGCGTTCA 13 2 12661264 0.01 0.00 6.55 8436 8437 6676 8440 8441 8442 8443 6679 6680 24AAGCGTCCA 26 9 1266 1264 0.02 0.01 2.92 8436 8437 6676 8440 8441 84428443 6679 6680 24 CGTCG 280 332 1266 1264 0.22 0.26 0.80 8440 8441 84428443 6679 24 CGTTC 17 2 1266 1264 0.01 0.00 8.59 8440 8441 8442 84436679 24 CGTCC 58 33 1266 1264 0.05 0.03 1.79 8440 8441 8442 8443 6679 24GTCGG 92 122 1266 1264 0.07 0.10 0.73 8441 8442 8443 6679 6680 24 GTTCA17 2 1266 1264 0.01 0.00 8.59 8441 8442 8443 6679 6680 24 GTCCA 56 291266 1264 0.04 0.02 1.97 8441 8442 8443 6679 6680 24 G 957 1015 12661264 0.76 0.80 0.76 6679 24 C 309 249 1266 1264 0.24 0.20 1.32 6679 24CAT 281 227 1266 1264 0.22 0.18 1.30 6679 6680 8444 24 CCATCAG 57 291266 1264 0.05 0.02 2.01 8443 6679 6680 8444 6682 8445 8446 25 C 739 6531260 1252 0.59 0.52 1.30 8447 25 T 521 599 1260 1252 0.41 0.48 0.77 844725 CAA 416 337 1260 1252 0.33 0.27 1.34 8447 6721 6723 25 CAACC 201 1571260 1252 0.16 0.13 1.32 8447 6721 6723 8448 6728 25 TAACG 66 104 12601252 0.05 0.08 0.61 8447 6721 6723 8448 6728 25 ACG 139 174 1260 12520.11 0.14 0.77 6723 8448 6728 25 C 1056 1000 1260 1252 0.84 0.80 1.306728 25 G 204 252 1260 1252 0.16 0.20 0.77 6728 25 GTG 204 252 1260 12520.16 0.20 0.77 6728 6731 6732 25 GTGTT 166 223 1260 1252 0.13 0.18 0.706728 6731 6732 6733 6734 25 GTT 424 481 1260 1252 0.34 0.38 0.81 67326733 6734 26 T 865 916 1272 1264 0.68 0.72 0.81 6762 26 G 407 348 12721264 0.32 0.28 1.24 6762 26 T 857 803 1272 1264 0.67 0.64 1.19 6765 26 C415 461 1272 1264 0.33 0.36 0.84 6765 26 C 911 842 1272 1264 0.72 0.671.26 6767 26 T 361 422 1272 1264 0.28 0.33 0.79 6767 26 TTGTG 13 26 12721264 0.01 0.02 0.49 6766 6767 6769 6770 6772 26 CCCAC 28 14 1272 12640.02 0.01 2.01 6766 6767 6769 6770 6772 28 TGCTATG 96 136 1266 1262 0.080.11 0.68 8450 8451 8452 8453 8454 6914 6915 28 GCTATGG 96 136 1266 12620.08 0.11 0.68 8451 8452 8453 8454 6914 6915 6916 28 ATG 96 137 12661262 0.08 0.11 0.67 8454 6914 6915 28 A 1170 1125 1266 1262 0.92 0.891.48 6915 28 G 96 137 1266 1262 0.08 0.11 0.67 6915 29 TCGTG 120 1521264 1266 0.09 0.12 0.77 8457 8458 8460 8461 6979 29 GTA 854 794 12641266 0.68 0.63 1.24 8460 8461 6979 29 GTG 230 285 1264 1266 0.18 0.230.77 8460 8461 6979 29 A 1033 977 1264 1266 0.82 0.77 1.32 6979 29 G 231289 1264 1266 0.18 0.23 0.76 6979 29 GGC 228 288 1264 1266 0.18 0.230.75 6979 6980 8463 30 A 1113 1078 1254 1260 0.89 0.86 1.33 8465 30 T141 182 1254 1260 0.11 0.14 0.75 8465 30 AAA 811 747 1254 1260 0.65 0.591.26 8465 8466 8467 30 TAC 106 143 1254 1260 0.08 0.11 0.72 8465 84668467 30 A 1006 948 1254 1260 0.80 0.75 1.34 8467 30 C 248 312 1254 12600.20 0.25 0.75 8467 30 ACT 653 570 1254 1260 0.52 0.45 1.32 8467 70547056 30 CCA 197 262 1254 1260 0.16 0.21 0.71 8467 7054 7056 30 T 837 7521254 1260 0.67 0.60 1.36 7056 30 A 417 508 1254 1260 0.33 0.40 0.74 705630 C 810 887 1254 1260 0.65 0.70 0.77 7058 30 T 444 373 1254 1260 0.350.30 1.30 7058 30 CAC 473 544 1254 1260 0.38 0.43 0.80 7058 7059 8468 30TAC 442 371 1254 1260 0.35 0.29 1.30 7058 7059 8468 30 ACA 364 422 12541260 0.29 0.33 0.81 7059 8468 7061 30 ACG 551 493 1254 1260 0.44 0.391.22 7059 8468 7061 30 G 890 837 1254 1260 0.71 0.66 1.24 7061 30 A 364423 1254 1260 0.29 0.34 0.81 7061 31 GTC 285 349 1270 1260 0.22 0.280.76 8472 7103 7106 31 CTC 532 475 1270 1260 0.42 0.38 1.19 7103 71068473 31 TCT 195 240 1270 1260 0.15 0.19 0.77 7103 7106 8473 31 CCC 13 281270 1260 0.01 0.02 0.46 7103 7106 8473 31 C 1048 997 1270 1260 0.830.79 1.25 8473 31 T 222 263 1270 1260 0.17 0.21 0.80 8473 31 A 882 8141270 1260 0.69 0.65 1.25 7108 31 C 388 446 1270 1260 0.31 0.35 0.80 710832 GGATATA 39 19 1272 1264 0.03 0.02 2.07 8477 8478 8479 8480 8481 84828483 32 ATATA 40 22 1272 1264 0.03 0.02 1.83 8479 8480 8481 8482 8483 33CGCTT 11 3 1270 1258 0.01 0.00 3.66 8488 8489 8491 8492 8493 33 CGCTTTC11 3 1270 1258 0.01 0.00 3.66 8488 8489 8491 8492 8493 8494 7334 34 C340 435 1272 1262 0.27 0.34 0.69 8495 34 T 932 827 1272 1262 0.73 0.661.44 8495 34 A 370 467 1272 1262 0.29 0.37 0.70 7378 34 G 902 795 12721262 0.71 0.63 1.43 7378 34 AAC 365 464 1272 1262 0.29 0.37 0.69 73787379 7381 34 GAT 461 350 1272 1262 0.36 0.28 1.48 7378 7379 7381 34 C494 593 1272 1262 0.39 0.47 0.72 7381 34 T 778 669 1272 1262 0.61 0.531.40 7381 34 CCG 361 431 1272 1262 0.28 0.34 0.76 7381 7382 8498 34 CCA129 161 1272 1262 0.10 0.13 0.77 7381 7382 8498 34 TCG 167 116 1272 12620.13 0.09 1.49 7381 7382 8498 34 CCAAT 88 118 1272 1262 0.07 0.09 0.727381 7382 8498 8499 8500 34 TCGAT 119 72 1272 1262 0.09 0.06 1.71 73817382 8498 8499 8500 34 CCGGC 43 64 1272 1262 0.03 0.05 0.65 7381 73828498 8499 8500 34 TCGGC 20 9 1272 1262 0.02 0.01 2.22 7381 7382 84988499 8500 35 A 1176 1144 1262 1260 0.93 0.91 1.39 7437 35 G 86 116 12621260 0.07 0.09 0.72 7437 35 ATT 1174 1142 1262 1260 0.93 0.91 1.38 74377439 7440 35 GTG 7 19 1262 1260 0.01 0.02 0.36 7437 7439 7440 35 T 11811150 1262 1260 0.94 0.91 1.39 7440 35 G 81 110 1262 1260 0.06 0.09 0.727440 35 TTT 1066 999 1262 1260 0.84 0.79 1.42 7440 7441 7442 35 TTC 111146 1262 1260 0.09 0.12 0.74 7440 7441 7442 35 T 1069 1000 1262 12600.85 0.79 1.44 7442 35 C 193 260 1262 1260 0.15 0.21 0.69 7442 35 C 10711008 1262 1260 0.85 0.80 1.40 8501 35 T 191 252 1262 1260 0.15 0.20 0.718501 35 T 1056 995 1262 1260 0.84 0.79 1.37 7443 35 C 206 265 1262 12600.16 0.21 0.73 7443 36 AGCAC 121 90 1268 1262 0.10 0.07 1.37 7557 85048505 8506 8507 36 AGCTC 154 190 1268 1262 0.12 0.15 0.78 7557 8504 85058506 8507 36 CAC 491 428 1268 1262 0.39 0.34 1.23 8505 8506 8507 36 CTC159 194 1268 1262 0.13 0.15 0.79 8505 8506 8507 36 ACG 490 425 1268 12620.39 0.34 1.24 8506 8507 7561 36 TCG 185 222 1268 1262 0.15 0.18 0.808506 8507 7561 36 ACGCT 294 238 1268 1262 0.23 0.19 1.30 8506 8507 75618508 8509 36 TCGCT 179 219 1268 1262 0.14 0.17 0.78 8506 8507 7561 85088509 37 GTC 248 295 1264 1264 0.20 0.23 0.80 8513 8514 7613 37 GTG 851784 1264 1264 0.67 0.62 1.26 8513 8514 7613 37 C 410 472 1264 1264 0.320.37 0.81 7613 37 G 854 792 1264 1264 0.68 0.63 1.24 7613 37 TCG 824 7721264 1264 0.65 0.61 1.19 7619 7620 7621 37 A 437 488 1264 1264 0.35 0.390.84 7621 37 G 827 776 1264 1264 0.65 0.61 1.19 7621 40 GGA 138 105 12661256 0.11 0.08 1.34 7822 7823 7824 40 GAACC 257 301 1266 1256 0.20 0.240.81 7822 7823 7824 7825 7826 40 AGAAC 58 84 1266 1256 0.05 0.07 0.677822 7823 7824 7825 7826 40 GAAAC 16 2 1266 1256 0.01 0.00 8.03 78227823 7824 7825 7826 40 GGAACCA 36 17 1266 1256 0.03 0.01 2.13 7822 78237824 7825 7826 7827 7828 40 GAACCCG 123 166 1266 1256 0.10 0.13 0.717822 7823 7824 7825 7826 7827 7828 40 GGACATA 17 6 1266 1256 0.01 0.002.84 7822 7823 7824 7825 7826 7827 7828 40 GGAACCACC 34 16 1266 12560.03 0.01 2.14 7822 7823 7824 7825 7826 7827 7828 7829 7830 40 GAACCCGAT123 165 1266 1256 0.10 0.13 0.71 7822 7823 7824 7825 7826 7827 7828 78297830 40 GGACATACC 17 6 1266 1256 0.01 0.00 2.84 7822 7823 7824 7825 78267827 7828 7829 7830 40 AGAACCACC 5 17 1266 1256 0.00 0.01 0.29 7822 78237824 7825 7826 7827 7828 7829 7830 40 GAACCCGATTC 123 165 1266 1256 0.100.13 0.71 7822 7823 7824 7825 7826 7827 7828 7829 7830 8523 8524 40GGACATACCTT 15 3 1266 1256 0.01 0.00 5.01 7822 7823 7824 7825 7826 78277828 7829 7830 8523 8524 40 A 967 891 1266 1256 0.76 0.71 1.32 7828 40 G299 365 1266 1256 0.24 0.29 0.75 7828 40 C 971 893 1266 1256 0.77 0.711.34 7829 40 A 295 363 1266 1256 0.23 0.29 0.75 7829 40 CGATTCC 163 2281266 1256 0.13 0.18 0.67 7827 7828 7829 7830 8523 8524 8526 40 ATTCC 167229 1266 1256 0.13 0.18 0.68 7829 7830 8523 8524 8526 40 TCC 267 3331266 1256 0.21 0.27 0.74 8523 8524 8526 40 T 873 811 1266 1256 0.69 0.651.22 8526 40 C 393 445 1266 1256 0.31 0.35 0.82 8526 40 G 1044 964 12661256 0.82 0.77 1.42 7840 40 A 222 292 1266 1256 0.18 0.23 0.70 7840 42CTA 574 517 1262 1252 0.45 0.41 1.19 7909 7910 7911 42 TTA 71 102 12621252 0.06 0.08 0.67 7909 7910 7911 42 CTACA 125 83 1262 1252 0.10 0.071.55 7909 7910 7911 7912 7913 42 TTACG 61 93 1262 1252 0.05 0.07 0.637909 7910 7911 7912 7913 42 CTACAAC 106 78 1262 1252 0.08 0.06 1.38 79097910 7911 7912 7913 7915 7916 42 CTACAGC 13 3 1262 1252 0.01 0.00 4.337909 7910 7911 7912 7913 7915 7916 42 TTACGGC 26 59 1262 1252 0.02 0.050.43 7909 7910 7911 7912 7913 7915 7916 42 CTGCGGT 9 1 1262 1252 0.010.00 8.99 7909 7910 7911 7912 7913 7915 7916 42 ACGGC 155 192 1262 12520.12 0.15 0.77 7911 7912 7913 7915 7916 42 ACAAC 116 82 1262 1252 0.090.07 1.44 7911 7912 7913 7915 7916 42 ACAGC 13 4 1262 1252 0.01 0.003.25 7911 7912 7913 7915 7916 42 GCGGT 11 3 1262 1252 0.01 0.00 3.667911 7912 7913 7915 7916 42 G 1117 1146 1262 1252 0.89 0.92 0.71 7915 42A 145 106 1262 1252 0.11 0.08 1.40 7915 42 ACT 127 92 1262 1252 0.100.07 1.41 7915 7916 7918 43 T 553 498 1262 1260 0.44 0.40 1.19 7956 43 C709 762 1262 1260 0.56 0.60 0.84 7956 43 CTCAT 80 113 1262 1260 0.060.09 0.69 7956 7959 7962 7963 7965 43 TCACC 143 180 1262 1260 0.11 0.140.77 7959 7962 7963 7965 7966 43 TCATC 41 70 1262 1260 0.03 0.06 0.577959 7962 7963 7965 7966 44 A 510 575 1266 1264 0.40 0.45 0.81 8529 44 C756 689 1266 1264 0.60 0.55 1.24 8529 44 TATCCCG 105 139 1266 1264 0.080.11 0.73 8147 8529 8530 8531 8532 8533 8167 44 CATTATG 16 45 1266 12640.01 0.04 0.35 8147 8529 8530 8531 8532 8533 8167 44 TCCCG 181 227 12661264 0.14 0.18 0.76 8530 8531 8532 8533 8167 44 TTATG 17 45 1266 12640.01 0.04 0.37 8530 8531 8532 8533 8167 44 CCG 204 256 1266 1264 0.160.20 0.76 8532 8533 8167 44 CCT 449 400 1266 1264 0.35 0.32 1.19 85328533 8167 44 ATG 17 45 1266 1264 0.01 0.04 0.37 8532 8533 8167 44 G 237327 1266 1264 0.19 0.26 0.66 8167 44 T 1029 937 1266 1264 0.81 0.74 1.528167 44 GCT 121 180 1266 1264 0.10 0.14 0.64 8167 8534 8535 44 GCTGC 121179 1266 1264 0.10 0.14 0.64 8167 8534 8535 8536 8537 45 ACA 311 3691272 1264 0.24 0.29 0.78 8538 8231 8235 45 CAT 313 373 1272 1264 0.250.30 0.78 8231 8235 8236 45 TAT 94 67 1272 1264 0.07 0.05 1.43 8231 82358236 45 ATC 315 371 1272 1264 0.25 0.29 0.79 8235 8236 8539 45 C 378 4241272 1264 0.30 0.34 0.84 8539 45 T 894 840 1272 1264 0.70 0.66 1.19 853945 A 286 343 1272 1264 0.22 0.27 0.78 8239 45 G 986 921 1272 1264 0.780.73 1.28 8239 45 G 286 343 1272 1264 0.22 0.27 0.78 8241 45 A 986 9211272 1264 0.78 0.73 1.28 8241 47 CAG 31 16 1124 1130 0.03 0.01 1.97 85428543 8336 47 CAGTG 17 5 1124 1130 0.02 0.00 3.46 8542 8543 8336 83388340 47 C 332 386 1124 1130 0.30 0.34 0.81 8350 47 T 792 744 1124 11300.70 0.66 1.24 8350 47 C 330 385 1124 1130 0.29 0.34 0.80 8544 47 T 794745 1124 1130 0.71 0.66 1.24 8544 47 A 330 383 1124 1130 0.29 0.34 0.818545 47 G 794 747 1124 1130 0.71 0.66 1.23 8545

1. A method of constructing a longevity trait GeneMap in a humanpopulation, comprising screening for the expression level of or presenceor absence of at least one allele of (i) at least one gene from Tables4, 5 and 6 or (ii) at least one single nucleotide polymorphism (SNP)from Tables 2, 3 and 7 in at least one sample.
 2. A method of claim 1,wherein said population is a general population.
 3. A method of claim 1,wherein said population is a founder population.
 4. A method of claim 3,wherein said founder population is the population of Quebec. 5-21.(canceled)
 22. A set of genetic markers comprising at least two SNPs ofTables 2, 3 and
 7. 23. A set of nucleic acid probes that specificallydetect said SNPs of claim
 22. 24. A solid support or collection of solidsupports comprising the nucleic acid probes of claim
 23. 25. A solidsupport of claim 24, wherein the support is selected from the groupconsisting of at least one microarray and a set of beads.
 26. A methodfor predicting the efficacy of a drug for treating an age-associateddisorder in a human patient, comprising: a) obtaining a sample of cellsfrom the patient; b) obtaining (i) a gene expression profile or (ii) aset of genotype from the sample in the absence and presence of in vitromodulation of the cells with specific mediators; the gene expressionprofile comprising one or more genes from Tables 4, 5 and 6; the set ofgenotypes comprising one or more polymorphic loci from Tables 2, 3 and 7and c) comparing the gene expression profile or the set of genotypes ofthe sample with a reference gene expression profile or a reference setof genotypes associated with efficacy of the drug, wherein similaritybetween the sample expression profile and the reference expressionprofile or between the set of genotypes and the reference set ofgenotypes predicts the efficacy of the drug for treating age-associateddisorder in the patient.
 27. The method of claim 26, further comprisingexposing the sample to the drug for treating an age-associated disorderprior to obtaining the gene expression profile of the sample.
 28. Themethod of claim 26, wherein the sample of cells is derived from a tissueselected from the group consisting of: any parts of the body such as thescalp, blood, dermis, epidermis and other skin cells, cutaneoussurfaces, intertrigious areas, genitalia, vessels and endothelium. 29.The method of claim 28, wherein the cells are selected from the groupconsisting of: red blood cells, muscle cells, heart cells, nerve cells,insulin-producing cells, pancreatic cells, brain cells, germ cells,keratinocytes, monocytes, neutrophils, Langerhans cells, CD4+ and CD8+ Tcells, B and T lymphocytes, leukocytes, hormonal cells, bone marrowcells, skin cells, buccal cells, spinal cord cells, bone cells, adiposecells, cartilage cells, dendritic cells, intestinal cells, hepaticcells, mucous cells, olfactory cells, retinal cells, somatic cells andarterial cells.
 30. The method of claim 26, wherein the sample isobtained via biopsy.
 31. The method of claim 26, wherein the geneexpression profile comprises expression values for at least two of thegenes listed in Tables 4, 5 and
 6. 32. The method of claim 31, whereinthe gene expression profile of the sample is obtained by detecting theprotein products of said genes.
 33. The method of claim 26, wherein thegene expression profile of the sample is obtained using a hybridizationassay to oligonucleotides contained in a microarray.
 34. The method ofclaim 26, wherein the oligonucleotides comprises nucleic acid moleculesat least 95% identical to SEQ ID from Tables 2, 3, 4, 5 or
 7. 35. Themethod of claim 26, wherein the reference expression profile is that ofcells derived from patients that have the longevity trait.
 36. Themethod of claim 26, wherein the drug is selected from the groupconsisting of symptom relievers and drugs for an age-associatedcondition.
 37. A method for inducing a longevity-like state in aresident tissue or cell, comprising contacting the tissue or cell withat least one gene from Tables 4, 5 and 6 that induces a longevity-likestate.
 38. The method of claim 37, wherein the resident tissue cell isselected from the group consisting of red blood cells, muscle cells,heart cells, nerve cells, insulin-producing cells, pancreatic cells,brain cells, germ cells, keratinocytes, monocytes, neutrophils,Langerhans cells, CD4+ and CD8+ T cells, B and T lymphocytes,leukocytes, hormonal cells, bone marrow cells, skin cells, buccal cells,spinal cord cells, bone cells, adipose cells, cartilage cells, dendriticcells, intestinal cells, hepatic cells, mucous cells, olfactory cells,retinal cells, somatic cells and arterial cells.
 39. A method forscreening drug candidates for treating age-associated disorders,comprising: a) contacting a resident cell induced by the method of claim37 with a drug candidate for treating age-associated disorders; and b)assaying for a longevity-like state, such that an absence of thelongevity-like state is indicative of the drug candidate being effectivein treating age-associate disorders. 40-43. (canceled)
 44. A drugscreening assay comprising: a) administering a test compound to ananimal having an age-associated disorder, or related phenotype, or acell composition isolated therefrom; and b) comparing the level of geneexpression of at least one gene from Tables 4, 5 and 6 in the presenceof the test compound with one or both of the level of said geneexpression in the absence of the test compound or in normal cells;wherein test compounds which cause the level of expression of one ormore genes from Tables 4, 5 and 6 to approach normal are candidates fordrugs to treat age-associated disorders. 45-46. (canceled)
 47. A methodfor identifying a gene that regulates drug response in an age-associateddisorder, comprising: a) obtaining a gene expression profile for atleast one gene from Tables 4, 5 and 6 in a resident tissue cell inducedfor a longevity-like state in the presence of the candidate drug; and b)comparing the expression profile of said gene to a reference expressionprofile for said gene in a cell induced for the longevity-like state inthe absence of the candidate drug, wherein genes whose expressionrelative to the reference expression profile is altered by the drug mayidentifies the gene as a gene that regulates drug response in thelongevity trait.
 48. An expression profile indicative of the presence oflongevity trait in a patient, comprising the level of expression of atleast one gene of Tables 4, 5 and
 6. 49. A microarray comprising probesthat hybridize to one or more genes of Tables 4, 5 and
 6. 50-51.(canceled)
 52. A method of diagnosing susceptibility to anage-associated disorder in an individual, comprising screening for anat-risk haplotype of at least one gene from Tables 4, 5 and 6, orcomprising at least one SNP from Tables 2, 3 and 7, that is morefrequently present in an individual susceptible to an age-associateddisorder, compared to the frequency of its presence in a controlindividual, wherein the presence of the at-risk haplotype is indicativeof a susceptibility to an age-associated disorder.
 53. The method ofclaim 52, wherein the at-risk haplotype is characterized by the presenceof at least one single nucleotide polymorphism from Tables 2, 3 and 7.54. The method of claim 52, wherein screening for the presence of anat-risk haplotype in at least one gene from Tables 4, 5 and 6, orcomprising at least one SNP from Tables 2, 3 and 7, comprises enzymaticamplification of nucleic acid from said individual or amplificationusing universal oligos on elongation/ligation products.
 55. The methodof claim 54, wherein the nucleic acid is DNA.
 56. The method of claim55, wherein the DNA is human DNA.
 57. The method of claim 52, whereinscreening for the presence of an at-risk haplotype in at least one genefrom Tables 4, 5 and 6 or comprising at least one SNP from Tables 2, 3and 7 comprises: a) obtaining material containing nucleic acid from theindividual; (b) amplifying said nucleic acid as for claim 54; and c)determining the presence or absence of an at-risk haplotype in saidamplified nucleic acid.
 58. The method of claim 57, wherein determiningthe presence of an at-risk haplotype is performed by electrophoreticanalysis, restriction length polymorphism analysis, sequence analysis orhybridization analysis. 59-95. (canceled)
 96. A method for determiningthe phenotype of a cell comprising detecting the differentialexpression, relative to a normal cell, of at least one gene from Tables4, 5 and
 6. 97. The method of claim 96, wherein said difference in thelevel of expression of said gene, is of at least a factor of about two.98. The method of claim 96, including the further step of cloning saidgenes which are up- or down-regulated.
 99. The method of claim 96,including the further step of generating nucleic acid probes fordetecting the level of expression of said genes which are up- ordown-regulated.
 100. A kit for assessing a patient's risk of having ordeveloping an age-associated disorder, comprising: a) detection meansfor detecting the differential expression, relative to a normal cell, ofat least one gene shown in Tables 4, 5 and 6 or the gene productthereof; and b) instructions for correlating the differential expressionof said gene or gene product with a patient's risk of having ordeveloping an age-associated disorder.
 101. The kit of claim 100,wherein the detection means includes nucleic acid probes for detectingthe level of mRNA of said genes.
 102. A kit for assessing a patient'srisk of having or developing an age-associated disorder, comprising: (a)at least one means for amplifying or detecting a sequence of at leastone gene in Tables 4, 5 and 6, or at least one sequence comprising a SNPin Tables 2, 3 and 7, wherein the detection means includes nucleic acidprobes or primers for detecting the presence or absence of an associatedallele, a particular allele of a polymorphic locus, or the like orchanges to at least one sequence of Tables 4, 5 and 6 or Tables 2, 3 and7, and (b) instructions for correlating the presence or absence of atleast one sequence of Tables 4, 5 and 6 or Tables 2, 3 and 7 with apatient's risk of having age-associated disorder.
 103. The kit of claim102, wherein the detection means includes an immunoassay for detectingthe level of at least one gene product from Tables 4, 5 and
 6. 104. Amethod of assessing a patient's risk of having or developing anage-associated disorder, comprising: a) determining the level ofexpression of at least one gene from Tables 4, 5 and 6 or gene productsthereof, and comparing the level of expression to a normal cell; and b)assessing a patient's risk of having or developing an age-associateddisorder, if any, by determining the correlation between thedifferential expression of said genes or gene products with knownchanges in expression of said genes measured in at least one parentsuffering from an age-associated disorder.
 105. A nucleic acid arraycomprising a solid support comprising nucleic acid probes whichselectively hybridize to at least 5 different genes from Tables 4, 5 and6 or at least 5 different SNPs of Tables 2, 3 and
 7. 106. The array ofclaim 105, wherein the solid support is selected from the groupconsisting of paper, membranes, filters, chips, pins, and glass.
 107. Amethod of diagnosing the longevity trait in a patient, comprisingdetecting a nucleic acid molecule encoding at least one protein fromTables 4, 5 and 6 in a fluid or tissue sample from the patient.
 108. Amethod of claim 107, wherein the detecting comprises detecting at leastone associated allele, particular allele of a polymorphic locus, or thelike in the nucleic acid molecule encoding said protein.
 109. A methodof claim 107, wherein said method comprises hybridizing a probe to saidpatient's sample of DNA or RNA under stringent conditions which allowhybridization of said probe to nucleic acid comprising said associatedallele, a particular allele of a polymorphic locus, or the like, whereinthe presence of a hybridization signal indicates the presence of saidassociated allele, particular allele of a polymorphic locus, or thelike, in at least one gene from Tables 4, 5 and
 6. 110. A method ofclaim 109, wherein the patient's DNA or RNA has been amplified and saidamplified DNA or RNA is hybridized.
 111. A method of claim 109, whereinsaid method comprises using a single-stranded conformation polymorphismtechnique to assay for said associated allele, particular allele of apolymorphic locus, or the like.
 112. (canceled)
 113. A method of claim109, wherein said patient's sample of DNA has been amplified or cloned.114. A method of claim 109, wherein said method comprises sequencing atleast one gene from Tables 4, 5 and 6 in a sample of RNA or DNA from apatient.
 115. A method of claim 109, wherein said method comprisesdetermining the sequence of at least one gene from Tables 4, 5 and 6 bypreparing cDNA from RNA taken from said patient and sequencing said cDNAto determine the presence or absence of an associated allele, aparticular allele of a polymorphic locus, or the like.
 116. A method ofclaim 109, wherein said method comprises performing an RNAse assay. 117.A method of claim 109, wherein said probe is attached to a microarray ora bead.
 118. A method of claim 109, wherein said probes areoligonucleotides.
 119. A method of claim 109, wherein said sample isselected from the group consisting of blood, normal tissue and tumortissue.
 120. A method of claim 109, wherein the associated allele,particular allele of a polymorphic locus, or the like is selected fromthe group consisting of at least one of the SNPs from Tables 2, 3 and 7,alone or in combination. 121-123. (canceled)
 124. A method fordiagnosing or prognosticating longevity comprising comparing the levelof expression or activity of a polypeptide encoded by a gene of Tables4, 5 and 6 in a test sample from a patient with the level of expressionor activity of the same polypeptide in a control sample wherein adifference in the level of expression or activity between the testsample and control sample is indicative of the longevity trait. 125-131.(canceled)
 132. The method of claim 26, wherein the set of genotypesfrom the sample comprises genotypes of at least two of the polymorphicloci listed in Tables 2, 3 and
 7. 133. The method of claim 26, whereinthe set of genotypes from the sample is obtained by hybridization toallele-specific oligonucleotides complementary to the polymorphic locifrom Tables 2, 3 and 7, wherein said allele-specific oligonucleotidesare contained on a microarray.
 134. The method of claim 133, wherein theoligonucleotides comprise nucleic acid molecules at least 95% identicalto SEQ ID from Tables 1, 2 or
 3. 135. The method of claim 26, whereinthe set of genotypes from the sample is obtained by sequencing saidpolymorphic loci in said sample.
 136. The method of claim 26, whereinthe drug is selected from the group consisting of symptom relievers anddrugs for an age-associated condition. 137-145. (canceled)
 146. A kitfor assessing a patient's risk of having or developing an age-associateddisorder, comprising: a) detection means for detecting the genotype ofat least one polymorphic locus shown in Tables 2, 3 and 7; and b)instructions for correlating the genotype of said at least onepolymorphic locus with a patient's risk of having or developing anage-associated disorder.
 147. The kit of claim 146, wherein thedetection means includes nucleic acid probes for detecting the genotypeof said at least one polymorphic locus.
 148. A method of assessing apatient's risk of having or developing an age-associated disorder,comprising: a) selecting at least one polymorphic locus from Tables 2, 3and 7; b) determining a genotype for said at least one polymorphic locusfrom Table 1 in a patient; c) comparing said genotype of b) to agenotype for said at least one polymorphic locus from Tables 2, 3 and 7that is associated with the age-associated disorder; and d) assessingthe patient's risk of having or developing the age-associated disorder,wherein said patient has a higher risk of having or developing theage-associated disorder if the genotype for said at least onepolymorphic locus from Tables 2, 3 and 7 in said patient is the same assaid genotype for said at least one polymorphic locus from Tables 2, 3and 7 that is associated with the age-associated disorder.